Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3500CT59.7% ‑5.4 / 106.8 119S64F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (16/32);  new base T (71/0);  total (87/32)
Fisher's exact test for biased strand distribution p-value = 2.24e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-18

Only 100 of 126 total aligned reads displayed.

TAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT  >  NC_000913/4604200‑4604497
                                                                                                                                                       |                                                                                                                                                        
tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCGGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATtttttctttttc                                                                                                                                                          <  1:1023196/151‑1 (MQ=255)
           gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCC‑TGAGaa                                                                                                                                             >  2:71598/1‑151 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CTATTTTTTTTTTTTT‑GCCC‑TGAGAAggggg                                                                                                                                        >  1:586040/1‑147 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑TCCC‑TGGGAAggggg                                                                                                                                        >  1:484704/1‑147 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCC‑TGAGaaaggag                                                                                                                                        >  2:1048736/1‑146 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCC‑TGAGAAGTggg                                                                                                                                        >  1:160138/1‑149 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCA‑TGGGAAggggg                                                                                                                                        >  1:65649/1‑147 (MQ=255)
                   tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAgcgc                                                                                                                                     <  1:279152/151‑1 (MQ=255)
                     gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑TCCA‑TTAGGAGGGGgcggcg                                                                                                                                   >  1:257364/1‑148 (MQ=255)
                     gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑TCCA‑TGAGAAGGGGgcgccg                                                                                                                                   >  1:576031/1‑149 (MQ=255)
                     gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCC‑TGAGAAGGGAGCGCag                                                                                                                                   >  1:1144676/1‑151 (MQ=255)
                     gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCT‑GCCC‑TGAGAAGTGAGCGCag                                                                                                                                   >  2:625964/1‑151 (MQ=255)
                      taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCag                                                                                                                                   <  2:28352/150‑1 (MQ=255)
                      taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGCAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑CCCA‑TTAGAAATgggggccgg                                                                                                                                  >  1:666293/1‑143 (MQ=255)
                         cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTT‑TCCC‑TTAGAAGGGAgcggcgcgag                                                                                                                               >  2:771794/1‑144 (MQ=255)
                          ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCC‑TGAGAAGGGGGCGCAGGGAgg                                                                                                                              >  1:764343/1‑151 (MQ=255)
                           gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCagggaggg                                                                                                                             >  1:158889/1‑151 (MQ=255)
                            tATTGAGCGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑GCTTTTTTTTTTTTTT‑TCCC‑TTTGAAGGGAACGCGGTGAgggg                                                                                                                            >  2:636831/1‑150 (MQ=255)
                             aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATTCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                           <  1:1091543/151‑1 (MQ=255)
                             aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GTCA‑TGAGAAGTGAGCGCAGAGAGGGag                                                                                                                           >  2:402603/1‑150 (MQ=255)
                             aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                           <  2:967838/151‑1 (MQ=255)
                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                                       <  2:1025046/149‑1 (MQ=255)
                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑t                                                                                                                     <  1:939259/150‑1 (MQ=255)
                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑t                                                                                                                     <  2:437836/150‑1 (MQ=255)
                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑t                                                                                                                     <  1:685942/150‑1 (MQ=255)
                                       tATTCAGAAAGTTTACGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGGGTGCGCAGAGAGGGGGTATC‑T‑TGc                                                                                                                 >  2:36686/1‑149 (MQ=255)
                                        aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CATTTTTTCTTTTTTT‑GCCA‑TGTGAAGTGTGCGCAGAGAGGGATTATC‑T‑TGc                                                                                                                 >  2:1217191/1‑148 (MQ=255)
                                         ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCA‑TGGGAAGGGAGCGCCGAGAGGGGTTATT‑T‑TGCctga                                                                                                             >  1:379499/1‑151 (MQ=255)
                                         ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CATTTTTTTTTTTTTT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGAGTATC‑T‑TGCctga                                                                                                             >  2:962884/1‑151 (MQ=255)
                                                      cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑GCCC‑TGCGGAGAGGGCGGAGAGAGGGATTATT‑T‑TTCCCGCCTGGACTGGG‑GAt                                                                                               >  1:1232699/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑TATTTTTTTTTTTTTT‑GCCC‑TGAGAAGTGAGGGCGGAGAGGGGTTATC‑T‑TGCCTGGATGTACTGGC‑GATTTAAttt                                                                                        >  1:640045/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCC‑TGGGAAGTGGGCGCCGAGAGGGATTTTT‑T‑TTCCTGGCTTAAATGGG‑GGTTAAGttt                                                                                        >  1:804886/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCA‑TGGGAAGGGAGGGGAGAGAGGGATTATC‑T‑TTCCTGACTGAAATGGG‑GGTTAAGttt                                                                                        >  1:1133422/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCC‑TGGGAAGTGGGCGGAGGGAGGGGGTTTT‑T‑TTCCTGACTTAACTGGC‑GATTAAAttt                                                                                        >  1:1036886/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTT‑GCCC‑TGAGGAGTGAGGGCCGAGGGGGGGTTTT‑T‑TGCCTGACTGTACTGGC‑GATTAAGgtt                                                                                        >  2:590922/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGGTTATC‑T‑TTCCTGACCTAAATGGC‑GGTTAAAttt                                                                                        >  2:749093/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCATTTGCGGCGGT‑CACTTTTTTTTTTTTT‑TCCA‑TGAGGAGTTAGCGCAGAGAGGGATTTTT‑T‑TTCCTGACCGGAATGGA‑GATTTAAttt                                                                                        >  2:968084/1‑151 (MQ=255)
                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑GCCC‑TGAGAAGTGAGCGCCGAGGGGGGTTATC‑T‑TGCCTGACTGAACTGGC‑GATTTAGtttt                                                                                       >  1:450538/1‑149 (MQ=255)
                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGGCCT‑TGAAAAG‑GGGGGCCGGGAGGGGTTTTC‑T‑TGCCTTACCGAACTGGC‑GCTTAAGtttt                                                                                       >  1:1118898/1‑149 (MQ=255)
                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCC‑TGAGAAAGGGGAGCAGGGAGGGGTTTTC‑T‑TGCCCGACTGAACTGGC‑GGTTAAGgttt                                                                                       >  1:1255472/1‑149 (MQ=255)
                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CATTTTTTTTTTTTTT‑GCCA‑TGAGAAGGGGGGGCAGAGAGGGATTTTT‑T‑TTCCTGACTGAACCGGC‑GATTAAGGTTc                                                                                       >  1:733398/1‑150 (MQ=255)
                                                                 tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCC‑TGAGAAGTGAGCGCAGAGAGGGGTTATC‑T‑TTCCTGACTGAAATGGC‑GGTTTAGTTTTcg                                                                                     >  2:524460/1‑150 (MQ=255)
                                                                 tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CATTTTTTTTTTTTTT‑GCCC‑TGAGGAGGGGGCGGAGAGGGGGGTTTTT‑T‑TTCCTGTCTGAACTGGG‑GGTTAAGTTTTcg                                                                                     >  1:665198/1‑150 (MQ=255)
                                                                      gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CTTTTTTTTTTTTTTT‑GCCA‑TGAGGAGGGAGGGCAGAGAGGGCTTATC‑T‑TGCCTGACTGAAATGGC‑GATTTAGTTTTcgcgggt                                                                                >  1:95401/1‑147 (MQ=255)
                                                                      gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCC‑TGGGAAGTGAGGGCCGAGAGGGGGTATC‑T‑TGCCTGACTGGACTGGC‑GGTTTAGGTTTCGCGGAc                                                                                >  2:1007400/1‑150 (MQ=255)
                                                                      gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCA‑TGAGAAGGGAGGGCAGAGGGGGGTTATT‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTTcgcggaa                                                                                >  1:509439/1‑147 (MQ=255)
                                                                       cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCA‑TGAGAAGTGAGCGCAGAGAGGGGTTATT‑T‑TTTCTGTCTTAACTGGG‑GATTTAGGTTCCCCGTaac                                                                               >  2:65935/1‑148 (MQ=255)
                                                                         ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCC‑TGAGGAGTGAGCGCAGCGAGGGGTTATC‑T‑TGCCTTACTTAACTGGC‑GATTAAGTTTCCGCGTActccg                                                                            >  2:897591/1‑147 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CTTTTTTTTTTTTTTT‑TCCA‑TGAGAAGGGGGGGCAGGGGGGGGTTTTT‑T‑TTCCTGACTGAAATGGG‑GGTTAAATTTTCGCCGAccccgggt                                                                         >  1:1170822/1‑146 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CTTTTTTTTTTTTTTT‑GCCC‑TTAGAAGGGGGGGCCGAGGGGGGTTTTT‑T‑TTCCTGACTTAACTGGG‑GGTTAAGGTTCCGCGTACCCggggg                                                                         >  1:1196871/1‑148 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑TCCC‑TGAGAAGTGGGCGCCGAGAGGGAGTATT‑T‑TGCCTTACTGAACTGGG‑GGTTAAAGTTTCGCGGACCCGGGGt                                                                         >  2:856381/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑GCCA‑TGAGAAGGGAGCGCAGGGGGGGGTTATC‑T‑TGCCTTTCTGAACTGGG‑GGTTAAGTTTCCGCGGACCCGGCgg                                                                         >  1:548473/1‑150 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTCTTTTTTT‑TCCA‑TGTGAAGGGGGCGCCGAGAGGGATTTTC‑T‑TTTCCTACTGGACTGGC‑GATTTAGGTTTCCCGTACCCCGCGt                                                                         >  1:781576/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCC‑TGAGAAGGGAGGGCAGGGAGGGGTTTTCTT‑TGCTGGACT‑AACTGGG‑GGGTAAGGTTTCGCCGAACCGGCGt                                                                         >  2:474965/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCA‑TGAGGAGGGAGCGCAGAGGGGGGATATC‑T‑TGCCTGGCTGAACTGGG‑GGTTAAGTTTTCGCGGACCCCGCGt                                                                         >  1:31845/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTC‑GTCA‑TGGGAAGTGGGCGGAGGGAGGGGTTATC‑T‑TGTCTGTCTGTACCTGC‑GATTAAGGTTTCGCGTACCCGGCGt                                                                         >  1:531312/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCT‑GCCC‑TTGGAAGGGAGCGCCGGGAGGGGTTATC‑T‑TTCCTGTCTGAACCTGC‑GATTAAGGTTTCGCCGACCCCGCGt                                                                         >  1:1217221/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCT‑GCCC‑TGAGAAGTGAGCGCAGAGAGGGGTTATC‑T‑TGCCCGACTGAACTGGC‑GGTTAAGGTTACGCGTACCCGGCgg                                                                         >  2:286998/1‑150 (MQ=255)
                                                                            tACCTATACCAGGCAGGAGTCATTTCAGGAAGCCATGTCGCGGATCTCGTTTGCGGCGGT‑TCTTTTTTTTTTTTCT‑GTCC‑TGGGAAGTGAGCGCAGGGAGGGCGTATT‑T‑TGACTGTCTGTACTTGC‑ACTTAAATTTTCGGGTACACggggg                                                                         >  2:387853/1‑148 (MQ=255)
                                                                             aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCC‑TGGGAAGTGGGGGCAGAGAGGGGTTTTC‑T‑TTCCTTACTGAACTGGC‑GATTAAGTTTCCGCGGACCCGGCGtt                                                                        >  1:209649/1‑151 (MQ=255)
                                                                             aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCA‑TGCGAAGTGAGGGCAGAGAGGGGTTATC‑T‑TTCCTGACTGAACCGGC‑GATTAAGTTTTCGCGGACCCcgcggt                                                                        >  2:624769/1‑149 (MQ=255)
                                                                             aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGGGAGCGCAGAGAGGGGTTATT‑T‑TGCCTGACTGAACTGGC‑GATTAAGGTTCCGCGTACCCGGCGtt                                                                        >  1:797613/1‑151 (MQ=255)
                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑TCCACGGGGAAGGGGGGGC‑GAGAGGGGTTATT‑T‑TTCCTGTCTGAACTTGC‑GGTTTAGGTTTCGCGGAACCgggggtt                                                                       >  1:349198/1‑146 (MQ=255)
                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCA‑TGAGAAGTGAGAGCAGAGAGGGGTTATC‑T‑TGGCTGACTGGACTGGC‑GATTAAGTTTCCGCGGACCCGGCGgtt                                                                       >  2:1130516/1‑151 (MQ=255)
                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTGGCGGCGGA‑CCTTTTTTTTTTTTCT‑GTCC‑TGATTAGGGAGCGCGAAGGGGGATTTTC‑TCTTCCT‑CCTGCACTGGC‑GATTTATTTTCcgtttctcccgcggtt                                                                       >  2:291873/1‑137 (MQ=255)
                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTATGCGGCGGT‑CTTTTTTTATTTTTTT‑GCCA‑TGAGAAGGGAGCGCCGGGAGGGGTTTTC‑T‑TTCCTTACTTAACTGGCGGTTTAAGTTT‑CGCGCACCCGGCGttt                                                                       >  1:1142639/1‑151 (MQ=255)
                                                                                 ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGt                                                                    <  2:160107/151‑1 (MQ=255)
                                                                                   aCCAGTCAGGAGTTATTTCTAGATGCCGGGTAGGGAATCTCGTTTGCAGCGGT‑CATTTTTTCATTTTCT‑GCCA‑AGGGAAGTGAGCAGAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTa                                                                  <  2:923110/151‑1 (MQ=255)
                                                                                    ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTT‑GCCA‑TGAGAAGGGGGGGCCGAGGGGGGTTTTT‑T‑TTCCTTACTTAACTGGC‑GATTTAGGTTCCGCGTACCCCGCGGTTTGTTAt                                                                 >  1:1026712/1‑151 (MQ=255)
                                                                                      aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCA‑TGAGAAGTGAGCGCAGGGAGGGGTTATC‑T‑TTCCTGACTGAACTGGC‑GGTTAAGTTTTCGCGTACCCGGCGGTTTGTTATTg                                                               >  2:715326/1‑151 (MQ=255)
                                                                                      aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CCTTTTTTTTTTTTCT‑GCCC‑TGGGAAGGGAGCGGCGGGGGGGGTTTTC‑T‑TTCCTGACTGGACTTGT‑GGTTTAGTTTTCGCGTACCCCGGGTTTTGTTATTg                                                               >  1:235274/1‑151 (MQ=255)
                                                                                           ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTT‑GCCC‑TGAGAAGGGAGCGCAGGGGGGGGTTTTC‑T‑TTCCTGACTGAACTGGC‑GGTTAAGTTTCCGCGTACCCGGCGTTTTTTTATTGCgggg                                                          >  1:849200/1‑149 (MQ=255)
                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTCTTTTTTT‑GCCC‑TGAGAAAGGTGGGCGCAGGGGGATTATT‑T‑TGCCTGCATATAATAGC‑GGGTTAAATTCCCCGTAACCGGCGTTTTGTGATTGTGgatgattg                                                     >  2:711272/1‑149 (MQ=255)
                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑GCCC‑TGAGAAGTGAGCGCAGAGGGGGGTTATC‑T‑TGCCTGACTGAACTGGC‑GGTTTAGGTTTCGCGGACCCGGCGTTTTGTTATTTCGgaagatga                                                     >  2:179131/1‑151 (MQ=255)
                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCT‑GCCC‑TGAGAAGTGAGGGCAGAGAGGGGTTATC‑T‑TGCCTGACTGAACTGGC‑GGTTAAGTTTCCCCGGACCCCGCGGTTTGTTATTGCGgatgatgg                                                     >  2:392489/1‑150 (MQ=255)
                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCT‑GCCA‑TGAGAAGGGGGGGCAGGGGGGGGTTTTC‑T‑TTCCTGACTGAACTGGC‑GGTTAAGTTTCCGCGGACCCGGCGTTTTGTTTTTGCGgatggtga                                                     >  1:383680/1‑151 (MQ=255)
                                                                                                  tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTT‑TCCA‑TGGGAAGTGAGCGCAGAGAGGGGTTATT‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTTCCCGGACCCGGCGTTTTGTTATTGCGGGTGATGata                                                   >  1:243620/1‑151 (MQ=255)
                                                                                                        gATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                                <  1:900784/148‑1 (MQ=255)
                                                                                                         atccgaTGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                            <  2:1030944/146‑1 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTT‑CCCC‑TCAG‑AGTGAGCGCAGGGCGGGGTTATC‑T‑TTTCTGACTGAACTTGC‑GATTAAGTTTCCGCGTACCCCGCGTTTTGTTATTTCCGATGTTGGTAttttag                                             >  2:486758/1‑146 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCT‑GCCA‑TGAGAAGGGAGCGCAGCGAGGGGTTATC‑T‑TGCCTTACTTAACTGGC‑GGTTAAGTTTCCGCGTACCCCGCGGTTTGTTTTTTGGGGTGATGGTATTTAAg                                             >  1:270406/1‑150 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                             <  2:548253/150‑1 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                             <  2:1044263/150‑1 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                            <  2:199655/151‑1 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                            <  1:95824/151‑1 (MQ=255)
                                                                                                          tGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                           <  2:820709/151‑1 (MQ=255)
                                                                                                                gTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTT‑GCCC‑TGAGAAAAGAACGCTGGGAGGGGGTTTT‑T‑TTCCCTACCGTAATGGC‑GGTTTAATTTTCGCGTAACCCGCGTTTTTTTTTTACGGATGATGCTTTTTAAACTCgg                                        >  2:372564/1‑148 (MQ=255)
                                                                                                                      gATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑G‑CA‑TGGGAAGTGAGCGCAGAGGGGGGGTATC‑T‑TTCCTTTTTTAACTTGC‑GATTAAGTTTTCGCGTACCCGGCGCTTAGGTTTTTGGGATGATGAGATTGAAGATCGGGTGATTGGtt                              >  2:526383/1‑151 (MQ=255)
                                                                                                                        tctcGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                             <  1:154501/151‑1 (MQ=255)
                                                                                                                        tctcGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                             <  2:1216712/151‑1 (MQ=255)
                                                                                                                        tctcGTTTGCGGCGGT‑CATTTTTTCTTTTTCC‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                             <  2:451385/151‑1 (MQ=255)
                                                                                                                          tcGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                           <  2:477880/151‑1 (MQ=255)
                                                                                                                           cGTTTGCGGCGGT‑CATTTTTTTTTTTTTT‑TCCC‑TGAGAAGGGAGCGGCGGGAGGGATTATT‑T‑TTCCTGTCTGAAATTGG‑GATTAAGATTTCCCCTACCCGGCGGTTAGTTATTTCGGGTGATGTTATTGTAGCTCGGCTGATTGGGTCttt                          >  2:778579/1‑151 (MQ=255)
                                                                                                                             tttGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTg                         <  2:266024/150‑1 (MQ=255)
                                                                                                                             tttGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                        <  2:286274/151‑1 (MQ=255)
                                                                                                                                             ttttctttttct‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg         <  1:907517/151‑1 (MQ=255)
                                                                                                                                               ttctttttct‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc       <  2:804563/151‑1 (MQ=255)
                                                                                                                                               ttctttttct‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc       <  2:383584/151‑1 (MQ=255)
                                                                                                                                               ttctttttct‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc       <  2:686963/151‑1 (MQ=255)
                                                                                                                                                    tttct‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGgtgt  <  2:1254387/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                        
TAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCT‑GCCA‑TGAGAAGTGAGCGCAGAGAGGGATTATC‑T‑TGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT  >  NC_000913/4604200‑4604497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑