Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913345,3280CA46.4% 125.5 / 73.8 110intergenic (+337/‑76)yahL/yahMuncharacterized protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (50/9);  new base A (0/51);  total (50/60)
Fisher's exact test for biased strand distribution p-value = 3.30e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTG  >  NC_000913/345178‑345475
                                                                                                                                                         |                                                                                                                                                   
taAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATc                                                                                                                                                     <  1:267433/151‑1 (MQ=255)
   aaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCaaa                                                                                                                                                  >  1:225099/1‑151 (MQ=255)
    aaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCaaaa                                                                                                                                                 >  2:256566/1‑151 (MQ=255)
    aaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCaaaa                                                                                                                                                 >  2:527955/1‑151 (MQ=255)
    aaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCaaaa                                                                                                                                                 >  1:517324/1‑151 (MQ=255)
      aTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAAc                                                                                                                                               <  1:1318054/151‑1 (MQ=255)
         aTCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTCCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCg                                                                                                                                             >  2:1424058/1‑150 (MQ=255)
             cATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCAtt                                                                                                                                        >  2:909950/1‑151 (MQ=255)
               tGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAg                                                                                                                                      >  2:769118/1‑151 (MQ=255)
                 aaGATGTTTTGTAATTGTGTTGGTTGA‑CATTCATATAAAAAAAACCAAATTCCCATCATGTTTGTGTGGGTTTTTTTCTGTATCTTACGCCCCGCACCCAACCCTAACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCt                                                                                                                                    <  1:1044350/150‑1 (MQ=255)
                         ttGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGa                                                                                                                            >  1:677147/1‑151 (MQ=255)
                         ttGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGa                                                                                                                            >  2:899281/1‑151 (MQ=255)
                          tgtgAATTTTTTGGTGGA‑CTTTCATATAAAAAAAATCAAAATCCCATCATTTTTGTGTGGGTTTTTTTCTGTATCTTACCCATCGCACTCAACCCTGACAAAAAATATGT‑TGTAAGGC‑TAATATAAAAAACCGCCATTAGCTCATCAGGaa                                                                                                                           <  2:899501/151‑1 (MQ=255)
                           gtgAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAag                                                                                                                          >  1:307918/1‑151 (MQ=255)
                            tgAGTGTGTTGTTTGA‑CATTAATATAAAAAAAATCATAATCCCATCATGTTTGTGGGGGGTTTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAAAAAATAGGC‑TGTAAGGC‑TCATAACAAAACCCGCCATTAGCTCATCAGGAag                                                                                                                          <  1:1155411/150‑1 (MQ=255)
                            tgAATGTTTTGGTTGC‑TTTTCATAGAAAAAAAATCAAAAACCCATAATGTTTGTGGGGGTTCTTTTCTTTATCTTCCGCACCGCACACAAGCCTGAAAAAAAATATCC‑TTGAAGGC‑TAATATAAAAACCCGCCATTAGCTCATCAGGAag                                                                                                                          <  2:513066/150‑1 (MQ=255)
                            taaaTGTGTTGGTTGA‑CATTAATAGAAAAAAAATCATAATCCCATCATGTTGGGGGGGGGTTTTTTCTTTATCTTACGCACCCCACTCAAGCCTGAAAAAAAATATGT‑TGAAGGGC‑TCAAATAAAAAACCGCCATTAGCTCATCAGGAag                                                                                                                          <  1:166918/148‑1 (MQ=255)
                            taaaTGTGTTGGTTCA‑CATTCATAAAAAAAAAATAATAATCCCATCGGGTTTGGGGGGGTTCTTTTTTGGATCTTCCGCCTCCCCCAAAAGCCGGGAAAAAAAT‑TGCTTGAAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAag                                                                                                                          <  2:512142/148‑1 (MQ=255)
                                 gtgtTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAga                                                                                                                    >  2:895221/1‑151 (MQ=255)
                                 gtgtTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAga                                                                                                                    >  2:588891/1‑151 (MQ=255)
                                  ttttggtttACCATTAATATAAAAAAAATCATAATCCCATCATGTTGGTGGGGGTTCTTTTTTGTATTTTCCGCACCGCACCCAAGCCTAAAAAAAAAAATGC‑TGAAAGGC‑TAAAAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAg                                                                                                                     <  1:336568/148‑1 (MQ=255)
                                   tttggttgA‑CATTAATATAAAAAAAATAATAATTCCATCATGTTTGTGTGGGTTTTTTTCTGTTTCTTACGCACCCCCCTCAAGCCTGACAAAAAATTTGC‑TGAAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacg                                                                                                                  <  2:419484/150‑1 (MQ=255)
                                   tttggttgA‑ATTTCATATAAAAAAAATCAAAATCCCATCATGTTTGTGTGGGTTTTTTTCTGTATCTTCCACACCCCCCCCAACCCAGAAAAAAAATTTGT‑GGAAAGGC‑TCAAAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacg                                                                                                                  <  1:45455/150‑1 (MQ=255)
                                   gttggttgA‑CATTCATATGAAAAAAATCATAATTCAATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacg                                                                                                                  >  2:1252837/1‑151 (MQ=255)
                                    ttggtggA‑CATTCATATAAAAAAAACCATAATCCAATAATGTTGGTGGGGGTTTTTTTCTGTACCTCCCCCACCCCCCCCAACCCTAAAAAAAAAAATGT‑GGAAAGCT‑TAATACAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacg                                                                                                                  <  1:394458/150‑1 (MQ=255)
                                      ggttgA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACa                                                                                                               >  1:377950/1‑151 (MQ=255)
                                         ttaatttttaattaaaaaaaaTCAAATTCCCATCATTTTTGTGTGGGCTCTTTTTTATTTTTCACGCTCCGCCCAAAAGCCTAAAAAAAAAAATGC‑AGTAAGGC‑TCAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAc                                                                                                             <  2:200736/137‑1 (MQ=255)
                                          aa‑CATTCATATAAAAAAAATCAAAATCCCACCATTTTTGGGGGGGTTTTTTTCTGTTTCTTACGCATCGCCCCAAAGCCTAAAAAAAAATATGC‑TTTAAGGC‑TAATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCa                                                                                                           <  2:1193822/150‑1 (MQ=255)
                                              attaatagaaaaaaaaTAAAAATTCCATCATTTTTGTGGGGGTTTTTTTCGGTATCTTACGCATCCCCCTCAAGCCTAAAAAAAAATATGC‑TGTAAGGC‑TAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGa                                                                                                        <  2:1470685/142‑1 (MQ=255)
                                               ttaaaataaaaaaaaCAATAATTCCATCATGTTTGGGTGGGTTTTTTTCGTAATCTAACCCATCCCACACAAGCCTAAAAAAAAATATGC‑TGAAAGCC‑TAAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt                                                                                                       <  1:1446054/143‑1 (MQ=255)
                                               ttCATATAAAAAAAATCAAATTCCCATATGGTTGGTGGGGGTTTTTTTTTTTATCTGACGCCTCACACACACCCCTGACAAAAAATATTC‑TGAAAGGC‑TCAAAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt                                                                                                       <  1:1186710/151‑1 (MQ=255)
                                                taatatGAAAAAAATCATAATCCCATCATGTTTGTGTGGGGTTTTTTCTTTATCTTACGCATCCCACCCAAGCCGAAAAAAAAATAGGC‑GGAAGGCC‑TAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt                                                                                                      <  1:246702/149‑1 (MQ=255)
                                                    ataaaaaaaaTCATAATCCCATCATTTTTGTGTGGGGTTTTTTCTTTATCTTACGCACCGCCCCAAAGCCTGAAAAAAAATATGC‑TGTAAGGC‑TCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgttg                                                                                                  <  2:1089000/148‑1 (MQ=255)
                                                    ataaaaaaaaTAATAATCCCATCATGTTTGTGGGGGTTCTTTTCTGTATCTTACGCACCGCACTCAAGCCTGACAAAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgttg                                                                                                  <  2:1484648/148‑1 (MQ=255)
                                                    ataaaaaaaaCCATAATCCAATCATTTTTGTGTGGGTTCTTTTCTGTATCTTACCCATCGCACTCAAGCCTGAAAAAAAATATGC‑TGTAAGCC‑TCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgttg                                                                                                  <  1:559461/148‑1 (MQ=255)
                                                       aaaaaaaTCATAATTCCAGCATGTTTGGGGGGGTTTTTTTCTGTAGTTTACCCACCGCCCACAAGCCTGACAAAAAATATGT‑TGAAAGCC‑ACATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTAt                                                                                               <  1:380725/151‑1 (MQ=255)
                                                         aaaatacATATTCCCATCATGTTTGTGGGGGTTTTTTTCTGTATCTAACGCATCGCACTCAACCTTGAAAAAAAATAGGT‑TGTAAGGC‑TAATATCAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                             <  1:1180549/145‑1 (MQ=255)
                                                         aaaatTCATATTCCCATCTTGTTTGTGTGGGTTTTTTTTTGTATCTTACTCACCGCCCTCAACCTTGACAAAAAATATGT‑TG‑AAGGCTTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                             <  1:159803/151‑1 (MQ=255)
                                                         aaaacTAATAATTCTATAATGTTTGGGGGGGTTTTTTTCGTTTTCTTGCGATGCACCCGAAAGCCTAAAAAAAAATGTTG‑TTTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                             <  2:1132726/151‑1 (MQ=255)
                                                         aaaaaTCATAATCCCATCATGTTTGTGGGGGGTCTTTTCTTTTTCTTACCCATCGCACTAAAGCCTAAAAAAAAATTTGC‑GGTAAGCC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                             <  2:867629/151‑1 (MQ=255)
                                                         aaaaaTCAAATTTCCATATGGTTTGTGTGGGGTCTTTTTTGTATTTACCGCATCCCACCAAAGCTGGACAGAAAAAATGG‑TGGAAGGC‑GAATATAAAAATCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                             <  1:1056476/151‑1 (MQ=255)
                                                         aaaaaTCAAAATCCCATTATTTTTGGTTGGGTTTTTTTCTTTTTCTTCCCCACCCCCCTAAAGCCTGAAAAAAAATATGT‑TGAAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                             <  2:499030/151‑1 (MQ=255)
                                                         aaaaaTAATATTCCCATATTGTTTGGTGGGGGTTTTTTTTGAATCTTACGCGCCCCACTAAACCCGAAAAAAAAATGTGT‑TGTAGGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                             <  2:185117/151‑1 (MQ=255)
                                                          aaaaTCATAATCCCATCGTTTTTGTGTGGGTTTTTTTCTGTATCTAACGCACCCCACAAAGGCCTAAAAAAAAATATGG‑TGAAGGCC‑TAAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGt                                                                                            <  1:830944/151‑1 (MQ=255)
                                                            aacaaaatttCCACCATTTTTGTGGGGGGTTTTTTCTTTATCTTCCGCACCGCACCAAAGCCTGACAAAAAATATGC‑GGAAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTAc                                                                                          <  2:12012/143‑1 (MQ=255)
                                                                atactccaatcatGTTTGTGTGGGGTCTTTTCTGTATCTTACACACCGCACTCAAGCCTAACAAAAAATATGC‑TGTAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACgggg                                                                                      <  1:17902/143‑1 (MQ=255)
                                                                 tAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGt                                                                                     >  1:506380/1‑151 (MQ=255)
                                                                 tAATCCCATCATGTTTGTGTGGGTTTTTTTCTGTATTTTCCGCACCGCACTCAAGCCTGAAAGAAAATATGC‑TGTAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGt                                                                                     <  2:201095/151‑1 (MQ=255)
                                                                  aaTCCCATCATGTTTGTGGGGGGTTTTTTCTGTATCTTACGCACCCCACTCAACCCTGACAAAAAATATGC‑TGAAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGtt                                                                                    <  2:86417/151‑1 (MQ=255)
                                                                      ccATCATGTTTGGGTGGGGTTTTTTCTGTATTTTACGCATCGCACAAAAGCCGCAAAAAAAATATGC‑TGAAAGGC‑TCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAg                                                                                <  2:147450/151‑1 (MQ=255)
                                                                       catcatTTTTGTGGGGGTTTTTTTTTTTTTTTTCCGCACCGCACCAAAGCCTGACAAAAAATATGT‑TGAAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACATCCAGGATTGTTGTATGGTACGGGGTTCGAgg                                                                               <  2:774658/151‑1 (MQ=255)
                                                                           atGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                            >  2:738439/1‑150 (MQ=255)
                                                                           atGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                            >  1:1490385/1‑150 (MQ=255)
                                                                           atGTTTGCGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                            >  1:1123215/1‑150 (MQ=255)
                                                                           atGGTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACTAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                            >  1:1049820/1‑150 (MQ=255)
                                                                              tttGTGGGGGTTTTTTTCTTTATCTACCGACCCCCACCAAAGCCTAAAAAAAAATTTGT‑TGAAAGGC‑TAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGAt                                                                        <  2:314324/151‑1 (MQ=255)
                                                                               ttgggggggtttttttttttATTTTCCGCATCGCACTAAAGCTTGCCAAAAAATATGC‑TGAAAGGC‑TAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATg                                                                       <  2:1372568/139‑1 (MQ=255)
                                                                                tgtgggGGGTTTTTTCTTTATCTTCCGCACCCCCCTCAACCCTGACAAAAAATATGT‑TGTAAGGC‑TAATAACAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATgg                                                                      <  2:97139/151‑1 (MQ=255)
                                                                                     ggggTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGt                                                                  >  2:122020/1‑150 (MQ=255)
                                                                                        gTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCaa                                                              >  2:820752/1‑151 (MQ=255)
                                                                                            tttCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACttt                                                          >  2:524320/1‑151 (MQ=255)
                                                                                                 gTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaa                                                     >  2:72182/1‑151 (MQ=255)
                                                                                                   ttCTTCCGCACCGCACTAAACCCTAAAAAAAAATATGT‑TGTAAGGC‑TAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAAc                                                   <  2:1361674/150‑1 (MQ=255)
                                                                                                   ttCTTACCCACCCCCCCCAACCCTGAAAAAAAATTTGC‑TGAAAGCC‑TAATAAAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAAc                                                   <  2:1247233/150‑1 (MQ=255)
                                                                                                   aTCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa                                                    >  2:808441/1‑150 (MQ=255)
                                                                                                   aTCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa                                                    >  2:438928/1‑150 (MQ=255)
                                                                                                   aTCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa                                                    >  2:130689/1‑150 (MQ=255)
                                                                                                   aTCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa                                                    >  1:358274/1‑150 (MQ=255)
                                                                                                   aTCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa                                                    >  1:49978/1‑150 (MQ=255)
                                                                                                   aTCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa                                                    >  1:69924/1‑150 (MQ=255)
                                                                                                     cTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAAct                                                  >  2:1264641/1‑150 (MQ=255)
                                                                                                              cGCACCCAACCTTAACAGAAAATAGGA‑TGAAGGGC‑GAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                        <  1:420201/151‑1 (MQ=255)
                                                                                                                cACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAg                                      >  2:873906/1‑151 (MQ=255)
                                                                                                                   caacgcttGACAAAAAATTGGC‑TGAAAGCC‑TAATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATc                                   <  1:1396864/144‑1 (MQ=255)
                                                                                                                     aaccctaaaaaaaaaTATGC‑TGAAAGCC‑TAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATccc                                 <  2:1012808/140‑1 (MQ=255)
                                                                                                                           gACAGCAACTATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAACAGGAATGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttatt                           >  1:1277447/1‑151 (MQ=255)
                                                                                                                            aCAAAAAATATGC‑TGAAAGGC‑TCTTACAAAACGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttatta                          <  1:212720/151‑1 (MQ=255)
                                                                                                                            aCAAAAAAGATGC‑TGAAGGCC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttatta                          <  1:135216/151‑1 (MQ=255)
                                                                                                                             aaaaaaaTTTGC‑TGAAAGGC‑TAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTtattat                         <  2:1270571/148‑1 (MQ=255)
                                                                                                                             aaaaaaaTATGC‑TGTAAGGC‑TAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTtattat                         <  1:1506537/148‑1 (MQ=255)
                                                                                                                             aaaaaaaTATGC‑AGTAAGGC‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTtattat                         <  1:1239014/148‑1 (MQ=255)
                                                                                                                              agaaaagaaggtagaaaGGT‑TCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttatta                          <  1:433608/135‑1 (MQ=255)
                                                                                                                              aaaaaaaaTGC‑TGAAAGGT‑TAATACAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatc                        <  2:955317/149‑1 (MQ=255)
                                                                                                                               gAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttatta                          >  2:433758/1‑148 (MQ=255)
                                                                                                                               gAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatca                       >  2:1289709/1‑151 (MQ=255)
                                                                                                                               gAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatca                       >  1:1312365/1‑151 (MQ=255)
                                                                                                                               gAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatca                       >  1:949004/1‑151 (MQ=255)
                                                                                                                                 aaaTATGC‑TGAAGGGC‑TAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcatc                     <  2:1298624/151‑1 (MQ=255)
                                                                                                                                 aaaTATGA‑TGTAGGGG‑TCAGATAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcatc                     <  1:1269606/151‑1 (MQ=255)
                                                                                                                                 aaaTAGGC‑TGTAAGGC‑TCATATAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcatc                     <  1:362774/151‑1 (MQ=255)
                                                                                                                                  aaTATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGCATGGTACGGGGt                                                                                     >  1:1009040/1‑86 (MQ=255)
                                                                                                                                       gC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa               >  2:496122/1‑151 (MQ=255)
                                                                                                                                       gC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa               >  1:192606/1‑151 (MQ=255)
                                                                                                                                       gC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa               >  1:1376290/1‑151 (MQ=255)
                                                                                                                                        c‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaa              >  2:1153158/1‑151 (MQ=255)
                                                                                                                                           gTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAg            >  1:1446004/1‑151 (MQ=255)
                                                                                                                                            aaaGGC‑TAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGt           <  2:378071/150‑1 (MQ=255)
                                                                                                                                             aagggtaaatatCAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGt           <  1:1152614/143‑1 (MQ=255)
                                                                                                                                               ggC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGa        >  2:1118166/1‑151 (MQ=255)
                                                                                                                                               ggC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGa        >  1:295890/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                   
TAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGA‑CATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGC‑TGTAAGGC‑TCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTG  >  NC_000913/345178‑345475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑