Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,896,268 T→A A33A (GCT→GCA nudL → putative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,896,2680TA98.4% 235.0 / ‑5.4 63A33A (GCT→GCAnudLputative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (30/32);  minor base G (0/1);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCG  >  NC_000913/1896122‑1896414
                                                                                                                                                  |                                                                                                                                                  
aCTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAGCCCTAAATCATCGTCAGGCTGCAgtgt                                                                                                                                                <  1:1215621/151‑1 (MQ=255)
aCTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAgtgt                                                                                                                                                <  2:93517/151‑1 (MQ=255)
 cTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGtt                                                                                                                                               >  2:16864/1‑151 (MQ=255)
 cTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGtt                                                                                                                                               >  1:609757/1‑151 (MQ=255)
  ttttGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTa                                                                                                                                              <  1:806214/151‑1 (MQ=255)
  ttttGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTa                                                                                                                                              <  1:63034/151‑1 (MQ=255)
     tGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATc                                                                                                                                           <  2:130480/151‑1 (MQ=255)
     tGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATc                                                                                                                                           <  2:3133/151‑1 (MQ=255)
     tGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATc                                                                                                                                           <  1:824961/151‑1 (MQ=255)
       aTAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATccc                                                                                                                                         <  2:364315/151‑1 (MQ=255)
       aTAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATcc                                                                                                                                          <  2:818448/150‑1 (MQ=255)
       aTAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATcc                                                                                                                                          <  2:1039858/150‑1 (MQ=255)
       aTAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATcc                                                                                                                                          <  1:1011509/150‑1 (MQ=255)
       aTAAAGTTAATCGTATCCTGAAGCAACTAGAGAAGTAAGACGAGGAATACCATAGCCTGACGGTCGATGATGTTCTATCGCGCTTCCAACGTCTGCGACCGCAAATTAACCGGGAAACCCGAAGTAATCGTCAAGCTGCGGTGttcaacca                                                                                                                                         <  1:1406696/151‑7 (MQ=255)
           aGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCAt                                                                                                                                      >  1:836271/1‑150 (MQ=255)
            gTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATcg                                                                                                                                    >  1:1460331/1‑151 (MQ=255)
             ttAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATcg                                                                                                                                    <  2:630618/150‑1 (MQ=255)
                aTCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATcgtccg                                                                                                                                >  1:56024/1‑151 (MQ=255)
                aTCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATcgtccg                                                                                                                                >  1:96988/1‑151 (MQ=255)
                     aTCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGa                                                                                                                            >  2:401364/1‑150 (MQ=255)
                     aTCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGa                                                                                                                            >  2:600227/1‑150 (MQ=255)
                           aaGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAAc                                                                                                                     <  2:527945/151‑1 (MQ=255)
                              cAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAAcc                                                                                                                    <  2:1297773/149‑1 (MQ=255)
                               aaCTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCggg                                                                                                                 >  1:877938/1‑151 (MQ=255)
                                aCTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCgggg                                                                                                                >  1:937172/1‑151 (MQ=255)
                                 cTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGgt                                                                                                               <  2:200265/151‑1 (MQ=255)
                                 cTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGgt                                                                                                               >  1:1308719/1‑151 (MQ=255)
                                       aaGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGttgttg                                                                                                          >  2:952089/1‑150 (MQ=255)
                                           aaGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGAc                                                                                                     <  2:263460/151‑1 (MQ=255)
                                              aCGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTc                                                                                                   <  1:832226/150‑1 (MQ=255)
                                              aCGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCa                                                                                                  >  2:1206939/1‑151 (MQ=255)
                                               cGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGAATCAg                                                                                                 >  1:1289442/1‑151 (MQ=255)
                                                gTGGAATCCCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAg                                                                                                 >  1:969371/1‑150 (MQ=255)
                                                gTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAg                                                                                                 >  1:22880/1‑150 (MQ=255)
                                                gTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAg                                                                                                 >  1:546873/1‑150 (MQ=255)
                                                       aCCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGAt                                                                                         >  1:1196055/1‑151 (MQ=255)
                                                        ccGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGAtt                                                                                        >  1:480755/1‑151 (MQ=255)
                                                                      ttGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAaca                                                                          <  1:1368746/151‑1 (MQ=255)
                                                                      ttGATGAGTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAaca                                                                          <  1:747098/151‑1 (MQ=255)
                                                                              tttttATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTTATCTGCGTAAACACGCTGGa                                                                   >  2:750041/1‑150 (MQ=255)
                                                                               ttttATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACa                                                                 <  1:980396/151‑1 (MQ=255)
                                                                                 ttATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAg                                                               <  1:823484/151‑1 (MQ=255)
                                                                                  tATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGt                                                              <  2:979831/151‑1 (MQ=255)
                                                                                         cTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTc                                                       <  2:1075459/151‑1 (MQ=255)
                                                                                                 ttttGCGCCCGCAAATTAACCGGGAAACCCTAATTCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCAACCGCAGCCGGGGTTGTTGCTGACTCAGCGTTCTATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAgg                                               <  1:599938/151‑1 (MQ=255)
                                                                                                 ttttGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAgg                                               <  2:150840/151‑1 (MQ=255)
                                                                                                      cgcCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAg                                          <  1:1322190/151‑1 (MQ=255)
                                                                                                            cAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATg                                    >  2:1367486/1‑151 (MQ=255)
                                                                                                              aaTTAACCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGac                                  <  2:587118/151‑1 (MQ=255)
                                                                                                                  aaCCGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCAGCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACgg                              <  1:526990/151‑1 (MQ=255)
                                                                                                                     cGGGAAACCCTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACg                           <  2:322382/151‑1 (MQ=255)
                                                                                                                            cccTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAg                     >  2:731735/1‑150 (MQ=255)
                                                                                                                            cccTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAg                     >  2:526593/1‑150 (MQ=255)
                                                                                                                            cccTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAg                     >  1:850254/1‑150 (MQ=255)
                                                                                                                            cccTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAg                     >  2:1254443/1‑150 (MQ=255)
                                                                                                                              cTAAATCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTa                  <  2:1047899/151‑1 (MQ=255)
                                                                                                                                aaaTCATCGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTAt                 >  2:1180723/1‑150 (MQ=255)
                                                                                                                                  atcatcGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATcg               >  2:1457596/1‑150 (MQ=255)
                                                                                                                                   tcatcGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATcgcc             <  2:262097/151‑1 (MQ=255)
                                                                                                                                      tcGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGTGGTGCAGTCGATGACACGGACGCATCAGCTATcgccgc           >  1:5411/1‑150 (MQ=255)
                                                                                                                                      tcGTCAGGCTGCAGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATcgccgc           >  2:283199/1‑150 (MQ=255)
                                                                                                                                          cgggcggcAGTGTGAATCCCCATCGTCCGTCGACCGCAACCGGGGTTCTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCgcgc      <  1:66865/149‑1 (MQ=255)
                                                                                                                                            ggCTGCAGCGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCcgcg       >  2:37705/1‑148 (MQ=255)
                                                                                                                                             gctgcaGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTAGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCgcgc      <  1:491291/148‑1 (MQ=255)
                                                                                                                                              ctgcagTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTgcg  >  1:156775/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTTTTGATAAAGTTAATCGTATCCTGAAGCAACTGGAGAAGTAAGACGTGGAATACCGTAGCCTGACGCTTGATGATTTTTTATCGCGCTTTCAACTTTTGCGCCCGCAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCG  >  NC_000913/1896122‑1896414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: