Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	50.0%	51.5 / 83.6	82	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (34/7);  new base A (0/41);  total (34/48)
Fisher's exact test for biased strand distribution p-value = 3.47e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGA  >  NC_000913/29349‑29643                                                                                                                                                    |                                                                                                                                                    aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaa                                                                                                                                                  >  1:404753/1‑151 (MQ=255)     tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                              <  1:371453/151‑1 (MQ=255)     tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                              <  2:501386/151‑1 (MQ=255)        aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                           <  2:205730/151‑1 (MQ=255)                  tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                                 >  2:241716/1‑151 (MQ=255)                          aCTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                         >  2:1097913/1‑151 (MQ=255)                           cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTa                                                                                                                        >  2:1124373/1‑151 (MQ=255)                            ttttttttGCTGTTTTTTTTTTAATGCATTTTTTTTGCTGCGCAAGCGTTTCCCAAAACAGTTAAGATAATTTTTTTGTCGTTTAAGGCTTAAAAAACATGCATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                                       <  2:512256/151‑1 (MQ=255)                            ttttttCTTTTCTTTTTTTTTTCATGATATCTTCTTTCTGCGAAAGCTTTTCACATAACGGTTGATATTTTTTTTTTCTGTTTTAGTCCTTATAAAAGATACTTGACCCAAAAAATAAAAAAAAAAACTCCGCCATTAAGTTGACTTTTAg                                                                                                                       <  2:747584/151‑1 (MQ=255)                            ttttttCTCCTTTTTTTTATTTAATGAATTCTTTTTGCGGCACAAGCTTTTACAAAACCAGGTTATAAAATTTTTTTGTCTTTTATTCCTTGAAAACCAGGCATGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                                       <  2:1052409/151‑1 (MQ=255)                                       tttttttATTTAATGCATTTTTTTGACTGCGCAAGCTTTTCCCAAAACAGTTTAGATGATTTTTTTGTCGCTTAATCCCTGAAAAACATGCTTGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                            <  2:50224/149‑1 (MQ=255)                                         tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                           >  2:459229/1‑150 (MQ=255)                                           ttttttTCATACATTCTTCTTGCTGCGCAAGCTTTTACAAAAACAGGTAAGATTATTTTTTTGTCGTTAAATGCCTAAAAAACTGGCGTGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                        <  2:125955/151‑1 (MQ=255)                                           tttATTTCATGAAATCTTTTTGCTGCACAAGCTTTTTCAAAAACAGGTTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCAGGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                        <  1:1110300/151‑1 (MQ=255)                                            ttATTTCATACATTCTTCTTCCTGCGCAGGTTTTTCCCAAAAGAGTTAAGATGATTTTTTTTTCGCTTATGGCCGGAAAAACATGAATGAGCCACAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                       <  2:306273/151‑1 (MQ=255)                                             tttttCATGCATTCTTCTTGCTGCGCAAGCTTTTCCAAGAACAGGTTAGATATTTTTTTTTTCGTTTATTGCCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGaa                                                                                                      <  2:1460072/149‑1 (MQ=255)                                             tATTTCATGCATTTTTTTTGCTGCGAAAGCGTTTTCCAGAACGGGTTAGATGATTTTTTTGTCGTTTAATGCCTAAAAAACATGATTGGCCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGaa                                                                                                      <  2:1426896/151‑1 (MQ=255)                                              ttttCATCCATTCTTCTGGCTGCAAAAGTTTTTCCAAGAACAGTTAAAATGATTTTTTTGCCGTTAAATGCTTAAAAAACTGCCAGGACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                     <  2:942092/150‑1 (MQ=255)                                              aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                     >  1:1573557/1‑151 (MQ=255)                                               tttCAGGCATTCTTCTTGCGGCACAAGCTTTTCCAAGAACAGTTAAGATGATTTTTTTGTCGTTTAATCCTTAAAAAACATGCATGCCCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                                    <  2:1020344/151‑1 (MQ=255)                                                ttCATCCATTTTTTTTGCGGCGAAAGCGTTTCCAAAAACGGTTTAGATGATCTTTTTGTCGTTTAATGCCTGAAAAACATGCATGGCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                                   <  1:1576140/151‑1 (MQ=255)                                                        aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCTCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                           >  2:1131275/1‑151 (MQ=255)                                                        aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                           >  2:1014937/1‑151 (MQ=255)                                                        aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                           >  1:552124/1‑151 (MQ=255)                                                        aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                           >  2:1210996/1‑151 (MQ=255)                                                                 ctgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                                  >  1:522592/1‑151 (MQ=255)                                                                    cgaaaGCTTTTTCAAAAAAAGTTAAAATTATTTTTTTGTCGTTTAATGCTTGAAAAACATGATTGAGCAAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                               <  1:513090/148‑1 (MQ=255)                                                                      aaaGCTTTTCCCAAAACGGGTAAAATGATTTTTTTGTCTCTTAATGCCTAAAAAACATGCATGGGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                             <  1:1486789/150‑1 (MQ=255)                                                                             ttcccAGAACAGGTAAGATGATTTTTTTGTCGTTTAGTCCCTGAAAAACATGAAGGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                      <  2:1190046/148‑1 (MQ=255)                                                                             ttccaaaaaaaGGTTAAATTATTTTTTTGCCGTTAATTGCCTAAAAAACATGAATGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                      <  2:1041785/141‑1 (MQ=255)                                                                              ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  2:20572/1‑151 (MQ=255)                                                                              ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  2:750901/1‑151 (MQ=255)                                                                              ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  2:951685/1‑151 (MQ=255)                                                                              ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  1:1330647/1‑151 (MQ=255)                                                                              ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  1:1061390/1‑151 (MQ=255)                                                                              ttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                     >  1:1482323/1‑151 (MQ=255)                                                                                caaaaaCAGGTAAGATATTTTTTTTGCCCATTAATGCTTTAAAAACTTGCAGGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                   <  2:1441931/147‑1 (MQ=255)                                                                                 aaagaCAGTTTAGATTATTTTTTTGTCGCTAAATGCCTTAAAAAAATGCATGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                   <  1:971560/146‑1 (MQ=255)                                                                                   gAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                                >  1:914128/1‑151 (MQ=255)                                                                                    aacggtttAGATAATTTTTTTTCCTATAAATCCGTGAAAAACTTGCATGACCCACAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                                 <  2:1066870/143‑1 (MQ=255)                                                                                     aCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                   >  2:971071/1‑146 (MQ=255)                                                                                        ggTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGTGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGCTTGCCAGAAAATCGTCGGCGAGCAGATTTACAtt                                                           >  2:691423/1‑151 (MQ=255)                                                                                        ggTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                           >  2:336858/1‑151 (MQ=255)                                                                                         gTTAGATGTTTTTTTTGTCCTTTAAGGCTTGAAAAACATCCATAACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                            <  2:63293/149‑1 (MQ=255)                                                                                             gatgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                      >  1:324806/1‑151 (MQ=255)                                                                                                 tttttttttTCCTTTAATGCCTTAAAAACATGAATGGCCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                   <  1:520717/147‑1 (MQ=255)                                                                                                 ttttttttGTCTTTTAATCCTTGAAAAAAATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  <  1:251401/148‑1 (MQ=255)                                                                                                 ttttttttGTCGTTTAATGCCTAAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  <  1:1144521/148‑1 (MQ=255)                                                                                                 ttttttttGTCGCTTAAGGCCTGAAAAACAGGCATAACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  <  1:575120/148‑1 (MQ=255)                                                                                                 ttttttttGTCCTTTAATGCAGAAAAAACATGAATGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  <  1:1488806/148‑1 (MQ=255)                                                                                                 atttttttGTCGCTTAATGCCTGAAAAACATGAATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  <  1:1264905/148‑1 (MQ=255)                                                                                                 atCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGACTTACGt                                                  >  1:968227/1‑151 (MQ=255)                                                                                                  tCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  >  1:488588/1‑150 (MQ=255)                                                                                                  tCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  >  2:1027585/1‑150 (MQ=255)                                                                                                     ttttGTCCTTTAAGGCTTAAAAAACAGGCAGGACCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                              <  1:244453/151‑1 (MQ=255)                                                                                                       ttgtgaattaagcccTGTAAAACGGGTTTGGGCCAAAAAAAAATATAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                            <  1:576170/138‑1 (MQ=255)                                                                                                       ttGTCGTTTAATGCCTGAAAAACATGCAGGCCCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                            <  1:516054/151‑1 (MQ=255)                                                                                                         ttcctttAATGCTTGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                          <  1:323970/146‑1 (MQ=255)                                                                                                          tcgttaactgccgaaaaaaaaTGCGTGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                          <  2:982194/135‑1 (MQ=255)                                                                                                          tCGCTTAATGCGTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                         <  2:693601/151‑1 (MQ=255)                                                                                                             cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                      >  1:1544019/1‑151 (MQ=255)                                                                                                             cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                      >  2:421885/1‑151 (MQ=255)                                                                                                               aatgcccTGAAAAACATGAATGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                    <  2:146533/146‑1 (MQ=255)                                                                                                                  tGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                 >  1:270109/1‑151 (MQ=255)                                                                                                                    cttgaaaaaCATGCAGGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                                <  1:927709/145‑1 (MQ=255)                                                                                                                    ccTGAAAAACAGGCAGGAGCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                                <  1:1307191/150‑1 (MQ=255)                                                                                                                     cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                              >  2:473759/1‑151 (MQ=255)                                                                                                                          aaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                         >  2:1299819/1‑151 (MQ=255)                                                                                                                              atgcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                     >  1:437104/1‑151 (MQ=255)                                                                                                                                gcaggAGCAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                     <  1:652510/149‑1 (MQ=255)                                                                                                                                 caggaccaacaAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                   <  2:205960/142‑1 (MQ=255)                                                                                                                                       ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat            >  1:440582/1‑151 (MQ=255)                                                                                                                                       ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat            >  1:542716/1‑151 (MQ=255)                                                                                                                                         aaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat          <  1:1574331/149‑1 (MQ=255)                                                                                                                                           aaaaaaaaaaaaaaACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc        <  2:784400/151‑1 (MQ=255)                                                                                                                                           aaaaaaATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc        <  1:1445462/151‑1 (MQ=255)                                                                                                                                            aaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt         >  2:802447/1‑149 (MQ=255)                                                                                                                                            aaaTAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct       <  2:1103241/151‑1 (MQ=255)                                                                                                                                              ataataAAAAAAGCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct     <  2:146044/151‑1 (MQ=255)                                                                                                                                              aaaataTAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct     <  1:241779/149‑1 (MQ=255)                                                                                                                                                aataAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg   <  1:1082765/151‑1 (MQ=255)                                                                                                                                                aataAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg   <  1:1270096/151‑1 (MQ=255)                                                                                                                                                aataAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg   <  1:288095/151‑1 (MQ=255)                                                                                                                                                 atatAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGa  >  2:1322654/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    AATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGA  >  NC_000913/29349‑29643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑