Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC48.0% 101.4 / 29.7 76V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (34/5);  new base C (0/36);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 3.40e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443131‑3443427
                                                                                                                                                      |                                                                                                                                                       
aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccc                                                                                                                                                     <  1:256164/150‑1 (MQ=255)
       gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTTCCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                             >  2:504382/1‑151 (MQ=255)
       gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                             >  2:721452/1‑151 (MQ=255)
       gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                             >  1:1430745/1‑151 (MQ=255)
       gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                             >  2:1172225/1‑151 (MQ=255)
       gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGACTCCGGGAACAGAATAATACTGGAACCGAAGATTTCCGGGATTACCCCCGCCata                                                                                                                                             >  2:573785/1‑151 (MQ=255)
         gTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATAtt                                                                                                                                           >  2:763456/1‑151 (MQ=255)
          aaaCACAAAAGGGGTTTCCCCGGGCTAAAAAAAAAGCAAATTTGTTGTCCCCCCTTCCCACCAAGCCCCGCCCCCGACCCAGGCCGCGGTGGTCCCGGGAAACAAAATAATCCTGAAAGGAAAGATGGCGGGGATTACCCCCGCCATATTc                                                                                                                                          <  1:406360/150‑1 (MQ=255)
            acacATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCa                                                                                                                                         >  2:1227327/1‑150 (MQ=255)
             cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCa                                                                                                                                         >  1:287118/1‑149 (MQ=255)
             cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCa                                                                                                                                         >  1:1417091/1‑149 (MQ=255)
             cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGATCCAACCAGTACCGCCCCCGAACCAAGACGCGATGGTCGCCGGGCACAGAATAATATTGGAAGCGAAGATTGACGGGATTACACCCGCCATATTCa                                                                                                                                         >  1:1441601/1‑149 (MQ=255)
                           gCCCAGGCTGCAAATACAGCGAAATTTTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         >  2:557794/1‑151 (MQ=255)
                           gCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         >  2:1199566/1‑151 (MQ=255)
                           gCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         >  2:947685/1‑151 (MQ=255)
                           gCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         >  1:887580/1‑151 (MQ=255)
                           ccccgggcgaaaaaaACAGCAAATTTGTTGCCACCCTGTCCCACCCATTACCCCCCCCGACCAAGAAGGCGAGGCACCCCGGAAAAAAAAAAATTTTGGAAGGAAAGGTTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         <  1:1487281/140‑1 (MQ=255)
                                ggcaaaaaaTACAGCAAATTTGTTGTCAGCCGTTCCCACCAGGCCCCCCCCCCAAACCAGGACGGGTTGGCCCCGGGGAAAAAAAAAAACCGGGAAGGGAAGTTGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                       <  2:1382960/142‑1 (MQ=255)
                                 cctacAAACACAGAAAATTTGTTGCCCCCCATTCCAACCCGGCCCCCCCCCCAACCCAGGCCGCGGGGGTCGCGGGAAAAAAAAAAATCTGGGAAGAAAAGTTGGCGGGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                    <  1:454196/146‑1 (MQ=255)
                                  cTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                   >  2:266639/1‑150 (MQ=255)
                                    gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                   >  2:507939/1‑148 (MQ=255)
                                    gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGCAAATGTGTGct                                                                                                                   >  1:28985/1‑148 (MQ=255)
                                          acggaaatgttgttgTCACCCGTTCACACCCACTCCCCCCCCAAACCCATGGGGCGAGTGGCGCCGGAAAAAAAAAAACCCGGAAAGCAAAGTTGGCCGGGTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                          <  1:1231600/142‑1 (MQ=255)
                                             gcaataTTGTTGTCAGCCATTCCCAACAAGCCCCCCCCCATAACCGGGACGGGCTGGCCCCGGGAAACAGAATAATACGGAAGCCGAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        <  1:889149/145‑1 (MQ=255)
                                              caaatttgttgCCCGCCAGTCCCAACCAGCCCCCCCCCCAAACAATGGGGCGTGGGCCGCGGGGAACAAAATAATACGGGAAGCGAAGATGGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        <  2:838369/144‑1 (MQ=255)
                                              cGAAATTGTTGCCAGCAGGTCCCAACCACTCCCCCCCCCAAACCATGACGCGTTGGTCCCCGGGAAAAAAATAATCCTGGAACGAAAGTTTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        <  1:1377566/149‑1 (MQ=255)
                                              agAATTTGTTGTCGGCCGTTTCCACCCAGTCCCCCCCCCGACCGAGGGCGGGGGGGTCGCGGGGAAAAAAAAAATACGGGAAGGGAAGATGGGGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        <  1:1278794/148‑1 (MQ=255)
                                                aactttttgcCAGCCAGTTCCAACAATCCCCCCCCCCAACCCATGACGGGATGGTCGCCGGAAAAAAAATAATCCGGGAGGCAAAGATTGCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                    <  1:1254032/145‑1 (MQ=255)
                                                 atttgttgcaggCCAGTCCCAACCGGCCCCCCCCCCGAACCAGGCGGGGAGGGTCGCGGGAAAAAAAAAAATACTGGAAGGGAAAATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                   <  2:345332/140‑1 (MQ=255)
                                                 atttgttgTCAGCCAGTCCCAACCAGTCCCCCCCCCAAACCGGGACGGGAGGGTCGCCGGAAACAAAATAATATTGGAGGGAAAGTTGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                   <  1:81898/149‑1 (MQ=255)
                                                 aaTTTTTGGCCGCCAGTCCAAACCAGACCCGCCCCCGAACCATGACGCGAGGGTCGCCGGAAACAAAATAATCTGGGAGGCGAAGTTGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                   <  1:620204/151‑1 (MQ=255)
                                                  tttgttgCCACCCAGTCCCAACAAGCCCCCCCCCCGACCCATGACGGGAGGGTCCCCGAGAAAAAAATAATACGGGAAGAGAAGTTGGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                  <  1:1306302/150‑1 (MQ=255)
                                                  aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGATCTGTGCAGCATAGaca                                                                                                   >  1:114958/1‑150 (MQ=255)
                                                   ttgtggCCAGCCAGTCCCACCCATACCCGCCCCCTAACCAGGGGGCATTGGGCGCCGGGAAAATAATATTCTTGAAAGCGAGTATTGCCGGAATACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                   <  1:19089/149‑1 (MQ=255)
                                                     gttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                                >  2:83024/1‑150 (MQ=255)
                                                           aGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                         >  1:352510/1‑151 (MQ=255)
                                                           aGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                         >  2:896102/1‑151 (MQ=255)
                                                                gTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGa                                                                                    >  2:1363265/1‑151 (MQ=255)
                                                                gTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGa                                                                                    >  2:1265242/1‑151 (MQ=255)
                                                                            gTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                          >  2:951641/1‑151 (MQ=255)
                                                                                  cccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTa                                                                    >  2:1247388/1‑151 (MQ=255)
                                                                                      cGACCCAGGCCGGGAGGGCCGCGGGGAAAAAAATAATACTGAAAGCAAAGTTGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                <  2:1219992/151‑1 (MQ=255)
                                                                                      cGAACCATGCCGCGATGGTCGCCGGAAAAAAAATAATACGGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                <  2:1417814/151‑1 (MQ=255)
                                                                                      cGAACCAGGACGCGGTGGTCGCGGGGAAAAAAAAAATACGGGAAGCAAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                <  2:147597/151‑1 (MQ=255)
                                                                                       cgacccaggcggggttGGTCGCGGGAAAAAAAATAATGCTGGAGGCAAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                <  1:1428646/136‑1 (MQ=255)
                                                                                       cgaccaaagccggggtGGCCGCGGGAAACAAAATAATACAGAAAGAAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                <  1:1198994/136‑1 (MQ=255)
                                                                                       cgaacaaggcggggATGGCCGCCGGGAACAAAATAATACTGGAAGGGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                <  1:601236/138‑1 (MQ=255)
                                                                                       caacccATGACGGGATGGCCGCGGGAAAAAAAATAATAGTGGAAGGAAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                <  2:312853/147‑1 (MQ=255)
                                                                                       caaaccagggggggATGGCCGCGGGAAAAAAAAAAATACGGGAAGCGAAGTTGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                <  2:1110417/138‑1 (MQ=255)
                                                                                        aaCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACaa                                                              >  2:227584/1‑151 (MQ=255)
                                                                                         aCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                             >  2:1502039/1‑151 (MQ=255)
                                                                                                            cggggAACAAAATAATACGGAAGGCAAAGTTTGCCGGGATCCCCACCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                            <  1:1178002/148‑1 (MQ=255)
                                                                                                                gAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                       >  1:673477/1‑151 (MQ=255)
                                                                                                                aaaaaaaaaaatccgggAAGAGAAGTTGGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                       <  2:680413/136‑1 (MQ=255)
                                                                                                                     gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   >  2:245631/1‑150 (MQ=255)
                                                                                                                     gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   >  1:603203/1‑150 (MQ=255)
                                                                                                                     gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   >  2:1408909/1‑150 (MQ=255)
                                                                                                                     gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   >  2:847705/1‑150 (MQ=255)
                                                                                                                     gAATAATACTGGAAGCGAAGATTGCCGGGATTAACCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   >  1:1214990/1‑150 (MQ=255)
                                                                                                                     aaatactACGGGAGACGAAAGTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  <  1:424379/150‑1 (MQ=255)
                                                                                                                     aaataatATTGAAGGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  <  2:1261568/150‑1 (MQ=255)
                                                                                                                     aaataatACGGGAAGAGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  <  1:82988/150‑1 (MQ=255)
                                                                                                                        aaataCTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               <  2:287203/150‑1 (MQ=255)
                                                                                                                           aaccgagagggaaaGTTTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                             <  1:33696/139‑1 (MQ=255)
                                                                                                                                gAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                       >  1:701850/1‑151 (MQ=255)
                                                                                                                                gAAGAGAGGTTTGCGGGTATTGCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                        <  1:826197/150‑1 (MQ=255)
                                                                                                                                  gagagAAGGTTGCCGGTTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCATCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                       <  1:401553/146‑1 (MQ=255)
                                                                                                                                  aagaaaaaaTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                       <  2:585643/142‑1 (MQ=255)
                                                                                                                                          atggCCGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa              <  2:869920/148‑1 (MQ=255)
                                                                                                                                              cggggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          <  1:1097347/149‑1 (MQ=255)
                                                                                                                                              ccGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          <  2:968829/151‑1 (MQ=255)
                                                                                                                                              ccGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          <  1:983261/151‑1 (MQ=255)
                                                                                                                                              ccGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          <  2:1092846/151‑1 (MQ=255)
                                                                                                                                               cGGGATTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          <  1:281758/151‑1 (MQ=255)
                                                                                                                                               cGGGAATACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          <  1:1232244/151‑1 (MQ=255)
                                                                                                                                                    ttACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatac    <  1:583816/151‑1 (MQ=255)
                                                                                                                                                      aCCCCCGCCATATTCGCATTCGGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  <  2:1059835/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
ACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443131‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: