Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG56.2% 69.4 / 133.8 91V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (7/32);  new base G (50/0);  total (59/32)
Fisher's exact test for biased strand distribution p-value = 9.94e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-13

GCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354144‑354436
                                                                                                                                                  |                                                                                                                                                     
gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAg                                                                                                                                                  <  1:96214/150‑1 (MQ=255)
gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAg                                                                                                                                                  <  2:1328057/150‑1 (MQ=255)
gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAg                                                                                                                                                  <  2:875583/150‑1 (MQ=255)
 cATCCGTGACGCTGACGGGTATCACTTTATTCTCGGGCGCGCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCCTCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGa                                                                                                                                                 <  1:346287/150‑1 (MQ=255)
    ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGGAAAATgtg                                                                                                                                             >  2:556647/1‑149 (MQ=255)
    ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑A‑GGGTGGGGGGAAAAATgcg                                                                                                                                             >  1:271142/1‑151 (MQ=255)
    ccGCGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGGTTGAAGAGAGCATCTCCAGCCATCCGGGCGTTGACGCAGGGGCGG‑G‑GAGTGGGGCGTAAggagcg                                                                                                                                             >  1:250021/1‑146 (MQ=255)
       tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTg                                                                                                                                         <  1:1173436/151‑1 (MQ=255)
             tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAggg                                                                                                                                   <  2:263355/151‑1 (MQ=255)
                cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAg                                                                                                                                <  2:1257544/151‑1 (MQ=255)
                cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGATGCG‑CTGAAAGGGCAg                                                                                                                                >  2:396374/1‑151 (MQ=255)
                         cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGATGGG‑CTGGAAGGGGGggtggcgggg                                                                                                                       >  1:302775/1‑149 (MQ=255)
                         cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGAAGGG‑CCGGAAGGGGAgggggggggg                                                                                                                       >  1:1706663/1‑149 (MQ=255)
                         cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAAATGCG‑CTGAAAGGGCCgggggggggg                                                                                                                       >  2:687473/1‑149 (MQ=255)
                         cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGGATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGATGCG‑CCGGAAGGGCAgggggggggg                                                                                                                       >  1:323511/1‑149 (MQ=255)
                                  cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                                                                                                              <  2:519662/151‑1 (MQ=255)
                                      cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTc                                                                                                          <  2:208891/151‑1 (MQ=255)
                                      cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTc                                                                                                          <  1:785749/151‑1 (MQ=255)
                                      cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGGAAGATGGG‑CTGAAAGGGGAGGGGGGGGGGGGGTTTGTCAtt                                                                                                           >  2:1228450/1‑150 (MQ=255)
                                          cTGAAGATGTAATTAACGTTGCCGGGGCTCCGGTGGGGACGCGGGAGATTGAAGAGAGTATCCCCAGCCATCCGGGCGGTGCCGGAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaa                                                                                                      <  2:1007897/151‑1 (MQ=255)
                                             aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                    <  1:76364/150‑1 (MQ=255)
                                             aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                    <  2:640530/150‑1 (MQ=255)
                                             aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                    <  1:1589318/150‑1 (MQ=255)
                                              cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagag                                                                                                  <  2:689963/151‑1 (MQ=255)
                                                aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGATGCG‑CTGGAAGGGGCGGGGGGGGGGGGGGTTGTCATTTTGAAaaagag                                                                                                >  1:1414580/1‑151 (MQ=255)
                                                aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGAAAAATGCG‑CTGGAAGGGGAGGGGGGGGGGGGGTTTTGTTTTCCGGAagaggg                                                                                                >  2:195548/1‑149 (MQ=255)
                                                  gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGGGGAAGATGGG‑CCGAAAGGGCAGGGGGGGGGGGGGTTTTTTCTTTCGAAAAAGGGCg                                                                                              >  2:877783/1‑151 (MQ=255)
                                                  gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGATGTG‑CTGAAAGGGGAGGGGGGGGGGGGGGTTGGCCTTTCGAAAGAGAGCg                                                                                              >  2:921451/1‑151 (MQ=255)
                                                  gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGATGCG‑CTTAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAagagaggg                                                                                              >  2:895413/1‑149 (MQ=255)
                                                  gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGAAAAACGCG‑CTGAAAAGGCGGGGGGGGGGGGGGGTTTTCATTTCGGAaaaggggg                                                                                              >  2:1452086/1‑147 (MQ=255)
                                                  gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGG‑G‑GGTTGGGGGGGAAAAAGGG‑CGGGAAAGGCAGGGGGGGGGGGCGGTTGTCAGTCCGGAAAAGGGCg                                                                                              >  2:446717/1‑151 (MQ=255)
                                                   tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGGGAAAGATGCG‑CCGAAAGGGGAGGGGGCGGGGGCGGTTGTCATTTCGAAAGAGAGCGa                                                                                             >  2:91286/1‑151 (MQ=255)
                                                   tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGAAGCG‑CTGGAAGGGGAGGGGGCGGGGGGGGTTGTTATTTCGGAAGAGGGCGa                                                                                             >  2:1098446/1‑151 (MQ=255)
                                                   tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGGAAAATGGG‑CTGAAAAGGCAGGGGGGGGGGGGGGTTTGCATTCCGGAagagagggg                                                                                             >  2:173005/1‑148 (MQ=255)
                                                   tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGGTGCCGAAGGGGCGG‑TGGGTGGGGGGGAAAGA‑GGG‑CTGGAAGGGGAGGGGGGGGGGGGGGGTGTTGTTCCGGAAGAGAGCgg                                                                                             >  1:751975/1‑150 (MQ=255)
                                                    aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGATGGG‑CTGGAAGGGGGGGGGGGGGGGGGGGTTGTTATTTCGAAAGAGAGCgga                                                                                            >  1:1279925/1‑149 (MQ=255)
                                                    aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGGAAGATGCG‑CCGGAAGGGGGGGGGGGGGGGGGGGTTGTCATTCCCAAaaagggggaa                                                                                            >  1:1608358/1‑145 (MQ=255)
                                                        aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTc                                                                                        <  1:920951/151‑1 (MQ=255)
                                                         aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGATGCG‑CTGAAAGGGGAGGGGGCGGTGGGGGTTGTCATTTCGAAAAAGAGCGACAGt                                                                                         >  1:863865/1‑149 (MQ=255)
                                                               ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  1:1447156/150‑1 (MQ=255)
                                                               ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  1:983026/150‑1 (MQ=255)
                                                               ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                                 <  2:1167039/151‑1 (MQ=255)
                                                                cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                                <  2:1019826/151‑1 (MQ=255)
                                                                   gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGAAAGATGGG‑CTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTTCGCAAGAGAGCGGCAGGCTGGGAGAtggg                                                                             >  1:769912/1‑148 (MQ=255)
                                                                     aTCGGCTGGGGACGCGGGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa                                                                           <  2:323609/151‑1 (MQ=255)
                                                                        ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCG‑GGTTGGGGGGAAA‑AAGCG‑CTGAAAGGGCAGGGGGCGGGGGCGGTTGTCTTTCCGAAAGAGAGGGACAGGCTGGGAGATCGGGAttg                                                                        >  1:1580470/1‑149 (MQ=255)
                                                                        ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGGGGTGGGGGTTGTCATTCCGAAAGAGAGCGGCAGTCCGGGAGATCGGGAtgg                                                                        >  2:256195/1‑150 (MQ=255)
                                                                        ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGAAAGATGCG‑CCGAAAGGGGCGGGGGGGGGGGGGGTTGTCATTTCGGAAGAGGGCGACAGGCTGGGAGAttggggtgg                                                                        >  2:852320/1‑143 (MQ=255)
                                                                        ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGGTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGATGGG‑CTGAAAGGGGAGGGGGGGGGGGGGTTTGTTATTTCGAAAGAGAGGGGCAGTCTGGgagaggggggtgg                                                                        >  2:679836/1‑142 (MQ=255)
                                                                         gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGCTGGGGGAAATAGGGG‑CTCAAAGGGCCGGGGGCGGGGGCGGTTTTTCTTTCGCAAGAGAGGGACCGGCTGGGAggacgggaggtg                                                                       >  1:352308/1‑140 (MQ=255)
                                                                          cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                      <  2:1559256/151‑1 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                                <  1:246085/128‑1 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGAAGTG‑CCGAAAGGGGAGGGGGCGGGGGGGGTTGTCATTTCGAAAGGGAGCGACAGTTTGGGAGATCGTGGGGTGGGGCACTCGCCaga                                                         >  2:1657501/1‑151 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGGGAAAGATGCG‑CTGAAAGGGGAGGTGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCCCTCGCAaga                                                         >  2:1170223/1‑151 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGGAGATCGGGGTGGGGGGCACTCGCAagg                                                         >  2:124186/1‑150 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGTGGAAGAAGCG‑GTGGAAGGGGAGGCGGCGGGGGGGGTTGTCATTCCGAAAGAGGGCGCCCGGCTGGAAGGTCGCGGTGTTGTGCACTCGCAcga                                                         >  1:590194/1‑151 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGAAGCGCCGGAAGGGGCGGGTGG‑GGGGGGGGTTGGCATTCCCGAAGAGAGCGGCCGTCTGGAAGATCGTGCTGTGGCGCACTCGCAaga                                                         >  1:1747695/1‑151 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAAAAGCG‑CTGGAAGGGGAGGGGGGGGGGGGGGTTGTTATTCCGAAAGAGAGCGACAGGCTGGAAGAACGTGATGTGGCGCCCCCGCAaga                                                         >  1:783861/1‑151 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGATGCG‑CTGGAAGGGGAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCCGGAAGATCGTGGTGTGGGGCAccccccaga                                                         >  1:732604/1‑143 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGAAGCG‑CTGAAAGGGGAGGGGGGGGGGGGGGTTTTTATTCCCAAAGAGAGGGACAGTCTGGAAGAt                                                                                >  2:246156/1‑128 (MQ=255)
                                                                                       agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGTTGGGGGGAAAGATGCG‑CTGAAAGGGGCGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAaga                                                         >  2:131165/1‑151 (MQ=255)
                                                                                         aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                        <  2:575913/150‑1 (MQ=255)
                                                                                         aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                        <  2:941034/150‑1 (MQ=255)
                                                                                         aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                       <  2:1029382/151‑1 (MQ=255)
                                                                                         aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAAAAGCG‑CCGGAAAGGGAGGGGGGGGGGGGGTTTTGTATTCCGAAAGAGAGCGACAGGCTGGAAGAACGTGGTGTGGCCCACTCGCCAaaga                                                       >  1:1720518/1‑151 (MQ=255)
                                                                                         aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGAAGCG‑CTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGGGATGGGGCGGACTCGCAAgaga                                                       >  2:1498182/1‑151 (MQ=255)
                                                                                         aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTTGGGTGAAAGAAGCG‑CTGAAAAGGAAGGGGGGGGGGGGGTTTGGCCTTCCGGAAGAGAAGGAAAGTCCGGAAGAACCGGATGTggggggccctgaagggg                                                       >  2:1539453/1‑136 (MQ=255)
                                                                                            gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGGTGGGGGGAAAGATGCG‑CTGGAAGGGCCGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGCGCACTCGCCAGAGAAgg                                                    >  1:166783/1‑151 (MQ=255)
                                                                                            gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑C‑GGGTGGGGGGAAAGATGCG‑CTGAAAGGGGCGGGGGGGGGGGGGTTTGTTCTTTCGGAAGGGAGAGACCGGATGGCAAATCGGGGGGGGGGGCACTCCCCAgagagag                                                    >  2:1605467/1‑148 (MQ=255)
                                                                                            gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑C‑GGGTGGGGGGAAAGATGCG‑CCGAAAGGGGAGGGGGGGGTGGCGTTTGTTATTCCGAAAGAGAGCGACCGATTGGGAGATTGGGGTGTGGCGCACTCGCCAGAGAAgg                                                    >  1:1282472/1‑151 (MQ=255)
                                                                                               gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACTCGCCAGAGAAggggg                                                 >  2:838213/1‑148 (MQ=255)
                                                                                                 gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGGGTAAGAAGGG‑CTGGAAAGGGAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGGGACAGTCTGGTAGATCGCGATGTGGCGCCCCCGCAAGAGAAGGGGAtt                                               >  1:1590597/1‑151 (MQ=255)
                                                                                                 gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGATGCG‑CCGGAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGGTGTGGGGCACTCGCAAGAGAAGGCGAtt                                               >  1:1592636/1‑151 (MQ=255)
                                                                                                 gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑G‑GGTTGGGGGGGAAGATGCG‑CTGGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGGGCGACAGGCTGGAAGATCGGGATGTGGCGCACTCGCCAGAGAAGGCGAtt                                               >  1:615314/1‑151 (MQ=255)
                                                                                                     aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                           >  2:1148668/1‑151 (MQ=255)
                                                                                                      tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc                                          <  1:1740472/151‑1 (MQ=255)
                                                                                                      tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGGGGAAGATGCG‑CTGAAAGGGCCGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGGGACAGGCTGGGAGAGCGTGGTGTGGGGCACTCGCAAGAAGAGGCGGTTATggg                                          >  1:889986/1‑150 (MQ=255)
                                                                                                       ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcg                                         <  2:1487626/151‑1 (MQ=255)
                                                                                                       ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGAAAGAAGCG‑CTGAAAGGGGAGGGGGCGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGGCTGGAAGATCGGGGTGTGGCGCCATCGGCAGAGAAGGCGGTTATgggg                                         >  1:1119058/1‑149 (MQ=255)
                                                                                                            gCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGGTGGGGGGGAAGAAGCG‑CTGGAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGGGTATGGCGCcggg                                    >  1:51866/1‑150 (MQ=255)
                                                                                                             ccATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                     <  2:1024997/149‑1 (MQ=255)
                                                                                                               aTCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGCGGGGGGGGTTGTCCTTTCGAAAGAGGGCGAAAGTCTGGAAGATCGGGGTGGGGGGCACCCGCCAGGGAAGGCGGTTATGGCGCcgggggg                                 >  2:1716837/1‑150 (MQ=255)
                                                                                                                        ttGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGa                         <  2:763868/150‑1 (MQ=255)
                                                                                                                             gAAGTGGCGG‑T‑GGGTGGGGGGAAAGAAGCG‑CTGAAAAGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGACAGCCCGATTGGcg                   >  1:749151/1‑150 (MQ=255)
                                                                                                                                      g‑t‑ggtTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAACGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGATGGTGGACAGCCAGATTGGCAACTTTGgc           <  2:1130211/150‑1 (MQ=255)
                                                                                                                                        t‑ggtTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc          <  1:1227878/150‑1 (MQ=255)
                                                                                                                                          ggctGGGGCGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg         >  1:1519920/4‑150 (MQ=255)
                                                                                                                                              ggggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  1:1725573/1‑151 (MQ=255)
                                                                                                                                              ggggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  1:1482332/1‑151 (MQ=255)
                                                                                                                                               gggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  2:623920/1‑150 (MQ=255)
                                                                                                                                                 gTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcg  >  2:528323/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑T‑GGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354144‑354436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑