Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 523,335 | 0 | T | G | 51.0% | 50.8 / 72.3 | 51 | G25G (GGT→GGG) | rhsD | Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor |
Reads supporting (aligned to +/- strand): ref base T (8/17); new base G (26/0); total (34/17) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.32e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-06 |
GGTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTC > NC_000913/523188‑523484 | ggTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCACTTTCCCGGGGTTGGCAGGGGTa > 2:932681/1‑151 (MQ=255) cTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTGTCCCTGGGTCGGCCGGGGTAAG‑a > 2:230563/1‑151 (MQ=255) ttGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑aa < 2:397271/151‑1 (MQ=255) ttGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑aa < 1:1114962/151‑1 (MQ=255) tgtgtgTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTGGGGAGGTGTTAG‑AAt > 2:1097205/1‑151 (MQ=255) gtCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTTTCCAGGGGTTGGCCGGGGGAAG‑AAATgggg > 1:270004/1‑149 (MQ=255) tCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGgcg < 2:579350/150‑1 (MQ=255) tgttgCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCggcgtggcg < 2:406833/151‑1 (MQ=255) gCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTTCCATTGTCCAGGGGTCGGCAGGGGTAAG‑AATTGGCGCGCCCACCGGCGCGGCGTGc > 1:1006658/1‑150 (MQ=255) gCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGGGTAAG‑AAATGGCGCGGCCCCCggcggtgcgggc > 2:319789/1‑142 (MQ=255) cacaGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGGAAG‑AATTGGCGCGCCCACCGGCGGGGCGTGCTCgggg > 1:327722/1‑149 (MQ=255) gggATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCCGGGGTCGGCAGGTGTAAG‑AAATTGCGCGCCCACCGGGCGGGCCTGCTCGgtgtgtt > 2:914735/1‑150 (MQ=255) gggATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCCGGGGTAAG‑AATTTGCGCGCCCACCGGCGTGGCGTGCTCgggggtgt > 1:1195459/1‑145 (MQ=255) gggATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCCGGGGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGGGCTCGgtgtgt > 1:637216/1‑150 (MQ=255) gggATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGgtg < 2:1194971/147‑1 (MQ=255) gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTTCCATTTTCCAGGGGTCGGCAGGGGGAAG‑AATTGGGGGGCCCACCGGCGTGGGGTGCTCggggggggcg > 2:493480/1‑143 (MQ=255) gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTTCCAATTTCCAGGGGTTGGCCGGTGTAAG‑AATTGGCGCGCCCCACGGCGGGGCGTGCTCGGGGTGT‑Ccg > 1:1023713/1‑151 (MQ=255) gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTGTCCAGGGGTCGGCCGGGGTAAA‑AATTGGCGCGCCCACCGGCGGGGCGGGGTCGGGGTGG‑Ccg > 1:268312/1‑151 (MQ=255) gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGGGGAAG‑AATTTGCGCGCCCCCCGGCGTGGCGTGCTCGGTGGGT‑Tcg > 1:147482/1‑151 (MQ=255) gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGTAAG‑AATTTGCGCGCCCACCGGCGTGGCGGGCCCggggggttcg > 1:826406/1‑143 (MQ=255) gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTACCCTTGTCCACGGTTCCGCAGGGGGAAG‑AATTTTGGGGCCCACCGGCGGGGCGGGCTCCGGGGGT‑Ccg > 2:1054841/1‑151 (MQ=255) gATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGGTCCATTGTCCCGGGGTCGGCAGGGGTAAG‑AAATTGCGCCCCCCCCCGCGTGGCCCGCCCGGGGGGT‑Ccg > 2:37498/1‑151 (MQ=255) gAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGGGTAAG‑AAATTGCGCGCCCCCCGGCGGGGGGGGCTCGGGGTGG‑Ccgggggg > 1:48961/1‑151 (MQ=255) aaCAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGGAAG‑AATTGGCGCGCCCACCCGCGTGGCGGGCCCGGGGGGT‑Ccgggcggg > 1:228173/1‑151 (MQ=255) aTAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCTTGTCCAGGGGTCGGCAGGGGTGAG‑AAATGGCGCGCCCCCCCGCGTGGCGGGCTCGGTGGGTGCcgggggggtgg > 2:1005430/1‑148 (MQ=255) cTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCCGGGGTAAG‑AATTGGCGCGCCCCCCGGCGTGGCGTGCTCGGGGTGT‑Gcgggcg > 2:10909/1‑141 (MQ=255) cTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑Ccgggcg < 1:10912/141‑1 (MQ=255) aaGGTCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGc < 2:1023341/151‑1 (MQ=255) cTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCCCTCTTCACGGTTCGGGCGGGGGAAGCAATTGG‑GTGCCCACCCGCGTTGCCTGCCCGGGGTGT‑GCGGGGTGGGGGCCGTCGGGCAACACgg > 2:706273/1‑151 (MQ=255) cTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCCGGGGGAAG‑AATTGGCGCGCCCACCGGCGTGGGGGGCTCCGGGTGT‑CCGGGCGGGGGGACTTCGGGGCAccccg > 2:1008246/1‑149 (MQ=255) cTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGTAAG‑GAATGGGGCGCCCACCGGCGGGGCGTGCTCGGTTGGT‑CCGGGCGGGGTGGCTTCTGGCAAccccg > 2:187935/1‑149 (MQ=255) cTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGGTCGGGGTGT‑CCGGGCGGGGTGGCTTCGGGCAACCCgg > 1:144482/1‑151 (MQ=255) ttATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTTTCCAGGGGTCGGCAGGGGTAAG‑AATTGGGGCGCCCACCGGCGTGGCGGGGTCGGGGTGT‑CCGGGGGGGGTGACTTCGGGCAACCCggga > 1:318514/1‑149 (MQ=255) tataTGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTaa < 2:790232/151‑1 (MQ=255) tataTGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTaa < 1:1126432/151‑1 (MQ=255) atGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATc < 2:716752/151‑1 (MQ=255) cggcgCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTAGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCg < 1:983372/149‑1 (MQ=255) cgcgTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGt < 1:614285/151‑1 (MQ=255) tCAGGGAGATATGACTCAGTATGGCGGTCCCATTTTCCAGGGTTCCGCAGGGGTAAG‑AATTGGCGCGCCCCCCGGCGTGGGGTGGGCGGTTTGT‑TCCGGCGGGGTGGCTTCGGGCAACCCGGGAAATCCCCTGCTGGGGGGGGAgggg > 2:29077/1‑146 (MQ=255) tCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGGAGGGGGAAG‑AATTGGCGCGCCCCCCGGCGTGGCGGGCTCGGGGTGT‑CCGGGCGGGGTGACTTCGGGCAACCCCGGAAATCCGCTGCCGGGGGCGGAgggg > 1:279667/1‑146 (MQ=255) gaTATGACTCAGTATGGCGGTCCCCTTGTCCAGGGGTCGGCCGGTGTAAG‑AATTTGCGCGCCCCCCGGCGTGGCGGGCTCGGGGTGG‑TCGGGGGGGGTGACTTCGGGCCACCCGGGAAAACCCCTGCTGGGGGCGGAGGGGCGGCCCcgcg > 2:537124/1‑151 (MQ=255) gaTATGACTCAGTATGGCGGTCCCATTGTCCAGGGGTCGGCAGGGGTAAA‑AATTGGCGGGCCCACCGGCGGGGCGTGCTCGGTGGGT‑CCGGGCGGGATGACTTCGGGCAACCCGGGAAATCCGCCGCTGGGGGGGAAGGGGCTGCCCGGCg > 1:1000781/1‑151 (MQ=255) tCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGAc < 2:332656/151‑1 (MQ=255) gCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTgcgc < 1:432584/150‑1 (MQ=255) gCGGTCCCATTGTCCAGGGCTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTgcgc < 1:1267588/150‑1 (MQ=255) cATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccgg > 1:1059418/1‑150 (MQ=255) ttGTCCAGTGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTgcccggccc < 2:826080/151‑1 (MQ=255) gTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGCGACGGACCTTGCGCTgcccggcccg > 1:1231212/1‑150 (MQ=255) gggTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGGGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCg > 2:486449/1‑151 (MQ=255) gTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGtt > 2:945503/1‑151 (MQ=255) cAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtc < 1:638245/150‑1 (MQ=255) gtgtAAG‑CATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGCAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGCCCTTGCGCTGCCCGGCCCGCTGCCGGTCCTtcccc > 2:164265/1‑147 (MQ=255) gtgtAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctct > 1:957043/1‑150 (MQ=255) gtgtAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctct > 1:84558/1‑150 (MQ=255) gtgtAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctct > 1:100590/1‑150 (MQ=255) tgtAAG‑AATTGGCGCGCCCCCCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctctc < 1:673391/150‑1 (MQ=255) tgtAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATtctctc < 1:224202/150‑1 (MQ=255) | GGTCTTGTGTGTCATTTTATTTTTTTTTGTTGCTAACACAGGGATATGAACAATAACTAAAAGGGCACTTTATATGAGCGGAAAACCAGCGGCGCGTCAGGGAGATATGACTCAGTATGGCGGTCCCATTGTCCAGGGTTCGGCAGGTGTAAG‑AATTGGCGCGCCCACCGGCGTGGCGTGCTCGGTGTGT‑CCGGGCGGGATGACTTCGGGCAACCCGGTAAATCCGCTGCTGGGGGCGAAGGTGCTGCCCGGCGAGACGGACCTTGCGCTGCCCGGCCCGCTGCCGTTCATTCTCTC > NC_000913/523188‑523484 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |