Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,191,432 C→T G72S (GGC→AGC)  yehC ← putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,191,4320CT100.0% 367.6 / NA 92G72S (GGC→AGC) yehCputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/57);  total (35/57)

GATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGAAAAAATGA  >  NC_000913/2191285‑2191578
                                                                                                                                                   |                                                                                                                                                  
gATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtat                                                                                                                                                  >  2:1134737/1‑150 (MQ=255)
gATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCtat                                                                                                                                                  >  1:989514/1‑150 (MQ=255)
   aaaaaaTGCTTTCTTTAGTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTatcatc                                                                                                                                              <  1:195472/151‑1 (MQ=255)
    aaaaaTGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTAtcatca                                                                                                                                             >  1:915389/1‑151 (MQ=255)
        aTGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATcc                                                                                                                                         <  2:594562/151‑1 (MQ=255)
        aTGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATcc                                                                                                                                         <  1:1234318/151‑1 (MQ=255)
             ttctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGcc                                                                                                                                    <  2:904004/151‑1 (MQ=255)
             ttctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGcc                                                                                                                                    <  2:306227/151‑1 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:1759996/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:39788/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:419221/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:1602652/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:809938/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:1452030/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:916197/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:9496/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:1386821/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:970574/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  1:1252889/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  2:1688148/1‑148 (MQ=255)
               ctttATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGc                                                                                                                                     >  2:1721444/1‑148 (MQ=255)
                  tattaGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTgcag                                                                                                                               <  1:529925/151‑1 (MQ=255)
                               tttATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCCCTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACgg                                                                                                                  <  1:628514/151‑1 (MQ=255)
                                ttATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGATATCATCAATCCACGCCTGCAGCAGCGAAGAACGGt                                                                                                                 >  1:1006883/1‑151 (MQ=255)
                                     cGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTccc                                                                                                            <  2:566554/151‑1 (MQ=255)
                                       gCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc                                                                                                             >  1:1737739/1‑148 (MQ=255)
                                       gCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc                                                                                                             >  2:632068/1‑148 (MQ=255)
                                       gCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTcc                                                                                                             >  2:1774733/1‑148 (MQ=255)
                                       gCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCt                                                                                                           <  2:1416938/150‑1 (MQ=255)
                                           aaTTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtc                                                                                                      <  2:1552593/151‑1 (MQ=255)
                                            aTTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtca                                                                                                     >  2:82720/1‑151 (MQ=255)
                                            aTTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtca                                                                                                     >  1:1331620/1‑151 (MQ=255)
                                            aTTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtca                                                                                                     >  1:840522/1‑151 (MQ=255)
                                               ttGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatca                                                                                                  <  2:1730766/151‑1 (MQ=255)
                                               ttGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTtcatca                                                                                                  <  2:1741401/151‑1 (MQ=255)
                                                  aTTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                                                                                <  2:990032/150‑1 (MQ=255)
                                                  aTTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                                                                                <  1:1573974/150‑1 (MQ=255)
                                                  aTTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                                                                                <  2:168261/150‑1 (MQ=255)
                                                  aTTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAAc                                                                                                <  1:1423364/150‑1 (MQ=255)
                                                      ttACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAc                                                                                           <  2:7049/151‑1 (MQ=255)
                                                       tACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCAcc                                                                                          <  1:65824/151‑1 (MQ=255)
                                                         cTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCAt                                                                                        <  2:387940/151‑1 (MQ=255)
                                                           tgctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCa                                                                                      <  1:410103/151‑1 (MQ=255)
                                                             ctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCAc                                                                                     <  1:418919/150‑1 (MQ=255)
                                                             ctgcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCAc                                                                                     <  2:1302459/150‑1 (MQ=255)
                                                               gcCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActt                                                                                   >  2:480988/1‑150 (MQ=255)
                                                                cccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtct                                                                                 <  1:508068/151‑1 (MQ=255)
                                                                cccGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCActtc                                                                                  <  1:675358/150‑1 (MQ=255)
                                                                      aTTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAttt                                                                           <  2:1089937/151‑1 (MQ=255)
                                                                      aTTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAttt                                                                           <  1:802256/151‑1 (MQ=255)
                                                                           ccccTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGc                                                                      <  1:1247219/151‑1 (MQ=255)
                                                                                aTTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGt                                                                 >  1:79575/1‑151 (MQ=255)
                                                                                       ccACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAaataa                                                           <  1:886408/150‑1 (MQ=255)
                                                                                       ccACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAaataa                                                           <  1:363337/150‑1 (MQ=255)
                                                                                       ccACTGGGGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAaataa                                                           <  2:1196368/150‑1 (MQ=255)
                                                                                          cTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATgcg                                                       <  2:630526/151‑1 (MQ=255)
                                                                                                     aaCATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGa                                            >  1:13951/1‑151 (MQ=255)
                                                                                                     aaCATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGa                                            <  1:1483727/151‑1 (MQ=255)
                                                                                                      aCATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGAt                                           >  2:1285925/1‑151 (MQ=255)
                                                                                                         tGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc                                        <  1:1160444/151‑1 (MQ=255)
                                                                                                         tGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc                                        <  1:1372811/151‑1 (MQ=255)
                                                                                                           aaaGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACaa                                      <  1:661572/151‑1 (MQ=255)
                                                                                                            aaGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAAt                                     <  2:349912/151‑1 (MQ=255)
                                                                                                               gAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAAtt                                    <  1:1355565/149‑1 (MQ=255)
                                                                                                               gAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTc                                   <  1:631809/150‑1 (MQ=255)
                                                                                                                 aCCTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCgg                                <  1:529180/151‑1 (MQ=255)
                                                                                                                  ccTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCgg                                >  1:1712386/1‑150 (MQ=255)
                                                                                                                   cTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCGACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCa                              <  2:1016545/151‑1 (MQ=255)
                                                                                                                   cTGAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCa                              <  1:317992/151‑1 (MQ=255)
                                                                                                                     gAATTTTTTCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg                            >  2:781085/1‑151 (MQ=255)
                                                                                                                        ttttttCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGc                         <  1:1180181/151‑1 (MQ=255)
                                                                                                                             tCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaa                    <  1:564493/151‑1 (MQ=255)
                                                                                                                             tCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaa                    <  2:1041934/151‑1 (MQ=255)
                                                                                                                             tCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaa                    <  1:16650/151‑1 (MQ=255)
                                                                                                                             tCTGGTGGTAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaa                    <  1:484963/151‑1 (MQ=255)
                                                                                                                                  tggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAcc               <  2:1452687/151‑1 (MQ=255)
                                                                                                                                  tggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAcc               <  2:1168756/151‑1 (MQ=255)
                                                                                                                                  tggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc                <  1:556239/150‑1 (MQ=255)
                                                                                                                                   ggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCa              >  1:803110/1‑151 (MQ=255)
                                                                                                                                   ggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCa              >  1:1158279/1‑151 (MQ=255)
                                                                                                                                   ggtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCa              >  1:1523023/1‑151 (MQ=255)
                                                                                                                                    gtAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCAt             >  2:350339/1‑151 (MQ=255)
                                                                                                                                     tAATGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCAt             <  2:1313704/150‑1 (MQ=255)
                                                                                                                                      aaTGACGTATCGCTATCATCAATCCCCGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATAAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAg           <  2:730222/151‑1 (MQ=255)
                                                                                                                                      aaTGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAg           <  2:681522/151‑1 (MQ=255)
                                                                                                                                       aTGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGa          <  1:736481/151‑1 (MQ=255)
                                                                                                                                       aTGACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGa          <  1:1716274/151‑1 (MQ=255)
                                                                                                                                         gACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc                                        <  1:652609/119‑1 (MQ=255)
                                                                                                                                         gACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaa                    <  1:1420432/139‑1 (MQ=255)
                                                                                                                                         gACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaa                    >  2:1421078/1‑139 (MQ=255)
                                                                                                                                         gACGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTACCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc                                        >  2:652895/1‑119 (MQ=255)
                                                                                                                                          aCGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGaaaa       >  2:170063/1‑151 (MQ=255)
                                                                                                                                          aCGTATCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGaaa        <  2:1332213/150‑1 (MQ=255)
                                                                                                                                               tCGCTATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGAAAAAATGa  >  2:665837/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATAAAAAATGCTTTCTTTATTAGTGGGCAGTTTATTCGGCATAATTTTGATTTTTACTTGCTGCCCGGAATTTGCCCCTATTTTTGCCACTGGTGGCGTTAACATGAAAGGAACCTGAATTTTTTCTGGTGGTAATGACGTATCGCCATCATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGAAAAAATGA  >  NC_000913/2191285‑2191578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: