BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,700	T→C	L323P (CTT→CCT)	slt →	lytic murein transglycosylase, soluble

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,700	0	T	C	100.0%	378.3 / NA	103	L323P (CTT→CCT)	slt	lytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (56/47); total (56/47)

Only 100 of 103 total aligned reads displayed.

ATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACT  >  NC_000913/4631553‑4631847

aTGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                                                                                   >  1:718673/1‑151 (MQ=255)

 tGAAGATCAGATTCAGGAGCTGCGCGATTTCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGc                                                                                                                                                  <  2:311937/151‑1 (MQ=255)

  gAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCa                                                                                                                                                 >  2:722170/1‑151 (MQ=255)

   aaGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCAc                                                                                                                                                <  2:1309584/151‑1 (MQ=255)

    aGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCAc                                                                                                                                                >  1:773138/1‑150 (MQ=255)

     gATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCg                                                                                                                                              >  1:225107/1‑151 (MQ=255)

     gATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCg                                                                                                                                              >  1:1756441/1‑151 (MQ=255)

         aGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGa                                                                                                                                          >  2:536610/1‑151 (MQ=255)

         aGATCCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGa                                                                                                                                          >  2:1181658/1‑151 (MQ=255)

          gATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                                                                         >  2:234064/1‑151 (MQ=255)

          gATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                                                                         >  1:93371/1‑151 (MQ=255)

           aTTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                                                                         >  2:1360562/1‑150 (MQ=255)

            ttCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCg                                                                                                                                           >  2:676177/1‑147 (MQ=255)

            ttCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCg                                                                                                                                           <  1:675878/147‑1 (MQ=255)

            ttCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                                                                       >  1:992768/1‑151 (MQ=255)

            ttCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                                                                       >  2:1295560/1‑151 (MQ=255)

            ttCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                                                                       >  1:1016304/1‑151 (MQ=255)

             tCAGGGGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcgt                                                                                                                                      <  2:808148/151‑1 (MQ=255)

             tCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcgt                                                                                                                                      <  1:321284/151‑1 (MQ=255)

                 gAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTcgcg                                                                                                                                  >  2:1620518/1‑151 (MQ=255)

                   gCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCgg                                                                                                                                 >  2:1060232/1‑150 (MQ=255)

                     tGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCt                                                                                                                              <  1:923870/151‑1 (MQ=255)

                     tGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCt                                                                                                                              <  1:1152954/151‑1 (MQ=255)

                     tGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCt                                                                                                                              <  1:1087464/151‑1 (MQ=255)

                      gcgcGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGc                                                                                                                                >  1:369266/1‑148 (MQ=255)

                         cgATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAAt                                                                                                                          <  2:1450658/151‑1 (MQ=255)

                           atatCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAc                                                                                                                        <  1:494607/151‑1 (MQ=255)

                            tatCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAcc                                                                                                                       <  2:505006/151‑1 (MQ=255)

                             atCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATAc                                                                                                                        >  2:796933/1‑149 (MQ=255)

                             atCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACct                                                                                                                      <  2:667093/151‑1 (MQ=255)

                              tcgtcgCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                                                                                     <  2:256534/151‑1 (MQ=255)

                                       ggCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGcgcg                                                                                                            >  2:1071826/1‑151 (MQ=255)

                                       ggCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACC‑GCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGt                                                                                                           >  2:1184314/1‑151 (MQ=255)

                                                 gggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                                                                                  >  2:844169/1‑151 (MQ=255)

                                                 gggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                                                                                  >  2:1727205/1‑151 (MQ=255)

                                                 gggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCAGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                                                                                  >  2:414572/1‑151 (MQ=255)

                                                  ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg                                                                                                 >  1:139868/1‑151 (MQ=255)

                                                  ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                                                                                  <  2:222776/150‑1 (MQ=255)

                                                   gCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGAACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                                                                                >  2:759500/1‑151 (MQ=255)

                                                    cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                                                                                <  1:1027752/150‑1 (MQ=255)

                                                    cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                                                                                <  2:93403/150‑1 (MQ=255)

                                                    cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                                                                                <  2:1705286/150‑1 (MQ=255)

                                                    cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                                                                                <  1:175279/150‑1 (MQ=255)

                                                    cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                                                                                <  2:783860/150‑1 (MQ=255)

                                                    cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                                                                                <  2:466430/150‑1 (MQ=255)

                                                     aaCGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACGTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAg                                                                                              <  1:1722222/151‑1 (MQ=255)

                                                       cGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGCAGCg                                                                                            >  2:764894/1‑151 (MQ=255)

                                                         aTGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                                                                                          >  1:1724650/1‑151 (MQ=255)

                                                           gTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaag                                                                                        >  2:26993/1‑151 (MQ=255)

                                                             cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagag                                                                                      <  2:809530/151‑1 (MQ=255)

                                                                  acgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaaga                                                                                 <  1:1420712/151‑1 (MQ=255)

                                                                   cgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCGCCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGAt                                                                                <  2:1091800/151‑1 (MQ=255)

                                                                   cgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGAt                                                                                <  2:1150416/151‑1 (MQ=255)

                                                                         ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATgg                                                                          >  2:738176/1‑151 (MQ=255)

                                                                          gCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATgg                                                                          <  1:1438524/150‑1 (MQ=255)

                                                                          gCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATgg                                                                          <  1:976724/150‑1 (MQ=255)

                                                                          gCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTAGTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATgg                                                                          <  1:813633/150‑1 (MQ=255)

                                                                                   cgcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc                                                                <  2:63253/151‑1 (MQ=255)

                                                                                       aTGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAgg                                                             >  1:888177/1‑150 (MQ=255)

                                                                                             ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGG‑GGGttt                                                     >  2:968620/1‑151 (MQ=255)

                                                                                             ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGttt                                                     >  2:737604/1‑151 (MQ=255)

                                                                                             ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGttt                                                     >  2:1021055/1‑151 (MQ=255)

                                                                                               aTTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAACGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTAt                                                   >  1:1431172/1‑151 (MQ=255)

                                                                                               aTTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGG‑GGGttttt                                                   >  1:804238/1‑149 (MQ=255)

                                                                                                         cGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGG‑GGGTTTATTGCGGGaagg                                         >  2:1633771/1‑149 (MQ=255)

                                                                                                         cGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGG‑GGGGTTATTGCTGGGAcg                                         >  2:1126034/1‑151 (MQ=255)

                                                                                                           cAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGG‑GGGTTTATtggtgggaaggg                                       >  1:1507784/1‑145 (MQ=255)

                                                                                                             aTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGa                                     <  1:1327184/151‑1 (MQ=255)

                                                                                                               cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGCAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGGTTTTTGGTGGGAAGCggggg                                   >  1:377876/1‑148 (MQ=255)

                                                                                                               cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTTTTGCTGGGACGCGGGCg                                   >  2:1545241/1‑151 (MQ=255)

                                                                                                                     cGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTTTTTCTGGAACGCGGACGggaag                              >  2:651854/1‑150 (MQ=255)

                                                                                                                     cGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTATTGCTGGAACACGGACGtgaag                              >  2:836904/1‑150 (MQ=255)

                                                                                                                     cGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGtgaagc                             <  2:1258385/151‑1 (MQ=255)

                                                                                                                     cGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGtgaagc                             <  2:621347/151‑1 (MQ=255)

                                                                                                                       cTTATTGAACGCCGTGTCCGAATGGCGCCTGGCGCCGGCGATCGTCGCGGCCTGAATGCCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGtgaagct                            <  1:870989/150‑1 (MQ=255)

                                                                                                                        ttATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTTTTTCTGGGACGCGGAGGtgaagccta                          >  1:1730950/1‑148 (MQ=255)

                                                                                                                          aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTATTGCTGGGACGCGGGCGggaagctgaag                        >  1:848022/1‑151 (MQ=255)

                                                                                                                          aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTTTTGCTGGAACGCGGAAGtgaagctgaaa                        >  1:1273512/1‑150 (MQ=255)

                                                                                                                          aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTTGAACGCGGACGggaaactgaag                        >  2:898326/1‑151 (MQ=255)

                                                                                                                                cGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAag                  <  1:882302/151‑1 (MQ=255)

                                                                                                                                cGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAag                  <  2:109654/151‑1 (MQ=255)

                                                                                                                                cGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAag                  <  2:1230059/151‑1 (MQ=255)

                                                                                                                                cGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAag                  <  2:139913/151‑1 (MQ=255)

                                                                                                                                cGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAag                  <  1:252462/151‑1 (MQ=255)

                                                                                                                                  ccGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGG‑GGGTTTTTTGCTGGGACGGGGGCGTGGAACTGAAAGAAAaaag                >  1:438938/1‑151 (MQ=255)

                                                                                                                                  ccGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAagag                <  2:535529/151‑1 (MQ=255)

                                                                                                                                  ccGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAagag                <  1:1544512/151‑1 (MQ=255)

                                                                                                                                    gtgtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGGTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGAt              >  1:270903/1‑151 (MQ=255)

                                                                                                                                    gagtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGACAGAGAAAGGTGAATGGCGTTACTGGCAGGC‑CGGTTTATTGCTGGGACCCGGAGGCGCAGCTGATGGCAAAAAGAt              >  1:1221891/3‑151 (MQ=255)

                                                                                                                                     ggtACGAATGGCGCCTGGCACCGGCGATCGGCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGAt              <  2:1431822/149‑1 (MQ=255)

                                                                                                                                      gtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTTTTGCTGGGACGCGGACGTGAAGCTGAAGCAAAAAAGAttt            >  2:650996/1‑151 (MQ=255)

                                                                                                                                       tACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGAtttt           >  2:1568235/1‑151 (MQ=255)

                                                                                                                                       caCGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTATTTCTTGGACGCGGACGTGAAGCTGAAGCAAAAGAGAtttt           >  1:281592/2‑151 (MQ=255)

                                                                                                                                         cGAATGGCACCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGGTTTTATTTTTGGAA‑GCGGAGGTGGAGCTGAAGCAAAAGGGActtttt         >  1:583840/1‑149 (MQ=255)

                                                                                                                                               gcgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGCAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGGTTTATTGCTGGAAAGCGGACGTGAAGCTGTAGCAAAAGAGGTTTTGCATCaaa   >  1:1272578/1‑150 (MQ=255)

                                                                                                                                                cgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGGGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACt  <  2:259433/151‑1 (MQ=255)

                                                                                                                                                cgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACt  <  1:564872/151‑1 (MQ=255)

                                                                                                                                                cgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACt  <  2:1089491/151‑1 (MQ=255)

                                                                                                                                                cgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACt  <  2:897657/151‑1 (MQ=255)

                                                                                                                                                cgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACt  <  1:1252970/151‑1 (MQ=255)

ATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGC‑GGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACT  >  NC_000913/4631553‑4631847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`