Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA66.1% 92.2 / 112.4 121intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (7/34);  new base A (80/0);  total (87/34)
Fisher's exact test for biased strand distribution p-value = 1.73e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-13

Only 100 of 121 total aligned reads displayed.

CTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTT  >  NC_000913/2386716‑2387010
                                                                                                                                                     |                                                                                                                                                                  
cTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGAc                                                                                                                                                                <  1:615411/151‑1 (MQ=255)
  ttttATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCGAAAAAAAGACaa                                                                                                                                                              >  1:625204/1‑150 (MQ=255)
   tttATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATg                                                                                                                                                             <  2:1143185/151‑1 (MQ=255)
         gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTTCCAAAAAAAG‑GACATTAAC‑att                                                                                                                                                      >  2:484774/1‑151 (MQ=255)
         gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCAGAAAAAAAGACAAGgac‑aat                                                                                                                                                      >  2:1721003/1‑149 (MQ=255)
         gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAACGACATAcaacat                                                                                                                                                       >  1:1733441/1‑147 (MQ=255)
         gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAAAGAAAAGCAC‑att                                                                                                                                                      >  2:333447/1‑151 (MQ=255)
         gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GTCCGAAAAAAAGACAAACAC‑att                                                                                                                                                      >  1:515186/1‑151 (MQ=255)
         gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCCGAAAAAAAGACATGCAC‑att                                                                                                                                                      >  2:347581/1‑151 (MQ=255)
              aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAGAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAAAGACAAGCAA‑attttttt                                                                                                                                                 >  1:438778/1‑151 (MQ=255)
              aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCAAAAAAAAAGACaatgccaatttttt                                                                                                                                                  >  1:1647370/1‑138 (MQ=255)
              aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TGCCCAAAAAAAGACAAGCAC‑attttatt                                                                                                                                                 >  1:723279/1‑151 (MQ=255)
              aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GTCAAAAAAAGAGACAAGGCC‑actttatt                                                                                                                                                 >  1:1041411/1‑151 (MQ=255)
                 cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCGAAAAAAAGACCAGCAC‑ccttttttttt                                                                                                                                              >  1:723903/1‑150 (MQ=255)
                 cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCAGAAAAAAAGACAAGCAC‑aattttttttt                                                                                                                                              >  1:304699/1‑150 (MQ=255)
                 cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TGCCGAAAAAAAGACAAGCAC‑atttttttttt                                                                                                                                              >  1:1270143/1‑150 (MQ=255)
                 cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAAAGACAAGCAC‑aattttttttt                                                                                                                                              >  2:835602/1‑150 (MQ=255)
                 cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAACGACAAGGAC‑atttttttttt                                                                                                                                              >  2:1700251/1‑150 (MQ=255)
                    tACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCt                                                                                                                                           <  1:1065716/151‑1 (MQ=255)
                        aCTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATc                                                                                                                                      <  1:661988/151‑1 (MQ=255)
                               cGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTa                                                                                                                            <  2:1086149/151‑1 (MQ=255)
                                 gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCGGAAAAAAGAAAAAGAC‑AATTTATTTTTTCT‑CATC‑T‑G‑tttttta                                                                                                                          >  1:1047384/1‑148 (MQ=255)
                                 gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAAAGACATGCAC‑ATTTTTTTTTTCCT‑CCTT‑C‑G‑tttttaa                                                                                                                          >  1:382935/1‑148 (MQ=255)
                                 gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAAAGAAAAGCAA‑ATTTTTTTTTTCCT‑TATC‑T‑G‑TTTTAAg                                                                                                                          >  2:669166/1‑151 (MQ=255)
                                      aCCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAAAGGAAAGCAC‑ATTTTTTTTTTCCC‑CCTC‑t‑t‑tttttaaaaggg                                                                                                                     >  2:1721729/1‑143 (MQ=255)
                                            gacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCCGAAAAAAAGAAAAGGAC‑ATTTTTTTTTTCCT‑CATC‑t‑t‑tttttaaaagggaaaaat                                                                                                               >  2:1371814/1‑137 (MQ=255)
                                              cagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAAAGACAAGGAC‑ATTTTTTTTTTCCT‑CCTC‑T‑T‑TTTTTAAATACG‑AAACAA‑G‑a                                                                                                          >  2:614859/1‑151 (MQ=255)
                                              cagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GTCCGAAAAAAAGACATGCAC‑ATTTTATTTTTTCT‑CTTC‑T‑G‑TTTTTAGATTCG‑AAAAAT‑G‑a                                                                                                          >  1:854863/1‑151 (MQ=255)
                                                gaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAAAAAAAAACAC‑ATTTTTTTTTTTCT‑CATC‑T‑G‑TTTTTAGAATCG‑AAAAAA‑G‑aaa                                                                                                        >  1:532742/1‑151 (MQ=255)
                                                gaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAAAGACAATCAA‑AATTTATTTTTCCT‑CCTC‑T‑G‑TTTTTAAATggagaaaaataaa                                                                                                           >  2:1703947/1‑139 (MQ=255)
                                                gaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAAAGACAAACAC‑ATTTTTTTTTTTCC‑CCTC‑T‑T‑TTTTTAGATGCG‑gaaaaaaaaa                                                                                                          >  2:50733/1‑144 (MQ=255)
                                                 aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAACGAAAAGCAC‑CTTTTTTTTTTCCC‑CCTC‑T‑T‑TTTTTAGATGCG‑gaaaaaaaaat                                                                                                         >  2:728087/1‑143 (MQ=255)
                                                         aTCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAACGGCAAGCAC‑ATTTTTTTTTTCCT‑CATC‑T‑T‑TTTTTAAAAGCG‑GAAAAA‑G‑aaaattaaaggg                                                                                               >  2:116644/1‑142 (MQ=255)
                                                         aTCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAACGACATGCAC‑ATTTTTTTTTTTCT‑CATC‑T‑T‑TTTTTAGATGAG‑GAAAAT‑G‑AAATTC‑A‑Acagg                                                                                             >  1:1049002/1‑148 (MQ=255)
                                                              aaaaTGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGaa                                                                                       <  1:50716/150‑1 (MQ=255)
                                                                    gCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCGAAAAAAAGCAAAGACC‑ATTTTTTTTTTTCT‑CATC‑T‑G‑TTTTTAGATACG‑GAAACT‑G‑AAATTCAA‑AC‑GGG‑GGaaaaaa‑aa                                                                                >  1:1408933/1‑151 (MQ=255)
                                                                     cTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAaaat‑aaa                                                                               <  1:1157761/150‑1 (MQ=255)
                                                                       cAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCGAAAAAACGACAAGCAC‑CTTTTTTTTTTCCC‑CATC‑T‑T‑TTTTTAAATGCG‑GAAAAA‑G‑AAAATT‑A‑AA‑GGG‑GGAAAAAA‑AAAAgg                                                                            >  2:1387195/1‑151 (MQ=255)
                                                                         ggTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGG‑TCCA‑AAAAAAAGACAAAGAC‑AATTTTTTTTTTCT‑CCTC‑T‑G‑TTTTTAAATACG‑AAAAAA‑G‑AAATTT‑A‑AC‑AGAGGGAAAAAA‑AAATggg                                                                           >  1:1313303/1‑151 (MQ=255)
                                                                         ggTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCGAAAAAAAGAAAAGCAC‑ATTTTTTTTTTTCT‑CATC‑T‑G‑TTTTTATATGCG‑GAAAAA‑G‑AAAATT‑A‑AA‑GGA‑GGGAAAAA‑AAATtggg                                                                          >  1:452300/1‑150 (MQ=255)
                                                                         ggTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCAGAAAAAACGACATGCCA‑AATTTTTTTTTCCT‑CCTC‑T‑T‑TTTTTATAAAGG‑GAAAAA‑G‑AAAATT‑A‑AA‑GGA‑GGGAAAAA‑AAAAgggg                                                                          >  2:260239/1‑150 (MQ=255)
                                                                         ggTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCAGAAAAAAAGAAAAGAAA‑ATTTTTTTTTTTCT‑TCTC‑T‑T‑TTTTTTAAAGCGAAAAAAT‑A‑AAATAA‑A‑AC‑GGG‑GGAAAAAA‑AAATggg                                                                           >  1:1188405/1‑151 (MQ=255)
                                                                         ggTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GTCAGAAAAAAAGACAATGAC‑ATTTTTTTTTTTCT‑TCTC‑T‑G‑GTTTTAAATGCG‑AAACAA‑G‑AAAATC‑A‑AA‑GGG‑GGGAAAAT‑AAATgggg                                                                          >  1:82968/1‑150 (MQ=255)
                                                                         ggTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCCGAAAAAAAGACAAGCAC‑ATTTTTTTTTTCCT‑CCTC‑T‑G‑TTTTTAGAAGCG‑GAAAAA‑G‑AAATTT‑A‑AC‑GGG‑GGGAAAAA‑AAATgggg                                                                          >  2:739501/1‑150 (MQ=255)
                                                                         ggTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCCAAAAAAACGACAAGCAC‑AATTTTTTTTTTCT‑CCTC‑T‑T‑TTTTTAAAAGGG‑GAAAAT‑A‑AAAATT‑A‑AA‑GGG‑GGGAAAAA‑AAATGGGa                                                                          >  2:852267/1‑151 (MQ=255)
                                                                          gTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATgg                                                                            <  2:1005749/148‑1 (MQ=255)
                                                                           tAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAAt                                                                        <  2:410896/151‑1 (MQ=255)
                                                                             gTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCAGAAAAAACGCAAACAAC‑AATTTTTTTTTCCT‑CCTC‑T‑G‑GTTTTAGAAGGG‑GAAAAA‑G‑AAAAAT‑A‑AA‑AGA‑GGAaaaaa‑aaaatggggaga                                                                      >  2:672416/1‑142 (MQ=255)
                                                                              tGGATGGTGTTATAACTTTGTTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATg                                                                       <  2:117370/150‑1 (MQ=255)
                                                                              tGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGa                                                                      <  1:1479813/150‑1 (MQ=255)
                                                                                gATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑tt                                                                   <  2:1418877/150‑1 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCGAAAAAAAGAAAAAAAC‑ATTTTTTTTTTCCT‑CCTC‑T‑T‑TTTTTAAATGCG‑AAAAAA‑T‑AAATTT‑A‑AC‑GGG‑GGAAAAAA‑AAAAAGGGAAGA‑TTTGCTa                                                              >  2:1277275/1‑151 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCCAAAAAAAAAAAAACAC‑ATTTTTTTTTTTCT‑CCTC‑T‑G‑TTTTTAAAAACG‑GAAAAT‑GAAAATTT‑A‑AC‑GG‑‑GGAAAAAA‑AAAAGGGGATGA‑tttggca                                                              >  1:760449/1‑146 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCAGAAAAAACGAACAGGAC‑ATTTTTTTTTTCCTCCTTT‑T‑T‑TTTTAAAATG‑G‑AAAAAA‑A‑AAAATC‑A‑AC‑GGA‑GGGAAAATAAAATGGGAAGGT‑TTggtt                                                               >  2:776705/1‑149 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAACGACAATTAC‑ATTTTATTTTTCCT‑CATC‑T‑G‑TTTTTAAATGCG‑CAAAAT‑G‑AAAATT‑A‑AA‑GGA‑GGAAAAAT‑AAATGGGAAAGA‑tttggta                                                              >  2:1529155/1‑146 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAAACACATTTAC‑AATTTTTTTTTCCC‑CAAC‑T‑G‑TTTTTAGATGGGAAAAAAA‑A‑AAATTA‑A‑AC‑GCG‑GGAAAAA‑‑AAATGGGAATGA‑ATGGCTa                                                              >  2:599954/1‑151 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCAAAAAAAAGACATGCAC‑ATTTTTTTTTTTCT‑CCTC‑T‑T‑TTTTTAAATGCG‑AAAAAT‑G‑AAAATT‑A‑AA‑GGA‑GGGAAAAA‑AAATAGGAAATA‑TTGGGTa                                                              >  1:1178027/1‑151 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCt                                                               <  2:465404/150‑1 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAACGAAAAGCAC‑ATTTTATTTTTCCT‑CATC‑T‑T‑TTTTTAAAAGCG‑AAAAAA‑A‑AAAATT‑A‑ACGGGG‑GGAAAAAA‑AAATGGG‑ATGA‑TTTGCtt                                                              >  2:197227/1‑150 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAAAGACAAGCAC‑ATTTTTTTTTTCCC‑CCTC‑T‑G‑TTTTTATAATCG‑GAAAAA‑G‑AAATTC‑A‑AA‑GGA‑GGAAAAAA‑AAAAGGGGAAGA‑TTgggtt                                                              >  2:242336/1‑148 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GTCAGAAAAAAAGAAATTCACAATTTTTTTTTCCCT‑C‑TT‑T‑G‑TTTTAAGAATCG‑AAAAAT‑GAAAATTA‑A‑AG‑GGG‑GGAAAAAA‑AAATGGGatgaatgggtt                                                                >  1:1257792/1‑141 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GTCAGAAAAAAAGAAAAGCAA‑ATTTTATTTTTTCT‑CATC‑TGG‑TTTTAAAAGGGG‑AAAAAT‑A‑AAATT‑‑A‑AA‑AGA‑GGAAAAAT‑AAATGGGGATGA‑TTggggt                                                              >  1:991024/1‑148 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCCGAAAAAAAGACAAGGAC‑ATTTTATTTTTCCT‑CCTC‑T‑G‑TTTTTAGAAGCG‑GAAAAT‑G‑AAAATC‑A‑AA‑GGA‑GGAAAAAT‑AAAATGGGATGA‑tttggta                                                              >  1:53815/1‑146 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAACGACATGCCC‑ATTTTTTTTTTCCC‑CATC‑T‑G‑TTTTTAGATGCG‑GAAAAA‑G‑AAAATT‑A‑AA‑GGG‑GGGAAAAA‑AAATGGGGATGA‑tttggta                                                              >  2:1024842/1‑146 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAACGACATGCAC‑ATTTTTTTTTTCCT‑CCTC‑T‑G‑TTTTTAGAAGCG‑GAACAT‑G‑AAATTC‑A‑AC‑GGG‑GGGAAAAA‑AAATGGGGATGA‑TTGGCTa                                                              >  2:110580/1‑151 (MQ=255)
                                                                                    gtgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAAAAACAAGGAA‑AATTTTTTTTTCCC‑CATC‑T‑T‑TTTTTAAAAGGG‑AAAAAA‑G‑AAAATT‑A‑AA‑GGA‑GGGAAAAA‑AAAAGGGGAAGA‑tttggtt                                                              >  2:1629784/1‑146 (MQ=255)
                                                                                     tgtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTa                                                              <  2:1178425/150‑1 (MQ=255)
                                                                                      gtTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TTCCGAAAAAAAGACAAACCA‑AATTTATTTTTTCT‑CCTC‑T‑G‑TTTTTAAATAAG‑AAACAT‑G‑AAATTC‑A‑AC‑CGA‑GGGAAAAA‑AAATTGTGATGA‑TTGGGTAtt                                                            >  1:1182684/1‑151 (MQ=255)
                                                                                        tataACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTAtttt                                                          <  2:625458/151‑1 (MQ=255)
                                                                                               ttG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GTCCGAAAAAAAAAAAAGCAA‑ATTTTTTTTTCCCT‑CCTC‑C‑G‑TTTTTATATTCG‑GAAAATAA‑AAATTA‑A‑AC‑GGG‑GGAAAAAA‑AAATGGG‑ATGA‑TTTGCTAATTTTCACaaa                                                   >  2:789351/1‑151 (MQ=255)
                                                                                                 g‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAAGGACATGCAC‑ATTTTTTTTTTCCT‑CATC‑T‑GTTTTTAAAAGGGG‑AAAAAA‑A‑AAATT‑‑A‑AA‑GGA‑GGGAAAAT‑AAATTGGGATGA‑TTGGGttttttttcaaaaat                                                 >  2:1688894/1‑142 (MQ=255)
                                                                                                 g‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑CCCCGAAAAAAAACAAAAAAC‑AATTTATTTTTCCC‑CCTC‑T‑C‑TTTTTAGAAGAG‑AAAAAA‑A‑AAAATT‑A‑AA‑GGG‑GGAAAAAA‑AAATTGTGAAGA‑TTGGGTATTTTTTAGaaaaa                                                 >  1:841900/1‑149 (MQ=255)
                                                                                                     ataCAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCAAAAAAAAGACAAGCAA‑AATTTATTTTTTCT‑CATC‑T‑G‑TTTTTAGATGCG‑AAACAT‑G‑AAAATT‑A‑AC‑GGA‑GGGAAAAT‑AAATGGGAATGA‑TTGGGTATTTTTCAGaaaattaa                                              >  1:554149/1‑146 (MQ=255)
                                                                                                     ataCAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATTA‑TTGGCTATTTTGCAGAAATTGAc                                              <  1:612228/151‑1 (MQ=255)
                                                                                                       acaacaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTc                                            <  1:545213/151‑1 (MQ=255)
                                                                                                        caacaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCCGAAAAAACGACATGCCC‑CTTTTTTTTTTCCC‑CCTC‑C‑T‑TTTTTAAATGCG‑AAAAAT‑G‑AAATTC‑A‑AC‑GGG‑GGAAAAAT‑AAAATGGGATGG‑TTGGGTATTTTTGAGaaaattaatca                                           >  2:45600/1‑143 (MQ=255)
                                                                                                        caacaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTTCCT‑CCTC‑T‑G‑TTTTAAGATGCG‑AAACAA‑G‑AAATTC‑A‑AA‑GGA‑GGAAAAAT‑AAATGGGAATGT‑TTTGCTATTTTTTAGAAATTAAccta                                           >  2:414174/1‑148 (MQ=255)
                                                                                                        caacaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAAAGACAAACAC‑ATTTTATTTTTTCC‑CTTCTT‑G‑TTTTAA‑AAGCG‑GAAAAA‑G‑AAAATC‑T‑AA‑GGAGGGAAAAAA‑AAAAGGGAAGAA‑TTGG‑TAttttttcgaaaatgaacta                                           >  1:1166955/1‑136 (MQ=255)
                                                                                                         aacaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GGCAAAAAAAAGGAAAAGCAC‑ATTTTATTTTTCCT‑CCTC‑T‑G‑TTTTTAGATGCG‑GAACAA‑G‑AAAATT‑A‑AA‑CGA‑GGGGAAAA‑AAATAGGGATGA‑TTTGGTATTTTTTAGAAAATTACTCAg                                          >  1:1613238/1‑151 (MQ=255)
                                                                                                          acaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAACGACACGCAC‑ATTTTTTTTTTCCC‑CCCC‑T‑T‑TTTTTAGATGGG‑AAAAAA‑A‑AAAATT‑A‑AA‑GGA‑GGAAAAAA‑AAAATGGAAAGA‑TTTGGTTTTTTTCAGAAAATTACTCaaa                                         >  2:1535162/1‑149 (MQ=255)
                                                                                                          acaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GTCAGAAAAAAAGAAAAGAAC‑AATTTATTTTTCCC‑CATC‑T‑G‑TTTTTAGAAGCG‑AAAAAT‑G‑AAAATT‑A‑AC‑GGA‑GGAAAAAA‑AAATTGGTATGATTTGGTAATTTTGTAGAAAtgacatcaa                                          >  1:129857/1‑143 (MQ=255)
                                                                                                          acaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑GCCAGAAAAAAAGACAAGCAC‑ATTTTTTTTTTTCC‑CATC‑T‑G‑TTTTTAAAAGGG‑AAAAAT‑G‑AAAATT‑A‑AA‑GGA‑GGAAAAAA‑AAAAGGGGATAA‑TTGGGTATTTTGTAGaaaattagttcga                                         >  1:1519712/1‑141 (MQ=255)
                                                                                                          acaGTAAATAATAAAAATTTCTCGAGGC‑GGG‑CTCCGAAAAAAAGACAAGCAA‑ATTTTTTTTTTTCC‑CTTC‑T‑T‑TTTTTATATGGG‑AAACCA‑G‑CAAATC‑A‑AC‑GGG‑GGGAAAAT‑TAATGGGAATGA‑TTGGGTTTTTTTTAGAAACTGAccccga                                         >  1:240055/1‑146 (MQ=255)
                                                                                                           caGTAAATAATAAAAATTTCTCGAGGC‑GGG‑CACAGAAAAAAACAAAAGATC‑ATTTTTTTTTTTCT‑TTAC‑T‑G‑GTTTTAGAAGAG‑GAACAA‑T‑AAAATC‑ACAA‑GGG‑GGAAAAA‑‑AAAAGGGGATGA‑TTGGGTATTTTGCATAAAATGACATAGaa                                        >  1:589434/1‑151 (MQ=255)
                                                                                                               aaaTAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGa                                    <  2:1550047/151‑1 (MQ=255)
                                                                                                                aataatAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGAt                                   <  2:903161/151‑1 (MQ=255)
                                                                                                                     taaAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAAcc                              <  2:259341/151‑1 (MQ=255)
                                                                                                                     taaAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAAcc                              <  2:1230660/151‑1 (MQ=255)
                                                                                                                      aaaaaTTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCa                             <  2:275522/151‑1 (MQ=255)
                                                                                                                           tttCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGc                        <  1:1024795/151‑1 (MQ=255)
                                                                                                                                cGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAg                   <  2:926201/151‑1 (MQ=255)
                                                                                                                                    gC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCt                <  1:534836/150‑1 (MQ=255)
                                                                                                                                        gg‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTa               >  2:748310/1‑148 (MQ=255)
                                                                                                                                            ccAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaa         <  2:1500924/151‑1 (MQ=255)
                                                                                                                                            ccAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaa         <  1:739145/151‑1 (MQ=255)
                                                                                                                                            ccAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaa         <  1:1243150/151‑1 (MQ=255)
                                                                                                                                               gAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaaaa       >  1:812550/1‑150 (MQ=255)
                                                                                                                                                aaaaaGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAAcc     <  2:456592/151‑1 (MQ=255)
                                                                                                                                                aaaaaGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAAcc     <  1:827227/151‑1 (MQ=255)
                                                                                                                                                   aaGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCttt  >  1:190467/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                                  
CTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTG‑TTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGG‑TCCAGAAAAAGCGACATGCAC‑ATTTTATTTTCCCT‑CATC‑T‑G‑TTTTAAGATGCG‑AAACAT‑G‑AAATTC‑A‑AC‑GGA‑GGAAAAAT‑AAATGGGAATGA‑TTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTT  >  NC_000913/2386716‑2387010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑