Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 993,917 | 0 | A | C | 51.9% | 54.3 / 81.1 | 52 | V43G (GTG→GGG) | ssuB | aliphatic sulfonate ABC transporter ATPase |
Reads supporting (aligned to +/- strand): ref base A (22/3); new base C (0/27); total (22/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.50e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-08 |
GTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATG > NC_000913/993768‑994064 | gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCcca > 2:314510/1‑150 (MQ=255) gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCCGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCcca > 2:1575813/1‑150 (MQ=255) cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAGCACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCcaccac < 1:1724026/150‑1 (MQ=255) agccgcgCATCCTGAAACATCATCCGCGTGTCTTCCTGAATTTCACCCAGCGGTGGGGGGCCCGCTAACCC‑‑CGCCGGCGGTTGGCGTTTCCAGCCCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGc < 1:1606216/147‑1 (MQ=255) tCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACaaa > 1:157505/1‑149 (MQ=255) gAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGAc > 1:1428500/1‑150 (MQ=255) atcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCg > 1:112581/1‑151 (MQ=255) tcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg > 2:180408/1‑151 (MQ=255) tcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg > 2:812827/1‑151 (MQ=255) atTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGaa > 2:762470/1‑151 (MQ=255) ttCGCGTGTCTTCCTGAATTTCACCCGGGGGTGGGGGGCCCGCAAACACATCGCGGGGGGTTGGCTTTCCCAGCCCGCCCAGCAGGCGCAGGGGGGTTTTTTT‑CCCC‑CCC‑CCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAat < 2:1477689/151‑1 (MQ=255) tCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAata > 1:973007/1‑151 (MQ=255) ccgctTGTCTCCCTAAATTCGCGCCGGGGGTGTGGTCCCCCCAACCCCTCCGCCTGTGGTGGGCGTTCCCAGCCCGCCCGGCGGGCCCA‑CCGGGTTCTTTTCCCCC‑CCC‑CCCCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAata < 1:320568/150‑1 (MQ=255) cgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt > 1:489984/1‑151 (MQ=255) tcctaaaaTTTAGGCCAGGGGGGGGGGCCCCCCAAACACATCCCCGGGGTTGGGCTTTCCCAGCCCCCCCGGCAGGCGCAGGAGGGTATTTTT‑CCCC‑CCC‑CCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATcc < 2:1350651/145‑1 (MQ=255) ccgggggTGGGGTCCCCGCAAACCCACCCCCGGGGTTGGGCTTTCCCAGCCCTCCCAGCGGGCCCAGGGGGGTTCTTTT‑CCCC‑CCC‑CCCCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGAc < 2:265211/146‑1 (MQ=255) aaggggggtggtgCCCGCTACCACCCGGCCTGGGGTGGTATTTTCCAGCCCGGCCGGGAGCCCCAGAAGGGTATTTTT‑CCCA‑CCC‑CCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGAc < 1:742668/143‑1 (MQ=255) gtgtGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAtt > 2:1326087/1‑151 (MQ=255) gtgtGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAtt > 1:153499/1‑151 (MQ=255) gtgtGGCCCCCGCAACCCCACCGCCTGCGGTGGGCTTTCCCAGCCCCCCCGGCGGGCGCGGCGGGTTTTTTTT‑GCCC‑CCC‑CCGTTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGatat < 1:1132522/150‑1 (MQ=255) cGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa > 1:625688/1‑151 (MQ=255) cTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATg > 2:1656363/1‑151 (MQ=255) cTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATg > 2:197781/1‑151 (MQ=255) cTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATg > 2:1034565/1‑151 (MQ=255) cGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTa > 1:1106276/1‑150 (MQ=255) ctgggtttGGCGTTCCCAGCCCGCCCAGGAGCCGCAGCGGGTTCTTTTT‑CCCC‑CCC‑CCGCTGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGAACGATATTTTCCGCGTAATGTTTGCTTACTGc < 2:649688/145‑1 (MQ=255) gggttggtttccccAGCCCGGCCGGCGGGCGCAGCAGGTTTC‑TTT‑GCCACCCC‑CCGCGGGGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCAt < 2:598103/138‑1 (MQ=255) ttggttttcccAGCCCGGCCAGCGGGCGCGGC‑GGGTTCTTTTCCCCC‑CCC‑CCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAg < 2:1297087/142‑1 (MQ=255) tggctttcacAGCCCTGCCGGACGGCGCAGCGGGGT‑TTTTT‑GACACCCC‑CCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGc < 2:1323243/142‑1 (MQ=255) ttcccaccccTCCCAGCGGGCGCAGCAGGTTTCTTTC‑CCCA‑CCC‑CCTCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaacaa < 1:812409/143‑1 (MQ=255) ttCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAAt > 2:1114368/1‑151 (MQ=255) ccaccccgcccggcgggCGCAGAAGGGTCCTTTG‑CCCC‑CCC‑CCTGTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATgg < 2:1661103/136‑1 (MQ=255) acaCCGGCAAGCGGGCGCAGCGGGGTTTTTTT‑CCCC‑CCC‑CCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg < 2:506060/149‑1 (MQ=255) cccTGCCAGCGGGCGCAGCAGGTTTTTTTT‑CCCC‑CCC‑CCGCTGCGCCCCCCCACCGCCACAAACTGACCTGTCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg < 2:1395544/148‑1 (MQ=255) gcgcAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGa > 2:196784/1‑151 (MQ=255) aCTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCCGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt > 1:229438/1‑150 (MQ=255) aCTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt > 1:142683/1‑150 (MQ=255) ttttt‑GCCC‑CCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc < 2:1258515/150‑1 (MQ=255) tctttcccca‑ccc‑CCGCTGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc < 1:491337/144‑1 (MQ=255) tctttgcccccca‑ccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc < 2:1065653/141‑1 (MQ=255) ttcccccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc < 2:567889/142‑1 (MQ=255) tgcccccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc < 2:490157/142‑1 (MQ=255) tcgccccccgccagccGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc < 1:44618/136‑1 (MQ=255) tccccccccccggggcgcCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc < 2:640713/137‑1 (MQ=255) tccccccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc < 2:1475595/142‑1 (MQ=255) tccccccccccGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc < 2:1497407/142‑1 (MQ=255) tccccccccccGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc < 2:794306/142‑1 (MQ=255) tccccccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc < 1:947298/142‑1 (MQ=255) ccca‑ccc‑CCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCg < 1:1516846/150‑1 (MQ=255) cccccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAcc < 2:1307298/143‑1 (MQ=255) cccccccccGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAcc < 2:353538/143‑1 (MQ=255) cca‑ccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTgg > 2:1468027/1‑150 (MQ=255) gCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctc > 1:658112/1‑149 (MQ=255) gCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCa > 2:247045/1‑151 (MQ=255) ccccACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAt < 2:143773/150‑1 (MQ=255) ccccccccCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATg < 2:620737/151‑1 (MQ=255) ccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATg < 1:914102/151‑1 (MQ=255) | GTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTT‑GCCA‑CCA‑CCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATG > NC_000913/993768‑994064 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |