Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,9170AC51.9% 54.3 / 81.1 52V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (22/3);  new base C (0/27);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 8.50e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-08

GTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATG  >  NC_000913/993768‑994064
                                                                                                                                                        |                                                                                                                                                   
gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCcca                                                                                                                                                     >  2:314510/1‑150 (MQ=255)
gTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCCGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCcca                                                                                                                                                     >  2:1575813/1‑150 (MQ=255)
    cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAGCACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCcaccac                                                                                                                                                 <  1:1724026/150‑1 (MQ=255)
     agccgcgCATCCTGAAACATCATCCGCGTGTCTTCCTGAATTTCACCCAGCGGTGGGGGGCCCGCTAACCC‑‑CGCCGGCGGTTGGCGTTTCCAGCCCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGc                                                                                                                                              <  1:1606216/147‑1 (MQ=255)
              tCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACaaa                                                                                                                                        >  1:157505/1‑149 (MQ=255)
                  gAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGAc                                                                                                                                   >  1:1428500/1‑150 (MQ=255)
                       atcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCg                                                                                                                             >  1:112581/1‑151 (MQ=255)
                        tcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg                                                                                                                            >  2:180408/1‑151 (MQ=255)
                        tcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg                                                                                                                            >  2:812827/1‑151 (MQ=255)
                          atTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGaa                                                                                                                          >  2:762470/1‑151 (MQ=255)
                           ttCGCGTGTCTTCCTGAATTTCACCCGGGGGTGGGGGGCCCGCAAACACATCGCGGGGGGTTGGCTTTCCCAGCCCGCCCAGCAGGCGCAGGGGGGTTTTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAat                                                                                                                         <  2:1477689/151‑1 (MQ=255)
                            tCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAata                                                                                                                        >  1:973007/1‑151 (MQ=255)
                            ccgctTGTCTCCCTAAATTCGCGCCGGGGGTGTGGTCCCCCCAACCCCTCCGCCTGTGGTGGGCGTTCCCAGCCCGCCCGGCGGGCCCACCGGGTTCTTTTCCCCCCCCCCCCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAata                                                                                                                        <  1:320568/150‑1 (MQ=255)
                               cgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                     >  1:489984/1‑151 (MQ=255)
                                     tcctaaaaTTTAGGCCAGGGGGGGGGGCCCCCCAAACACATCCCCGGGGTTGGGCTTTCCCAGCCCCCCCGGCAGGCGCAGGAGGGTATTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATcc                                                                                                               <  2:1350651/145‑1 (MQ=255)
                                                   ccgggggTGGGGTCCCCGCAAACCCACCCCCGGGGTTGGGCTTTCCCAGCCCTCCCAGCGGGCCCAGGGGGGTTCTTTTCCCCCCCCCCCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGAc                                                                                                 <  2:265211/146‑1 (MQ=255)
                                                    aaggggggtggtgCCCGCTACCACCCGGCCTGGGGTGGTATTTTCCAGCCCGGCCGGGAGCCCCAGAAGGGTATTTTTCCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGAc                                                                                                 <  1:742668/143‑1 (MQ=255)
                                                         gtgtGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAtt                                                                                           >  2:1326087/1‑151 (MQ=255)
                                                         gtgtGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAtt                                                                                           >  1:153499/1‑151 (MQ=255)
                                                         gtgtGGCCCCCGCAACCCCACCGCCTGCGGTGGGCTTTCCCAGCCCCCCCGGCGGGCGCGGCGGGTTTTTTTTGCCCCCCCCGTTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGatat                                                                                            <  1:1132522/150‑1 (MQ=255)
                                                                   cGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa                                                                                 >  1:625688/1‑151 (MQ=255)
                                                                     cTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATg                                                                               >  2:1656363/1‑151 (MQ=255)
                                                                     cTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATg                                                                               >  2:197781/1‑151 (MQ=255)
                                                                     cTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATg                                                                               >  2:1034565/1‑151 (MQ=255)
                                                                              cGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTa                                                                       >  1:1106276/1‑150 (MQ=255)
                                                                                 ctgggtttGGCGTTCCCAGCCCGCCCAGGAGCCGCAGCGGGTTCTTTTTCCCCCCCCCGCTGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGAACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                                   <  2:649688/145‑1 (MQ=255)
                                                                                    gggttggtttccccAGCCCGGCCGGCGGGCGCAGCAGGTTTCTTTGCCACCCCCCGCGGGGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCAt                                                                 <  2:598103/138‑1 (MQ=255)
                                                                                       ttggttttcccAGCCCGGCCAGCGGGCGCGGCGGGTTCTTTTCCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAg                                                             <  2:1297087/142‑1 (MQ=255)
                                                                                        tggctttcacAGCCCTGCCGGACGGCGCAGCGGGGTTTTTTGACACCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGc                                                            <  2:1323243/142‑1 (MQ=255)
                                                                                             ttcccaccccTCCCAGCGGGCGCAGCAGGTTTCTTTCCCCACCCCCTCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaacaa                                                       <  1:812409/143‑1 (MQ=255)
                                                                                              ttCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAAt                                                      >  2:1114368/1‑151 (MQ=255)
                                                                                                ccaccccgcccggcgggCGCAGAAGGGTCCTTTGCCCCCCCCCTGTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATgg                                                    <  2:1661103/136‑1 (MQ=255)
                                                                                                  acaCCGGCAAGCGGGCGCAGCGGGGTTTTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg                                                  <  2:506060/149‑1 (MQ=255)
                                                                                                    cccTGCCAGCGGGCGCAGCAGGTTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGTCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg                                                  <  2:1395544/148‑1 (MQ=255)
                                                                                                                gcgcAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGa                                    >  2:196784/1‑151 (MQ=255)
                                                                                                                            aCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCCGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt                         >  1:229438/1‑150 (MQ=255)
                                                                                                                            aCTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt                         >  1:142683/1‑150 (MQ=255)
                                                                                                                             tttttGCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc                        <  2:1258515/150‑1 (MQ=255)
                                                                                                                             tctttccccacccCCGCTGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc                        <  1:491337/144‑1 (MQ=255)
                                                                                                                              tctttgccccccaccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTc                        <  2:1065653/141‑1 (MQ=255)
                                                                                                                                   ttcccccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                    <  2:567889/142‑1 (MQ=255)
                                                                                                                                   tgcccccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                    <  2:490157/142‑1 (MQ=255)
                                                                                                                                   tcgccccccgccagccGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                    <  1:44618/136‑1 (MQ=255)
                                                                                                                                   tccccccccccggggcgcCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                    <  2:640713/137‑1 (MQ=255)
                                                                                                                                   tccccccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                    <  2:1475595/142‑1 (MQ=255)
                                                                                                                                   tccccccccccGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                    <  2:1497407/142‑1 (MQ=255)
                                                                                                                                   tccccccccccGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                    <  2:794306/142‑1 (MQ=255)
                                                                                                                                   tccccccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAc                    <  1:947298/142‑1 (MQ=255)
                                                                                                                                   cccacccCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCg                  <  1:1516846/150‑1 (MQ=255)
                                                                                                                                     cccccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAcc                   <  2:1307298/143‑1 (MQ=255)
                                                                                                                                     cccccccccGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATAcc                   <  2:353538/143‑1 (MQ=255)
                                                                                                                                        ccaccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTgg               >  2:1468027/1‑150 (MQ=255)
                                                                                                                                              gCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctc           >  1:658112/1‑149 (MQ=255)
                                                                                                                                                 gCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCa      >  2:247045/1‑151 (MQ=255)
                                                                                                                                                    ccccACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAt   <  2:143773/150‑1 (MQ=255)
                                                                                                                                                     ccccccccCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATg  <  2:620737/151‑1 (MQ=255)
                                                                                                                                                     ccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATg  <  1:914102/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                   
GTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATG  >  NC_000913/993768‑994064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: