Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,133,3020TG54.4% 129.8 / 191.6 125H219P (CAC→CCC) yghSputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (10/47);  new base G (68/0);  total (78/47)
Fisher's exact test for biased strand distribution p-value = 6.72e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-18

Only 100 of 126 total aligned reads displayed.

TAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGG  >  NC_000913/3133157‑3133450
                                                                                                                                                 |                                                                                                                                                    
   ttGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGccc                                                                                                                                               <  1:1210846/150‑1 (MQ=255)
       gCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc                                                                                                                                           <  2:951465/150‑1 (MQ=255)
       gCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc                                                                                                                                           <  2:1025263/150‑1 (MQ=255)
       gCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc                                                                                                                                           <  2:858126/150‑1 (MQ=255)
             gcgcGGTTGTACTATTAACAATTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGGGAATTGCGCGTAGGGACGCTTGCAGAATTTCATCGGCGGGGGGTCTCCCCACCAgattga                                                                                                                                    >  2:453146/1‑146 (MQ=255)
              cgcgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCaa                                                                                                                                    <  1:1199013/150‑1 (MQ=255)
                cgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGa                                                                                                                                 <  2:1062979/151‑1 (MQ=255)
                cgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGCCCCTCCCGTTCCAGGa                                                                                                                                 >  2:351956/1‑151 (MQ=255)
                cgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAggg                                                                                                                                 >  1:934324/1‑150 (MQ=255)
                cgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCCTTCTAggg                                                                                                                                 >  1:487198/1‑150 (MQ=255)
                cgGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCAAGGa                                                                                                                                 >  2:598216/1‑151 (MQ=255)
                  gTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCTTTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTCAAggct                                                                                                                                >  2:1402664/1‑148 (MQ=255)
                  gTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTTAAGGAtt                                                                                                                               >  1:799064/1‑150 (MQ=255)
                  gTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGGTCaaaggt                                                                                                                                >  2:1131009/1‑146 (MQ=255)
                  gTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCCCCCCTCCAGATCAAAGag                                                                                                                                >  2:1473343/1‑149 (MQ=255)
                     gTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGCCCACCCACGTCAACGCTCttt                                                                                                                            >  1:430110/1‑151 (MQ=255)
                       aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCATAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgc                                                                                                                          <  2:1352920/151‑1 (MQ=255)
                       aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGGCCCTCCCGTTCAAGGATCttttt                                                                                                                          >  1:804047/1‑149 (MQ=255)
                       aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAGTTTAAGAGTCtttt                                                                                                                           >  2:1602886/1‑149 (MQ=255)
                       aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCCGTTTCAGGATCttttt                                                                                                                          >  1:1559483/1‑149 (MQ=255)
                       aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGGCGCCCATCCCGTTCAAGGGTCttttg                                                                                                                          >  1:969587/1‑149 (MQ=255)
                       aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTTAAGGGTCtttt                                                                                                                           >  2:312799/1‑149 (MQ=255)
                       aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCACCCCGTTCAAGGATCTTTg                                                                                                                           >  2:1006698/1‑150 (MQ=255)
                       aCTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCACCCCGGTTAAGGATCttttg                                                                                                                          >  2:873341/1‑149 (MQ=255)
                        cTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCGGTTAAGGATCTTTgcg                                                                                                                         >  1:1248250/1‑151 (MQ=255)
                        cTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTCAAGGATCTTTtcg                                                                                                                         >  2:776349/1‑151 (MQ=255)
                        cTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCACCCAGTTCCAGGATCTTTtcg                                                                                                                         >  1:535113/1‑151 (MQ=255)
                         tATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcgc                                                                                                                        <  2:601020/151‑1 (MQ=255)
                          aTTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGcc                                                                                                                       <  2:1527417/151‑1 (MQ=255)
                          aTTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGCCCATCCAGTTTCAGGGTCTTTtcgc                                                                                                                        >  2:1192232/1‑150 (MQ=255)
                          aTTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGTTCAAGGAGCttttctt                                                                                                                        >  1:1437108/1‑146 (MQ=255)
                            tAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCCCCAGTTCAAGGGTCTTTGGGCCAt                                                                                                                     >  2:486757/1‑151 (MQ=255)
                                 gtttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCCGCTCCAGGAGCTTTGCTGCATTTAAt                                                                                                                >  1:131293/1‑151 (MQ=255)
                                   ttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGt                                                                                                              <  1:1001801/151‑1 (MQ=255)
                                   ttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGt                                                                                                              <  2:893157/151‑1 (MQ=255)
                                   ttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGt                                                                                                              <  2:243998/151‑1 (MQ=255)
                                   ttgagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGt                                                                                                              <  2:1727660/151‑1 (MQ=255)
                                     gagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCCTCCAGTTTAAGGATCTTTGCGCCATTGAATGTCa                                                                                                            >  1:1732749/1‑151 (MQ=255)
                                     gagtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGTTCAAGGAGCTTTTCGCCCTTTAATGTCa                                                                                                            >  1:686984/1‑151 (MQ=255)
                                      agtttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTTCGCCATTGAATGGCAg                                                                                                           >  1:1147134/1‑151 (MQ=255)
                                        tttgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGtt                                                                                                         <  2:616142/151‑1 (MQ=255)
                                          tgTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTgc                                                                                                       <  1:1662040/151‑1 (MQ=255)
                                           gTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCCGTTCCAGGATCTTTTTGCCATTTGATGTCAGGTgtg                                                                                                      >  1:508593/1‑149 (MQ=255)
                                           gTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGTGCCCACCCCGGTTAAAGATCTTTTCGCCCTTGTATggtcgttgtg                                                                                                      >  1:1304050/1‑142 (MQ=255)
                                              gTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGGTCAAGGGTCTTTTCGCCCTTTAATGTCAGTTgcgggg                                                                                                   >  1:246929/1‑149 (MQ=255)
                                              gTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGGTCCAGGAACTTTTCGCCCTTTGATGTTAGTTgcggcg                                                                                                   >  2:423694/1‑151 (MQ=255)
                                               tGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTgcggcg                                                                                                   <  2:383373/150‑1 (MQ=255)
                                                  aTCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAAc                                                                                               <  2:131682/151‑1 (MQ=255)
                                                  aTCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGCCCATCCCGTTCCAGGTTCTTTTCGCCCCTGAATGTTCGTTGCGGCGGaa                                                                                                >  1:653900/1‑150 (MQ=255)
                                                  aTCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGGTCTTTTCGCCATTGAATGTCAGTTGGGGCGGaaa                                                                                               >  2:1101349/1‑150 (MQ=255)
                                                   tCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACg                                                                                              <  1:1525938/151‑1 (MQ=255)
                                                         cGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGGTCAAGGGTCTTTGCGCCCTTGGATGTCAGGTGCGGCGGAAAGGCGGtt                                                                                        >  2:1133332/1‑151 (MQ=255)
                                                               atcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGa                                                                                                                                 <  1:292210/104‑1 (MQ=255)
                                                               atcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTcc                                                                                  <  1:1573851/151‑1 (MQ=255)
                                                               atcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTcc                                                                                  <  2:833835/151‑1 (MQ=255)
                                                               atcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGCCCCTCCAGTTTCAGGAACTTTTTTCCATTGAATGTCCGTTTGGGGGGAACGGGGGttttttt                                                                                   >  1:1507334/1‑149 (MQ=255)
                                                               atcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGGTCAAGGAACTTTTCGCCCTTTAATGGCAGGTGTGGCGTGAAGGCGTttttttt                                                                                   >  2:761096/1‑149 (MQ=255)
                                                               atcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGCTCAAAGAACCTTGGGCCAATGAAAGGCAGTTTGGCCGCAACGGCGAattttttt                                                                                  >  1:397205/1‑149 (MQ=255)
                                                               atcatcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCCCCCGTTCAAGGGTCTTTTCGCCCTTGAATGTCAGTTGGGGCGGAACGGGGGttttttt                                                                                   >  2:306911/1‑149 (MQ=255)
                                                                   tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGCCCCTCCCCGTCAAAGATTCTTTCGCCATTTTATGTTCTTTGGCGCGGAAAGGCGATTTTTTTCtctt                                                                              >  1:378767/1‑148 (MQ=255)
                                                                    ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTaa                                                                             <  2:364547/151‑1 (MQ=255)
                                                                    ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGTTTCAGGATCTTTGCGCCATTTTATGTCAGGTGCGGCGTCACGGCGCGTTTTTTCtttta                                                                             >  1:803512/1‑147 (MQ=255)
                                                                    ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCCTTTAATGTCCGTTGCGGCGTAACGGCGATTTTTTTCTTTaa                                                                             >  2:31815/1‑151 (MQ=255)
                                                                    ccccTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGGTCAAGGGTCTTTTCGCCATTGGATGTCAGTTGCGGGGTAACGGCGGTTTTTTTCTGtta                                                                             >  2:1377830/1‑149 (MQ=255)
                                                                    ccccTATCCATTTTGGAGATCAGGAAAGCGCGGCGTGAATTGCGCGAAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGTCCCCCCACCTCCAGCAACTTTTGGCCCTTGGCTGTCACCTGCGGGGGAAACGCGGTTTTTTCCTGTaa                                                                             >  2:588541/1‑151 (MQ=255)
                                                                       cTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGGTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGc                                                                          <  1:326107/151‑1 (MQ=255)
                                                                           ccATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCCTTGTATGTCAGTTGCGGCGTGACGGCGGTTTTTTTCTGTTAAGCTGc                                                                       >  1:610018/1‑150 (MQ=255)
                                                                           ccATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCCCCCGTTCAAGGATCTTTTCGCCCTTTAATGTCAGTTGCGGCGTAACGGCGGTTTTTTCCttttaagccgc                                                                       >  1:1104486/1‑140 (MQ=255)
                                                                           ccATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCAGGTCAAGGGTCTTTTTGCCATTTGATGTCAGTTGGGGGGGAACGGGGGTTTTTTTCTGTAAcgccgc                                                                       >  2:785270/1‑150 (MQ=255)
                                                                             aTTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAg                                                                    <  2:1512408/151‑1 (MQ=255)
                                                                              ttttGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGGCCCTCCAGTTCAAAGGTCTTTTTGCCCTTGAATGTCAGTTGTGGGGGAAAGGCGGGTTTTTTTTGTTACGCTGCCCGt                                                                   >  2:741907/1‑151 (MQ=255)
                                                                                 tGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtgg                                                                <  1:42756/151‑1 (MQ=255)
                                                                                  ggTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCAGTTTAAGGATCTTTTGGCCATTTGATGTCAGGTGCGGGGGAAAGGGGGTTTTTTTCTTTAACGGCTCCAGtttgg                                                               >  2:917265/1‑148 (MQ=255)
                                                                                     gATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTTAAAGATCTTTGCGCCATTTAATGTCAGTTTCGGCGTAACGGCGGTTTTTTTCTTTAACGCTGcccggttggggg                                                            >  1:111193/1‑141 (MQ=255)
                                                                                       tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCGGTCAAGGGTCTTTTCGCCCTTGAATGTCAGTTGGGGCGGAACGGCGGTTTTTTTCTGTAAAGCTGCCCGTtgggggggg                                                          >  2:40082/1‑148 (MQ=255)
                                                                                       tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGGTCAAGGATCTTTTCGCCCTTGAAAGTCAGTTGCGGGGGAAAGGCGATTTTTTTCTGTAAAGCTGCCCGTtggtgggcg                                                          >  2:1700760/1‑148 (MQ=255)
                                                                                       tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGGTCAAGGATCTTTTCGCCATTGAATGTCAGTTGCGGGGTAACGGCGGTTTTTTTCTTTAAAGCTGCCCGGtggggggc                                                           >  2:483113/1‑148 (MQ=255)
                                                                                       tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCAAGGATCTTTTTGCCCTTGGATGTCAGTTGCGGCGTAACGGCGGTTTTTTTCTGTAACGCCGCCCGTTGGGGGTc                                                           >  2:1138027/1‑150 (MQ=255)
                                                                                        cAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCATCCCGTTCAAGGATCTTTGCGCCATTTAATGGCAGGTGCGGGGTAACGGCGATTTTTTTCTTTAAAGCGGCCCGGtggggggggg                                                         >  2:454628/1‑147 (MQ=255)
                                                                                           gAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAgg                                                        <  2:437027/149‑1 (MQ=255)
                                                                                            aaaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGtt                                                      <  1:452996/150‑1 (MQ=255)
                                                                                                cgcgGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTAcgc                                                 <  1:544047/151‑1 (MQ=255)
                                                                                                  cggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTAcgcg                                                <  2:1440618/150‑1 (MQ=255)
                                                                                                  cggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTAcgcg                                                <  1:543036/150‑1 (MQ=255)
                                                                                                          aTTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCgg                                       <  2:1701527/151‑1 (MQ=255)
                                                                                                          aTTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCATTTAATGTCAGTTGCGGCGGAACGGCGGTTTTTTTCTGTAACGCTGCCAGTTGGGGGGCAGGGTTACGCGCAAACGCgg                                       >  2:1368588/1‑151 (MQ=255)
                                                                                                            tGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGtt                                     <  1:1424573/151‑1 (MQ=255)
                                                                                                             gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCACTTTAAGGGTCTTTTTGCCCTTTTATGTCAGTTTCGGCGGAACGGCGGTTTTTTTCTTTTACGCTGGCAGTTGGTGGGTGGGTTTATGGGCAAACGCGGgtt                                    >  1:1500567/1‑151 (MQ=255)
                                                                                                               gcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCCGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGGGGCGTAAAGGCGATTTTTTTCTGTAAAGCTGCCAGTTGGGGGGCAGGGTTTCGCGCAAAAGCGGgtttt                                  >  2:155899/1‑149 (MQ=255)
                                                                                                                  tAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTtca                               <  2:1248882/151‑1 (MQ=255)
                                                                                                                       aCGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCaa                           <  1:1284409/150‑1 (MQ=255)
                                                                                                                          cTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGc                        <  2:1416674/150‑1 (MQ=255)
                                                                                                                           ttGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCCTTGAATGTCAGTTGCGGCGGAACGGCGATTTTTTTCTTTAAAGCTGCCAGTTGGGGGTCCGGGTTACGCGCAAACGCGGGTTTTTCATCAATGCCa                      >  2:1319362/1‑151 (MQ=255)
                                                                                                                                 aaTTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGAc                  <  2:955428/149‑1 (MQ=255)
                                                                                                                                        tCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATcaaca         >  1:1747693/1‑151 (MQ=255)
                                                                                                                                            cggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATAc     <  2:424280/151‑1 (MQ=255)
                                                                                                                                            cggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATAc     <  1:760720/151‑1 (MQ=255)
                                                                                                                                            cggGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATAc     <  1:1402036/151‑1 (MQ=255)
                                                                                                                                               ggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACggg  >  1:1640804/1‑151 (MQ=255)
                                                                                                                                                gtgtCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACggg  <  1:224236/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TAGTTGGGCTGCGGCGCGGTTGTACTATTAACAGTTTGAGTTTGTAGTGCATCCATTCGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGGTGGTCAGGTTTACGCGCAAACGCGGTTTGTTCATCAATGCCAAGACGAATCAACAATACGGG  >  NC_000913/3133157‑3133450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑