Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,282	0	T	G	48.6%	84.8 / 20.2	73	V526G (GTT→GGT)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (13/24);  new base G (35/0);  total (49/24)
Fisher's exact test for biased strand distribution p-value = 5.01e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCG  >  NC_000913/354133‑354429                                                                                                                                                      |                                                                                                                                                      cgttGACTGGTGCATCCGTGACGCTGACTGTTATCTCTTTATTCTCGGGCGCTCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGGGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGtt                                                                                                                                                      <  2:1523409/149‑1 (MQ=255)    tGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTggg                                                                                                                                                   >  1:448667/1‑151 (MQ=255)          gggCATCCGTGACGCTGACGGTTATCACATTATTCTCGGGCGCACTAACGGTGTAATTAACGCTGCCGGGCATCGGCTGGGGAAGCGCGAGCTTGAAGAGGTTATCGCCAGCCAGCCGGGCGTTGCCCATGTGGCGGGGGATggggggccg                                                                                                                                             >  2:1451796/1‑146 (MQ=255)            gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg                                                                                                                                            <  2:657147/150‑1 (MQ=255)             cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                           <  1:344101/150‑1 (MQ=255)             cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                           <  1:124184/150‑1 (MQ=255)              aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTgggggggaaaagg                                                                                                                                         >  2:244841/1‑142 (MQ=255)               tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGgggtggggtgggagagg                                                                                                                                         >  2:741388/1‑143 (MQ=255)               tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATgg                                                                                                                                        >  2:1086348/1‑150 (MQ=255)                        cTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAgg                                                                                                                              <  1:679833/151‑1 (MQ=255)                            cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGGT‑GGAAggggcg                                                                                                                          >  2:395953/1‑148 (MQ=255)                            cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGGAAAgggag                                                                                                                           >  1:1015161/1‑149 (MQ=255)                              gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGGGCG‑GAAAGGGCAggg                                                                                                                        >  1:421449/1‑150 (MQ=255)                              gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCG‑GGAAGGGGAggg                                                                                                                        >  1:906736/1‑150 (MQ=255)                                tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtgg                                                                                                                      <  2:933819/151‑1 (MQ=255)                                    aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGGGGT‑GGAAGGGGAggtgggggg                                                                                                                  >  2:308798/1‑150 (MQ=255)                                     cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCT‑GGAAGGGGCggtggggggg                                                                                                                 >  2:1582117/1‑149 (MQ=255)                                     cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGAAGCGCT‑GGAAGGGGAgggggcgggg                                                                                                                 >  2:1516370/1‑149 (MQ=255)                                         aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAgggggggggggggg                                                                                                             >  1:481796/1‑148 (MQ=255)                                         aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCT‑GAAAGGGGAgggggcgggggggg                                                                                                             >  1:1348243/1‑148 (MQ=255)                                            ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGa                                                                                                                                           >  1:527090/1‑119 (MQ=255)                                            ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGGATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                           <  2:526897/119‑1 (MQ=255)                                              cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTc                                                                                                        <  1:1526366/151‑1 (MQ=255)                                                ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGGGGTTGTCAt                                                                                                      >  1:1470625/1‑151 (MQ=255)                                                   gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAAATGCGCT‑GAAAGGGGAGGTGGCGGGGGCGGTTGTCATTc                                                                                                    >  2:1542146/1‑150 (MQ=255)                                                    cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGGTGGAAAGGGCGGGGGGGGGGGGGGttgtgatttcg                                                                                                   >  2:928231/1‑142 (MQ=255)                                                        gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCCTCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCT‑GACAGGGGCGGTGGCGGGGGGGTTTTTTCTTCCGgaaa                                                                                              >  2:888046/1‑150 (MQ=255)                                                        gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGgaaa                                                                                              >  2:224557/1‑150 (MQ=255)                                                        gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTTTCATTTCGAAag                                                                                              >  1:1114771/1‑151 (MQ=255)                                                         aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                              <  1:832980/150‑1 (MQ=255)                                                         aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCACTCCGAAag                                                                                              <  1:259470/150‑1 (MQ=255)                                                         aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCT‑GGAAGGGGCGGGGGCGGGGGGGGTTGTCCTTCCGAAagg                                                                                             >  2:216517/1‑150 (MQ=255)                                                            aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGCGTTTGTTCTTTCGAAagagag                                                                                          >  2:1371500/1‑151 (MQ=255)                                                            aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCAATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagagag                                                                                          <  2:1269238/151‑1 (MQ=255)                                                             tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGGAAAGGGC‑GGGGGGGGTGGGGGTTGTCATTCCGGAagagag                                                                                          >  1:1436010/1‑150 (MQ=255)                                                              gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCT‑GGAAGGGGCGGTGGGGGGGGGGGTTGTTATTCCGGAAGAGAGC‑g                                                                                       >  2:1555386/1‑151 (MQ=255)                                                              gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCTTTCCGAAagaggggg                                                                                        >  1:1409375/1‑147 (MQ=255)                                                              gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCC‑GGAAAGGGAGGGGGGGGTGGGGGTTGTCATTCCGGAagagaggg                                                                                        >  2:942246/1‑149 (MQ=255)                                                              gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCT‑GGAAAGGCAGGGGGGGGGGGGGGTTGTCCTTCCGGAagagaggg                                                                                        >  1:724118/1‑149 (MQ=255)                                                              gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCC‑GGAAGGGGAGGGGGCGGGGGCGGTTGTCATTTCGAAAGAGAGC‑g                                                                                       >  2:1343700/1‑151 (MQ=255)                                                              gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGGGAAAGAAGCGCT‑GAAAGGGCAGGGGGGGGGGGGGGTTGTTCTTCCGAAAGAGAGC‑g                                                                                       >  1:602727/1‑151 (MQ=255)                                                                aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GAc                                                                                     <  1:718047/151‑1 (MQ=255)                                                                aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGGC‑GGAAGGGGGGGGGGGGGGGGGGGTTGGCATTCCCGAagagagggga                                                                                      >  2:777250/1‑147 (MQ=255)                                                                  ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAg                                                                                   <  1:167322/151‑1 (MQ=255)                                                                     aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCt                                                                                <  1:1584156/151‑1 (MQ=255)                                                                        ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTgg                                                                              <  1:1412996/150‑1 (MQ=255)                                                                           ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAg                                                                           <  2:1475234/150‑1 (MQ=255)                                                                           ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAg                                                                           <  1:1061476/150‑1 (MQ=255)                                                                           ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAg                                                                           <  2:619296/150‑1 (MQ=255)                                                                              ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGGGGAAGATGGGCC‑GGAAGGGGAGGGGGGGGGGGGGGTTGGCATTCCGAAAGAGAGG‑GGCAGGCTGGGAGATCg                                                                       >  1:571000/1‑151 (MQ=255)                                                                                  tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGAt                                                                   <  1:83872/151‑1 (MQ=255)                                                                                    ggCTGGGGACGCGCGAGTTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGGGAtgt                                                                 >  1:221181/1‑151 (MQ=255)                                                                                    ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCT‑GGAAAGGCAGGGGGGGGGGGCGGTTGTCCTTCCCTAAGAGAGCGGACA‑TCTGGGAGAGCGCGAtgt                                                                 >  2:620289/1‑151 (MQ=255)                                                                                     gCTGGGGGCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGAtgt                                                                 <  2:281370/150‑1 (MQ=255)                                                                                     gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCCCT‑GAAAGGGGCGGTGGCGGCGGCGTTTGTCATTTCCAAAGAGAGG‑GGCAGTCTGGAAGATCGGGAtgtg                                                                >  1:453235/1‑151 (MQ=255)                                                                                     gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCT‑GAAAAGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAAAGAGG‑GAAAGGCTGGAAGATCGGGagggg                                                                >  1:351404/1‑147 (MQ=255)                                                                                                   agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAg                                                                           <  1:732659/126‑1 (MQ=255)                                                                                                   agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGGGCT‑GAAAGGGGAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGGGATGTGGCGCCCTCGCAaga                                                  >  1:1245472/1‑151 (MQ=255)                                                                                                     attggtgagggTATTTCCGGGCATCCGGGCGTTGCCGAAGTGGCGTTGGTTGGGGTGAAAGATGCGCT‑GAATGGGCAGGTGGCGGTGGCGTTTATCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCTCACTCGCAagata                                                <  2:306943/145‑3 (MQ=255)                                                                                                     aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGGGCT‑GAAAGGGGAGGGGGGGGGGGGGTTTGTTATTTCGAAAGAGAGG‑GACAGTCTGGAAGATCGTGGTGTGGGGCCCTCGCAAggga                                                >  1:467863/1‑151 (MQ=255)                                                                                                     aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAAAGAGC‑GACAGTCTGGGAGATCGTGATGTGGGGCACCCGCAAgaga                                                >  2:1502212/1‑151 (MQ=255)                                                                                                     aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGGGCT‑GAAAGGGGAGGGGGGGGGGGCGTTTGTCCTTCCGAAAGAAAGC‑GGCAGGCTGGGAGAACGTGATGTGGCGCACCCCCAAgaga                                                >  1:1565347/1‑151 (MQ=255)                                                                                                       tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                              <  2:18908/151‑1 (MQ=255)                                                                                                             gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGCGGTTGTCATTTCGAAAAAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                        >  2:505123/1‑151 (MQ=255)                                                                                                             gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGCT‑GGAAGGGGCGGGGGGGGGGGGGGTTGTTATTCCGAAAGAGAGG‑GGAAGTCTGGAAGACCGGGATGGGGCGCACCCCCAAGAGAAGGCGAtt                                        >  2:814526/1‑151 (MQ=255)                                                                                                             gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGGtt                                        >  1:845947/1‑151 (MQ=255)                                                                                                             gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGG‑GACAGTCTGGAAGATCGGGGTGTGGGGCACTCGCAAGAGAAGGGGAtt                                        >  1:426868/1‑151 (MQ=255)                                                                                                               gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGGTGGGGGGCACTCGCAAGAGAAGGGGATTAt                                      >  1:565640/1‑151 (MQ=255)                                                                                                                tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                      <  1:208176/150‑1 (MQ=255)                                                                                                                 aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                    <  1:1340549/151‑1 (MQ=255)                                                                                                                 aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCT‑GAAAGGGCAGGGGGGGGGGGCGGTTTTCCTTCCGAAAGAGAGG‑GGCAGTTTGGGAGATCGTGATGTGGGGCACTCGCAAGAGAAGGGGGTTATgg                                    >  1:1568195/1‑151 (MQ=255)                                                                                                                 aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GGAAGGGCAGGGGGGGGGGGCGGTTTTCATTCCGGAAGAGAGC‑GACAGTCTGGAAGAACGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                    >  2:577455/1‑151 (MQ=255)                                                                                                                        gCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGG‑GACAGGCTGGGAGATCGGGATGTGGCGCCCCCGCCAGAGAAGGCGATTATGGCGCtggg                             >  1:1037263/1‑150 (MQ=255)                                                                                                                          cATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGGGATGTGGCGCACCCGCCAGAGAAGGGGGTTTTGGCGCtggggg                           >  1:1159083/1‑151 (MQ=255)                                                                                                                                            gTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa           >  2:1464913/1‑149 (MQ=255)                                                                                                                                                  gtggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc    <  1:593425/150‑1 (MQ=255)                                                                                                                                                    ggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg  >  1:1380493/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CTTTGACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCG  >  NC_000913/354133‑354429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑