Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,868,250	A→G	Y448C (TAC→TGC)	yeaG →	protein kinase, endogenous substrate unidentified; autokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,868,250	0	A	G	100.0%	307.3 / NA	84	Y448C (TAC→TGC)	yeaG	protein kinase, endogenous substrate unidentified; autokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/44);  total (40/44)

AAGCCAAGTCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTC  >  NC_000913/1868103‑1868394                                                                                                                                                    |                                                                                                                                                 aaGCCAAGTCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGt                                                                                                                                               <  2:13534/151‑1 (MQ=255)  aGCCAAGTCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGt                                                                                                                                               >  2:590276/1‑150 (MQ=255)    ccAAGTCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCt                                                                                                                                            <  1:627350/151‑1 (MQ=255)      aaGTCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTgg                                                                                                                                          <  1:610184/151‑1 (MQ=255)         tCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc                                                                                                                                       <  1:497794/151‑1 (MQ=255)         tCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc                                                                                                                                       <  1:682729/151‑1 (MQ=255)         tCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc                                                                                                                                       <  2:693012/151‑1 (MQ=255)         tCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAAc                                                                                                                                       <  2:1189282/151‑1 (MQ=255)                 gtaaactCGTGCCTACGGCAGATTCGATGCAGGGATGAACGGACAGTAATCTCGCTCTAAGTTTAAAATCCTCTCCCGCGTGTTCAACTTCGATCAGGAACAACTGGCAACAAACCCGGTCCATCGGTTCTGCGGCCTGGAACAGCAGCtt                                                                                                                               >  2:1081038/7‑151 (MQ=255)                 ggAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAtt                                                                                                                               >  1:52907/1‑151 (MQ=255)                 ggAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAtt                                                                                                                               >  2:627056/1‑151 (MQ=255)                 ggAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAtt                                                                                                                               >  1:592170/1‑151 (MQ=255)                 ggAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAtt                                                                                                                               >  1:500046/1‑151 (MQ=255)                  gAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGAt                                                                                                                                <  2:230124/149‑1 (MQ=255)                   aaTATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTg                                                                                                                              <  1:271455/150‑1 (MQ=255)                     tatCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAg                                                                                                                            >  2:152367/1‑150 (MQ=255)                      ttCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgcg                                                                                                                          <  2:857301/150‑1 (MQ=255)                      atCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAgcg                                                                                                                          >  1:318820/1‑151 (MQ=255)                           gACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAg                                                                                                                      >  1:345712/1‑150 (MQ=255)                             cTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAg                                                                                                                   >  1:1103291/1‑151 (MQ=255)                                     gtgtCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGCTCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcaca                                                                                                           >  2:85094/1‑151 (MQ=255)                                     gtgtCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCTTCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCcaca                                                                                                           >  2:597957/1‑151 (MQ=255)                                       gtCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGCGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAag                                                                                                         >  1:546572/1‑151 (MQ=255)                                         cGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAagag                                                                                                       <  2:867035/151‑1 (MQ=255)                                              aaGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggc                                                                                                  <  2:605574/151‑1 (MQ=255)                                              aaGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggc                                                                                                  <  2:1125109/151‑1 (MQ=255)                                               aGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggca                                                                                                 >  1:953043/1‑151 (MQ=255)                                                gggATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggcag                                                                                                >  1:396805/1‑151 (MQ=255)                                                gggATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggcag                                                                                                >  1:192838/1‑151 (MQ=255)                                                gggATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAgcaggcag                                                                                                >  1:192059/1‑151 (MQ=255)                                                    tGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACg                                                                                            >  1:67701/1‑151 (MQ=255)                                                      agcGGTCTGTCGACGCTTTTGGCGTTTAAGATCCTCTCCCGCGTGTTCACCTTCAATCATGTAGAAGTGGCCGCAAACCCGGTCCATCTGTTCGGCGTCCCTGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAAcac                                                                                           <  2:866376/148‑3 (MQ=255)                                                      aaCGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGc                                                                                           <  2:444651/150‑1 (MQ=255)                                                      aaCGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGc                                                                                           <  1:121839/150‑1 (MQ=255)                                                          gTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTAt                                                                                        >  2:398103/1‑149 (MQ=255)                                                           tCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCt                                                                                      <  1:100509/150‑1 (MQ=255)                                                            cTGTCGACGCGTTTTGCGTTTAAGATCCTCTTCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTgg                                                                                    <  2:427238/151‑1 (MQ=255)                                                            cTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTgg                                                                                    <  2:1008241/151‑1 (MQ=255)                                                               tCGCCGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCAGCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                                                                  <  1:836263/150‑1 (MQ=255)                                                               tCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                                                                  <  1:637540/150‑1 (MQ=255)                                                               tCGACGCGTTGTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCGGAACGCTATCTGGAg                                                                                  <  2:691313/150‑1 (MQ=255)                                                                         ttGCGTTTAAGATCCTCTCCCGCGTTTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                                                                       <  1:1232111/151‑1 (MQ=255)                                                                         ttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                                                                       <  2:1049295/151‑1 (MQ=255)                                                                         ttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                                                                       <  2:840351/151‑1 (MQ=255)                                                                         ttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                                                                       <  2:1240140/151‑1 (MQ=255)                                                                         ttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                                                                       <  2:1247103/151‑1 (MQ=255)                                                                         ttGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                                                                       <  1:550356/151‑1 (MQ=255)                                                                         ttGCGTTTAAGAGCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAgg                                                                       <  2:391744/151‑1 (MQ=255)                                                                              tttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTAt                                                                   >  1:503682/1‑150 (MQ=255)                                                                              tttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATc                                                                  <  1:496877/151‑1 (MQ=255)                                                                               ttAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCt                                                                 <  1:136884/151‑1 (MQ=255)                                                                                tAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTg                                                                <  1:1162523/151‑1 (MQ=255)                                                                                 aaGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGa                                                               >  1:565053/1‑151 (MQ=255)                                                                                    aTCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATcc                                                            <  2:567818/151‑1 (MQ=255)                                                                                    aTCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATc                                                             >  2:927163/1‑150 (MQ=255)                                                                                    aTCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATc                                                             >  1:755165/1‑150 (MQ=255)                                                                                      ccTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAAACCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCg                                                          >  2:736730/1‑151 (MQ=255)                                                                                         ctcCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGaaa                                                       <  1:546112/151‑1 (MQ=255)                                                                                             cgcgTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATg                                                   >  1:385767/1‑151 (MQ=255)                                                                                                  gtTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAg                                              >  2:197616/1‑151 (MQ=255)                                                                                                              tCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCaaa                                  >  1:306216/1‑151 (MQ=255)                                                                                                                aTGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAaga                                >  2:513262/1‑151 (MQ=255)                                                                                                                aTGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAaga                                >  1:218540/1‑151 (MQ=255)                                                                                                                      aaGTGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATc                            <  1:88555/149‑1 (MQ=255)                                                                                                                         ttgcaccgttcctggTCCTTCTGTACTGGGTCCTGGAACAGCGCATTGAGCGCGAGAAGGTTCCACAAGCTCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTAGCTGGTTCCGGAGTATGCCGAGTTTATCGGCAAAGAGACCCAGAc                       <  2:803159/138‑1 (MQ=255)                                                                                                                         tGGCAGCAAACCCGGTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAg                         <  1:846835/149‑1 (MQ=255)                                                                                                                                       gTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGATCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGTTCCAGACGGCCTACCTTGAAt         >  2:1107857/1‑151 (MQ=255)                                                                                                                                       gTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                                                                  <  2:205033/78‑1 (MQ=255)                                                                                                                                       gTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAg                                                                                  >  1:205078/1‑78 (MQ=255)                                                                                                                                       gTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAAt         >  2:792748/1‑151 (MQ=255)                                                                                                                                       gTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAAt         >  2:753039/1‑151 (MQ=255)                                                                                                                                       gTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAAt         >  2:17883/1‑151 (MQ=255)                                                                                                                                       gTCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAAt         >  2:909059/1‑151 (MQ=255)                                                                                                                                        tCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAAt         <  2:976482/150‑1 (MQ=255)                                                                                                                                        tCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAAt         <  2:406866/150‑1 (MQ=255)                                                                                                                                        tCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAAt         <  2:311641/150‑1 (MQ=255)                                                                                                                                        tCCATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATc        <  2:551046/151‑1 (MQ=255)                                                                                                                                         ccATCTGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATcc       <  1:729129/151‑1 (MQ=255)                                                                                                                                         ccATCGGTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCATTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATcc       <  1:1066180/151‑1 (MQ=255)                                                                                                                                               gTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTc  >  2:729395/1‑150 (MQ=255)                                                                                                                                               gTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTc  >  1:712757/1‑150 (MQ=255)                                                                                                                                               gTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTc  >  2:711144/1‑150 (MQ=255)                                                                                                                                               gTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTc  >  1:563025/1‑150 (MQ=255)                                                                                                                                               gTTCTGCGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTc  >  2:970961/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                 AAGCCAAGTCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTCTCCCGCGTGTTCAACTTCGATCATGTAGAAGTGGCAGCAAACCCGGTCCATCTGTTCTACGTCCTGGAACAGCAGATTGAGCGCGAGCAGTTCCCACAAGAGCAGGCAGAACGCTATCTGGAGTTCCTGAAAGGTTATCTGATCCCGAAATATGCCGAGTTTATCGGCAAAGAGATCCAGACGGCCTACCTTGAATCCTATTC  >  NC_000913/1868103‑1868394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑