Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,310,950 T→A I11F (ATC→TTC)  alsA ← D‑allose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,310,9500TA99.0% 384.1 / NA 104I11F (ATC→TTC) alsAD‑allose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (31/72);  minor base G (0/1);  total (31/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCAT  >  NC_000913/4310803‑4311097
                                                                                                                                                   |                                                                                                                                                   
cTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAAccc                                                                                                                                                  <  1:1025753/151‑1 (MQ=255)
      tCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCAt                                                                                                                                            <  1:329658/151‑1 (MQ=255)
          cTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGat                                                                                                                                        <  1:352295/151‑1 (MQ=255)
              tGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatat                                                                                                                                    <  2:1170794/151‑1 (MQ=255)
              tGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatat                                                                                                                                    <  2:412268/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCCCCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  2:185595/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  2:1128458/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  2:1128299/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  2:1103533/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  1:723948/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  2:245100/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  1:474658/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  1:185936/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  2:539675/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  1:1285273/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  1:126326/151‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatata                                                                                                                                   <  1:1398959/150‑1 (MQ=255)
                tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTCCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGatatatat                                                                                                                                  <  1:1489848/151‑1 (MQ=255)
                 aTTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATg                                                                                                                                 <  2:1286366/151‑1 (MQ=255)
                  ttCCGGATAAAGCTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATgg                                                                                                                                <  1:392529/151‑1 (MQ=255)
                   tCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGc                                                                                                                               <  1:675174/151‑1 (MQ=255)
                   tCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGc                                                                                                                               <  2:1366993/151‑1 (MQ=255)
                    ccGGATAAAACTTTCATTAGCGTGTATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCAACTTTAATGCGTGAACCGGACCAAAGGACTTTCCGAACCCCGCCATCGATATATATGGCg                                                                                                                              <  1:1397355/151‑1 (MQ=255)
                    ccGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCg                                                                                                                              <  2:1330239/151‑1 (MQ=255)
                    ccGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCg                                                                                                                              <  1:287597/151‑1 (MQ=255)
                      ggATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTg                                                                                                                            >  1:1096029/1‑151 (MQ=255)
                             aCTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCataa                                                                                                                     >  2:1302264/1‑151 (MQ=255)
                               tttCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCataaat                                                                                                                   <  2:302332/151‑1 (MQ=255)
                                       gCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACctct                                                                                                            >  1:936582/1‑150 (MQ=255)
                                          tGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGc                                                                                                        <  1:938509/151‑1 (MQ=255)
                                           ggATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCa                                                                                                       >  2:453882/1‑151 (MQ=255)
                                           ggATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCa                                                                                                       >  2:77310/1‑151 (MQ=255)
                                            gATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCa                                                                                                       >  1:1438183/1‑150 (MQ=255)
                                            gATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCa                                                                                                       >  2:107245/1‑150 (MQ=255)
                                                 aCCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAat                                                                                                 <  2:332041/151‑1 (MQ=255)
                                                 aCCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAat                                                                                                 <  1:107993/151‑1 (MQ=255)
                                                 aCCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAat                                                                                                 <  1:435889/151‑1 (MQ=255)
                                                      cgcCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGt                                                                                            <  2:1438914/151‑1 (MQ=255)
                                                      cgcCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGt                                                                                            <  1:492368/151‑1 (MQ=255)
                                                        ccATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGtat                                                                                          >  1:1029011/1‑151 (MQ=255)
                                                        ccATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGtat                                                                                          <  1:140191/151‑1 (MQ=255)
                                                          aTTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGtatta                                                                                        <  1:1367210/151‑1 (MQ=255)
                                                              tctcCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTg                                                                                     >  1:1400747/1‑150 (MQ=255)
                                                               ctcCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACTTCTCTGCACGAAATATCGTATTATTGcc                                                                                   <  2:467660/151‑1 (MQ=255)
                                                               ctcCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGcc                                                                                   <  1:517179/151‑1 (MQ=255)
                                                               ctcCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGcc                                                                                   <  1:579837/151‑1 (MQ=255)
                                                                tcCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGc                                                                                    >  2:1038512/1‑149 (MQ=255)
                                                                  cTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGa                                                                                >  2:121018/1‑151 (MQ=255)
                                                                   tAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGAt                                                                               >  1:426150/1‑151 (MQ=255)
                                                                    aGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATg                                                                              >  2:511098/1‑151 (MQ=255)
                                                                          gCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACa                                                                        >  2:400167/1‑151 (MQ=255)
                                                                           cATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACAt                                                                       <  1:989314/151‑1 (MQ=255)
                                                                              gTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTa                                                                    >  1:111925/1‑151 (MQ=255)
                                                                              gTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTa                                                                    >  2:651688/1‑151 (MQ=255)
                                                                                aTTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCTGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATc                                                                  <  1:490640/151‑1 (MQ=255)
                                                                                aTTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATc                                                                  <  1:93683/151‑1 (MQ=255)
                                                                                aTTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATc                                                                  <  1:77275/151‑1 (MQ=255)
                                                                                  ttCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAg                                                                <  2:1497882/151‑1 (MQ=255)
                                                                                   tCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAgg                                                               >  2:331201/1‑151 (MQ=255)
                                                                                    cACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGt                                                              >  1:251367/1‑151 (MQ=255)
                                                                                      ccAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGt                                                              >  1:200801/1‑149 (MQ=255)
                                                                                      ccAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGt                                                              >  1:1454374/1‑149 (MQ=255)
                                                                                        aGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTa                                                           >  2:1018221/1‑150 (MQ=255)
                                                                                         ggATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACg                                                         >  2:1476790/1‑151 (MQ=255)
                                                                                         ggATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACg                                                         >  1:796473/1‑151 (MQ=255)
                                                                                           aTAGACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAGCCCCGCCATCGATATATATGGCGTGGCCATAAATATCCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGt                                                        <  1:875886/150‑1 (MQ=255)
                                                                                           aTAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGt                                                        <  1:945173/150‑1 (MQ=255)
                                                                                           aTAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGt                                                        <  2:622264/150‑1 (MQ=255)
                                                                                           aTAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGt                                                        <  2:1320428/150‑1 (MQ=255)
                                                                                           aTAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGt                                                        <  2:556255/150‑1 (MQ=255)
                                                                                               aCCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAgg                                                   <  1:922593/151‑1 (MQ=255)
                                                                                               aCCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAgg                                                   <  2:60054/151‑1 (MQ=255)
                                                                                               aCCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAgg                                                   <  1:116365/151‑1 (MQ=255)
                                                                                                 cGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGtt                                                 <  1:1077282/151‑1 (MQ=255)
                                                                                                 cGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGtt                                                 <  1:510901/151‑1 (MQ=255)
                                                                                                       aTTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTttg                                           <  2:1272336/151‑1 (MQ=255)
                                                                                                               aCTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCgg                                   >  1:1081864/1‑151 (MQ=255)
                                                                                                               aCTTTAATGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCgg                                   >  2:461591/1‑151 (MQ=255)
                                                                                                                      tGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGac                            <  1:47798/151‑1 (MQ=255)
                                                                                                                      tGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGac                            <  1:639761/151‑1 (MQ=255)
                                                                                                                      tGCGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGac                            <  1:107207/151‑1 (MQ=255)
                                                                                                                        cGTGAACCGGACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGacac                          <  2:269625/151‑1 (MQ=255)
                                                                                                                                 gACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAg                  <  1:1140814/150‑1 (MQ=255)
                                                                                                                                 gACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAg                  <  1:331102/150‑1 (MQ=255)
                                                                                                                                 gACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAg                  <  1:422535/150‑1 (MQ=255)
                                                                                                                                 gACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAg                  <  1:537816/150‑1 (MQ=255)
                                                                                                                                 gACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAg                  <  2:1301238/150‑1 (MQ=255)
                                                                                                                                 gACCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAg                  <  2:1050985/150‑1 (MQ=255)
                                                                                                                                  aCCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGca                <  1:30881/151‑1 (MQ=255)
                                                                                                                                  aCCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGca                <  1:143071/151‑1 (MQ=255)
                                                                                                                                  aCCAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGc                 <  1:737570/150‑1 (MQ=255)
                                                                                                                                   ccAAAGGACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcat               >  2:885588/1‑151 (MQ=255)
                                                                                                                                        ggACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTTGCCGGATAAGACACTTCATTAGcatcatca          >  2:1294312/1‑151 (MQ=255)
                                                                                                                                         gACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcatcat         >  1:327866/1‑151 (MQ=255)
                                                                                                                                         gACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcatca          <  1:825087/150‑1 (MQ=255)
                                                                                                                                         gACTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcatca          <  1:91476/150‑1 (MQ=255)
                                                                                                                                          aCTTGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcatcat         <  2:1442535/150‑1 (MQ=255)
                                                                                                                                             tGCCGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCgg     >  1:1223218/1‑151 (MQ=255)
                                                                                                                                                cGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGcat  <  2:169445/151‑1 (MQ=255)
                                                                                                                                                cGAACCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGcat  <  2:658514/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCAT  >  NC_000913/4310803‑4311097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑