Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA44.4% 62.1 / 35.6 54intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (26/4);  new base A (0/24);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.48e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGA  >  NC_000913/29346‑29643
                                                                                                                                                         |                                                                                                                                                   
tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                     <  2:403406/151‑1 (MQ=255)
       tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                              <  1:871528/151‑1 (MQ=255)
         aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATcc                                                                                                                                            <  1:482393/151‑1 (MQ=255)
                    tttGTTCCTTTTTTTTTTGTTCTTTTTTTTTTAATGCATTTTTCTGGCTGCCAAGGCGTTTCCCAAAACAGTTAAGTTAATTTTTTTTTCGTTTATTCCTTGTAAAAAAGACATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGt                                                                                                                                 <  2:70500/151‑1 (MQ=255)
                        tcccATTTTTTTCTGCTGTTTTTTTTTTCATGCAATCTTCTTGCTGCGAAAGCGTTTTCCAGAACAGGTTAGATAATTTTTTTGTCGTTTAATCCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGa                                                                                                                              <  1:355371/148‑1 (MQ=255)
                        gTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGa                                                                                                                              >  2:1250540/1‑150 (MQ=255)
                        gTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGa                                                                                                                              >  1:1007503/1‑150 (MQ=255)
                              ttttttttGCTCTTTTTTTTTTCATCAATTTTTCTTCCGGCGAAAGTTTTTCCAAGAAAAGTTAAAATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCAGGAGCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                                       <  1:281463/151‑1 (MQ=255)
                               tttttCTGCTCGTTTTTATTTCATGAAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                                       >  2:884990/1‑150 (MQ=255)
                                        aagTTTTTATTTCATGAAATTTTTTTGCTGGAAAAGCTTTTCCCAGAACAGGTAAGATGATCTTTTTTTCGTATATGGCCTAAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtc                                                                                                             <  2:41473/149‑1 (MQ=255)
                                          gTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                            >  2:1022940/1‑150 (MQ=255)
                                           tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATTAGTCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                           >  2:218009/1‑150 (MQ=255)
                                           tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                           >  2:1249927/1‑150 (MQ=255)
                                           tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtc                                                                                                             >  1:41479/1‑148 (MQ=255)
                                           tttttATTTCATGCAATCTTCTTGCCGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                           >  2:296222/1‑150 (MQ=255)
                                             ttttttTCATGCATTTTTTTTGCTGCGAAGGCGTTTCCCAGAAAAGTTTAGATGATTTTTTTTTCGTTTAATGCCTTTAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                        <  1:267182/151‑1 (MQ=255)
                                                ttttCATGCATTTTTTTTCCCCCGCAAGGTTTTCCCAAAACGTGTAAAATGATTTTTTTTTTGTTTAATCCCTGAAAAACTGGCAGGTCCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                     <  1:296290/150‑1 (MQ=255)
                                                       gCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGTATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                              >  2:875184/1‑151 (MQ=255)
                                                       gCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                              >  1:742765/1‑151 (MQ=255)
                                                          aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTTACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                           >  1:1145226/1‑151 (MQ=255)
                                                          aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                           >  1:97144/1‑151 (MQ=255)
                                                                   ctcccaaaGCTTTTCCAAAAACAGGTTAGATAATTTTTTTGTCGTTAAATGCCTGAAAAAAATGCATGACCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                                   <  1:347488/144‑1 (MQ=255)
                                                                             cttttCCAAGAACAGTTTAGATAATTTTTTTGCCCTTTAAGGCCTAAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                          <  1:255850/149‑1 (MQ=255)
                                                                                ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  2:280825/1‑151 (MQ=255)
                                                                                ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  1:128600/1‑151 (MQ=255)
                                                                                ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  2:936376/1‑151 (MQ=255)
                                                                                   caaaaacggtttAGATAATTTTTTTGCCGTTAAATCCATAAAAAAAATGCAGGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                   <  2:881035/141‑1 (MQ=255)
                                                                                    caaaaaaGGTTAAGATGATTTTTTTTCCCTTAAAGCCTTAAAAAACATGAATGCCCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                   <  1:642482/145‑1 (MQ=255)
                                                                                         cAGGTTAGCTGATCTTTTTGTCTCTTAATGCCCGTACAACATGCATGAGCCACAAGATAATATAAAAAATCCCGCCATTCAGCTGACTTTTATCGCCCATATCTCCAGAATGCCGCCGTTTGCAAGAAATTCGGCGGTAAGCAGATTTGCa                                                             >  2:687483/1‑151 (MQ=255)
                                                                                           ggTTAGATGATCTTTTTGTCGCTTCATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGCATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                           >  2:841370/1‑151 (MQ=255)
                                                                                           ggTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                           >  2:399483/1‑151 (MQ=255)
                                                                                           atttaagattttttttttGTCGCTTAATGCTTGTAAACCATGAATGCCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                            <  1:452011/138‑1 (MQ=255)
                                                                                            gttaatatgttttttttGTCCTTAAAGGCCTAAAAAACTGGCATGAGCAAAAAAAAAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                            <  2:853973/138‑1 (MQ=255)
                                                                                                 gatgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                      >  1:349587/1‑151 (MQ=255)
                                                                                                   ttatttttttGTCGTTTAATGCCAGAAAAACATGCATGAGCCACAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                                    <  2:1066026/146‑1 (MQ=255)
                                                                                                     ttttttttgcccttaaaTGCCTGAAAAAAATGCAGGCACCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  <  1:399595/136‑1 (MQ=255)
                                                                                                              ttcgtttaatcctgatAAAACTTGCAGGAACAAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                          <  2:176931/136‑1 (MQ=255)
                                                                                                              tcgataaaTGCCTTAAAAACAGGCTGGACACAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                          <  2:706425/144‑1 (MQ=255)
                                                                                                                cgctaaatccttaaaaaaCATGCATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                        <  1:1019013/137‑1 (MQ=255)
                                                                                                                     aTGCATGAAAAACTTGAAGGCCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                  <  1:144612/151‑1 (MQ=255)
                                                                                                                        ccTGTAAAACATGCATGAGCCACAAAATTATATAAAAAATCCCTCCTTTAAGATGACTTTTAGGGCCTATATCTCCAGAATGCCGTCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGACTTACGTCATCATTGTGAATTAatat                               >  2:861114/1‑151 (MQ=255)
                                                                                                                        ccTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                               >  2:606545/1‑151 (MQ=255)
                                                                                                                            gTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                            >  2:1092235/1‑151 (MQ=255)
                                                                                                                            gTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                            >  1:756206/1‑151 (MQ=255)
                                                                                                                                 aCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                       >  1:407767/1‑151 (MQ=255)
                                                                                                                                 aCATGCAGGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                       <  2:1215002/151‑1 (MQ=255)
                                                                                                                                   atgcatgcGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                     <  1:1140693/151‑1 (MQ=255)
                                                                                                                                      tagaggcaaaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                   <  2:1193913/140‑1 (MQ=255)
                                                                                                                                       atggccaaaaaaaTAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                 <  1:111181/142‑1 (MQ=255)
                                                                                                                                       atgACCCAAAAAATAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                 <  2:30404/151‑1 (MQ=255)
                                                                                                                                       atgACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                 <  1:1251804/151‑1 (MQ=255)
                                                                                                                                          aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga              >  1:448056/1‑151 (MQ=255)
                                                                                                                                           cccAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa             <  1:646049/150‑1 (MQ=255)
                                                                                                                                           aacaaaaaaaaaaaaaaaaaaTCCCGCCATTAAGTTGACTTTTAACGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa             <  1:641878/146‑1 (MQ=255)
                                                                                                                                             cacaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata           >  1:571018/1‑151 (MQ=255)
                                                                                                                                                aaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc        <  1:1251065/151‑1 (MQ=255)
                                                                                                                                                      atatAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGa  >  2:782191/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                   
TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGA  >  NC_000913/29346‑29643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: