Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,337,7230AC50.0% 103.6 / 30.2 82G566G (GGT→GGGgyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base A (38/3);  new base C (0/41);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 6.24e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTAC  >  NC_000913/2337574‑2337868
                                                                                                                                                     |                                                                                                                                                 
ccGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTa                                                                                                                                                   >  2:1151265/1‑150 (MQ=255)
 cgcgcgcgCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTAcc                                                                                                                                                 <  1:1099975/151‑1 (MQ=255)
     cgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTc                                                                                                                                             <  2:1056180/151‑1 (MQ=255)
       cgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTcc                                                                                                                                            >  1:1457710/1‑150 (MQ=255)
           aCGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCa                                                                                                                                       >  2:1280195/1‑151 (MQ=255)
                      cggccAATTGAAAAACTTTCACAGAATGGAGGCCCCCAGGGCGGGAGAAGCACAAAATAGGGTGGGGAGTGTCCGCCACCAGCAGTCGGTCAAAAAAGTCTTCTTCTTTAAGGCGTGCGGCAGTTTTCCCTTTCCCGCCACGACGCTgcgc                                                                                                                            <  1:1406200/147‑1 (MQ=255)
                         gCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCtt                                                                                                                          >  2:1427179/1‑150 (MQ=255)
                           aattaaaaaaCTTTCATCGAAAAGACGCGCCCCCGGCTGGAAAAGCAAAAAATATGGTCGGGAGTTTTCCCCCCCAGCAGTCGGTCGAAAAAGTCTTCTTTTTTAATACGTGCGGCAGATTTCCTTTTCCCGCCACGACGCTGCGCTTCGt                                                                                                                       <  2:1247721/144‑1 (MQ=255)
                            aCTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTa                                                                                                                      >  1:203955/1‑151 (MQ=255)
                             cTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTAt                                                                                                                     >  2:1336993/1‑151 (MQ=255)
                             cTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTAt                                                                                                                     >  2:1307359/1‑151 (MQ=255)
                             cTGAAAAACTTTCATCGAATAGACGCGCCCACGGCGGGAAAAGCACAGAATATGGTGGTGAGTTTTCGCCACCAGAAGTGGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTAt                                                                                                                     <  2:1109847/151‑1 (MQ=255)
                               gATAACCTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGACTTACCTTTCCCGCCACGACGCTGCGCTTCGTAtt                                                                                                                    >  1:1221907/1‑150 (MQ=255)
                               aaTAACCTTCCACCAAATGGACGCCCCCACGGCTGGAGAAGCAAAGAATATGGTCGTGGGTTTCCGCCACCAGCAGTCGGTCAATAAGGTCTTCTTCTTTAATACGGGCGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTc                                                                                                                   <  2:1087970/150‑1 (MQ=255)
                                  aaccTTTCATGGAATAGCCGGACCCCCGGCTGGGGANNNNANNAATGTGGGCGTGAGTGTCCCCCCCCACCAGTCGTTCGATAAATTCTTTTTCTTTATTACGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGa                                                                                                                <  1:1457116/148‑1 (MQ=255)
                                             gAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGAt                                                                                                     >  2:535551/1‑151 (MQ=255)
                                             gAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGAt                                                                                                     >  1:1510379/1‑151 (MQ=255)
                                               aTAGACGCCACCGCGGGGGGAAAAGAACAGAATATGGTGGGGAGTGTCCGCCACCAGAGGTCGGTCAAAAAATTCTTCTTTTTAAATCCGGGCGGAAAATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATAc                                                                                                   <  1:229649/151‑1 (MQ=255)
                                                  gACGCGACCACGGCTGGCGAAGCACAGAATATGGTCGTGAGTGTTCCCCACCAGCAGTCGGTCGCTAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTa                                                                                                >  1:863195/1‑151 (MQ=255)
                                                  gACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTa                                                                                                >  2:485114/1‑151 (MQ=255)
                                                  gACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGGCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTa                                                                                                >  1:1395449/1‑151 (MQ=255)
                                                    cgcgCCCACGGCTGGAGAAGAAAAAAATATGGTCGTGAGTGTTCGCCCCCAGCAGTCGGTCGAAAAAGTCTTCTTTTTAAAGACGTGCGGAAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAAc                                                                                              <  1:88191/151‑1 (MQ=255)
                                                      cgcccccggcggGAGAACCACAAAATAGGGTCGGGGGGGTTCGCCCCCAGCAGCCGGTCGAAAAAGTTTTTTTTTTTAATACGGGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGt                                                                                            <  2:611541/145‑1 (MQ=255)
                                                      cgcccACGGCGGGAGAAACACAGAATTTGGTCGTGAGTTTTCGCCCCCAGCAGTCGGTCGATAAAGTTTTTTTTTTAAATCCGGGGGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGt                                                                                            <  2:369616/148‑1 (MQ=255)
                                                      cgACCCCGGCGGGAAAAGCACAGAATATGGTGGGGAGTGTTCCCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGGGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGt                                                                                            <  2:1025135/151‑1 (MQ=255)
                                                      cacccAGGGGTGGAGAAGAACAAATTATGTTCGTGAGTGTGCGCCACCAGCAGTCGGTAGATAAGGTCTTCTTTTTTAATACGGGCGGCAGTTTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGt                                                                                            <  1:486950/148‑1 (MQ=255)
                                                             ggCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGcc                                                                                        >  1:1031962/1‑148 (MQ=255)
                                                                 ggAGAAGCAGAGAATTTGTGGGGGGGGGTCCCCCCCCAGCTGTCGGTCAATAAGGTTTTTTTTTTTAAGCCGGGGGGAGTTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTg                                                                                  <  1:186676/150‑1 (MQ=255)
                                                                 ggAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTga                                                                                 >  1:160867/1‑151 (MQ=255)
                                                                 ggAGAAGCAAAAAATTTGGTCGTGAGTGTTCCCCCCCAGCAGTCGGTCGATAAAGTTTTCTTCTTTAATGCGGGCGGCAAATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTg                                                                                  <  2:344874/150‑1 (MQ=255)
                                                                 ggAAAAGCAAAAAATAGGGTGGGGAGTTTTCCCCCCCAGCAGTCGGTCAATAAAGTCTTTTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTg                                                                                  <  2:989168/150‑1 (MQ=255)
                                                                   agaAGCAAAGAATAGGGTGGTGATTGTCCCCCCCCAGCGTTCGGTCGATAAAGTTTTCTTTTTTAATACGGGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag                                                                                <  2:1386982/150‑1 (MQ=255)
                                                                   aaaaGCACAAAATAGGGCCGTGAGTGTCCCCCCCCAGCAGTCGTTCGAAAAAGTTTTTTTTTTTAATCCGGGGGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag                                                                                <  2:926012/148‑1 (MQ=255)
                                                                    aaaGCACAGAATATGGTCGGGAGTGTTCCCCCCCAGCAGTCGGTCGAAAAAGTCTTCTTTTTTAATACGTGCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagag                                                                              <  2:452097/150‑1 (MQ=255)
                                                                     aaGCGCAGAATATGGTCGGGTGTGTTCGCCCCAAGCGTCCGGTTATAAAAGTCTTTTTTTTTATTTCGGGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagag                                                                              <  1:391051/150‑1 (MQ=255)
                                                                       gCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGagagag                                                                            >  1:917011/1‑150 (MQ=255)
                                                                       gCACAGAATATGGTCGGGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGagagag                                                                            >  2:610727/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAGGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                                       >  2:365764/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                                       >  2:1342371/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                                       >  1:492082/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                                       >  2:1025958/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                                       >  2:277689/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                                       >  1:41110/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                                       >  1:1423689/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa                                                                       >  1:360695/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGGGAGAGAGCGTCa                                                                       >  1:1083186/1‑150 (MQ=255)
                                                                            gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGCGAGAGAGCGTCa                                                                       >  1:294365/1‑150 (MQ=255)
                                                                              atatGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGa                                                                    >  2:530743/1‑151 (MQ=255)
                                                                              atatGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGa                                                                    >  1:1053213/1‑151 (MQ=255)
                                                                              atatGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGa                                                                    >  1:866837/1‑151 (MQ=255)
                                                                                  ggTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACca                                                                 >  1:1450692/1‑150 (MQ=255)
                                                                                   ttcgggggttttCCCCCCCGGCAGTCGGTCAAAAAAGTCTTTTTTTTAAATCCGGGCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACcac                                                                <  2:1400756/140‑1 (MQ=255)
                                                                                    tCGTGAGTTTCCCCCCCCAGCAGTCGGTCGATAAAGTCTTCTTTTTTAATACGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACAt                                                              <  2:41136/151‑1 (MQ=255)
                                                                                      gTGAGTGTCCCCCCCCAGCAGTGGGTCAAAAAAGTCTTTTTCTTTAATACGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATc                                                             <  2:371255/150‑1 (MQ=255)
                                                                                         agtttccccccccaGCGGTCGGTCGATAAATTCTTTTTTTTTAATCCGTGGGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTc                                                          <  2:142096/139‑1 (MQ=255)
                                                                                          gtgtTCCCCCCCAGCAGTGGGTGAAAAAAGTCTTCTTCTTAAATACGTGCGGCAGTTTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTcc                                                         <  2:1386408/150‑1 (MQ=255)
                                                                                           tgtTCGCCCCCAGCAGTCGGTCAAAAAAGTCTTCTTTTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt                                                        <  2:360802/150‑1 (MQ=255)
                                                                                             ttCGCCACCCGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCATggg                                                     >  1:275273/1‑151 (MQ=255)
                                                                                             tcccccccgagcagTCGGTCGAAAAATCTTTCTTCTTAAATGCGTGCGAAATTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTggg                                                     <  1:71240/142‑1 (MQ=255)
                                                                                             tccccccccaGCGGTGGGTCAATAAAGTCTTTTTCTTTAATCCGGGCGGCAATTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTggg                                                     <  2:1032470/144‑1 (MQ=255)
                                                                                             tccGCCCCCGGCAGCCGGTCAAAAAAGTTTTCTTCTTAAATACGGGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTggg                                                     <  1:1279583/149‑1 (MQ=255)
                                                                                             tccGCCACCAGCGGTGGGTCGAAAAATTTTTTTTCTTTAAGACGTGCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTggg                                                     <  1:1414364/149‑1 (MQ=255)
                                                                                                  ccccggcagTCGTTGAATAAAGTCTTCTTTTTAAATCCGGGGGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATc                                                <  1:594745/146‑1 (MQ=255)
                                                                                                   cccaGCTGTCGGTCGATAAATTCTTTTTCTTTAATCCGTGCGGCAATTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCa                                               <  1:679251/150‑1 (MQ=255)
                                                                                                        cggtcggtcaATAAATTTTTCTTTTTTAATACGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATc                                           <  2:934995/148‑1 (MQ=255)
                                                                                                           tcgttcgagaagctattctttttTATTGCGTCCGGCATATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTc                                        <  1:724063/135‑1 (MQ=255)
                                                                                                           tcggtcgAAAAAGTTTTTTTTTTTAATACGGGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc                                       <  2:450099/151‑1 (MQ=255)
                                                                                                           tcggtcgAAAAAGTCTTCTTCTTTAATGCGTGGGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc                                       <  2:1086676/151‑1 (MQ=255)
                                                                                                            cggtcgATAAAGTCTTTTTTTTTAATCCGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCa                                      <  2:1262746/151‑1 (MQ=255)
                                                                                                               tcgATAAAGTCTTTTTTTTTAATACGTGCGGAAGTTTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAgg                                    <  1:1336335/150‑1 (MQ=255)
                                                                                                               tcaaTAAAGTCTTTTTTTTTAATACGGGCGGAAAATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGt                                   <  2:239398/148‑1 (MQ=255)
                                                                                                                    aaaGTCTTTTTTTTTAATCCGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATg                              <  2:720780/151‑1 (MQ=255)
                                                                                                                       gTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGCCGCTGCGCTTCGTATTCAGAAAGCGGCTGCTCCCTACCGTAGCCCTGGTGCGAGAGCGTCACGACCACATCTTCCTGGCCGATCAGATCTTCCAGGTTGATGt                             >  1:301468/1‑149 (MQ=255)
                                                                                                                             ttttttAATACGTGCGGCATTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTg                     <  2:268766/148‑1 (MQ=255)
                                                                                                                             ttttttAATACGTGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTg                     <  2:340605/148‑1 (MQ=255)
                                                                                                                              tcttTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgcg                        >  2:1212002/1‑147 (MQ=255)
                                                                                                                              tcttTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgcg                        >  1:529973/1‑147 (MQ=255)
                                                                                                                               ctctAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGtt                   >  2:1200035/4‑151 (MQ=255)
                                                                                                                                                taTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTac  <  1:1313994/150‑1 (MQ=255)
                                                                                                                                                gaattACCTTTCCCGCGATGACGAAGCGCTTCGTATTGAGAAAGCGGCTGATACTTAACGTAGCACTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGCGAGTTCAGTa   >  1:460341/4‑150 (MQ=255)
                                                                                                                                                gATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTa   >  2:822785/1‑150 (MQ=255)
                                                                                                                                                gATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTa   >  1:167669/1‑150 (MQ=255)
                                                                                                                                                aaTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTac  <  2:1083710/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTAC  >  NC_000913/2337574‑2337868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: