Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,752,089 A→T S99C (AGC→TGC)  recN → recombination and repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,752,0890AT97.2% 277.3 / NA 72S99C (AGC→TGC) recNrecombination and repair protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (44/26);  minor base C (2/0);  total (46/26)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCACCAAACCTGAGCACCAAAAATT  >  NC_000913/2751943‑2752225
                                                                                                                                                  |                                                                                                                                        
cTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCg                                                                                                                                       <  1:540621/150‑1 (MQ=255)
  gAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATg                                                                                                                                    >  1:492706/1‑151 (MQ=255)
  gAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATg                                                                                                                                    >  1:823591/1‑151 (MQ=255)
        gACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCg                                                                                                                                >  2:766118/1‑149 (MQ=255)
        gACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCg                                                                                                                                >  2:1244412/1‑149 (MQ=255)
             ggTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGt                                                                                                                         >  1:872430/1‑151 (MQ=255)
             ggTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGt                                                                                                                         >  2:459292/1‑151 (MQ=255)
              gTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGGAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTg                                                                                                                        >  1:541233/1‑151 (MQ=255)
              gTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTg                                                                                                                        >  1:45161/1‑151 (MQ=255)
              gTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTg                                                                                                                        >  2:1057804/1‑151 (MQ=255)
              gTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTg                                                                                                                        >  2:1288217/1‑151 (MQ=255)
              gTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTg                                                                                                                        >  2:1301573/1‑151 (MQ=255)
                   tACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGttt                                                                                                                    >  2:1340825/1‑150 (MQ=255)
                        gcgcTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCaa                                                                                                              >  2:135586/1‑151 (MQ=255)
                        gcgcTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCaa                                                                                                              >  2:1401633/1‑151 (MQ=255)
                              ctCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTAc                                                                                                        <  1:572198/151‑1 (MQ=255)
                                 gtgcTGACCTGTGCGACCTGTTTGCTCTGAAAGTTACGCAAGCGCCCTTGCGCTGGCTGGTAGAAAATCAGCCTGAAGACGCGCATGATTGTTTGCTTCGGCGCGAGATCCGCCGCGATGGTCGCGTCCGTGGTTTCATCAACGGTatcg                                                                                                      >  1:774730/3‑147 (MQ=255)
                                  cgcTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAg                                                                                                      >  1:367600/1‑149 (MQ=255)
                                    cTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTg                                                                                                   <  2:1207981/150‑1 (MQ=255)
                                    cTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTg                                                                                                   <  1:96471/150‑1 (MQ=255)
                                    cTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTg                                                                                                   <  2:1005436/150‑1 (MQ=255)
                                    cTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTg                                                                                                   <  2:1321118/150‑1 (MQ=255)
                                      gACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGtt                                                                                                 >  2:31565/1‑150 (MQ=255)
                                      gACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGtt                                                                                                 >  2:1508041/1‑150 (MQ=255)
                                      gACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGtt                                                                                                 >  2:402148/1‑150 (MQ=255)
                                      gACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGtt                                                                                                 >  2:535840/1‑150 (MQ=255)
                                      gACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGtt                                                                                                 >  2:1328731/1‑150 (MQ=255)
                                      gACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGtt                                                                                                 >  1:1286717/1‑150 (MQ=255)
                                           gtgCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCct                                                                                              >  1:347809/1‑148 (MQ=255)
                                                cccGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTcaca                                                                                      >  2:526979/1‑151 (MQ=255)
                                                  cGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAAc                                                                                    <  2:1485009/151‑1 (MQ=255)
                                                  cGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAAc                                                                                    <  1:1209918/151‑1 (MQ=255)
                                                  cGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAAc                                                                                    <  1:183145/151‑1 (MQ=255)
                                                   gTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACaa                                                                                     >  1:232569/1‑149 (MQ=255)
                                                    tttttCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTg                                                                                  <  1:674618/151‑1 (MQ=255)
                                                           ctGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGaa                                                                            >  2:1047418/1‑150 (MQ=255)
                                                             gAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGaa                                                                            >  2:1408260/1‑148 (MQ=255)
                                                                aGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGt                                                                      >  1:739579/1‑151 (MQ=255)
                                                                aGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGt                                                                      >  1:84017/1‑151 (MQ=255)
                                                                aGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGt                                                                      >  2:384058/1‑151 (MQ=255)
                                                                  aTACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTc                                                                     <  2:1452525/150‑1 (MQ=255)
                                                                   tACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAg                                                                   <  1:932397/151‑1 (MQ=255)
                                                                    aCGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAg                                                                   <  1:977186/150‑1 (MQ=255)
                                                                       ccAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTg                                                                <  1:622125/150‑1 (MQ=255)
                                                                       ccAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGc                                                               <  1:1266609/151‑1 (MQ=255)
                                                                        cAGCGGCTCTGCGTTGGCTGGAAGAAAACAAGCATGAAGACGGACATGAAGGTTTTCATCGTCGCGTGAACAACCACGAATGACGCACCCGAGGTTTCAGCAACGGCACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGGCAGTTggt                                                              >  1:607785/1‑149 (MQ=255)
                                                                             gCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGAtt                                                          >  2:757348/1‑150 (MQ=255)
                                                                              ctctGCGCTGGCTGGAAGAAAACCAGCGTGAAGACGGGCATGAATGTTTGCTGCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTGCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTc                                                         <  1:206106/150‑1 (MQ=255)
                                                                                  gcgcTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGAt                                                     >  1:1437919/1‑150 (MQ=255)
                                                                                  gcgcTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGAt                                                     >  2:1360595/1‑150 (MQ=255)
                                                                                  gcgcTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGAt                                                     <  1:981419/150‑1 (MQ=255)
                                                                                       ggctggAAGAAAACCAGCTTGAAGACGGGCCTGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCCGTCACAACTGCGCGAACTGGGTCAGTGGCTGAGTCAGATCCATgg                                               >  1:1328655/1‑151 (MQ=255)
                                                                                       ggctggAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATgg                                               >  1:739224/1‑151 (MQ=255)
                                                                                            gaagaaAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAg                                           >  1:204812/1‑150 (MQ=255)
                                                                                            gaagaaAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAg                                           >  2:933588/1‑150 (MQ=255)
                                                                                                  aaCCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGAACAGCTGTTCCTCTGTCAAAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCt                                     >  2:317117/1‑150 (MQ=255)
                                                                                                       gcatGAAGACGCGCATGAATGCTGGCTTCGCCTCGTGATCAGCTGCCATGGACGCTCCCGTGGATTCACCCACCGTACAGCTGTTCCTCTGTCACAACTGCCCGAACTGGTACAGTTGCTGATTCAGATCCCTGGTCAGCACGCTCATCaa                               >  1:871853/4‑151 (MQ=255)
                                                                                                        cTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCtcatc                                 <  2:961144/148‑1 (MQ=255)
                                                                                                        cTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAAt                              >  2:1020830/1‑151 (MQ=255)
                                                                                                        cTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAAt                              <  1:436184/151‑1 (MQ=255)
                                                                                                          tGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTa                            <  1:1435454/151‑1 (MQ=255)
                                                                                                           gAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTa                            >  1:922978/1‑150 (MQ=255)
                                                                                                            aaGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTAc                           <  1:440356/150‑1 (MQ=255)
                                                                                                            aaGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTGCCTCTGTCACAGCTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTAc                           <  1:41195/150‑1 (MQ=255)
                                                                                                               aCGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCAc                       <  1:765672/151‑1 (MQ=255)
                                                                                                               aCGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCAc                       <  1:140454/151‑1 (MQ=255)
                                                                                                                 gggCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCACCa                     >  2:299243/1‑151 (MQ=255)
                                                                                                                  ggCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGCACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCAccca                    >  1:340780/1‑149 (MQ=255)
                                                                                                                  ggCATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCACCaa                    >  1:470415/1‑151 (MQ=255)
                                                                                                                    cATGAATGTTTGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCCCCaaa                   >  2:280171/1‑150 (MQ=255)
                                                                                                                            tttGCTTCGTCGCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCACCAAACCTGAGCAc          <  1:436811/151‑1 (MQ=255)
                                                                                                                                    ctcgCGTGATCAGCTGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCACCAAACCTGAGCACCAAAAAtt  <  1:5212/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                        
CTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAGCGATGGTCGCTCCCGTGGTTTCATCAACGGTACAGCTGTTCCTCTGTCACAACTGCGCGAACTGGGTCAGTTGCTGATTCAGATCCATGGTCAGCACGCTCATCAATTACTCACCAAACCTGAGCACCAAAAATT  >  NC_000913/2751943‑2752225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑