Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA63.5% 3.2 / 108.0 107A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (25/14);  new base A (0/68);  total (25/82)
Fisher's exact test for biased strand distribution p-value = 9.07e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-07

Only 100 of 108 total aligned reads displayed.

GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAT  >  NC_000913/3665553‑3665851
                                                                                                                                                       |                                                                                                                                                      
gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                                       >  2:148397/1‑151 (MQ=255)
gTGATGAACGAGTAATGAAGAAACTTTTTTATGCGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                                       >  1:113845/1‑151 (MQ=255)
      aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGCCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaacca                                                                                                                                                 <  1:1206365/151‑4 (MQ=255)
        cGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAg                                                                                                                                              >  1:136732/1‑151 (MQ=255)
        cGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAg                                                                                                                                              >  2:1390123/1‑151 (MQ=255)
         gAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAgg                                                                                                                                             >  2:1432270/1‑151 (MQ=255)
            tAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTc                                                                                                                                           <  1:46309/150‑1 (MQ=255)
            tAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTc                                                                                                                                           <  1:927755/150‑1 (MQ=255)
                      aCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaa                                                                                                                                >  1:1484678/1‑151 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  2:1155325/1‑151 (MQ=255)
                                   cATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                    >  2:772811/1‑150 (MQ=255)
                                   cATAAATATTTAATCCCTCCTCCTTGAGCATCGGGAAAAAAAATAAAAGCGTTCCCTTTGACACAAATAAAAAATTTTCAACCCACTTATCTCCCCGATTTTGGCTTCCACCCGCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTc                                                                                                                   <  2:66550/151‑1 (MQ=255)
                                   aaTGAAGATTTAATCCCCCCTCCTTGAGCATGGGGAAAAAAAATAAAGGCGTTCCCTTTGACACAAAAAAAAAATTTTCAACACCCTTATCTCCTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTc                                                                                                                   <  2:614638/150‑1 (MQ=255)
                                     tGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCa                                                                                                                  >  1:712723/1‑150 (MQ=255)
                                      aaaGTTTTAATGCCTCCTCCTGGACCATGGGAAAAAAAAATGAAGGCGTGTCCCTTGACACGAATAAAAAATTTTAAACACTCTTATTTACTCGATTTTGGCTTCCACCCCAAAAACCCAGGTCTCCGCCATTAAAATAGCGATATTCAcc                                                                                                                <  1:590127/150‑1 (MQ=255)
                                       aataTTAAAGGCCCCCCCCTTAAGCATCGGGAAAAAAAATGAAAGCGTGCCCTTTGACCCAAATAAAAAATTTCCAACACACTTATCTACCCAATTTTGGCTTCCATCCCAAAAACCCAGGTCCCCGCCATTAAAATAGCGATATTCAcc                                                                                                                <  2:812114/147‑1 (MQ=255)
                                             tactcccccctcctTGAACTGCGGAAAAAATAAATAAAGCTTCTCCCTTGACAAAAATACAAAATTTTCAACCCTCTTTTCTCCTCGATTTGGTCTTGCACCCGAAAAAACCAGCTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:402582/143‑1 (MQ=255)
                                             taacgccccccactTGAGAATCGGAAAAAAAAATAAAGGCGTTTCCCTTGACACAAATAAAAAATTTACACCCCCTTTATCTACTGAATTTTGGTTTCCACCCCAAAAAACAAGTTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:777078/139‑1 (MQ=255)
                                             tAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCTTTGACACAAAAAAAAAATTTCCAACCCCCTTACCTACTAAATTTTGGCTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:552416/151‑1 (MQ=255)
                                             tAATGCCTCCTCCTGGACAATCGGAAGAAAAAATGAAGGCGTTTCCTTTGACACGAATACAAAATTTTAAACACCTTTATCTACTAAAATTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:187038/151‑1 (MQ=255)
                                             tAATGCCGCCTCCTGGAGCATCGGGAAAAAAAATAAAGGCGTGCCCTTTGACGCAAATACAAAATTTTCAACCCACTTATATTCTCAATTTTGGCTTGCATCCGAAAAAACCGGGTACCCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:331661/151‑1 (MQ=255)
                                             tAATCCCCCCTCTTTGGGCATCGGGAGAAATAATGAAAGTGGGCCCCTTGAAACAAATAAAAAATTTCAAACACACTTATCTACTCGATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:846977/151‑1 (MQ=255)
                                             tAATCCCCCCTCCTGGAGCAGGGGGAAAAAAAATGAAAGCGTGTCCCTTGACACAAAAAAAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:131407/151‑1 (MQ=255)
                                             aaaTCCCTCCTCCTTGACAATCGGAAAAAAAAATGAACGTTTTCCCCTTAAAAAAAAAAAAAAATTTAAAACACCTTTATCTCCACAATTTTGGCTTCCACCCAAAAAAACCGGTCCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:1177801/150‑1 (MQ=255)
                                              aaTGCCTCCTCATAATACATGGGAAAAAATAATAAAAGCTTGTCCCTTGACACAAAACCAAAATTTTAAACACACTTCTCTACGCAATTTTGTCTTCCACCCACAAAACCCAGTTCTCCGCCATTAAAATAGCGATATTCACCATTAACCa                                                                                                        <  1:1082223/151‑1 (MQ=255)
                                                tGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                                      >  1:610469/1‑151 (MQ=255)
                                                tGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                                      >  2:800859/1‑151 (MQ=255)
                                                  cccccccctTGAGCATGGGGAAAAAAAAAAAAAGCGTGTCCTTTGAAAAAAAAAAAAAATTTTCAACACACTTATCTCCTCAAATTTGGCTTGCATCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                     <  2:1343379/144‑1 (MQ=255)
                                                    ccctcctTGAGCATCGGGAAAAAAAAGAAAAGCTTGCCCTTTGACACAAATAAAAAATTTTCACCCCCCTTTTCTACTCAAATTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                  <  2:540316/150‑1 (MQ=255)
                                                     cctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag                                                                                                 >  1:78982/1‑151 (MQ=255)
                                                       tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                               >  2:1315788/1‑151 (MQ=255)
                                                       tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                               >  2:472187/1‑151 (MQ=255)
                                                        cctTGAGAATGGGAAAAAAAAATGAAAGCTTGTCCTTTGCCACAAAAACAAAATTTCCAACCCATTTATCTACTAAATTTTGGTTTCCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                              <  1:557831/151‑1 (MQ=255)
                                                        cctTGACCAGGGGGAAAAAAAATAAAAGCGTGCCCCTTGACACAAAAAAAAAATTTTCAACACCTTTATCTACTCGAATTGGGTTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                              <  2:895833/151‑1 (MQ=255)
                                                             accttCGGGAAAAATAAGCACAGCATGTCCTGATACACGATTACAAATTTTACACCCCATTTATCTACACGATTTGGGCTTGCATCCGCAAAAACAGGTTAACCGCCATTAAAATAGCGATATTCAACATTAACCATGGTGAGAATATAtt                                                                                         <  1:331826/147‑1 (MQ=255)
                                                             aGCATCGGAAAAAATAATGAAAGCGTTCCCTTTGACACGAATAAAAAATTTACAACACACTTACCTACTCAATTGGGGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                          <  1:1431540/150‑1 (MQ=255)
                                                              gCATGGGGAAAAAAAATAAAAGCGTGTCCCTTGACACGAATAAAAATTTTTCAACACACTTATCTACTCAAATTTGGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:1413064/150‑1 (MQ=255)
                                                               catgggaaaaaaTAATGAAGGCGTGCCCTTTACCACGAATAAAAAATTTAAACCACCCTTATCTTCCCAATTTTGGCTTCCACCCGCAAAACCCGGGGCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                                       <  2:226698/142‑1 (MQ=255)
                                                               catgggaaaaaaTAATAAACGCGTCTCCCTGAACACAAAAAAAAAATTTTCAACACAATTATCTACTCAATTTGGGTTTGCACCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:912956/140‑1 (MQ=255)
                                                               cagggggAAAAATAATAAAAGGGTGTCCCTTGACACAAAAAAAAAATTTTCAACCCACTTATCTACTCGAATTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:309685/145‑1 (MQ=255)
                                                               cagcgagaaaaaTAATGAAGGCTTGTCCTTTGAAACAAATACAAAATTTCCAACCCACTTACCTACTAAATTTTGGTTTACCCCCGAAAAACCCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:372435/140‑1 (MQ=255)
                                                               cATGGGGAAAAAAAAGAAAAGCGTGTCCCTTGACACAAATAAAAAATTTTCAACCCACTTATCTACTCGATTTTGGCTTGCACCCCAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:163567/149‑1 (MQ=255)
                                                               cATCGGGAAAAATAATGAAAGCGTTTCCCTTGACACGAATAAAAAATTTTAAACACACTTATCTCCTCGATTTTGGTTTGCACCCAAAAAAACAGGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:1032133/149‑1 (MQ=255)
                                                                 tCGGGAAAAAAAAAGAAAGCGTGCCCTTTGACACAAAAAAAAAATTTTCAACACATTTATCTCCTGGAATTTGGCTTCCACCCACAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                     <  2:751713/151‑1 (MQ=255)
                                                                 cgggaaaaaaTAATAAAAGCGTCTCCCTTGACACAAATAAAAAATTTCAACCACCCTTATCTACTAAATTTGGGTTTCCACCAGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                     <  1:718315/144‑1 (MQ=255)
                                                                  cggaaaaaaaaaTAAAAGCGTTTCCCTTGCCACAAATACAAATTTTTAAACACCCTTATCTCCCCAAATTTGGCTTGCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  2:683306/145‑1 (MQ=255)
                                                                   gagagaAGAAATGAAGGCTTGCCCCTGGACAAGAATAAAAAATTGTCAACCTACTTATCTACCCAATTTTGGCTTCCACCCAAAAAAACGGGTCCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  1:321054/148‑1 (MQ=255)
                                                                     gaaaaaaaaTAAAAGCGTGCCCCTTAACACAAAAACAAAATTTACAACCCATTTATCTCCTCAAATTTGGTTTGCATCCCAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCt                                                                                  <  2:963260/147‑1 (MQ=255)
                                                                           aaatGAAAGCTTTTCCATTGACACGAATAAAAAATTTCCAACACACTTATCTTCCAAAATTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                           <  1:337692/150‑1 (MQ=255)
                                                                                 aaagcggccccTTGAACACAAAAAAAAAATTTACAACCCACTTATCTACGCGATTTGGGTTTACACCCGAAAAAACCAGGACCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                      <  2:841944/143‑1 (MQ=255)
                                                                                  aGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                                    >  2:1387095/1‑151 (MQ=255)
                                                                                      tttCCTTTGACACGAATAAAAAATTTTCAACACCCTTTTCTCATCAATTTTGCCTTGCATCCGCAAAAACCGGGTACCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  1:904451/148‑1 (MQ=255)
                                                                                      tgccccTTGACACGAAAAAAAAATTTTCAACACACTTATCTACTCAAATTTGGCTTGCACCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  2:162887/147‑1 (MQ=255)
                                                                                      tgccccTTGACACAAATAAAAAATTTTCAACCCTTTTATCTACTCAATTTTGGCTTGCATCCGAAAAACCCGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  2:763045/147‑1 (MQ=255)
                                                                                       ttcccctgaacacAAAAAAAAAATTTAAAACACACTTATCTCCTCAAATTTGGTTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  2:738931/141‑1 (MQ=255)
                                                                                       ttCCCTTGACACGAATAAAAAATTTTCACCACCCTTATCTACTCAAATTTGGCTTGCATCCAAAAAACCGAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                               <  1:1434849/150‑1 (MQ=255)
                                                                                       tgtcctgtgaagcGAATAAAAAATTTTAAACAGCCTTATCTACCAAAATTTGGTTTGAATCCGAAAAAACCGGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  1:1355855/138‑1 (MQ=255)
                                                                                         cccTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTTCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                             >  1:1366654/1‑151 (MQ=255)
                                                                                         cccTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                             >  2:1110676/1‑151 (MQ=255)
                                                                                            tttaacacAAAAAAAAAATTTCCACCCCCTTTATCTACCCAAATTGGGCTTGCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTcc                                                           <  2:610763/147‑1 (MQ=255)
                                                                                                ccacaaaaaaaaaaTTTCAAACACACTTTTCTACAAAAATTTGTCTGGCCCCCGAAAAACCAAGTTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                       <  2:1500765/141‑1 (MQ=255)
                                                                                                acacGAATAAAAATTTTTCAACCCCTTTATCTACTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                       <  2:1123734/151‑1 (MQ=255)
                                                                                                acaaGAAAAAAAAATTTCCAACCCCCTATCCTACCAAATTTGGGCTGGCACCCGAAAAAACCATTGCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                       <  2:1346085/151‑1 (MQ=255)
                                                                                                 cacaAATACAAAATTTTCAACACACTTATCCCCTCGAATTTGGCTTGCACCCACAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                      <  2:810021/151‑1 (MQ=255)
                                                                                                   aaaacaaaaaaTTTACAACACACTTATCTCCTAGATTTTGTCTTGCATCCAAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  1:202526/144‑1 (MQ=255)
                                                                                                   aaaaaaaaaaaTTTTCAACACACTTATCTACTCAAATTTGGTTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  2:570570/144‑1 (MQ=255)
                                                                                                     aaTACAAAATTTTCAACCCCCTTACCTACTAGATTTTGGCTTCCCCCCAAAAAAACAAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                                  <  2:760725/151‑1 (MQ=255)
                                                                                                     aaTAAAAAATTTAAAACACCTTTATCTACACAATTTTGGCTTGCACCCGAAAAAACCGGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                                  <  1:1113465/151‑1 (MQ=255)
                                                                                                            aattacaaaCACTCTAATCAACAGGAATTTGGCTGGCACCGAAAAAAACCAGGTCCCCGCCATTAAAATAGCGAGATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                           <  2:238471/144‑1 (MQ=255)
                                                                                                               tttCACCACACTTATCCCCCCGATTTTGGCTTACACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                        <  2:468832/151‑1 (MQ=255)
                                                                                                               tttAAACCCCCTTATCTACTAAATTTTGGCTGGCACCCGAAAAACCCAGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                        <  1:403358/151‑1 (MQ=255)
                                                                                                                 caaccacacTTATCTAATCGATTTTGTCTTCCTTCCACAAAACCCGGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                                      <  1:124658/147‑1 (MQ=255)
                                                                                                                 acaccacacTTATCTACGCAATTTTGGTTTGCACCCAAAAAACCGGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                                      <  1:1315136/147‑1 (MQ=255)
                                                                                                                      ccccTTATCTCCTCAATTTTGGTTTCAACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                                 <  1:869998/148‑1 (MQ=255)
                                                                                                                      ccccTTATCTACTCAAATTTGTCTTCCACCCGAAAAAACGAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                                 <  1:1110150/148‑1 (MQ=255)
                                                                                                                      acatTTATCTACTCAAATTTGGCTTGCATCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                                 <  2:551273/151‑1 (MQ=255)
                                                                                                                       cccTTATCTACTAAAATTTGGCTTGCATCCAAAAAAACCGAGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                <  1:1103366/149‑1 (MQ=255)
                                                                                                                       cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  1:704449/1‑151 (MQ=255)
                                                                                                                       cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  2:537321/1‑151 (MQ=255)
                                                                                                                       cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  2:247856/1‑151 (MQ=255)
                                                                                                                       cacTTATCTACTCGAATTTGGCATGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  1:1230807/1‑151 (MQ=255)
                                                                                                                          ttttCTACTCAATTTTGTCTTGCACCAGAAAAAACCGGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  1:1372362/148‑1 (MQ=255)
                                                                                                                          ttATCTCCTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  2:1167144/151‑1 (MQ=255)
                                                                                                                          ttATCTCCTAAATTTTGGTTTGCCCCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  2:489090/151‑1 (MQ=255)
                                                                                                                          ttATCTCCTAAAATTTGGTTGGCACCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  1:1428240/151‑1 (MQ=255)
                                                                                                                               tactaaaaTTTGGCTTGCACCCCAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                        <  2:504169/145‑1 (MQ=255)
                                                                                                                               tACTCGAATTTGGCTTGCATCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                        <  2:55692/151‑1 (MQ=255)
                                                                                                                               tACTAGATTTGGGCTTCCACCCCAAAAACCGAGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                        <  1:328045/151‑1 (MQ=255)
                                                                                                                                 cTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaa                      >  1:1492429/1‑151 (MQ=255)
                                                                                                                                     aaTTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                  <  2:1485461/151‑1 (MQ=255)
                                                                                                                                     aaTTTGGCTGGCCTCAGAAAAAACGAGTCCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                  <  1:254652/151‑1 (MQ=255)
                                                                                                                                            gCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  1:86695/150‑1 (MQ=255)
                                                                                                                                            gCTTCCATCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  2:136776/150‑1 (MQ=255)
                                                                                                                                            ccTTGCACCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  2:1361255/149‑1 (MQ=255)
                                                                                                                                             tttGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg           <  1:148352/150‑1 (MQ=255)
                                                                                                                                             cTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg           <  2:170980/151‑1 (MQ=255)
                                                                                                                                                ccacccGAAAAAACCAGTTCTCCTCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACGTAATATTACATAAACATATTACTGttt        <  1:1488675/147‑1 (MQ=255)
                                                                                                                                                     cacAAAACCAAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTaa   <  1:1223129/149‑1 (MQ=255)
                                                                                                                                                     cGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTaa   >  1:1264949/1‑151 (MQ=255)
                                                                                                                                                      gCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAt  >  2:158090/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAT  >  NC_000913/3665553‑3665851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: