Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,458,804	A→T	I181F (ATC→TTC)	paaG →	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,458,804	0	A	T	100.0%	288.1 / NA	76	I181F (ATC→TTC)	paaG	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (37/39);  total (37/39)

ATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGATCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCAACTGGAT  >  NC_000913/1458656‑1458950                                                                                                                                                     |                                                                                                                                                   aTTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTc                                                                                                                                                  <  2:1282583/151‑1 (MQ=255) aTTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTc                                                                                                                                                  <  2:171318/151‑1 (MQ=255)      tCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGc                                                                                                                                              <  1:398234/150‑1 (MQ=255)      tCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCa                                                                                                                                             <  1:58284/151‑1 (MQ=255)        aTGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAgg                                                                                                                                           <  2:229652/151‑1 (MQ=255)           gCGTTTGGCAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGTGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGAGGGCTGTTCTGGCAGgttg                                                                                                                                        >  1:1234664/1‑151 (MQ=255)             gTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGgttgtt                                                                                                                                      >  2:444068/1‑151 (MQ=255)                      gTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGaa                                                                                                                             >  2:1032828/1‑151 (MQ=255)                      gTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGaa                                                                                                                             >  2:1220456/1‑151 (MQ=255)                           gcctAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACg                                                                                                                          >  1:1105476/4‑149 (MQ=255)                           gCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGc                                                                                                                         <  2:26123/150‑1 (MQ=255)                              tAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTgg                                                                                                                      >  2:168265/1‑150 (MQ=255)                              tAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTgg                                                                                                                      >  2:1043924/1‑150 (MQ=255)                              tAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTgg                                                                                                                      >  2:70275/1‑150 (MQ=255)                                aTACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGCACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAg                                                                                                                   <  1:57855/151‑1 (MQ=255)                                aTACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAg                                                                                                                   >  2:1345065/1‑151 (MQ=255)                                aTACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAg                                                                                                                   <  1:1368610/151‑1 (MQ=255)                                    ccGATTGCGGTGGAGCCTGGTTACTGCCACGCGTGGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATAc                                                                                                               <  1:949015/151‑1 (MQ=255)                                    ccGATTGCGGTGGAACCTGGTTGCTGGCACGCGGTGCCGGACGAGCGCGCGCCATGGGGCGGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGAGGATGAAACGCTGGCAGATAc                                                                                                               <  1:1490336/151‑1 (MQ=255)                                    ccGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATAc                                                                                                               <  1:355010/151‑1 (MQ=255)                                    ccGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATAc                                                                                                               <  2:627761/151‑1 (MQ=255)                                    ccGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATAc                                                                                                               <  2:913275/151‑1 (MQ=255)                                     cGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATAc                                                                                                               >  1:480616/1‑150 (MQ=255)                                         tGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCgcgc                                                                                                          <  2:1197045/151‑1 (MQ=255)                                                 aaCCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAg                                                                                                     >  2:1386557/1‑148 (MQ=255)                                                      ggTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACg                                                                                             >  2:1401902/1‑151 (MQ=255)                                                      ggTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACg                                                                                             >  1:8844/1‑151 (MQ=255)                                                          aCTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCAt                                                                                         <  1:737234/151‑1 (MQ=255)                                                          aCTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCAt                                                                                         <  1:1105283/151‑1 (MQ=255)                                                               cACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGc                                                                                    <  1:679477/151‑1 (MQ=255)                                                                     ttGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGAcaca                                                                              <  2:766219/151‑1 (MQ=255)                                                                     ttGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGAcaca                                                                              <  2:998635/151‑1 (MQ=255)                                                                     ttGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGAcaca                                                                              >  1:1119246/1‑151 (MQ=255)                                                                               aGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACAtt                                                                     <  2:589612/150‑1 (MQ=255)                                                                                   cgcgcCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGt                                                                 <  1:314422/150‑1 (MQ=255)                                                                                   cgcgcCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGt                                                                 <  1:414298/150‑1 (MQ=255)                                                                                   cgcgcCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGt                                                                 <  1:443913/150‑1 (MQ=255)                                                                                       ccATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACCCAACCGACATTTGGTCTTgg                                                            >  1:536639/1‑151 (MQ=255)                                                                                       ccATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTgg                                                            >  1:909450/1‑151 (MQ=255)                                                                                       ccATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTgg                                                            >  2:1490802/1‑151 (MQ=255)                                                                                         aTGGGGCTGGGACTGCTGGGTAATCAGCTGATAGGTGACCAGGCGCACGAATGGGGGATGTTGTGGCAGGTTTTTGATGAGGAAGCGGTGGCAGATATCGCGCACCAGCTGGCACGGCATCTGGCGACTCGGCCGACGTTTGGTTTTGGag                                                          <  1:261145/151‑2 (MQ=255)                                                                                         aTGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACGGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGAc                                                          <  2:555444/151‑1 (MQ=255)                                                                                         aTGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACCCAACCGACATTTGGTCTTGGAc                                                          <  1:291093/151‑1 (MQ=255)                                                                                         aTGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGAc                                                          <  2:152212/151‑1 (MQ=255)                                                                                         aTGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGAc                                                          <  2:1453249/151‑1 (MQ=255)                                                                                         aTGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGAc                                                          <  2:1353905/151‑1 (MQ=255)                                                                                         aTGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGAc                                                          <  2:1290220/151‑1 (MQ=255)                                                                                         aTGGGGCTGGCACTGCTGGGGAAGCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCCCGGCATCTGGCGACACAACCGACATTTGGTCTTGGAc                                                          <  1:911947/151‑1 (MQ=255)                                                                                            gggCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTa                                                       >  2:881685/1‑151 (MQ=255)                                                                                            gggCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTa                                                       >  2:723583/1‑151 (MQ=255)                                                                                            gggCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTa                                                       >  2:1024884/1‑151 (MQ=255)                                                                                            gggCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTa                                                       >  1:361246/1‑151 (MQ=255)                                                                                                     ctgctgGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATcaagcaag                                              >  2:713303/1‑151 (MQ=255)                                                                                                            ggAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATaaa                                       >  2:154317/1‑151 (MQ=255)                                                                                                             gAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAAt                                      >  1:943949/1‑151 (MQ=255)                                                                                                              aaTCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAAt                                      <  2:1064017/150‑1 (MQ=255)                                                                                                              aaTCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGAACGGCATCTGGCGACACAACCGACATGTGGTCTTGGACTTATCAAGCAAGCGATAAAt                                      <  1:970121/150‑1 (MQ=255)                                                                                                               aTCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAg                                    <  2:8858/151‑1 (MQ=255)                                                                                                                  aaCTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTTGTCTTGGACTTATCAAGCAAGCGATAAATAgcgc                                 <  1:349137/151‑1 (MQ=255)                                                                                                                   aCTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTGTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATAAAGCAAGCGAAAAATAgcg                                  >  2:122/1‑149 (MQ=255)                                                                                                                   aCTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAgcg                                  >  2:162092/1‑149 (MQ=255)                                                                                                                   aCTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAgcg                                  >  1:1386045/1‑149 (MQ=255)                                                                                                                   aCTGAGTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAgcg                                  >  2:274657/1‑149 (MQ=255)                                                                                                                        gTGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGaaa                            >  2:1262642/1‑150 (MQ=255)                                                                                                                         tGCTGAACAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAAcc                          <  2:1253432/151‑1 (MQ=255)                                                                                                                              aaCAGGCGCACGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATAc                     <  1:1454527/151‑1 (MQ=255)                                                                                                                                       aCGAATGGGGGATGTTCTGGCAGGTTGTTTATGATGAAACGCTGGCAAATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATAc            <  1:528489/151‑1 (MQ=255)                                                                                                                                        cGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACg           >  1:86036/1‑151 (MQ=255)                                                                                                                                        cGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACg           >  2:737803/1‑151 (MQ=255)                                                                                                                                        cGAATGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACg           >  2:673949/1‑151 (MQ=255)                                                                                                                                          aaTGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCa         >  2:878252/1‑151 (MQ=255)                                                                                                                                          aaTGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCa         >  2:1047088/1‑151 (MQ=255)                                                                                                                                          aaTGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCa         >  1:870212/1‑151 (MQ=255)                                                                                                                                           aTGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCaa        >  1:275270/1‑151 (MQ=255)                                                                                                                                            tGGGGGATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCAAc       <  2:1268498/151‑1 (MQ=255)                                                                                                                                                ggATGTTCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCAACTGGa   >  1:882989/1‑151 (MQ=255)                                                                                                                                                  atgttCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAATCCAATACGCTCGATACGCAACTGGAt  <  2:1349772/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGGCACTGCTGGGGAATCAACTGAGTGCTGAACAGGCGCACGAATGGGGGATGATCTGGCAGGTTGTTGATGATGAAACGCTGGCAGATACCGCGCAACAGCTGGCACGGCATCTGGCGACACAACCGACATTTGGTCTTGGACTTATCAAGCAAGCGATAAATAGCGCTGAAACCAATACGCTCGATACGCAACTGGAT  >  NC_000913/1458656‑1458950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑