Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,338,928	C→A	L107F (TTG→TTT)	mlaE ←	ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,338,928	0	C	A	100.0%	201.5 / NA	50	L107F (TTG→TTT)	mlaE	ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/25);  total (25/25)

GAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACAA  >  NC_000913/3338788‑3339073                                                                                                                                             |                                                                                                                                                  gAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg                                                                                                                                         >  1:608316/1‑151 (MQ=255)       aCCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTACCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacca                                                                                                                                   >  2:423701/1‑151 (MQ=255)              cccagAAACGGGGAGAAGTACCCCGACGCCGCGGATCCCCCGCCAGCATCTCCATCCTGGAGAGTTGCCCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCCGCACGCCCGGCAAAAAACAACGCGGCAACCCCCgg                                                                                                                               <  2:311959/148‑1 (MQ=255)                   aaaCGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCTGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCCCCGGCCCCAGtt                                                                                                                       <  2:381345/151‑1 (MQ=255)                   aaaCGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                                                                       <  2:409322/151‑1 (MQ=255)                        gggAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACg                                                                                                                   >  1:486213/1‑150 (MQ=255)                          gagaAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCa                                                                                                                 <  2:602914/150‑1 (MQ=255)                            gaAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTa                                                                                                              >  1:585922/1‑151 (MQ=255)                            gaAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTa                                                                                                              >  2:22792/1‑151 (MQ=255)                            gaAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTa                                                                                                              >  2:405631/1‑151 (MQ=255)                             aaaTAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                                                                             <  2:58380/151‑1 (MQ=255)                             aaaTAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                                                                             >  2:135206/1‑151 (MQ=255)                                tAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGa                                                                                                          <  2:127232/151‑1 (MQ=255)                                  aCCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa                                                                                                        >  1:287323/1‑151 (MQ=255)                                               gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                                                                           >  1:134296/1‑151 (MQ=255)                                                   cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                                                                       <  1:274554/151‑1 (MQ=255)                                                   cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                                                                       <  2:474341/151‑1 (MQ=255)                                                   cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                                                                       <  2:616066/151‑1 (MQ=255)                                                       gCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGa                                                                                    >  1:623220/1‑150 (MQ=255)                                                            catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGtt                                                                              >  2:402019/1‑151 (MQ=255)                                                              tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                                                                             >  1:586078/1‑150 (MQ=255)                                                              tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                                                                             >  1:619847/1‑150 (MQ=255)                                                                   ntACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCa                                                                        >  2:193028/2‑150 (MQ=255)                                                                   aTACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCa                                                                        >  2:413358/1‑150 (MQ=255)                                                                   aTACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGCTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGCCAACCACCGGCCCCAGTTCACGCCGTAGCGATAAAGCCACCAGCATACCCAGACTGGTTTCCGc                                                                         >  2:134129/1‑149 (MQ=255)                                                                      cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtat                                                                    >  2:229195/1‑151 (MQ=255)                                                                      cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtat                                                                    >  1:342013/1‑151 (MQ=255)                                                                          agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCCCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg                                                                 <  2:635162/150‑1 (MQ=255)                                                                            agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg                                                               <  1:583594/150‑1 (MQ=255)                                                                            agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg                                                               <  1:400132/150‑1 (MQ=255)                                                                             gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGt                                                             >  2:424811/1‑151 (MQ=255)                                                                              ttGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATCCCCAGACTGGTTTCCGCACTATAAGTGGt                                                             <  1:228978/150‑1 (MQ=255)                                                                                gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGCAGCGATAACGCCACCAGCATACCTAGACTGGTTTCCGCACTATAAGTGGTCAg                                                          >  1:265181/1‑151 (MQ=255)                                                                                     gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGACAGAACCa                                                     >  2:463944/1‑151 (MQ=255)                                                                                         cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAt                                                  <  1:253043/150‑1 (MQ=255)                                                                                           cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc                                               <  1:242071/151‑1 (MQ=255)                                                                                           cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc                                               <  2:458484/151‑1 (MQ=255)                                                                                             cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc                                               >  1:288760/1‑149 (MQ=255)                                                                                             cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCt                                             <  1:262274/151‑1 (MQ=255)                                                                                             cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCt                                             <  1:263138/151‑1 (MQ=255)                                                                                             cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGGGGTCAGAACCAGATAAc                                               >  2:217249/1‑149 (MQ=255)                                                                                                           cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac                               <  2:558421/151‑1 (MQ=255)                                                                                                           cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac                               <  2:397755/151‑1 (MQ=255)                                                                                                            tGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACAcc                              <  1:381318/151‑1 (MQ=255)                                                                                                               ggTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCAtt                           >  2:564366/1‑151 (MQ=255)                                                                                                                 ttAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTcc                         <  2:120975/151‑1 (MQ=255)                                                                                                                     cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGa                       <  1:469510/149‑1 (MQ=255)                                                                                                                                  cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGaaa        <  2:52541/151‑1 (MQ=255)                                                                                                                                    cccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAAc       <  1:424663/150‑1 (MQ=255)                                                                                                                                        gCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACaa  >  1:285812/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                  GAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACAA  >  NC_000913/3338788‑3339073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑