Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,924,915 A→T V240E (GTG→GAG)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,924,9150AT98.4% 478.4 / NA 127V240E (GTG→GAG) mnmG5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (70/54);  minor base C (2/0);  total (72/55)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGC  >  NC_000913/3924768‑3925058
                                                                                                                                                   |                                                                                                                                               
tCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTcc                                                                                                                                               <  1:1009348/150‑1 (MQ=255)
tCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTcc                                                                                                                                               <  1:1415446/150‑1 (MQ=255)
        gcgcATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGa                                                                                                                                      >  1:1035936/1‑151 (MQ=255)
        gcgcATGACTTTGTCTTCGATCGACGGGCACTAGCGTGGGCCGACACCTTCCATCACCCCTGCGTACATTGGGCTACGACCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGa                                                                                                                                      >  1:322469/1‑151 (MQ=255)
         cgcATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTgggg                                                                                                                                       <  2:288673/149‑1 (MQ=255)
         cgcATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGAt                                                                                                                                     <  2:510198/151‑1 (MQ=255)
               aCTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTggg                                                                                                                               >  2:96556/1‑151 (MQ=255)
               aCTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTggg                                                                                                                               >  1:1209495/1‑151 (MQ=255)
               aCTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTggg                                                                                                                               >  1:63889/1‑151 (MQ=255)
                 tttGTCTTCGATCGACTGGCAGGTGCGTGGGCCGACACCTTCGATCATACGAGAGTTCATTGGGCTAGGCTCGATGTTACTGCGGATCACATCATGGTTTTTGTTGTTGATATGAGTTATATAACACGGCGGctgctgatgttgctaatac                                                                                                                             <  1:1270469/151‑14 (MQ=255)
                  ttGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACCCCTTCGATCGCCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACg                                                                                                                            <  2:259124/151‑1 (MQ=255)
                               ccGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCACGGGTTTTCCCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATAGCCCATaaa                                                                                                               <  1:1033448/150‑1 (MQ=255)
                               aCGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATaaa                                                                                                               <  2:170855/151‑1 (MQ=255)
                               aCGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATaaa                                                                                                               <  1:112012/151‑1 (MQ=255)
                                  ggCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACg                                                                                                             <  1:196208/150‑1 (MQ=255)
                                      gTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAAt                                                                                                        >  2:634971/1‑151 (MQ=255)
                                        aGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATa                                                                                                       >  1:647779/1‑150 (MQ=255)
                                         gCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGCTACTGCGGATCACATCATGGGTTTTATCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATAc                                                                                                      >  1:958482/1‑150 (MQ=255)
                                                   accctgaggATCACGACTGCTTACATTGGGATGCGATAGATGTTGCTGCCGATCACGTCATGCGTTTTCACGATGGTATGAGTGATAAAACACGGCTCCTGCTGGGGATGCTGGGGAGCCTTGCCCATAAACGAGAATACCGGCATTggg                                                                                            <  1:18685/142‑1 (MQ=255)
                                                   acacCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGt                                                                                           <  2:187664/151‑1 (MQ=255)
                                                      ccTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTAt                                                                                        >  2:1213625/1‑151 (MQ=255)
                                                        ttCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCg                                                                                      <  1:603389/151‑1 (MQ=255)
                                                        ttCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCg                                                                                      <  1:1208439/151‑1 (MQ=255)
                                                        ttCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCg                                                                                      <  1:654750/151‑1 (MQ=255)
                                                        ttCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCg                                                                                      >  1:913426/1‑151 (MQ=255)
                                                         tCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCg                                                                                      >  2:186898/1‑150 (MQ=255)
                                                            aTCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCa                                                                                   <  2:49412/150‑1 (MQ=255)
                                                              cgccccTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGc                                                                                <  1:1457942/149‑1 (MQ=255)
                                                              cACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGc                                                                                <  2:314288/151‑1 (MQ=255)
                                                              cACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGc                                                                                <  2:395410/151‑1 (MQ=255)
                                                              cACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGc                                                                                <  2:940847/151‑1 (MQ=255)
                                                               aCCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATCCCGGCATTGGGTTATCGCCATGCt                                                                               <  1:1000808/151‑1 (MQ=255)
                                                                ccccTGCGTACATTGGGCTACGATCGAGGCTACTGCGGATAACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTTCGACGCATTGCCAATAAACGAGAATACCGGCACTGGGTTATCGCCATg                                                                                 >  1:1185274/1‑148 (MQ=255)
                                                                 cccTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGt                                                                             <  1:715580/151‑1 (MQ=255)
                                                                  ccTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGc                                                                                     >  1:1313932/1‑142 (MQ=255)
                                                                  ccTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGc                                                                                     <  2:1314585/142‑1 (MQ=255)
                                                                   cTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTg                                                                           <  2:468441/151‑1 (MQ=255)
                                                                   cTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTg                                                                           <  1:1355542/151‑1 (MQ=255)
                                                                    tGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTg                                                                           >  2:734182/1‑150 (MQ=255)
                                                                     gCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTgcg                                                                         >  2:774084/1‑151 (MQ=255)
                                                                              gggCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACg                                                                 >  2:1204219/1‑150 (MQ=255)
                                                                              gggCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACg                                                                 >  2:896939/1‑150 (MQ=255)
                                                                              gggCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACg                                                                 >  1:652925/1‑150 (MQ=255)
                                                                               ggCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACg                                                                 >  2:1482051/1‑149 (MQ=255)
                                                                               ggCTACGATCGAGGTTACTGCGGATAACATCATGGGTTTTCTAGTTGGTATGAGTGATATAACACGGCCCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACAGGCATTGGGTTATCGCCATGATGTTGCGCCAGTACg                                                                 >  1:531393/1‑149 (MQ=255)
                                                                                    cGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGt                                                          <  1:1079784/151‑1 (MQ=255)
                                                                                    cGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGt                                                          >  2:417029/1‑151 (MQ=255)
                                                                                    cGATCGAGGTTACTGCGGATCACATCATGGGTTGTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGt                                                          <  1:750276/151‑1 (MQ=255)
                                                                                      aTCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCg                                                        >  2:1177556/1‑151 (MQ=255)
                                                                                        cGAGGTTACTGCGGATCACATCGTGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGAt                                                      <  2:274059/151‑1 (MQ=255)
                                                                                        cGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCa                                                                                                 <  2:1338130/108‑1 (MQ=255)
                                                                                        cGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCa                                                                                                 >  1:1337472/1‑108 (MQ=255)
                                                                                                   cGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCa                                            <  1:862789/150‑1 (MQ=255)
                                                                                                    ggATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGc                                                                                >  1:1453242/1‑113 (MQ=255)
                                                                                                    ggATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGc                                                                                <  2:1453982/113‑1 (MQ=255)
                                                                                                         acaTCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATAc                                     <  1:114934/151‑1 (MQ=255)
                                                                                                          cgtcatGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAAAACCGGCATGGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATAcg                                    <  1:592936/149‑1 (MQ=255)
                                                                                                          catcatGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATAcg                                    >  1:1314583/1‑151 (MQ=255)
                                                                                                          catcatGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATAcg                                    >  2:408476/1‑151 (MQ=255)
                                                                                                          catcatGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATAcg                                    >  2:1069911/1‑151 (MQ=255)
                                                                                                               tGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCggtg                               >  1:1250967/1‑151 (MQ=255)
                                                                                                                gggTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCggtgg                              >  2:605908/1‑151 (MQ=255)
                                                                                                                 ggTTTTCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCggtgg                              <  2:783171/150‑1 (MQ=255)
                                                                                                                     ttCTCGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTcc                         <  2:1036444/151‑1 (MQ=255)
                                                                                                                        tcGTTGGTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCgg                      <  1:96520/151‑1 (MQ=255)
                                                                                                                             ggTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGtttt                  >  2:1360168/1‑150 (MQ=255)
                                                                                                                             ggTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGtttt                  >  1:320046/1‑150 (MQ=255)
                                                                                                                             ggTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGtttt                  >  2:116175/1‑150 (MQ=255)
                                                                                                                             ggTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGtttt                  >  2:1463077/1‑150 (MQ=255)
                                                                                                                             ggTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCAATGCTGTTGCGCCAGCACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGGGTCCCGGtttt                  >  1:761416/1‑150 (MQ=255)
                                                                                                                              gTATGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCa                >  1:833431/1‑151 (MQ=255)
                                                                                                                                aTGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAga              <  2:849887/151‑1 (MQ=255)
                                                                                                                                aTGAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAga              <  1:1430576/151‑1 (MQ=255)
                                                                                                                                  gAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCCTGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGCGGTGTCCCGGTTTTCAgacg            >  2:1047191/1‑151 (MQ=255)
                                                                                                                                  gAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAgacg            >  1:225302/1‑151 (MQ=255)
                                                                                                                                  gAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAgac             <  1:976703/150‑1 (MQ=255)
                                                                                                                                  gAGTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAgac             >  2:977191/1‑150 (MQ=255)
                                                                                                                                    gTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAgacga           >  2:1073666/1‑150 (MQ=255)
                                                                                                                                    gTGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAgacga           >  1:1413109/1‑150 (MQ=255)
                                                                                                                                     tGATATAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAgacgac          >  1:918814/1‑150 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCCTCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:695145/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  2:750152/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:1220224/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:1287295/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:1046054/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:1302823/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:1042063/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:1488103/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:396839/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:458196/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:535644/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  2:1493141/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:574734/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       <  2:1413778/151‑1 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  2:1210498/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:776087/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCaa       >  1:876885/1‑151 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGCGTCCCGGTTTCCCGACGACCa        >  1:233440/1‑150 (MQ=255)
                                                                                                                                       atatAACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAAAGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCa        >  1:977050/1‑150 (MQ=255)
                                                                                                                                          taACACGGCTCCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAAcg     >  2:416132/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TCGGCGAAGCGCATGACTTTGTCTTCGATCGACGGGCAGTAGCGTGGGCCGACACCTTCGATCACCCCTGCGTACATTGGGCTACGATCGAGGTTACTGCGGATCACATCATGGGTTTTCTCGTTGGTATGAGTGATATAACACGGCACCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGC  >  NC_000913/3924768‑3925058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑