Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,6980TC55.3% 17.4 / 114.6 104D38G (GAT→GGT) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base C (0/57);  total (23/81)
Fisher's exact test for biased strand distribution p-value = 1.54e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-07

Only 100 of 105 total aligned reads displayed.

CTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTG  >  NC_000913/3665548‑3665842
                                                                                                                                                         |                                                                                                                                                 
ctacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCAt                                                                                                                                                   >  2:337795/1‑151 (MQ=255)
     gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCa                                                                                                                                              >  2:148397/1‑151 (MQ=255)
     gTGATGAACGAGTAATGAAGAAACTTTTTTATGCGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCa                                                                                                                                              >  1:113845/1‑151 (MQ=255)
           aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGCCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCaaaacca                                                                                                                                        <  1:1206365/151‑4 (MQ=255)
             cGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑Ag                                                                                                                                     >  1:136732/1‑151 (MQ=255)
              gAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑Agg                                                                                                                                    >  2:1432270/1‑151 (MQ=255)
                 tAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTc                                                                                                                                  <  1:927755/150‑1 (MQ=255)
                 tAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTc                                                                                                                                  <  1:46309/150‑1 (MQ=255)
                            cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTaaa                                                                                                                      >  2:1155325/1‑151 (MQ=255)
                             ttttttATGAGCATAAATATATATCGCCCCCCCCGGGAACATGGGGAAAAATAATAAAA‑GCGTGCCCCTAG‑ACGAAAATACAAAATTTTAAACACCTTAATCTACCCGAATTT‑GGCTTGCATCCGCAAAACCA‑AGGTCACCGCCATTaaaa                                                                                                                     <  1:1344085/151‑1 (MQ=255)
                                        cATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATAtt                                                                                                           >  2:772811/1‑150 (MQ=255)
                                        cATAAATATTTAATCCCTCCTCCTTGAGCATCGGGAAAAAAAATAAAA‑GCGTTCCCTTTG‑ACACAAATAAAAAATTTTCAACCCACTTATCTCCCCGATTTT‑GGCTTCCACCCGCAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTc                                                                                                          <  2:66550/151‑1 (MQ=255)
                                        aaTGAAGATTTAATCCCCCCTCCTTGAGCATGGGGAAAAAAAATAAAG‑GCGTTCCCTTTG‑ACACAAAAAAAAAATTTTCAACACCCTTATCTCCTCAATTTT‑GGCTTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTc                                                                                                          <  2:614638/150‑1 (MQ=255)
                                           aaaGTTTTAATGCCTCCTCCTGGACCATGGGAAAAAAAAATGAAG‑GCGTGTCCCTTG‑ACACGAATAAAAAATTTTAAACACTCTTATTTACTCGATTTT‑GGCTTCCACCCCAAAAACCC‑AGGTCTCCGCCATTAAAATAGCGATATTCAcc                                                                                                       <  1:590127/150‑1 (MQ=255)
                                            aataTTAAAGGCCCCCCCCTTAAGCATCGGGAAAAAAAATGAAA‑GCGTGCCCTTTG‑ACCCAAATAAAAAATTTCCAACACACTTATCTACCCAATTTT‑GGCTTCCATCCCAAAAACCC‑AGGTCCCCGCCATTAAAATAGCGATATTCAcc                                                                                                       <  2:812114/147‑1 (MQ=255)
                                                  tactcccccctcctTGAACTGCGGAAAAAATAAATAAA‑GCTTCTCCCTTG‑ACAAAAATACAAAATTTTCAACCCTCTTTTCTCCTCGATTTG‑GTCTTGCACCCGAAAAAACC‑AGCTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                <  1:402582/143‑1 (MQ=255)
                                                  taacgccccccactTGAGAATCGGAAAAAAAAATAAAG‑GCGTTTCCCTTG‑ACACAAATAAAAAATTTACACCCCCTTTATCTACTGAATTTT‑GGTTTCCACCCCAAAAAACA‑AGTTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                <  1:777078/139‑1 (MQ=255)
                                                  tAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAA‑GCGTGTCCTTTG‑ACACAAAAAAAAAATTTCCAACCCCCTTACCTACTAAATTTT‑GGCTTCCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                <  1:552416/151‑1 (MQ=255)
                                                  tAATGCCTCCTCCTGGACAATCGGAAGAAAAAATGAAG‑GCGTTTCCTTTG‑ACACGAATACAAAATTTTAAACACCTTTATCTACTAAAATTT‑GGCTTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                <  1:187038/151‑1 (MQ=255)
                                                  tAATGCCGCCTCCTGGAGCATCGGGAAAAAAAATAAAG‑GCGTGCCCTTTG‑ACGCAAATACAAAATTTTCAACCCACTTATATTCTCAATTTT‑GGCTTGCATCCGAAAAAACC‑GGGTACCCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                <  1:331661/151‑1 (MQ=255)
                                                  tAATCCCCCCTCTTTGGGCATCGGGAGAAATAATGAAA‑GTGGGCCCCTTG‑AAACAAATAAAAAATTTCAAACACACTTATCTACTCGATTTT‑GGCTTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                <  2:846977/151‑1 (MQ=255)
                                                  tAATCCCCCCTCCTGGAGCAGGGGGAAAAAAAATGAAA‑GCGTGTCCCTTG‑ACACAAAAAAAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCACCCAAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                <  2:131407/151‑1 (MQ=255)
                                                  aaaTCCCTCCTCCTTGACAATCGGAAAAAAAAATGAAC‑GTTTTCCCCTTA‑AAAAAAAAAAAAAATTTAAAACACCTTTATCTCCACAATTTT‑GGCTTCCACCCAAAAAAACC‑GGTCCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                <  1:1177801/150‑1 (MQ=255)
                                                   aaTGCCTCCTCATAATACATGGGAAAAAATAATAAAA‑GCTTGTCCCTTG‑ACACAAAACCAAAATTTTAAACACACTTCTCTACGCAATTTT‑GTCTTCCACCCACAAAACCC‑AGTTCTCCGCCATTAAAATAGCGATATTCACCATTAACCa                                                                                               <  1:1082223/151‑1 (MQ=255)
                                                     tGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                             >  1:610469/1‑151 (MQ=255)
                                                     tGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                             >  2:800859/1‑151 (MQ=255)
                                                       cccccccctTGAGCATGGGGAAAAAAAAAAAAA‑GCGTGTCCTTTG‑AAAAAAAAAAAAAATTTTCAACACACTTATCTCCTCAAATTT‑GGCTTGCATCCGAAAAAACC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                            <  2:1343379/144‑1 (MQ=255)
                                                          cctcctTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag                                                                                        >  1:78982/1‑151 (MQ=255)
                                                            tcctTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                      >  2:1315788/1‑151 (MQ=255)
                                                            tcctTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                      >  2:472187/1‑151 (MQ=255)
                                                             cctTGAGAATGGGAAAAAAAAATGAAA‑GCTTGTCCTTTG‑CCACAAAAACAAAATTTCCAACCCATTTATCTACTAAATTTT‑GGTTTCCACCCAAAAAACCC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                     <  1:557831/151‑1 (MQ=255)
                                                             cctTGACCAGGGGGAAAAAAAATAAAA‑GCGTGCCCCTTG‑ACACAAAAAAAAAATTTTCAACACCTTTATCTACTCGAATTG‑GGTTTGCACCCAAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                     <  2:895833/151‑1 (MQ=255)
                                                                  accttCGGGAAAAATAAGCACA‑GCATGTCCTGAT‑ACACGATTACAAATTTTACACCCCATTTATCTACACGATTTG‑GGCTTGCATCCGCAAAAACA‑GGTTAACCGCCATTAAAATAGCGATATTCAACATTAACCATGGTGAGAATATAtt                                                                                <  1:331826/147‑1 (MQ=255)
                                                                  aGCATCGGAAAAAATAATGAAA‑GCGTTCCCTTTG‑ACACGAATAAAAAATTTACAACACACTTACCTACTCAATTGG‑GGCTTGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                 <  1:1431540/150‑1 (MQ=255)
                                                                   gCATGGGGAAAAAAAATAAAA‑GCGTGTCCCTTG‑ACACGAATAAAAATTTTTCAACACACTTATCTACTCAAATTT‑GGCTTGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  2:1413064/150‑1 (MQ=255)
                                                                    catgggaaaaaaTAATGAAG‑GCGTGCCCTTTA‑CCACGAATAAAAAATTTAAACCACCCTTATCTTCCCAATTTT‑GGCTTCCACCCGCAAAACCC‑GGGGCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                              <  2:226698/142‑1 (MQ=255)
                                                                    catgggaaaaaaTAATAAAC‑GCGTCTCCCTGA‑ACACAAAAAAAAAATTTTCAACACAATTATCTACTCAATTTG‑GGTTTGCACCCGAAAAAACC‑AGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  1:912956/140‑1 (MQ=255)
                                                                    cagggggAAAAATAATAAAA‑GGGTGTCCCTTG‑ACACAAAAAAAAAATTTTCAACCCACTTATCTACTCGAATTT‑GGCTTGCACCCGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  2:309685/145‑1 (MQ=255)
                                                                    cagcgagaaaaaTAATGAAG‑GCTTGTCCTTTG‑AAACAAATACAAAATTTCCAACCCACTTACCTACTAAATTTT‑GGTTTACCCCCGAAAAACCC‑GGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  1:372435/140‑1 (MQ=255)
                                                                    cATGGGGAAAAAAAAGAAAA‑GCGTGTCCCTTG‑ACACAAATAAAAAATTTTCAACCCACTTATCTACTCGATTTT‑GGCTTGCACCCCAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  2:163567/149‑1 (MQ=255)
                                                                    cATCGGGAAAAATAATGAAA‑GCGTTTCCCTTG‑ACACGAATAAAAAATTTTAAACACACTTATCTCCTCGATTTT‑GGTTTGCACCCAAAAAAACA‑GGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  1:1032133/149‑1 (MQ=255)
                                                                    cATCGGGAAAAAAAAGGAAA‑GCGTGTCCCTTG‑ACACAAATACAAAATTTTCAACCCACTTATCTACTCAAATTT‑GGCTTGCACCCGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                               <  1:570208/150‑1 (MQ=255)
                                                                      tCGGGAAAAAAAAAGAAA‑GCGTGCCCTTTG‑ACACAAAAAAAAAATTTTCAACACATTTATCTCCTGGAATTT‑GGCTTCCACCCACAAAAACC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                            <  2:751713/151‑1 (MQ=255)
                                                                      cgggaaaaaaTAATAAAA‑GCGTCTCCCTTG‑ACACAAATAAAAAATTTCAACCACCCTTATCTACTAAATTTG‑GGTTTCCACCAGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                            <  1:718315/144‑1 (MQ=255)
                                                                       cggaaaaaaaaaTAAAA‑GCGTTTCCCTTG‑CCACAAATACAAATTTTTAAACACCCTTATCTCCCCAAATTT‑GGCTTGCACCCAAAAAACCC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                           <  2:683306/145‑1 (MQ=255)
                                                                       cGGGAAAAAAAAAAAAAGGCGTGTCCCTTG‑ACACGAATAAAAAATTTAAAACACCCTTATCTACCCAATTTT‑GGCTTCCACCCGCAAAAACA‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                            <  2:1420594/151‑1 (MQ=255)
                                                                        gagagaAGAAATGAAG‑GCTTGCCCCTGG‑ACAAGAATAAAAAATTGTCAACCTACTTATCTACCCAATTTT‑GGCTTCCACCCAAAAAAACG‑GGTCCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                           <  1:321054/148‑1 (MQ=255)
                                                                          gaaaaaaaaTAAAA‑GCGTGCCCCTTA‑ACACAAAAACAAAATTTACAACCCATTTATCTCCTCAAATTT‑GGTTTGCATCCCAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCt                                                                         <  2:963260/147‑1 (MQ=255)
                                                                                aaatGAAA‑GCTTTTCCATTG‑ACACGAATAAAAAATTTCCAACACACTTATCTTCCAAAATTT‑GGTTTGCACCCGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                  <  1:337692/150‑1 (MQ=255)
                                                                                       aaagcggccccTTGAACACAAAAAAAAAATTTACAACCCACTTATCTACGCGATTTG‑GGTTTACACCCGAAAAAACC‑AGGACCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                             <  2:841944/143‑1 (MQ=255)
                                                                                       a‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                           >  2:1387095/1‑151 (MQ=255)
                                                                                            tttCCTTTG‑ACACGAATAAAAAATTTTCAACACCCTTTTCTCATCAATTTT‑GCCTTGCATCCGCAAAAACC‑GGGTACCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                        <  1:904451/148‑1 (MQ=255)
                                                                                            tgccccTTG‑ACACGAAAAAAAAATTTTCAACACACTTATCTACTCAAATTT‑GGCTTGCACCCGAAAAAACC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                        <  2:162887/147‑1 (MQ=255)
                                                                                            tgccccTTG‑ACACAAATAAAAAATTTTCAACCCTTTTATCTACTCAATTTT‑GGCTTGCATCCGAAAAACCC‑GGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                        <  2:763045/147‑1 (MQ=255)
                                                                                             ttcccctgaacacAAAAAAAAAATTTAAAACACACTTATCTCCTCAAATTT‑GGTTTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                        <  2:738931/141‑1 (MQ=255)
                                                                                             ttCCCTTG‑ACACGAATAAAAAATTTTCACCACCCTTATCTACTCAAATTT‑GGCTTGCATCCAAAAAACCG‑AGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                      <  1:1434849/150‑1 (MQ=255)
                                                                                             tgtcctgtgaagcGAATAAAAAATTTTAAACAGCCTTATCTACCAAAATTT‑GGTTTGAATCCGAAAAAACC‑GGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                        <  1:1355855/138‑1 (MQ=255)
                                                                                               cccTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTTCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                    >  1:1366654/1‑151 (MQ=255)
                                                                                               cccTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                    >  2:1110676/1‑151 (MQ=255)
                                                                                                  tttaacacAAAAAAAAAATTTCCACCCCCTTTATCTACCCAAATTG‑GGCTTGCACCCAAAAAACCC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTcc                                                  <  2:610763/147‑1 (MQ=255)
                                                                                                      ccacaaaaaaaaaaTTTCAAACACACTTTTCTACAAAAATTT‑GTCTGGCCCCCGAAAAACCA‑AGTTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                              <  2:1500765/141‑1 (MQ=255)
                                                                                                      acacGAATAAAAATTTTTCAACCCCTTTATCTACTCAATTTT‑GGCTTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                              <  2:1123734/151‑1 (MQ=255)
                                                                                                      acaaGAAAAAAAAATTTCCAACCCCCTATCCTAC‑CAAATTTGGGCTGGCACCCGAAAAAACC‑ATTGCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                              <  2:1346085/151‑1 (MQ=255)
                                                                                                       cacaAATACAAAATTTTCAACACACTTATCCCCTCGAATTT‑GGCTTGCACCCACAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                             <  2:810021/151‑1 (MQ=255)
                                                                                                         caaaTAAAAATTTTTCAACCCATTTATCTCCTCAATTTT‑GGCTTGCACCCGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  2:707093/149‑1 (MQ=255)
                                                                                                         aaaacaaaaaaTTTACAACACACTTATCTCCTAGATTTT‑GTCTTGCATCCAAAAAACCC‑AGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  1:202526/144‑1 (MQ=255)
                                                                                                         aaaaaaaaaaaTTTTCAACACACTTATCTACTCAAATTT‑GGTTTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  2:570570/144‑1 (MQ=255)
                                                                                                           aaTACAAAATTTTCAACCCCCTTACCTACTAGATTTT‑GGCTTCCCCCCAAAAAAACA‑AGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                         <  2:760725/151‑1 (MQ=255)
                                                                                                           aaTAAAAAATTTAAAACACCTTTATCTACACAATTTT‑GGCTTGCACCCGAAAAAACC‑GGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                         <  1:1113465/151‑1 (MQ=255)
                                                                                                                  aattacaaaCACTCTAATCAACAGGAATTT‑GGCTGGCACCGAAAAAAACC‑AGGTCCCCGCCATTAAAATAGCGAGATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                  <  2:238471/144‑1 (MQ=255)
                                                                                                                     tttCACCACACTTATCCCCCCGATTTT‑GGCTTACACCCAAAAAAACC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                               <  2:468832/151‑1 (MQ=255)
                                                                                                                     tttAAACCCCCTTATCTACTAAATTTT‑GGCTGGCACCCGAAAAACCC‑AGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                               <  1:403358/151‑1 (MQ=255)
                                                                                                                       caaccacacTTATCTAATCGATTTT‑GTCTTCCTTCCACAAAACCC‑GGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                             <  1:124658/147‑1 (MQ=255)
                                                                                                                       acaccacacTTATCTACGCAATTTT‑GGTTTGCACCCAAAAAACCG‑GGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                             <  1:1315136/147‑1 (MQ=255)
                                                                                                                            ccccTTATCTCCTCAATTTT‑GGTTTCAACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                        <  1:869998/148‑1 (MQ=255)
                                                                                                                            ccccTTATCTACTCAAATTT‑GTCTTCCACCCGAAAAAACG‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                        <  1:1110150/148‑1 (MQ=255)
                                                                                                                            acatTTATCTACTCAAATTT‑GGCTTGCATCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                        <  2:551273/151‑1 (MQ=255)
                                                                                                                             cccTTATCTACTAAAATTT‑GGCTTGCATCCAAAAAAACC‑GAGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                       <  1:1103366/149‑1 (MQ=255)
                                                                                                                             cacTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                       >  2:247856/1‑151 (MQ=255)
                                                                                                                             cacTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                       >  2:537321/1‑151 (MQ=255)
                                                                                                                             cacTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                       >  1:704449/1‑151 (MQ=255)
                                                                                                                             cacTTATCTACTCGAATTT‑GGCATGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                       >  1:1230807/1‑151 (MQ=255)
                                                                                                                                ttttCTACTCAATTTT‑GTCTTGCACCAGAAAAAACC‑GGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  1:1372362/148‑1 (MQ=255)
                                                                                                                                ttATCTCCTCAATTTT‑GGCTTGCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:1167144/151‑1 (MQ=255)
                                                                                                                                ttATCTCCTAAATTTT‑GGTTTGCCCCCGAAAAACCC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:489090/151‑1 (MQ=255)
                                                                                                                                ttATCTCCTAAAATTT‑GGTTGGCACCCGAAAAAACC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  1:1428240/151‑1 (MQ=255)
                                                                                                                                ttATCTACTCAATTTT‑GGCTTGCATCCAAAAAAACC‑GGTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:40528/151‑1 (MQ=255)
                                                                                                                                     tactaaaaTTT‑GGCTTGCACCCCAAAAAACC‑GGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt               <  2:504169/145‑1 (MQ=255)
                                                                                                                                     tACTCGAATTT‑GGCTTGCATCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt               <  2:55692/151‑1 (MQ=255)
                                                                                                                                     tACTAGATTTG‑GGCTTCCA‑CCCCAAAAACCGAGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt               <  1:328045/151‑1 (MQ=255)
                                                                                                                                       cTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaa             >  1:1492429/1‑151 (MQ=255)
                                                                                                                                           atttt‑GGCTTCCACCCAAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat         <  1:995008/149‑1 (MQ=255)
                                                                                                                                           aaTTT‑GGTTTGCACCCGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat         <  2:1485461/151‑1 (MQ=255)
                                                                                                                                           aaTTT‑GGCTGGCCTCAGAAAAAACG‑AGTCCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat         <  1:254652/151‑1 (MQ=255)
                                                                                                                                                  gtttGCACCCAAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  1:191779/148‑1 (MQ=255)
                                                                                                                                                  gCTTGCACCCAAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  1:86695/150‑1 (MQ=255)
                                                                                                                                                  gCTTCCATCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  2:136776/150‑1 (MQ=255)
                                                                                                                                                  ccTTGCACCCGAAAAAACC‑AGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  2:1361255/149‑1 (MQ=255)
                                                                                                                                                   tttGCATCCGAAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg  <  1:148352/150‑1 (MQ=255)
                                                                                                                                                   cTTCCACCCGAAAAACCC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg  <  2:170980/151‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                                 
CTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCCTTG‑ACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTT‑GGCTTGCATCCGCAAAAACC‑AGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTG  >  NC_000913/3665548‑3665842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑