Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,553	A→T	K286* (AAA→TAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,553	0	A	T	100.0%	184.5 / NA	48	K286* (AAA→TAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (29/19);  total (29/19)

CTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTA  >  NC_000913/1756407‑1756700                                                                                                                                                   |                                                                                                                                                    ctGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCtaaaa                                                                                                                                                 <  1:578349/151‑1 (MQ=255)  tGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAc                                                                                                                                                <  2:761781/151‑1 (MQ=255)  tGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAc                                                                                                                                                <  2:378963/151‑1 (MQ=255)    aCGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAAcc                                                                                                                                               <  2:397996/150‑1 (MQ=255)     cGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCa                                                                                                                                             >  1:704384/1‑151 (MQ=255)       gCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCATATGCTGGATTCCATGATCTAAAACCCACg                                                                                                                                           >  1:362524/1‑151 (MQ=255)       gCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACg                                                                                                                                           >  2:75106/1‑151 (MQ=255)             tGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGa                                                                                                                                      >  2:386537/1‑150 (MQ=255)                                gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGGAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                                                                                   >  2:628000/1‑150 (MQ=255)                                gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCTGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                                                                                   >  2:367940/1‑150 (MQ=255)                                gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                                                                                   >  2:326096/1‑150 (MQ=255)                                gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                                                                                   >  2:215675/1‑150 (MQ=255)                                gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                                                                                   >  2:108646/1‑150 (MQ=255)                                 ggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                                                                                                  <  2:482287/150‑1 (MQ=255)                                 ggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                                                                                                 >  2:417618/1‑151 (MQ=255)                                  gtgtAGAAATCACGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGCGACGt                                                                                                                >  1:579063/1‑151 (MQ=255)                                    gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                                                                                              >  1:307585/1‑151 (MQ=255)                                                gTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATc                                                                                                   >  1:222378/1‑150 (MQ=255)                                                   gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                                                                                               >  2:559996/1‑151 (MQ=255)                                                       aagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg                                                                                           <  2:434940/151‑1 (MQ=255)                                                             tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa                                                                                     >  1:516729/1‑151 (MQ=255)                                                               ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                                                                                   <  2:633916/151‑1 (MQ=255)                                                               ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACGGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg                                                                                   <  1:795697/151‑1 (MQ=255)                                                                    ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg                                                                              <  2:516974/151‑1 (MQ=255)                                                                    ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg                                                                              <  1:796719/151‑1 (MQ=255)                                                                       gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg                                                                           >  1:423114/1‑151 (MQ=255)                                                                          gatgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg                                                                           >  2:109146/1‑148 (MQ=255)                                                                            tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc                                                                      <  1:139896/151‑1 (MQ=255)                                                                                 atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCCCTGCGCCCCAGCTGTTTGATTCCATGATCTAAATCCCACGCCCGAATCGCGCAGAGGCCGGTAACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg                                                                 <  1:674470/151‑1 (MQ=255)                                                                                 atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg                                                                 <  1:434037/151‑1 (MQ=255)                                                                                  tCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa                                                                <  1:215618/151‑1 (MQ=255)                                                                                   cGAAAAATGTTTCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACCCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGa                                                                <  2:3487/150‑1 (MQ=255)                                                                                       aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCg                                                           >  1:214073/1‑151 (MQ=255)                                                                                            tATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCaaa                                                       <  2:719736/150‑1 (MQ=255)                                                                                                      cGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAc                                             >  2:136262/1‑150 (MQ=255)                                                                                                      cGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAc                                             >  2:694389/1‑150 (MQ=255)                                                                                                      cGTAAAGTCGTTATCACTGCGACCCAGATGCCGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAc                                             >  1:720685/1‑150 (MQ=255)                                                                                                            gTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTgg                                      >  1:130646/1‑151 (MQ=255)                                                                                                            gTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTgg                                      >  1:282237/1‑151 (MQ=255)                                                                                                            gTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTgg                                      >  2:317052/1‑151 (MQ=255)                                                                                                            gTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTgg                                      >  2:139990/1‑151 (MQ=255)                                                                                                                         cGACCCAGATGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCC‑TCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATc                        <  2:624408/151‑1 (MQ=255)                                                                                                                           caccAGATACTGGATTCAATGATCTAAAACCCCCGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGCTAAATACCCGCTGGAAGCGGTTTCTATc                        <  1:21786/148‑1 (MQ=255)                                                                                                                                  tGCTGGATTCCATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGAc                <  2:13917/151‑1 (MQ=255)                                                                                                                                           ccATGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGa       >  1:106799/1‑151 (MQ=255)                                                                                                                                             aTGATCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAAc     <  2:423333/151‑1 (MQ=255)                                                                                                                                                aTCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTa  >  2:493480/1‑151 (MQ=255)                                                                                                                                                aTCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTa  >  1:502879/1‑151 (MQ=255)                                                                                                                                                aTCTAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTa  >  2:798182/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTA  >  NC_000913/1756407‑1756700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑