Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT57.6% 7.1 / 107.2 119S62F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (18/32);  new base T (68/0);  total (87/32)
Fisher's exact test for biased strand distribution p-value = 2.46e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-11

Only 100 of 132 total aligned reads displayed.

CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604196‑4604492
                                                                                                                                                    |                                                                                                                                                         
cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTGTAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                                         >  1:344487/1‑151 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttt                                                                                                                                                       >  2:989072/1‑151 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttt                                                                                                                                                       >  2:900446/1‑151 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCCGTCTAtttttttttt                                                                                                                                                       >  1:1163469/1‑151 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGATTGCGGCGGTCTTtttttctttt                                                                                                                                                       >  2:625466/1‑151 (MQ=255)
   aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttt                                                                                                                                                      >  1:355542/1‑151 (MQ=255)
    tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCGGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                                                                                     <  1:1023196/151‑1 (MQ=255)
               gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCC‑TGAGaa                                                                                                                                        >  2:71598/1‑151 (MQ=255)
                    gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTATTTTTTTTTTTTT‑GCCC‑TGAGAAggggg                                                                                                                                   >  1:586040/1‑147 (MQ=255)
                    gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCC‑TGAGAAGTggg                                                                                                                                   >  1:160138/1‑149 (MQ=255)
                    gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCA‑TGGGAAggggg                                                                                                                                   >  1:65649/1‑147 (MQ=255)
                       tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAgcgc                                                                                                                                <  1:279152/151‑1 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTT‑TCCA‑TGAGAAGGGGgcgccg                                                                                                                              >  1:576031/1‑149 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTT‑TCCA‑TTGGAAAGGAGCGCag                                                                                                                              >  2:609151/1‑151 (MQ=255)
                          taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCag                                                                                                                              <  2:28352/150‑1 (MQ=255)
                            tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCagaga                                                                                                                           <  2:1061656/151‑1 (MQ=255)
                             cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTT‑TCCC‑TTAGAAGGGAgcggcgcgag                                                                                                                          >  2:771794/1‑144 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCA‑TGAGGAGGGAGCGCGGAGgggg                                                                                                                        >  1:1167189/1‑151 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCagggaggg                                                                                                                        >  1:158889/1‑151 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTT‑TCCC‑TGAGGAGGGGGGGGAGAGAggg                                                                                                                        >  2:270031/1‑151 (MQ=255)
                                tATTGAGCGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGCTTTTTTTTTTTTTT‑TCCC‑TTTGAAGGGAACGCGGTGAgggg                                                                                                                       >  2:636831/1‑150 (MQ=255)
                                 aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATTCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                      <  1:1091543/151‑1 (MQ=255)
                                 aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GTCA‑TGAGAAGTGAGCGCAGAGAGGGag                                                                                                                      >  2:402603/1‑150 (MQ=255)
                                 aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                      <  2:967838/151‑1 (MQ=255)
                                     agagTTTAGTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATTCCAGTCGTGAGTCATCTCAGGATGCCATGGCGCGGATCTCGTTTGCGGCGGGCATTTTTTCTTTTACT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGGTTAt                                                                                                                   <  2:1008548/150‑1 (MQ=255)
                                       agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                                  <  2:1025046/149‑1 (MQ=255)
                                       agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑t                                                                                                                <  1:685942/150‑1 (MQ=255)
                                       agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑t                                                                                                                <  2:437836/150‑1 (MQ=255)
                                         tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑Tg                                                                                                             <  2:357331/150‑1 (MQ=255)
                                             ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCA‑TGGGAAGGGAGCGCCGAGAGGGGTTATT‑T‑TGCctga                                                                                                        >  1:379499/1‑151 (MQ=255)
                                                   aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAAc                                                                                                  <  1:270094/151‑1 (MQ=255)
                                                     gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTT‑GCCA‑TGCGAAGGGGGAGGCGAGAGGGGTTATC‑T‑TGTCTGTCTGaa                                                                                                   >  2:462660/1‑148 (MQ=255)
                                                          cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTT‑GCCC‑TGCGGAGAGGGCGGAGAGAGGGATTATT‑T‑TTCCCGCCTGGACTGGG‑GAt                                                                                          >  1:1232699/1‑151 (MQ=255)
                                                                aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCA‑TGGGAAGTGAGCGCAGAGAGGGATTTTT‑T‑TTCCTGACTGAACTGGC‑GGTTAAGtt                                                                                    >  1:482401/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCC‑TGGGAAGTGGGCGCCGAGAGGGATTTTT‑T‑TTCCTGGCTTAAATGGG‑GGTTAAGttt                                                                                   >  1:804886/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCA‑TGGGAAGGGAGGGGAGAGAGGGATTATC‑T‑TTCCTGACTGAAATGGG‑GGTTAAGttt                                                                                   >  1:1133422/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCC‑TGGGAAGTGGGCGGAGGGAGGGGGTTTT‑T‑TTCCTGACTTAACTGGC‑GATTAAAttt                                                                                   >  1:1036886/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATT‑T‑TTCCTTACTGAACTGGC‑GATTTAGatt                                                                                   >  2:807396/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGcc                                                                                                           <  1:745883/128‑1 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGGTTATC‑T‑TTCCTGACCTAAATGGC‑GGTTAAAttt                                                                                   >  2:749093/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCATTTGCGGCGGTCACTTTTTTTTTTTTT‑TCCA‑TGAGGAGTTAGCGCAGAGAGGGATTTTT‑T‑TTCCTGACCGGAATGGA‑GATTTAAttt                                                                                   >  2:968084/1‑151 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTT‑GCCC‑TGAGAAGTGAGCGCCGAGGGGGGTTATC‑T‑TGCCTGACTGAACTGGC‑GATTTAGtttt                                                                                  >  1:450538/1‑149 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGGCCT‑TGAAAAG‑GGGGGCCGGGAGGGGTTTTC‑T‑TGCCTTACCGAACTGGC‑GCTTAAGtttt                                                                                  >  1:1118898/1‑149 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTT‑GCCA‑TGAGAAGGGGGGGCAGAGAGGGATTTTT‑T‑TTCCTGACTGAACCGGC‑GATTAAGGTTc                                                                                  >  1:733398/1‑150 (MQ=255)
                                                                    ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTT‑TCCC‑CGGGAAGTGAGCGCCGAGGGGGGTTATC‑T‑TTCCTTTCTGAACTGGG‑GGTTAAGGTTTcg                                                                                >  1:573611/1‑151 (MQ=255)
                                                                     tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCA‑TGGGAAATTAGCCCGGAGAGGGGTTTTT‑T‑TGCCTTACTTAAATTGC‑GCTTTAGGTTAcgg                                                                               >  2:497657/1‑150 (MQ=255)
                                                                     tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCC‑TGAGAAGTGAGCGCAGAGAGGGGTTATC‑T‑TTCCTGACTGAAATGGC‑GGTTTAGTTTTcg                                                                                >  2:524460/1‑150 (MQ=255)
                                                                     tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTT‑GCCC‑TGAGGAGGGGGCGGAGAGGGGGGTTTTT‑T‑TTCCTGTCTGAACTGGG‑GGTTAAGTTTTcg                                                                                >  1:665198/1‑150 (MQ=255)
                                                                          gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTT‑GCCA‑TGAGGAGGGAGGGCAGAGAGGGCTTATC‑T‑TGCCTGACTGAAATGGC‑GATTTAGTTTTcgcgggt                                                                           >  1:95401/1‑147 (MQ=255)
                                                                          gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCA‑TGAGAAGGGAGGGCAGAGGGGGGTTATT‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTTcgcggaa                                                                           >  1:509439/1‑147 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCA‑TGAGAAGTGAGCGCAGAGAGGGGTTATT‑T‑TTTCTGTCTTAACTGGG‑GATTTAGGTTCCCCGTaac                                                                          >  2:65935/1‑148 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTT‑TCCA‑TGAGAAGGGGGGGCAGGGGGGGGTTTTT‑T‑TTCCTGACTGAAATGGG‑GGTTAAATTTTCGCCGAccccgggt                                                                    >  1:1170822/1‑146 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTT‑TCCC‑TGAGAAGTGGGCGCCGAGAGGGAGTATT‑T‑TGCCTTACTGAACTGGG‑GGTTAAAGTTTCGCGGACCCGGGGt                                                                    >  2:856381/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTT‑TCCC‑TGAGAAGGGAGCGCAGAGGGGGGTTATT‑T‑TTCCTTACTGAAATGGC‑GGTTAAGttttccccggcccggggg                                                                    >  1:1249928/1‑136 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTT‑GCCA‑TGAGAAGGGAGCGCAGGGGGGGGTTATC‑T‑TGCCTTTCTGAACTGGG‑GGTTAAGTTTCCGCGGACCCGGCgg                                                                    >  1:548473/1‑150 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTT‑TCCA‑TGTGAAGGGGGCGCCGAGAGGGATTTTC‑T‑TTTCCTACTGGACTGGC‑GATTTAGGTTTCCCGTACCCCGCGt                                                                    >  1:781576/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCC‑TGAGAAGGGAGGGCAGGGAGGGGTTTTCTT‑TGCTGGACT‑AACTGGG‑GGGTAAGGTTTCGCCGAACCGGCGt                                                                    >  2:474965/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTC‑GTCA‑TGGGAAGTGGGCGGAGGGAGGGGTTATC‑T‑TGTCTGTCTGTACCTGC‑GATTAAGGTTTCGCGTACCCGGCGt                                                                    >  1:531312/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCT‑GCCC‑TTGGAAGGGAGCGCCGGGAGGGGTTATC‑T‑TTCCTGTCTGAACCTGC‑GATTAAGGTTTCGCCGACCCCGCGt                                                                    >  1:1217221/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCT‑GCCC‑TGAGAAGTGAGCGCAGAGAGGGGTTATC‑T‑TGCCCGACTGAACTGGC‑GGTTAAGGTTACGCGTACCCGGCgg                                                                    >  2:286998/1‑150 (MQ=255)
                                                                                tACCTATACCAGGCAGGAGTCATTTCAGGAAGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTTTTTTTCT‑GTCC‑TGGGAAGTGAGCGCAGGGAGGGCGTATT‑T‑TGACTGTCTGTACTTGC‑ACTTAAATTTTCGGGTACACggggg                                                                    >  2:387853/1‑148 (MQ=255)
                                                                                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCC‑TGGGAAGTGGGGGCAGAGAGGGGTTTTC‑T‑TTCCTTACTGAACTGGC‑GATTAAGTTTCCGCGGACCCGGCGtt                                                                   >  1:209649/1‑151 (MQ=255)
                                                                                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCA‑TGCGAAGTGAGGGCAGAGAGGGGTTATC‑T‑TTCCTGACTGAACCGGC‑GATTAAGTTTTCGCGGACCCcgcggt                                                                   >  2:624769/1‑149 (MQ=255)
                                                                                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGGGAGCGCAGAGAGGGGTTATT‑T‑TGCCTGACTGAACTGGC‑GATTAAGGTTCCGCGTACCCGGCGtt                                                                   >  1:797613/1‑151 (MQ=255)
                                                                                  ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTT‑TCCACGGGGAAGGGGGGGC‑GAGAGGGGTTATT‑T‑TTCCTGTCTGAACTTGC‑GGTTTAGGTTTCGCGGAACCgggggtt                                                                  >  1:349198/1‑146 (MQ=255)
                                                                                  ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCA‑TGAGAAGTGAGAGCAGAGAGGGGTTATC‑T‑TGGCTGACTGGACTGGC‑GATTAAGTTTCCGCGGACCCGGCGgtt                                                                  >  2:1130516/1‑151 (MQ=255)
                                                                                  ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTGGCGGCGGACCTTTTTTTTTTTTCT‑GTCC‑TGATTAGGGAGCGCGAAGGGGGATTTTC‑TCTTCCT‑CCTGCACTGGC‑GATTTATTTTCcgtttctcccgcggtt                                                                  >  2:291873/1‑137 (MQ=255)
                                                                                  ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTATGCGGCGGTCTTTTTTTATTTTTTT‑GCCA‑TGAGAAGGGAGCGCCGGGAGGGGTTTTC‑T‑TTCCTTACTTAACTGGCGGTTTAAGTTT‑CGCGCACCCGGCGttt                                                                  >  1:1142639/1‑151 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTGTTTTTTTTTTTTT‑GCCC‑TGAGGAGGGGGCGCCGAGAGGGGATATT‑T‑TTCCTGGCTGAAATGGC‑GATTTAATTTCCCCGGACCCCGCGtcttgt                                                               >  2:1258760/1‑146 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGt                                                               <  2:160107/151‑1 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGCCGCGGATCTCGTTTGCGGCGGCCATTTTTTTTTTTTTT‑TCCT‑TGAGAAGCGAGGGGAGAGGGGGGTTATC‑T‑TCGCTGACTGAATTGGG‑GATTTAAATTCCCCGTACCCTGCGtgttgt                                                               >  1:94382/1‑146 (MQ=255)
                                                                                       aCCAGTCAGGAGTTATTTCTAGATGCCGGGTAGGGAATCTCGTTTGCAGCGGTCATTTTTTCATTTTCT‑GCCA‑AGGGAAGTGAGCAGAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTa                                                             <  2:923110/151‑1 (MQ=255)
                                                                                        ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTT‑GCCA‑TGAGAAGGGGGGGCCGAGGGGGGTTTTT‑T‑TTCCTTACTTAACTGGC‑GATTTAGGTTCCGCGTACCCCGCGGTTTGTTAt                                                            >  1:1026712/1‑151 (MQ=255)
                                                                                          aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCA‑TGAGAAGTGAGCGCAGGGAGGGGTTATC‑T‑TTCCTGACTGAACTGGC‑GGTTAAGTTTTCGCGTACCCGGCGGTTTGTTATTg                                                          >  2:715326/1‑151 (MQ=255)
                                                                                          aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTTTTTTTCT‑GCCC‑TGGGAAGGGAGCGGCGGGGGGGGTTTTC‑T‑TTCCTGACTGGACTTGT‑GGTTTAGTTTTCGCGTACCCCGGGTTTTGTTATTg                                                          >  1:235274/1‑151 (MQ=255)
                                                                                               ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCA‑TGAGAAGTGGGCGCAGAGAGGGGTTATC‑T‑TTCCTGACTGAACTGGC‑GGATTAGTTTTCGCGGACCCCGCGTTTAGGTATTTCGgag                                                     >  2:937285/1‑150 (MQ=255)
                                                                                               ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTT‑GCCC‑TGAGAAGGGAGCGCAGGGGGGGGTTTTC‑T‑TTCCTGACTGAACTGGC‑GGTTAAGTTTCCGCGTACCCGGCGTTTTTTTATTGCgggg                                                     >  1:849200/1‑149 (MQ=255)
                                                                                                    cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTT‑GCCC‑TGAGAAAGGTGGGCGCAGGGGGATTATT‑T‑TGCCTGCATATAATAGC‑GGGTTAAATTCCCCGTAACCGGCGTTTTGTGATTGTGgatgattg                                                >  2:711272/1‑149 (MQ=255)
                                                                                                    cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTT‑GCCC‑TGAGAAGTGAGCGCAGAGGGGGGTTATC‑T‑TGCCTGACTGAACTGGC‑GGTTTAGGTTTCGCGGACCCGGCGTTTTGTTATTTCGgaagatga                                                >  2:179131/1‑151 (MQ=255)
                                                                                                    cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCT‑GCCC‑TGAGAAGTGAGGGCAGAGAGGGGTTATC‑T‑TGCCTGACTGAACTGGC‑GGTTAAGTTTCCCCGGACCCCGCGGTTTGTTATTGCGgatgatgg                                                >  2:392489/1‑150 (MQ=255)
                                                                                                    cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCT‑GCCA‑TGAGAAGGGGGGGCAGGGGGGGGTTTTC‑T‑TTCCTGACTGAACTGGC‑GGTTAAGTTTCCGCGGACCCGGCGTTTTGTTTTTGCGgatggtga                                                >  1:383680/1‑151 (MQ=255)
                                                                                                      tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTT‑TCCA‑TGGGAAGTGAGCGCAGAGAGGGGTTATT‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTTCCCGGACCCGGCGTTTTGTTATTGCGGGTGATGata                                              >  1:243620/1‑151 (MQ=255)
                                                                                                            gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                           <  1:900784/148‑1 (MQ=255)
                                                                                                             atccgaTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                       <  2:1030944/146‑1 (MQ=255)
                                                                                                             aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCT‑GCCA‑TGAGAAGGGAGCGCAGCGAGGGGTTATC‑T‑TGCCTTACTTAACTGGC‑GGTTAAGTTTCCGCGTACCCCGCGGTTTGTTTTTTGGGGTGATGGTATTTAAg                                        >  1:270406/1‑150 (MQ=255)
                                                                                                             aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                        <  2:548253/150‑1 (MQ=255)
                                                                                                             aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                        <  2:1044263/150‑1 (MQ=255)
                                                                                                             aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                       <  1:95824/151‑1 (MQ=255)
                                                                                                              tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                      <  2:665987/151‑1 (MQ=255)
                                                                                                              tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                      <  2:820709/151‑1 (MQ=255)
                                                                                                                    gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTT‑GCCC‑TGAGAAAAGAACGCTGGGAGGGGGTTTT‑T‑TTCCCTACCGTAATGGC‑GGTTTAATTTTCGCGTAACCCGCGTTTTTTTTTTACGGATGATGCTTTTTAAACTCgg                                   >  2:372564/1‑148 (MQ=255)
                                                                                                                          gATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCTTTTAGTTCTTGCGGATTATGATATTGAAGCTCGGCTGATTgg                           <  1:662025/150‑1 (MQ=255)
                                                                                                                          gATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑G‑CA‑TGGGAAGTGAGCGCAGAGGGGGGGTATC‑T‑TTCCTTTTTTAACTTGC‑GATTAAGTTTTCGCGTACCCGGCGCTTAGGTTTTTGGGATGATGAGATTGAAGATCGGGTGATTGGtt                         >  2:526383/1‑151 (MQ=255)
                                                                                                                            tctcGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                        <  1:154501/151‑1 (MQ=255)
                                                                                                                            tctcGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                        <  2:1216712/151‑1 (MQ=255)
                                                                                                                              tcGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                      <  2:477880/151‑1 (MQ=255)
                                                                                                                               cGTTTGCGGCGGTCATTTTTTTTTTTTTT‑TCCC‑TGAGAAGGGAGCGGCGGGAGGGATTATT‑T‑TTCCTGTCTGAAATTGG‑GATTAAGATTTCCCCTACCCGGCGGTTAGTTATTTCGGGTGATGTTATTGTAGCTCGGCTGATTGGGTCttt                     >  2:778579/1‑151 (MQ=255)
                                                                                                                                 tttGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTg                    <  2:266024/150‑1 (MQ=255)
                                                                                                                                                ttttctttttct‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg    <  1:907517/151‑1 (MQ=255)
                                                                                                                                                  ttctttttct‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  <  2:383584/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                         
CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604196‑4604492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑