New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 843349 =37 (0.730)6 (0.120) 5/282 5.0 14.9% coding (2132/2226 nt) ybiO mechanosensitive channel protein, intermediate conductance
?NC_000913 843377 = 33 (0.690)coding (2104/2226 nt) ybiO mechanosensitive channel protein, intermediate conductance
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

CCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCAGCCCGCGAATTTCTTCGTTTTCCATTAAT  >  NC_000913/843231‑843525
                                                                                                                                                  |                                                                                                                                                    
ccgccgTTTTCTGAATCGCCAGCGTTAATGCGTTGTTTCCCCCGGCGGTAACGGTTGAGCAGGCGTCGCGGCCGCGGCAGGCCGCACCTGATAAGTCTGCGCTGGGGCCCGAACGCCCGCCAGGTCGAAATTTTTTTTGAGCTGGCTGtgg                                                                                                                                                  <  1:73096/151‑3 (MQ=255)
  gccgTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACCGTTCAGCCGGGGTCCCGCCCGGAGCAGGCAGCACCTGATAAGGCTGCACTGGCGCAGGACCTCCAGCCAGGTCGTAATGCTTGTTCACCGGGCTGTCgcg                                                                                                                                                >  1:460776/1‑149 (MQ=255)
  cccgTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAg                                                                                                                                                <  1:503952/150‑1 (MQ=255)
    cgTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCCGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAggg                                                                                                                                              >  1:658502/1‑151 (MQ=255)
               tCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTAc                                                                                                                                    >  2:4984/1‑150 (MQ=255)
                     gCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCa                                                                                                                              >  2:794386/1‑150 (MQ=255)
                     gCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCa                                                                                                                              >  2:722569/1‑150 (MQ=255)
                     gCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCa                                                                                                                              >  2:237252/1‑150 (MQ=255)
                      cGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCAc                                                                                                                             <  1:32111/150‑1 (MQ=255)
                              cGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAg                                                                                                                     <  1:668297/150‑1 (MQ=255)
                                ttGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTg                                                                                                                   <  2:400900/150‑1 (MQ=255)
                                              ggTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACg                                                                                                    >  1:363292/1‑151 (MQ=255)
                                               gTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGa                                                                                                   >  1:9914/1‑151 (MQ=255)
                                                  aCGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAac                                                                                                <  2:482376/151‑1 (MQ=255)
                                                      ttCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGc                                                                                            <  1:147509/151‑1 (MQ=255)
                                                                 tCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGGGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAAcg                                                                                  <  1:809542/150‑1 (MQ=255)
                                                                 gcgcgCCCGGAGCAGGCAGCACCGGATAAGTCTGCACTGGCGCGAGAACCAGCACCAGGTCGAAATGTTTTTTCACCTGGGTGTCGAGGGCAAAGCGTGCCTTCCTCTGTTGGAGGGGCAGCGTGGTGAAAGAGACACTCAGTGTAAAcg                                                                                  <  1:87596/149‑1 (MQ=255)
                                                                      cccGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTAt                                                                            <  2:449782/151‑1 (MQ=255)
                                                                           aGCAGGCAGCACCTAATAAGTCTACACTGGCGCGCGAACGCCCGCCAGTTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAATCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCTGTATTGCt                                                                        <  1:205522/150‑1 (MQ=255)
                                                                            gcaggcagCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCtt                                                                       <  1:294061/150‑1 (MQ=255)
                                                                             caggcagCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCtt                                                                       <  1:167960/149‑1 (MQ=255)
                                                                                 cagcaCCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGcc                                                                  <  1:89006/150‑1 (MQ=255)
                                                                                      ccTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGGCGAGGGAAAAGCGTACCGTCCAc                                                                                                                             >  2:769282/1‑86 (MQ=255)
                                                                                             aGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCa                                                     >  1:36319/1‑151 (MQ=255)
                                                                                              gTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCaa                                                    >  1:318360/1‑151 (MQ=255)
                                                                                                cTGCACTGGCGCGCGAAAGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCaaaa                                                  >  1:468731/1‑151 (MQ=255)
                                                                                                      cgctttgccctcgaccgccAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGACGTCAATCCCGGCAAAATTCGGt                                            >  1:143934/15‑151 (MQ=255)
                                                                                                       ggCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGtt                                           >  1:512286/1‑151 (MQ=255)
                                                                                                           cgcgAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCAcc                                       <  2:381381/151‑1 (MQ=255)
                                                                                                             cgAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCaa                                     <  2:618882/151‑1 (MQ=255)
                                                                                                                   acagccAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAACTTCGGTTCACCAATAATCa                               >  1:106993/4‑151 (MQ=255)
                                                                                                                   acagccAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCa                               >  1:434415/4‑151 (MQ=255)
                                                                                                                    ccgccAGGTCGAAATGTTTTTTCACCTGGCTGTGGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCAg                              <  1:305397/151‑1 (MQ=255)
                                                                                                                                   gTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCAGCCCGCGAATttctt                >  2:329808/1‑150 (MQ=255)
                                                                                                                                   gTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCAGCCCGCGAATttctt                >  2:345541/1‑150 (MQ=255)
                                                                                                                                     tttttCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCAGCCCGCGAATTTCTTCGt             >  1:296424/1‑151 (MQ=255)
                                                                                                                                                 cTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCAGCCCGCGAATTTCTTCGTTTTCCATTAAt  <  1:805046/150‑1 (MQ=255)
                                                                                                                                                 cTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCAGCCCGCGAATTTCTTCGTTTTCCATTAAt  <  1:222511/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTTTTTCACCTGGCTGTCGAGGGCAAAGCGTACCGTCCACTGTTTGAGTGGCAGCGTGGTGAACGAAACACGCAGTGTAAACGCGGTATTGCTTAAGCCGACAATCCCGGCAAAATTCGGTTCACCAATAATCAGCCCGCGAATTTCTTCGTTTTCCATTAAT  >  NC_000913/843231‑843525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.