Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,6980TC52.4% 72.6 / 199.7 145D38G (GAT→GGT) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base T (47/22);  new base C (0/76);  total (47/98)
Fisher's exact test for biased strand distribution p-value = 1.78e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-16

Only 100 of 151 total aligned reads displayed.

CTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTG  >  NC_000913/3665548‑3665842
                                                                                                                                                       |                                                                                                                                                  
ctacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                                    >  1:783593/1‑151 (MQ=255)
ctacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                                    >  1:370786/1‑151 (MQ=255)
ctacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                                    >  2:1666513/1‑151 (MQ=255)
ctacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                                    >  2:240780/1‑151 (MQ=255)
ctacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                                    >  2:502895/1‑151 (MQ=255)
  acAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCCTc                                                                                                                                                   >  2:1608757/1‑150 (MQ=255)
     gTGATGAACGAGTCATGAAGAAACTTTTTTATGTGAATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                               >  1:1296655/1‑151 (MQ=255)
     gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                               >  1:1428454/1‑151 (MQ=255)
           aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGGATTTGGCTTGCATCCGCAAAAAcc                                                                                                                                         <  2:1516039/151‑1 (MQ=255)
           aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAcc                                                                                                                                         <  1:165243/151‑1 (MQ=255)
           aaCGAATAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAc                                                                                                                                          <  2:1739418/150‑1 (MQ=255)
                   aTGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CAc                                                                                                                                 >  2:1073568/1‑150 (MQ=255)
                            cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTaaa                                                                                                                       >  1:403696/1‑151 (MQ=255)
                            cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTaaa                                                                                                                       >  2:223075/1‑151 (MQ=255)
                             ttttttATGTGCATGAAGATTTAATCCCTCCTCCTTGAGAATCGGGAAAAATAATGAAAGCGTTCCCTTTGACACAAATACAAAATTTCCAACA‑CCCTTATCTCCCCAATTTTGGCTTGCATCCCAAAAAACCAGGT‑CACCGCCATTaaaa                                                                                                                      <  1:550911/151‑1 (MQ=255)
                             ttttttATGTGCATAAAGATTTAATGCCTCCTCCTTGACCATCGGGAAAAATAATAAAAGCGTTTCCCTTGACACGAATACAAAATTTTCAACA‑CCCTTATCTACTCGATTTTGGCTTGCATCCGAAAAAACCAGGT‑CACCGCCATTaaaa                                                                                                                      <  2:541546/151‑1 (MQ=255)
                                           aaaGTTTTAAGGCCTCCTCTTTGAGCATGGGAAAAAAAAATAAAGGCGGGCCCTTTGACACAAATAAAAAATTTCCACCA‑CCTTTTTCTACAAAATTTTGGTTTGCACCCGAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCAcc                                                                                                        <  1:941795/150‑1 (MQ=255)
                                              gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                     >  2:182114/1‑151 (MQ=255)
                                               ttttAATGCCTCCCCCTTGAGCATCGGAAAAAATAATGAAAGCGTCTCCCTTGACACAAATAAAAAATTTACAACC‑CACTTATCTACTAGAATTTGGCTTCCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCAt                                                                                                      <  1:930621/148‑1 (MQ=255)
                                               ttttAACCCCCCCTCCTTGACCATCGGAAAAAATAATAAAAGCGTCTCCCTTGACCCAAAAACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCACCCGCAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCAt                                                                                                      <  2:261334/148‑1 (MQ=255)
                                                tttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTaa                                                                                                   >  1:656557/1‑151 (MQ=255)
                                                 ttAATGCCTCCTCCTTGACCTGCGGGAAAAATAATAAAAGCGTGTCCCTTGACACAAAAAAAAAATTTTCAACC‑CACTTATCTCCTCGAATTTGGCTTGCTCCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                  <  2:1020234/151‑1 (MQ=255)
                                                  tattcccccctcttTGAACATCGGGAAAAAAAATAAAGGCGTGTCCTTTGACACAAATAAAAAATTTTCAACC‑CACTTATCTACTCAATTTTGGCTTGCACCCGAAAAAACCAGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  1:741422/143‑1 (MQ=255)
                                                  tAATGCCTCCTCCTGGAGCATGGGAAAAAATAATAAAAGCGTTTCCCTTGACACAAAAAAAAAATTTTAAACA‑CACTTATCTACCCAATTTGGGTTTCCACCCCAAAAAACCGGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  1:134141/151‑1 (MQ=255)
                                                  tAATGCCCCCTCCTTGAGCATCGGGAAAAATAATAAAGGCGTGTCCCTTGACACAAATAAAAAATTTTCAACA‑CACTTTTCTACTCGAATTTGGCTTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:132552/151‑1 (MQ=255)
                                                  tAATCCCCCCCCCTGGAGCACGGGGAAAAAAAATGAAAGCGTTTCCCTTAACACAAATAAAAAATTTACAACA‑CACTTATCTTCTCGATTTTGGCTTCCCCCCGAAAAACCCAGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:1369274/151‑1 (MQ=255)
                                                  aagacacacctcctTGAGCAGGGGGAAAAAAAAGGAAGCGGTGCCCCTTACAAAAAATAAAAAATTTCCAACA‑CATTTATCTCCTGGATTTGGGCTTCCGCCCGAAAAAACCAGCT‑CCCCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:1433344/143‑1 (MQ=255)
                                                         cccccctTGAGCATGGGGAAAAAAAAAAAAAGGGTTTCCCTTAACACAAAAAAAAAATTTTCAACC‑CCCTTATCTACTCAAATTTGGCTTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                          <  2:1564374/147‑1 (MQ=255)
                                                           ctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 >  1:176588/1‑142 (MQ=255)
                                                           ctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgaga                                                                                        >  2:942320/1‑151 (MQ=255)
                                                           ctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgaga                                                                                        >  2:830458/1‑151 (MQ=255)
                                                            tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                       >  1:883153/1‑151 (MQ=255)
                                                            tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                       >  1:233037/1‑151 (MQ=255)
                                                             cctGGAGCATGGGAAAAAATAATGAAAGCTTGCCCCTTGACAAGAATAAAAATTTTACAACC‑CCCTTTTCTCCTGAATTTTGGCTTGCACCCGAAAAACCGAGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                      <  1:1176180/151‑1 (MQ=255)
                                                                tGAGCTGCGGGAGAAAAAATAAATGCGTGTCCCTTGACACAATTACAAAATTTTCAACC‑CCCTTATTTACTCAAATTTGGTTGGCATCCGAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatata                                                                                   <  2:1469805/151‑1 (MQ=255)
                                                                tGAGCATGGGGAAAAAAAATAAAAGCGTGTCCTTTAACACAAAAAAAAAATTTTCAACA‑CACTTATCTACTCAAATTTGGCTTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatata                                                                                   <  2:593567/151‑1 (MQ=255)
                                                                   gaaTCGGGAAAAAAAAAAAAGGCGTGTCCTTTGACACAAATAAAAAATTTCCAACA‑CACTTATCTACTCAATTTGGGTTTCCACCCAAAAAAACCGGGA‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑tt                                                                                <  1:972979/148‑1 (MQ=255)
                                                                   gCATGGGGAAAAAAAATGAAAGCGTGTCCTTTGACACAAATACAAAATTTACAACA‑CCTTTATCTACGCAAATTTGGTTTCCACCCAAAAAAACCAGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑tt                                                                                <  1:1188096/150‑1 (MQ=255)
                                                                   gCATGGGGAAAAAAAAGGAAAGCGTGTCCTTTGACACAAATAAAAAATTTCAAACA‑CATTTATCTACTCAATTTTGGTTTCCACCCAAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑tt                                                                                <  1:404371/150‑1 (MQ=255)
                                                                    cATGGGGAAAAAAAAAAAAGGCGGGCCCCTTGACACAAAAAAAAAATTTTCAACA‑CACTTATCTCCTCGAATTTGGCTTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑tt                                                                                <  2:440065/149‑1 (MQ=255)
                                                                      tggggAAAAAAAATAAAGGCGTTCCCCTTGACAAAAAAAAAAATTTTTCAACC‑CCTTTATCTACTCAATTTTGGTTTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATg                                                                            <  2:652499/149‑1 (MQ=255)
                                                                       cggaaaaaaTAATGAAGGCGTGCCCCTTGACACAAATAAAAAATTTCCAACA‑CACTTATCTACTCAATTTGGGTTTCCACCCGAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGt                                                                           <  1:240709/145‑1 (MQ=255)
                                                                         ggAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGt                                                                           >  1:104140/1‑149 (MQ=255)
                                                                         ggAAAAATAATGAAAGCGTGCCCCTTGCCAAAAAAACAAAATTTACAACA‑CCTTTATCTACTCGATTTTGGCTTGCACCCGAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTc                                                                          <  2:427724/150‑1 (MQ=255)
                                                                          gaaaaaTAATAAAAGCGTTTCCTTTGACACAAATAAAAAATTTACAACA‑CACTTATCTACTCAATTTTGGTTTGCACCCGAAAAAACCGGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCt                                                                         <  2:559190/147‑1 (MQ=255)
                                                                          aaaaaaaaaTAAAAGCGTGCCCCTGGACAAAAAAAAAAAATTTACAACA‑CTCTTATCTACTCAATTTGGGCTTGCACCCGAAAAAACCAGGT‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCt                                                                         <  2:1647778/147‑1 (MQ=255)
                                                                          aaaaaaTAATGAAGGCGTTCCCTTTGACACAAAAAAAAAATTTCCAACC‑CCTTTATCACCTAAATTTTGGCTTCCACCCAAAAAAACAGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCt                                                                         <  1:373162/147‑1 (MQ=255)
                                                                            gaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTg                                                                       >  1:1362513/1‑150 (MQ=255)
                                                                            gaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTg                                                                       >  1:75242/1‑150 (MQ=255)
                                                                              aataatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCAt                                                                    >  1:1001312/1‑151 (MQ=255)
                                                                                  atGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACg                                                                 >  2:1415611/1‑150 (MQ=255)
                                                                                     aaaGCGTGTCCTTTGACACAAATACAAAATTTACAACA‑CATTTATCTACTCAAATTTGGCTTGCACCCGAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAAt                                                             <  1:1738554/151‑1 (MQ=255)
                                                                                       aGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTa                                                           >  1:660473/1‑151 (MQ=255)
                                                                                       aGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTa                                                           >  2:473775/1‑151 (MQ=255)
                                                                                           tgtCCCTTGCCACAAATAAAAATTTTTCACCA‑CTTTTATCTACTGAATTTTGGTTTGCACCCGAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGAc                                                        <  1:1431454/150‑1 (MQ=255)
                                                                                           tgtCCCTTAACACAAAAAAAAAATTTTCAACC‑CCTTTATCCACTCGATTTTGGTTTGCACCCCAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGAc                                                        <  2:1041056/150‑1 (MQ=255)
                                                                                           tcccctttaacacAAATAAAAAATTTACAACA‑CACTTATCTACTCAAATTGGGTTTGC‑CCCCAAAAAAACAGGTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGAc                                                        <  1:810116/141‑1 (MQ=255)
                                                                                              cccTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAAtt                                                    >  1:564778/1‑151 (MQ=255)
                                                                                                cttgaaaaaaaaaaaaaaTTTCAAACA‑CACTTATCTACTCAATTTGGGTTTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTcc                                                  <  2:481328/137‑1 (MQ=255)
                                                                                                    ccacaAAAACAAAATTTTAAACA‑CCCTTTTCTACTGAATTTTGGCTTCCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                              <  2:112370/150‑1 (MQ=255)
                                                                                                    acaaaaaTAAAAAATTTACAACA‑CACTTATCTACTAAATTTTGTCTTGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                              <  1:1319732/146‑1 (MQ=255)
                                                                                                       caaaaaaaaaaTTTACAACC‑CATTCATCTACTCGATTTGGGCTTGCACCCGAAAAAACCAGGT‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  1:501789/144‑1 (MQ=255)
                                                                                                       cGAATACAAAATTTTCAACA‑CACTTATCTCCTCAATTTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  1:1456933/151‑1 (MQ=255)
                                                                                                       aaaaTAAAAAATTTACAACA‑CATTTATCACCTCAATTTGGGCTTCCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  1:1526174/149‑1 (MQ=255)
                                                                                                        gAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                          >  1:273936/1‑151 (MQ=255)
                                                                                                         aaTAAAAAATTTTCAACA‑CACTTATCTACTAGATTTTGGTTTGCATCCAAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                         <  1:446187/151‑1 (MQ=255)
                                                                                                              aaaaTTTACACCC‑CACTTACCTACAAAATTTTGGCTTGCACCCGAAAAAACAAGGT‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGc                                    <  1:563752/151‑1 (MQ=255)
                                                                                                                aaTTTTCAACA‑CCCTTATCTACTCAAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                  <  2:964626/151‑1 (MQ=255)
                                                                                                                aaTTTACAACC‑CATTTATCTACTAAATTTTGTTTTGCACCCAAAAAACCCGGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                  <  1:1756761/151‑1 (MQ=255)
                                                                                                                 aatttCAACCCCACTTATCTACGAGATTTTGGCCTACACCCAAAAAAACCAGTA‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                  <  1:586935/149‑1 (MQ=255)
                                                                                                                   tttCAACC‑CCCTTATCTACTCAAATTTGGCTTCCACCCCAAAAACCCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                               <  2:660764/151‑1 (MQ=255)
                                                                                                                   tttCAACC‑CACTTATCTCCTCAAATTTGGCTTGCACCCGAAAAACCCAGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                               <  2:763095/151‑1 (MQ=255)
                                                                                                                   tttCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                               >  1:1201851/1‑151 (MQ=255)
                                                                                                                   ttacAACA‑CTCTTATCTACTCGATTTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                               <  1:429821/148‑1 (MQ=255)
                                                                                                                     tCAACA‑CTTTTTTTTATTCAACTTTGGCTGGCACCAGAAAAAACCAGTC‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                             <  1:1717404/151‑1 (MQ=255)
                                                                                                                         acccatttttCTACTCGATTTTGGCTTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                          <  2:1061141/142‑1 (MQ=255)
                                                                                                                         acccacTTATCTACTCAAATTTGGTTTGCACCCAAAAAACCCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                          <  1:1115305/148‑1 (MQ=255)
                                                                                                                          a‑cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGTTTGCATAGTTGACTTa                        >  2:152743/1‑151 (MQ=255)
                                                                                                                            cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCCTTAAAATAACGATATTCACCATTAACCATGGGGAGAAAATA‑TTTTTTTTTTTCATAAGCAATTAAACAATTTCCATTTAGTGGTTGCATAGTTGACttta                       >  2:403026/1‑149 (MQ=255)
                                                                                                                            cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTCGTGATTGCATAGTTGACTTaa                       >  2:1203769/1‑151 (MQ=255)
                                                                                                                            cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                       >  1:1277684/1‑151 (MQ=255)
                                                                                                                              cTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                     >  1:1484799/1‑151 (MQ=255)
                                                                                                                              cTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                     >  2:1431892/1‑151 (MQ=255)
                                                                                                                               ttATCTTCTAAATTTGGGCTTGCATCCAAAAAACCCAGGA‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:1547916/151‑1 (MQ=255)
                                                                                                                               ttATCTCCTAGATTTTGGTTTGCATCCAAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:783992/151‑1 (MQ=255)
                                                                                                                               ttATCTACTGAAATTTGGCTTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:380539/151‑1 (MQ=255)
                                                                                                                               ttATCTACTCAATTTGGGCTTCCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  1:613049/151‑1 (MQ=255)
                                                                                                                               ttATCTACTCAAATTTGGCTTGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:516940/151‑1 (MQ=255)
                                                                                                                               ttATCTACTAAATTTTGTTTTGCACCCACAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  1:962260/151‑1 (MQ=255)
                                                                                                                               ttATCTACTAAAATTTGGTTTGCACCCGAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:766984/151‑1 (MQ=255)
                                                                                                                               ttATCTACAAAATTTTGGCTTGCCTCATAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  1:516263/151‑1 (MQ=255)
                                                                                                                                tacctactaAAATTTGGCTTGCACCCACAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  1:229326/148‑1 (MQ=255)
                                                                                                                                tATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                   >  2:984594/1‑151 (MQ=255)
                                                                                                                                          aaTTGGGCTTGCACCCGAAAAAACCAGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat         <  2:1727678/151‑1 (MQ=255)
                                                                                                                                               ggCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa     >  2:1373628/1‑150 (MQ=255)
                                                                                                                                                gtttGCATCCGAAAAAACCAGGA‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  1:1373011/148‑1 (MQ=255)
                                                                                                                                                gCTTGCACCCGAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  2:1288030/150‑1 (MQ=255)
                                                                                                                                                gCTTGCACCCAAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  2:1285469/150‑1 (MQ=255)
                                                                                                                                                gCTTGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  1:473609/150‑1 (MQ=255)
                                                                                                                                                gCTTCCACCCGAAAAACCCGGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  2:802249/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
CTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACA‑CACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTG  >  NC_000913/3665548‑3665842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑