| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,383,722 | 0 | G | A | 52.0% | 6.7 / 31.4 | 25 | F80F (TTC→TTT) | ycjW | LacI family putative transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (10/2); new base A (0/13); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.02e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-04 | |||||||||||
GAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTT > NC_000913/1383578‑1383857 | gAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGaaaaa > 2:477286/1‑150 (MQ=255) gCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACgg > 1:231353/1‑151 (MQ=255) ataatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTTCAGATCTTCTGCCGAATTGTGCTTCGTTTGTAATATTTCGTCCAAACTTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGTGCATAACGCATTa > 2:582646/1‑150 (MQ=255) aatGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTAccc < 2:119525/151‑1 (MQ=255) ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTACccgccg > 2:301746/1‑151 (MQ=255) ttctttttCTGTTTATTTTTTTTTTCGCTTTTTGGCAGATTTTCTGCCGATTTGGGCGACTTTTGTAATAACACGTCGAACCCTTCTTCTCCAGCTTGGGGGGTGTTGGCTTGAAAAA‑CTTCAGAAAAAACCGGATTACCCGCCGTAGttt < 1:538922/147‑1 (MQ=255) aTCATTTTCTGTTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTCCCGTATTGTGCGACGTTTGTAATATCACGTCAAAAACTTCTTTTTCAGTTTTGGCGGTGTTGGCATGCAAAA‑CTTCAGAAAAAAACGGATTCCCCGCCGTAGttt < 2:647049/151‑1 (MQ=255) aTCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTACCCGCCGTAGttt > 1:318115/1‑151 (MQ=255) ttCTGCTTATTTTTGCTTTCCCTTTTTTGAAGTTCTTCTGCCGGTTTGGGGGATGTTTGTAATACCACGTCGAAACCTTCTTCTTCATCTTTGGCGGTGTTGGCATGCAAAA‑CTGCAAAAAAAACCGTATTCCCCGCCGTAGTTTTGGTCg < 1:517509/151‑1 (MQ=255) tttttttCGTTTTTTGGCAGATCTTCTGCGGTATTGTGCGTTGTTTGTAATATCACGTCAAAACCTTCTTCTTCATCTTGGGCGGTGAGGGCATGAAAAC‑CTTCAGAAAAAAACGAATTACCCGCCGTAGTTTTGGTCGAACGGGTAGaaa < 1:349069/147‑1 (MQ=255) tttcTTTCACTTTTTTGAAGTTTTTCTGCCGATTTGTGGAAGGTTTGAAATACCACGCCAAAACCTTTTTCTTCAGCTTTGGGGGTGATGGCAGACAAAC‑TTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGaaa < 1:66770/148‑1 (MQ=255) ttttGCAGATCTTCTCCCGGATTGGGCGCCGTTTGTAATACCACGTCAAAACTTTCTTCTTCACCTTGGGCGGTGATGGCAGAAAAAC‑CTTAAAAAAAAACCGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATgg < 1:520451/151‑1 (MQ=255) aCGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGcgcgcg > 1:184206/1‑151 (MQ=255) ttGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGc > 2:127031/1‑150 (MQ=255) ttGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGc > 2:376035/1‑150 (MQ=255) gTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAg > 2:102750/1‑151 (MQ=255) ataccaccgaaaaaCCTTATTCTACGGCTTTGGCGGTGATGGCATGAAAAA‑CTTCAGAAAAAAAGGAATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCtt < 2:568137/140‑1 (MQ=255) cGTCGAAACCTTCTTCTTCAGCTTGGGGGGTGAGGGCATGCAAAA‑CTTCAGAAAAAAACGGTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTgg < 1:202457/151‑1 (MQ=255) aCCTTCTTCTCCATTTTGGGCGGTGATGGCAGGAAAAC‑CGTCAAAAAAAAACGAATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATAc < 2:271879/151‑1 (MQ=255) ttcttcttcATTTTTGGCGGTGATGGCATGCAAAG‑CTTCAGAAAAAAACGAATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGt < 1:494635/151‑1 (MQ=255) ttcttcttcAGTTTTGGGGGTGATGGCATACAAAC‑CTTCAGAAAAAAGCGTATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGt < 1:94496/151‑1 (MQ=255) tttcttgAGCTTTGGCGGTGGGGGCA‑GCAAAACCTTCAGAGAAAAACGGTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTaa < 1:411182/150‑1 (MQ=255) tttgggCGGGGATGGCATGAAAAA‑CTTAAAAAAAAAACGAATACCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAAttcttc < 2:231414/144‑1 (MQ=255) agggCATGAAAAC‑CTTCAGAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAac < 2:272144/148‑1 (MQ=255) aTGCAAAA‑CTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACtttt > 2:537805/1‑151 (MQ=255) | GAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAA‑CTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTT > NC_000913/1383578‑1383857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |