Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	720,946	C→T	intergenic (+106/+110)	ybfK → / ← kdpE	uncharacterized protein/response regulator in two‑component regulatory system with KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	720,946	0	C	T	100.0%	133.3 / NA	35	intergenic (+106/+110)	ybfK/kdpE	uncharacterized protein/response regulator in two‑component regulatory system with KdpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/30);  total (5/30)

CGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAA  >  NC_000913/720806‑721093                                                                                                                                             |                                                                                                                                                    cGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATaa                                                                                                                                           <  1:1069278/151‑1 (MQ=255)    cTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAat                                                                                                                                        <  1:1506341/151‑1 (MQ=255)      tttATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGTAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAatat                                                                                                                                      <  1:438421/150‑1 (MQ=255)           tttCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAAt                                                                                                                                 <  2:1240382/151‑1 (MQ=255)                      tttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtg                                                                                                                      <  1:950706/151‑1 (MQ=255)                             ttCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGtgttg                                                                                                               >  1:321694/1‑151 (MQ=255)                                      tgaatgaaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGAt                                                                                                       <  1:995787/150‑1 (MQ=255)                                      tgaatgaaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGAt                                                                                                       <  1:564469/150‑1 (MQ=255)                                      tgaatgaaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGAt                                                                                                       <  1:1235788/150‑1 (MQ=255)                                            aaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGc                                                                                                <  2:407923/151‑1 (MQ=255)                                            aaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTCACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGc                                                                                                <  2:1091872/151‑1 (MQ=255)                                                 gAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtat                                                                                           >  1:334676/1‑151 (MQ=255)                                                       atTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttatta                                                                                     <  1:1331460/151‑1 (MQ=255)                                                        ttCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttattat                                                                                    <  2:1116689/151‑1 (MQ=255)                                                              ccAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGc                                                                              <  1:1211414/151‑1 (MQ=255)                                                                  gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCt                                                                             <  2:983474/148‑1 (MQ=255)                                                                          gTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGATGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaatt                                                                       <  2:306661/146‑1 (MQ=255)                                                                          gTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGATGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaatt                                                                       >  1:306575/1‑146 (MQ=255)                                                                           ttGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATtaataa                                                                  <  2:1305484/150‑1 (MQ=255)                                                                                  gtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggcagg                                                          >  1:1429541/1‑151 (MQ=255)                                                                                   tgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggcaggc                                                         <  2:443774/151‑1 (MQ=255)                                                                                   tgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggcaggc                                                         <  2:262653/151‑1 (MQ=255)                                                                                         tGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTAtt                                                   <  2:334775/151‑1 (MQ=255)                                                                                             cAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTTCGGCTGTAATTAATAAAAGGCAGGCTGGATTaaaa                                               <  1:1081010/151‑1 (MQ=255)                                                                                              aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAAt                                              <  2:1090297/151‑1 (MQ=255)                                                                                                                  aaTTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAcc                          <  2:1456366/151‑1 (MQ=255)                                                                                                                          taATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCa                  <  2:321786/151‑1 (MQ=255)                                                                                                                               atgaGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATAcc             <  2:366336/151‑1 (MQ=255)                                                                                                                               atgaGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATAc              <  1:38897/150‑1 (MQ=255)                                                                                                                                    cTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGtt         <  1:1212810/150‑1 (MQ=255)                                                                                                                                    cTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGtt         <  1:993336/150‑1 (MQ=255)                                                                                                                                        ttACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGt    <  2:1479550/151‑1 (MQ=255)                                                                                                                                          aCTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTaa  <  2:121361/151‑1 (MQ=255)                                                                                                                                          aCTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTaa  <  2:983425/151‑1 (MQ=255)                                                                                                                                          aCTTAGTAATATAAAATATTGAATTGTTATTTTTGTGCGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTaa  >  1:907209/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                    CGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAA  >  NC_000913/720806‑721093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑