Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA52.0% 62.2 / 152.3 99A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (38/9);  new base A (0/51);  total (38/61)
Fisher's exact test for biased strand distribution p-value = 6.29e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-11

GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAT  >  NC_000913/3665553‑3665851
                                                                                                                                                        |                                                                                                                                                         
gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                                          >  2:648337/1‑151 (MQ=255)
  gatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaa                                                                                                                                                        >  1:735160/1‑151 (MQ=255)
     gaACGAGTAATGAATAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACTCACTTATCTACTCGAATTTGGCTTGCATCCGCaaaaa                                                                                                                                                      >  2:1048814/1‑150 (MQ=255)
           gTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAACTTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGt                                                                                                                                              >  2:624198/1‑150 (MQ=255)
                      aCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaa                                                                                                                                >  1:1229192/1‑151 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  2:584707/1‑151 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  2:904098/1‑151 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  2:31763/1‑151 (MQ=255)
                         tttttATGTGAATGAAGTTTTAATGCCTCCTCCTTGAACACGGGGAAAAAAAATAAAAGCGTGTCCCTTGACACAAATAAAAAATTTTCAACACCCTTATCTCCTCGAATTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAAt                                                                                                                             <  1:261233/151‑1 (MQ=255)
                           tttATGTGCATAAAGTTTTAATGCCTCCTCCTTGAGCATGGGAAAAAAAAATGAAAGCGTGTCCCTTGACACAAAAAAAAATTTTTCAACACTTTTATTTCATCAATTTTGGCTTCAACCCGAAAAAACAAGGTCACCGCCATTAAAATAg                                                                                                                           <  2:1162669/151‑1 (MQ=255)
                           ttaaTGTGCTAGAAGATTTAATCCCTCCCCCTTAAGCAGCGGGAAAAAAAATGAAAGCGTGTCCCTTGACACAAATACAAAATTTCCACCCCCCTTATCAACAAGAATTTGGCTTGCACCCGAAAAAACCGGGTCACCGCCATTAAAATAg                                                                                                                           <  2:402790/148‑1 (MQ=255)
                                  gCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                    >  2:710487/1‑151 (MQ=255)
                                     taaaGTTTAAATCCCCCCTCCTTGACAATCGGAAAAAATAATAAAAGCGTGTCCCTTGACCCGAAAACAAAATTTCCACCTCCCTTATCTACCTGATTTTGTCTTCCATCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCAc                                                                                                                 <  2:891422/149‑1 (MQ=255)
                                     tGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCa                                                                                                                  >  2:90364/1‑150 (MQ=255)
                                      aaaaaTTTAATGCCCCCCCCTTGAGAATCGGGAAAAAAAATGAAAGCGTGTCCCTTGACACAAATAAAAAATTTTCAACACCCTTATCTACTCAAATTTGGCTTGCACCCGAAAAAACCAGGTCCCCGCCATTAAAATAGCGATATTCAcc                                                                                                                <  1:304610/147‑1 (MQ=255)
                                         gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGCATACAAAATTTTCAACACACTTATCTACTCGACTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                             >  2:1023557/1‑151 (MQ=255)
                                          aTTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                            >  1:462843/1‑151 (MQ=255)
                                          aTTTAATCCCTCCCCCTTGAGCATGGGGAAAAAAAATAAAAGCGTGCCCCTTGACAAGAAAAAAAAATTTTCAACACACTTATCTACTCAAATTGGGCTTGCCCCCGAAAAACCCAGGTCCCCGCCATTAAAATAGCGATATTCACCAt                                                                                                              <  1:36055/149‑1 (MQ=255)
                                           tttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTaa                                                                                                           >  2:1151091/1‑151 (MQ=255)
                                           tttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTaa                                                                                                           >  1:481534/1‑151 (MQ=255)
                                            ttAATGCCTCCTCCTGGAGCATCGGGAAAAATAATGAAAGCGTGTCCTTTGACACGAATAAAAAATTTTCAACACACTTATCTTCAAGAATTGGGCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                          <  2:885783/151‑1 (MQ=255)
                                             tattGCCCCCTCCTTGAGCAGCGGAAAAAATAATGAAGGCGTGTCCGTTAACAAGAATAAAAAATTTTCAACACCCTTATCTCCTCAATTTTGGTTGGCATCCAAAAAACCCGGTGCCCCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:170679/148‑1 (MQ=255)
                                             taagccctccccctTGAGCATCGGGAAAAATAATAAAAGCGTCCCCCTTAACACAAATACAAAATTTTCAACCCATTTATTAACTAAATTTTGCCTTCCACCCGCAAAACCAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:46322/146‑1 (MQ=255)
                                             tAATGCCTCCTCCGTGAGAATGGGAAAAAAAAAGAAAAGCTTGTCCCTTGAAACGAATAAAAAATTTCCAACACTCTTATATACACGATTTGGGCTTGCACCCAAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:766753/151‑1 (MQ=255)
                                             tAATGCCCCCTCCTTGAGCATCGGGAAAAATAATGAAGGCGTGCCCTTGGACAAGAATAAAAAATTTCCAACCCATTTATCTCCTCAATTTGGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:557069/151‑1 (MQ=255)
                                             tAATGCCCCCCCCTTGACCATCGGGAAAAATAATGAAAGCGTGTCCCTTAACACAAATAAAAAATTTTCAACACCCTTATCTACTCGATTTTGGCTTGCACCCCAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:1257865/151‑1 (MQ=255)
                                             tAATCCTTCTTCCTTGACCATGGTGAAAAAGAAGGAAGGCTTATCCTTAAACACTAATACAAAATTTTAAACCCACTTATCTATTCGTATTGGGCTTGCCCCCAAAAAACCCTGTCTACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:732103/151‑1 (MQ=255)
                                             tAATCCCTCCTCCTTAACCATCGGAAAAAAAAATGAAGGTTTTTCCCTTACCACAAATAAAAATTTTCCAACACCCTTTTCTACCCAATTTGGGCTTGCACCCAAAAAAACCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:947743/151‑1 (MQ=255)
                                             tAATCCCCCCTCTTGGAGCACGGGGAAAAAAAATAAAAGCGTGCCCTTTGACAAAAAAAAAAAATTTCAAACACATTTATCTACTAAATTTTGGCTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:60255/151‑1 (MQ=255)
                                             tAATCCCCCCTCCTTGAGCATGGGGAAAAAAAATAAACGCGTCTCCTTTGACACAAAAAAAAAATTTTCAACCCCCTTACCAACTAGAATTGGGTTCCCACCCGAAAAACCCGGTTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:589251/151‑1 (MQ=255)
                                             tAATCCCCCCTCCTGGAGCATGGGGAAAAAAAATAAAAGCGTTCCCCTTGACAAAAATAAAAAATTTTCAACACACTTATCTTCTCGATTTTGGCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:398747/151‑1 (MQ=255)
                                             tAATCCCCCCCCCTTGAGCATCGGGAAAAATAAAGAAAGCGTGCCCCTTGACAAAAATAAAAAATTTTCAACACATTTATCTCCTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:370462/151‑1 (MQ=255)
                                             aaaTCCCTCCTCTTGGACCATCGGGAAAAAAAATGAAAGCGTTCCCTTTGACACAATTACAAAATTTCCACCCCCCTTATCTTCTCAATTTTGCCTTCCACCCGCAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:96577/150‑1 (MQ=255)
                                               atccctcctcctTGAGCATGGGGAAAAAAAATGAAGGCGTGCCCCTTGACACAAAAAAAAAATTTTCAACCCACTTATCTACCCAAATTTGGCTTGCATCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                                       <  1:1070638/148‑1 (MQ=255)
                                                  cctcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                    >  2:280085/1‑151 (MQ=255)
                                                   cccctcttGGAGCATCGGGAAAAAAAATGAAAGCGTGCCCCTTGACACAAAAAAAAAATTTACAACACTCTTATCTCCGCAAATTTGGCTTGCACCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                   <  1:901161/149‑1 (MQ=255)
                                                    tcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                  >  1:53928/1‑151 (MQ=255)
                                                       tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                               >  2:802349/1‑151 (MQ=255)
                                                       tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                               >  2:233722/1‑151 (MQ=255)
                                                        cctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                              >  1:521964/1‑151 (MQ=255)
                                                             accATGGGGAGAAAAAATAAAGGCGTGCCCCTGGACAAAAATAAAAATTTTTAAACACACTTATCTACTCGATTTTGGTTTGCACCCAAAAAACCAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:1013059/149‑1 (MQ=255)
                                                             accATCGGAAAAAATAAAGAAAGCTTGTCCTTGGCCACAAAAAAAAAATTTAAAACACACTTATCTACGCGAATTTGCTTAGCATCCGAAAAACCCAGGCAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:847636/149‑1 (MQ=255)
                                                              caaaggggAAAAAAAATAAAAGCTTTCCCTTTGACCCAAATACAAAATTTCCACCCCATTTTTCTCCTCAATTTTGGCTTGCACCCAAAAAAACCAGGGCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:398025/145‑1 (MQ=255)
                                                              acATCGGAAGAAATAATAAAAGCGTCTCCTTTGACACAAATACAAAATTTACAACACACTTATCTACTAAAATTTGTCTTCCACCCGAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:772007/149‑1 (MQ=255)
                                                               cctcgagaaaaaagatGAAAGCGTGCCCTTTGACAGAAAAAAAAAATTTACACCCCCCTTATCTACTGGAATTTGTCTTCCACCCAAAAAACCCGGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:1075075/135‑1 (MQ=255)
                                                               cATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                                       >  2:752146/1‑151 (MQ=255)
                                                               aaTCGGGAAAAATAATGAAAGCGTGCCCTTTGACAAAAAAAAAAATTTTCCACCACCCTTCTCTACTCAATTTTGTTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:1182713/148‑1 (MQ=255)
                                                               aaTCGGGAAAAATAATAAAAGCGTGTCCTTTGACAAAAATAAAAAATTTACAACACACTTATCTCCTAGATTTTGGTTTGCACCCAAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                                       <  2:948242/150‑1 (MQ=255)
                                                                 tgggaaaaaaTAATAAAAGCTTTCCCTTGAACACAAATACAAAATTTTAACCACACTTTTAACCAGAATTTTTTGTTGCACCAGAAAAAACAAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                     <  2:208921/144‑1 (MQ=255)
                                                                  cGGGAGAAATAATGAAAGCGTGTCCCTTGACACAAATACAAAATTTTCAACACACTTATCTCCTCAATTTTGGTTTGCATCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  2:31546/151‑1 (MQ=255)
                                                                  cGGAAGAAATAATGAAAGCGTGTCCCTTGACCCAAATACAAAATTTTCAACACACTAATCTACACGAATTTGTTTTGCACCCTAAAAAACCGGAACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  2:881907/151‑1 (MQ=255)
                                                                   gggAGAAATAATGAAAGCGTGCCCCTTGACAAGAATAAAAAATTTTCAACACCCTTTTCTACCCAATTTTGGTTTGCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  2:539092/150‑1 (MQ=255)
                                                                   ggaaaaaaTAATAAAGGCGTTTCCCTTGACAAAAATAAAAATTTTAAAACACATTTATCTACACAATTTTGTTTTCCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  2:757840/145‑1 (MQ=255)
                                                                    ggAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    >  1:1037809/1‑149 (MQ=255)
                                                                    ggAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTCTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    >  1:1019820/1‑149 (MQ=255)
                                                                           taataaAAGCGTGTCCCTTGACACAAATAAAAAATTTCCAACCCCCTTATCTACTCGATTTTGGTTTGCACCCAAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                           <  1:323529/151‑1 (MQ=255)
                                                                           taatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                            >  1:232297/1‑150 (MQ=255)
                                                                             atGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                          >  1:793398/1‑150 (MQ=255)
                                                                             atGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                          >  1:438909/1‑150 (MQ=255)
                                                                             aaaaaaGCGTGTCCCTTGACACAAATAAAAATTTTTCAACACTTTTATCTCCTCGAATTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc                                                                         <  1:299592/148‑1 (MQ=255)
                                                                                  aGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                                    >  2:445955/1‑151 (MQ=255)
                                                                                  aGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                                    >  2:358548/1‑151 (MQ=255)
                                                                                      tttCCCTGGACACAAAAAAAAAATTTACAACACACTTATCAACACAATTTTGGCTTCCATCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  2:1114064/148‑1 (MQ=255)
                                                                                      ttccccTTGACACAAATAAAAAATTTTCAACACACTTATCTCCTCGATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  1:90004/147‑1 (MQ=255)
                                                                                      ttccccTTGACACAAATAAAAAATTTCCAACACATTTATCTCCTCGATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  1:935411/147‑1 (MQ=255)
                                                                                      tctCCCTTGACAAGAATAAAAAATTTACAACACTCTTATCTACTCAAATTTGGCTTGAATCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  2:569717/148‑1 (MQ=255)
                                                                                          ccTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCGATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                            <  2:43779/151‑1 (MQ=255)
                                                                                          ccTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                             >  2:1134343/1‑150 (MQ=255)
                                                                                                 cacGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                      >  2:611621/1‑151 (MQ=255)
                                                                                                   caaaTAAAAAATTTTCAACACACTTATCCACTCAATTTTGGCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  1:976563/149‑1 (MQ=255)
                                                                                                    gaaaaaaaaaTTTTCAAAAAAACTTTTCTTCCCAATTTTGGCTAGCAACCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                       <  1:1230147/142‑1 (MQ=255)
                                                                                                     aaaaaaaaaTTTCCAACCCACTTATCTAATCAATTTGGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                                  <  2:660524/146‑1 (MQ=255)
                                                                                                      aTACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                                 >  2:627323/1‑151 (MQ=255)
                                                                                                        aCAAATTTTTCAACACCCTTATCTACTAAATTTTGGTTTACATCCGAAAAAACAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAtt                                               <  1:740331/151‑1 (MQ=255)
                                                                                                            atttttAAACACTTTTATCTCCTCAAATTTGGCTTCCCCCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                           <  1:1262325/149‑1 (MQ=255)
                                                                                                            aatatacAACACTCTTATCTACAAAATTTTGGTTTCCCTCCGCAAAAACCCGGTTACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                           <  2:165515/145‑1 (MQ=255)
                                                                                                            aaTTTACAACACCCTTATCTACTCAATTTTGCTTTGCACCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                           <  2:462694/151‑1 (MQ=255)
                                                                                                             aTTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATa                                          >  2:378606/1‑151 (MQ=255)
                                                                                                              tttcCAACACCCTTATCTACTCAATTTTGGCTTGCATCCGAAAAACCAGGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATAGACAATTCCCATGTAGTGATTGCATAGt                                        <  1:1137716/151‑1 (MQ=255)
                                                                                                               tttCAACACCCTAATCTACTCAATTTTGGTTTGCACCCAAAAAAACCGGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                        <  1:627614/151‑1 (MQ=255)
                                                                                                               tttCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                        >  2:757903/1‑151 (MQ=255)
                                                                                                                       ccacTTATCTACTAGAATTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATGGTTGACTTa                                 <  2:580070/150‑1 (MQ=255)
                                                                                                                        cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  2:532064/1‑151 (MQ=255)
                                                                                                                        cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  1:1030003/1‑151 (MQ=255)
                                                                                                                        cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  2:460040/1‑151 (MQ=255)
                                                                                                                           ttttctatccGAATTGGGTTTGAACCCACAAAACCCGGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  2:909675/142‑1 (MQ=255)
                                                                                                                           ttATCTCCTCAAATTTGGCTTGCACCCAAAAAAACCAGCTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  1:90384/151‑1 (MQ=255)
                                                                                                                              tctcctgaaaTTTGCCTTCCCCCCGAAAAACCCAGTACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTAc                          <  1:1023929/143‑1 (MQ=255)
                                                                                                                                    nnanTTTGGCTTCCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                    <  1:512334/149‑1 (MQ=255)
                                                                                                                                     caattttGGTTTCCATCCGAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                    <  1:92407/147‑1 (MQ=255)
                                                                                                                                      aaTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                  >  2:250134/1‑151 (MQ=255)
                                                                                                                                       attttGGCTTGCACCAAAAAAACCCGGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                  <  2:481362/149‑1 (MQ=255)
                                                                                                                                             gtttGCATCCCAAAAACCAGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  1:233771/148‑1 (MQ=255)
                                                                                                                                             gcttccccccGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  1:584951/142‑1 (MQ=255)
                                                                                                                                             gCTTGAATCCGCAAAACCAAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  1:204938/150‑1 (MQ=255)
                                                                                                                                             gCTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  1:478569/150‑1 (MQ=255)
                                                                                                                                              cTTACACCCGCAAAAACCTGCTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg           <  1:625446/151‑1 (MQ=255)
                                                                                                                                                      cGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTaa   >  1:358231/1‑151 (MQ=255)
                                                                                                                                                       gCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAt  >  1:231780/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                         
GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAT  >  NC_000913/3665553‑3665851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: