Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	894,155	T→G	D124E (GAT→GAG)	potF →	putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	894,155	0	T	G	100.0%	320.5 / NA	92	D124E (GAT→GAG)	potF	putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/67);  total (25/67)

GTACCGGCTTTGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGAAAAT  >  NC_000913/894010‑894287                                                                                                                                                  |                                                                                                                                     gTACCGGCTTTGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGa                                                                                                                                 >  1:355589/1‑151 (MQ=255)      ggCTTTGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATa                                                                                                                            >  1:717807/1‑151 (MQ=255)      ggCTTTGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATa                                                                                                                            >  1:1447931/1‑151 (MQ=255)       gCTTTGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATaa                                                                                                                           >  2:1550538/1‑151 (MQ=255)       gCTTTGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATaa                                                                                                                           >  1:1692974/1‑151 (MQ=255)         tttGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAt                                                                                                                         <  2:1291183/151‑1 (MQ=255)          ttGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAtt                                                                                                                        <  1:648147/151‑1 (MQ=255)            gATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTg                                                                                                                      >  2:1676879/1‑151 (MQ=255)            gATCTGGCGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAACTGGTCGCCAAACACGAGCCCGACAATAAATTTg                                                                                                                      >  2:882285/1‑151 (MQ=255)             aTCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTg                                                                                                                      <  1:766076/150‑1 (MQ=255)             aTCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGc                                                                                                                     <  2:569257/151‑1 (MQ=255)               cTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTa                                                                                                                   <  2:317888/151‑1 (MQ=255)               cTGGTGGTTCCATCTGCCAGCTTTCGGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTa                                                                                                                   <  1:186284/151‑1 (MQ=255)                tggtggTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTAt                                                                                                                  >  2:1383019/1‑151 (MQ=255)                    ggtTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGcc                                                                                                               >  2:1487091/1‑150 (MQ=255)                     gtTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGccc                                                                                                              <  1:1285256/150‑1 (MQ=255)                     gtTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCAAAACACGAGCCCGACAATAAATTTGCTATGccc                                                                                                              <  2:1185560/150‑1 (MQ=255)                         cATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTatat                                                                                                         <  2:1588635/151‑1 (MQ=255)                         cATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTatat                                                                                                         <  1:304976/151‑1 (MQ=255)                         cATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTatat                                                                                                         <  1:179894/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  1:672451/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  1:687359/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  1:553953/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  1:1038141/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  2:1423511/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  2:991954/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  1:1298478/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  2:1684828/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  2:513821/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  2:25378/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  1:1549805/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  2:493410/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  2:734919/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  2:355708/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  1:1634265/151‑1 (MQ=255)                          aTCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTGCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                                                        <  1:230227/151‑1 (MQ=255)                           tCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgt                                                                                                       >  2:1477029/1‑151 (MQ=255)                            cTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgtg                                                                                                      <  2:1179339/151‑1 (MQ=255)                            cTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgtg                                                                                                      <  1:426942/151‑1 (MQ=255)                            cTGCCAGCTTTCTGGAGCGCCAGTTGACGGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgtg                                                                                                      <  1:209764/151‑1 (MQ=255)                             tGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTgg                                                                                                     <  2:1544325/151‑1 (MQ=255)                               ccAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTggg                                                                                                    <  2:98307/150‑1 (MQ=255)                               ccAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTggg                                                                                                    <  2:1330020/150‑1 (MQ=255)                                              gcCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTgg                                                                                    >  2:140271/1‑151 (MQ=255)                                                 aGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                                                                 >  2:1052403/1‑151 (MQ=255)                                                 aGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                                                                 >  1:592724/1‑151 (MQ=255)                                                   ttGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                                                                 <  2:1556464/149‑1 (MQ=255)                                                   ttGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                                                                  <  2:1287680/148‑1 (MQ=255)                                                   ttGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                                                                  <  1:109604/148‑1 (MQ=255)                                                      aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAc                                                                             <  2:774310/150‑1 (MQ=255)                                                      aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAc                                                                             <  1:964324/150‑1 (MQ=255)                                                      aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAc                                                                             <  2:1448586/150‑1 (MQ=255)                                                      aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAc                                                                             <  1:1363683/150‑1 (MQ=255)                                                      aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAc                                                                             <  2:235824/150‑1 (MQ=255)                                                      aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACg                                                                            <  2:360319/151‑1 (MQ=255)                                                       cTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGt                                                                           <  1:1004798/151‑1 (MQ=255)                                                          cGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGa                                                                        >  1:314835/1‑151 (MQ=255)                                                              aGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATaa                                                                     <  1:1373536/150‑1 (MQ=255)                                                                ttttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAg                                                                   <  1:817785/150‑1 (MQ=255)                                                                        ccGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGc                                                            <  1:94354/149‑1 (MQ=255)                                                                        ccGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGc                                                            <  2:191910/149‑1 (MQ=255)                                                                        ccGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGc                                                            <  1:849238/149‑1 (MQ=255)                                                                        ccGCTGGACAAAAGCAAATGGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGCCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAg                                                             <  2:580922/148‑1 (MQ=255)                                                                             ggACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTg                                                        >  2:166637/1‑148 (MQ=255)                                                                             ggACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTg                                                        >  2:354721/1‑148 (MQ=255)                                                                               aCAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTggg                                                   <  1:1682111/151‑1 (MQ=255)                                                                                cAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc                                                  <  1:907064/151‑1 (MQ=255)                                                                                cAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc                                                  <  1:1250920/151‑1 (MQ=255)                                                                                cAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc                                                  <  2:640401/151‑1 (MQ=255)                                                                                 aaaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCg                                                 <  2:1597629/151‑1 (MQ=255)                                                                                      cAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaaaa                                             <  2:347931/150‑1 (MQ=255)                                                                                       aaaTTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcg                                           <  2:192077/151‑1 (MQ=255)                                                                                                  gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGaa                                >  1:1716024/1‑150 (MQ=255)                                                                                                  gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGAt                                >  2:1735128/1‑151 (MQ=255)                                                                                                  gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGGGCTGGGCGAAAACGCGCCCGCCGAt                                >  1:364750/1‑151 (MQ=255)                                                                                                      aagaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGc                             <  2:1751389/150‑1 (MQ=255)                                                                                                         aaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTg                           <  2:718159/149‑1 (MQ=255)                                                                                                                aCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                                                                  >  1:1265516/1‑87 (MQ=255)                                                                                                                aCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                                                                  <  2:1266093/87‑1 (MQ=255)                                                                                                                aCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGAt                  <  1:1589525/151‑1 (MQ=255)                                                                                                                aCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGAt                  <  1:377453/151‑1 (MQ=255)                                                                                                                     gAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTc              <  2:1511985/150‑1 (MQ=255)                                                                                                                     gAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTc              <  2:67316/150‑1 (MQ=255)                                                                                                                     gAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTc              <  1:680174/150‑1 (MQ=255)                                                                                                                         tgctgcAGCTGGTCGCCAAACAAGAGCCCGACAATAAATTTGCCATGCCCTATATGTGGGAGACGACAGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGAGAAAACGCGACAGTCGGTAGATGGGACTTGATCCTCAAAc          >  2:1048825/1‑150 (MQ=255)                                                                                                                         tgctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAAcc         <  2:201930/151‑1 (MQ=255)                                                                                                                         tgctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAAcc         <  1:34902/151‑1 (MQ=255)                                                                                                                         tgctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAGGTGGGCGACGGCCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTAAAAcc         <  2:70976/151‑1 (MQ=255)                                                                                                                          gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAAc          >  2:327238/1‑149 (MQ=255)                                                                                                                          gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAAc          >  2:1516076/1‑149 (MQ=255)                                                                                                                               aGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGaaaa   >  2:1061924/1‑151 (MQ=255)                                                                                                                                   ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGAAAAt  >  2:454816/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                     GTACCGGCTTTGATCTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGAAAAT  >  NC_000913/894010‑894287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑