Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,115,627	C→T	intergenic (‑137/+35)	mdtG ← / ← lpxL	putative drug efflux system protein/lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,115,627	0	C	T	100.0%	153.6 / NA	38	intergenic (‑137/+35)	mdtG/lpxL	putative drug efflux system protein/lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/27);  total (11/27)

GGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACG  >  NC_000913/1115483‑1115771                                                                                                                                                 |                                                                                                                                                 ggTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCg                                                                                                                                                <  1:614786/147‑1 (MQ=255)  gTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATg                                                                                                                                            >  1:537429/1‑150 (MQ=255)  gTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATg                                                                                                                                            <  2:144276/150‑1 (MQ=255)   tGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGc                                                                                                                                           <  1:525115/150‑1 (MQ=255)     aCATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCAt                                                                                                                                        >  2:568472/1‑151 (MQ=255)           cAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCGAGGGGGCGCAATCCAGAAAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGa                                                                                                                                   <  2:639912/150‑1 (MQ=255)                                    cgGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAg                                                                                                         <  2:328617/151‑1 (MQ=255)                                    cgGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAg                                                                                                         <  1:417877/151‑1 (MQ=255)                                            aTAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAgg                                                                                                 <  2:200806/151‑1 (MQ=255)                                                 aagaaAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGc                                                                                            <  1:314890/151‑1 (MQ=255)                                                     aaaGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTc                                                                                        <  2:97910/151‑1 (MQ=255)                                                         aTAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCg                                                                                      <  2:75497/149‑1 (MQ=255)                                                                  cTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTa                                                                           >  2:217902/1‑151 (MQ=255)                                                                  cTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTa                                                                           >  2:18670/1‑151 (MQ=255)                                                                                 aCTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTaa                                                             >  1:148226/1‑150 (MQ=255)                                                                                   ttCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGGGTGTTTTAAAGCGACGGTGTAGCca                                                          <  2:96927/151‑1 (MQ=255)                                                                                         ggggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACatat                                                     <  1:133093/150‑1 (MQ=255)                                                                                         ggggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACatat                                                     <  1:585202/150‑1 (MQ=255)                                                                                           ggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATAc                                                   <  1:210478/150‑1 (MQ=255)                                                                                           ggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATAc                                                   <  2:499142/150‑1 (MQ=255)                                                                                           ggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATAc                                                   <  1:314292/150‑1 (MQ=255)                                                                                           ggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATAc                                                   <  1:548511/150‑1 (MQ=255)                                                                                           ggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATAc                                                   <  1:573019/150‑1 (MQ=255)                                                                                           ggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATAc                                                   <  1:80034/150‑1 (MQ=255)                                                                                           ggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATAc                                                   <  1:629713/150‑1 (MQ=255)                                                                                           ggCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATAc                                                   <  1:619856/150‑1 (MQ=255)                                                                                            gcgcAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACt                                                  <  2:537906/150‑1 (MQ=255)                                                                                             cgcAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGc                                                <  1:647167/151‑1 (MQ=255)                                                                                             cgcAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTATGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTg                                                 <  2:576840/150‑1 (MQ=255)                                                                                               cAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGctc                                              <  1:308985/151‑1 (MQ=255)                                                                                                 aTCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGct                                               >  1:384449/1‑148 (MQ=255)                                                                                                 aTCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGct                                               >  1:641356/1‑148 (MQ=255)                                                                                                       agagCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGCGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTg                                         <  1:636956/148‑1 (MQ=255)                                                                                                                             ggggATTTGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGAc                   <  1:67830/148‑1 (MQ=255)                                                                                                                                   ttGCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGtt          >  1:204533/1‑151 (MQ=255)                                                                                                                                     gCTGTGGTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCa        >  2:530532/1‑151 (MQ=255)                                                                                                                                          ggTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCa    >  2:232248/1‑150 (MQ=255)                                                                                                                                           gTAATGTCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCAcg  >  1:17256/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACG  >  NC_000913/1115483‑1115771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑