Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,2870GC51.3% 27.7 / 53.5 37intergenic (+93/+136)yhdZ/rrfFputative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base G (3/15);  new base C (19/0);  total (22/15)
Fisher's exact test for biased strand distribution p-value = 8.71e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-05

TCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATG  >  NC_000913/3423146‑3423425
                                                                                                                                              |                                                                                                                                            
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGGCCTTcccccccccccc                                                                                                                                      >  2:92004/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGGCCCGcccccccccccc                                                                                                                                      >  1:63393/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGcccccccccccc                                                                                                                                      >  2:557162/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGcccccccccccc                                                                                                                                      >  2:215684/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGCCCGcccccccc                                                                                                                                      >  2:403790/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTCcccccccccccc                                                                                                                                      >  2:210034/1‑148 (MQ=255)
  cTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCCCTCcccccccccccccg                                                                                                                                   >  2:572896/1‑149 (MQ=255)
    aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGc                                                                                                                                 <  1:134030/151‑1 (MQ=255)
    aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTTCCCCCCCCCCCCCggg                                                                                                                                  >  2:496781/1‑150 (MQ=255)
          gagCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCCGGCCGttt                                                                                                                            >  1:464048/1‑150 (MQ=37)
            gCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCGGTGttt                                                                                                                         >  2:758514/1‑151 (MQ=255)
              gTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTCCCCCCCCCCCCCCGGGCGTTGCTTc                                                                                                                        >  2:180741/1‑150 (MQ=37)
               tACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCCCGCccccccccccccccggcccttgtttg                                                                                                                       >  1:779514/1‑136 (MQ=255)
               tACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCGTTGCtttg                                                                                                                       >  2:477231/1‑148 (MQ=255)
                   aGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCACCCCCCCCGCCGCTGCTTTTCAAc                                                                                                                  >  1:756537/1‑150 (MQ=255)
                    gggCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCCGCCGTTgtctctcaca                                                                                                                   >  1:86280/1‑140 (MQ=255)
                          ttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTTCCCCCCCCCCTCCCGGCCCGTGCTTCTCAACGTTCAAAt                                                                                                          >  1:587777/1‑151 (MQ=25)
                           tttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATc                                                                                                         <  2:393774/151‑1 (MQ=255)
                           tttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATc                                                                                                         <  2:792122/151‑1 (MQ=255)
                            ttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATcc                                                                                                        <  2:602174/151‑1 (MQ=255)
                                     gTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCCCCCGGCCGGTGCTTTGCAACCTTTCAATcccccccccg                                                                                                >  2:408849/1‑148 (MQ=21)
                                          ccATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAtt                                                                                          <  1:27425/151‑1 (MQ=255)
                                          ccATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGCTGCTTTGCCAAGTTTAAAATCGCCCCccgcggggt                                                                                          >  2:772102/1‑148 (MQ=18)
                                               aaTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTcc                                                                                     <  2:647097/151‑1 (MQ=255)
                                                aTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATTGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCCCCCGGGCGGTTCTTCGCAAAGATTAAAACCCCCCCCGGCGGGGTTGTc                                                                                      >  2:538078/1‑149 (MQ=18)
                                                   gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTTCCCGCCACCCCCCCGGGCGTTTCTTTGCAAACTTATAATCCCCTCCCCGCGGGGTTGTCCtt                                                                                   >  2:651382/1‑148 (MQ=255)
                                                     aTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCCCCCCCCACCCCCCCGGGCGCTGTTTTCTAAAAATCAAAATCGCTCCccgcgggattttgcttctc                                                                                >  2:612013/1‑137 (MQ=12)
                                                        ttAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGGCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCggg                                                                            <  1:424333/150‑1 (MQ=255)
                                                         tAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGgag                                                                          <  1:496543/151‑1 (MQ=255)
                                                                     cAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCAcc                                                                <  2:634788/149‑1 (MQ=255)
                                                                     cAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCAcc                                                                <  2:392186/149‑1 (MQ=255)
                                                                                 ccgtcaggATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCTGCCTTACCCCCCCCCCCCCTGCCCTTGCTTTTCAATCGTCAAATTCTCTTCCCGCGGTCTTTTTTTACCCCCGAGAGTGTTCTCCGCCCTacaacac                                                    >  1:199758/7‑148 (MQ=0)
                                                                                 ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTCCCCCCCCCCCCCCGGCCGGTGCTTCCCCACCTTCAAAACCCCCCCCCGCGGGTTTTTCCTACTCCGGAGGGGGTTTACCCacaaaaaaaaaaa                                                  >  2:552307/1‑146 (MQ=9)
                                                                                            gCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCCCCCCCCCCCCCCCCCGGCCGGTGCTTTGCAAAGGTCAAATCCCCCCCCCGCGGGGTTTTTCTTCTCCGGGGGGGGGTCACCCCCaaaaaaaaaaaaaaaaaaaaag                                       >  2:352581/1‑149 (MQ=9)
                                                                                             ccTTTCGCTTGATTTGATGTCGGGCAGTTTATGGCGGGCGCCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGc                                      <  1:403578/151‑1 (MQ=255)
                                                                                                       gatttgatGTCTGGCAGTTTATGGCGGGCGCCCTGCCCCCCACCCCCCGGGCCGCTGCTTTGCAACGTTCAAAACCCCCCCCCGCGGGTTTTTCCTTCTCCGGAGAGGGTTTACCCACAAAAAAAAGAAAAAAAAAAAAGCCCCGGCTTc                             >  2:87560/1‑150 (MQ=255)
                                                                                                        atttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCCCCCGGCCGGTGCTTCGCCAACTTTAAATCCCCTACCCGCGGGGTTGTTCTCCTCCGGCGGGTGTTCACCCACAAAACAAAGATAAAAAAAAAAGCCCCGttcttcg                           >  2:148155/1‑145 (MQ=2)
                                                                                                        atttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGGTGCTTCTCAAAGGTTCAAACCGCCCCCCGGCGGTTTGTCCTACCCCGGGGGGGGGTTCCCGAACAAACAACAAAAAAAACAAAAGACCCCGCCTTc                             >  1:317514/1‑150 (MQ=2)
                                                                                                        atttgatGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCCGTGCTTTGCAAAGGTTAAATTCCCTCCCCGCGGGTTTTTCCTTCTCCGGGGAGGGTTTACCCCCAAACAACAGAAAAAAAAAAAAAGCCAGGCTTcc                            >  2:523413/1‑150 (MQ=255)
                                                                                                          ttgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGAc                         <  1:792889/151‑1 (MQ=38)
                                                                                                            gatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGGTGCTTCGCCACCCTCCAATCCGCCCCCCGCCGGTTTTTCCTACTCCGGGGGGTGGTCACCCACCAACAACAAATAAAAAAAAAAGCCCACTCCTCCggccg                       >  1:237480/1‑147 (MQ=2)
                                                                                                                        tttATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGt             <  2:393855/149‑1 (MQ=38)
                                                                                                                              gcgggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTAttt      <  1:500565/150‑1 (MQ=38)
                                                                                                                                  gcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATg  <  2:566356/150‑1 (MQ=38)
                                                                                                                                              |                                                                                                                                            
TCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATG  >  NC_000913/3423146‑3423425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: