Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 428,188 G→T R181L (CGC→CTC)  secD → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913428,1880GT99.1% 452.9 / NA 114R181L (CGC→CTC) secDSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (64/49);  total (64/50)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CTTCCTGATGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAG  >  NC_000913/428042‑428333
                                                                                                                                                  |                                                                                                                                                 
  tCCTGATGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATg                                                                                                                                              >  2:1368099/1‑150 (MQ=255)
   ccTGATGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATg                                                                                                                                              >  2:1754749/1‑149 (MQ=255)
       aTGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAg                                                                                                                                        <  2:1423060/151‑1 (MQ=255)
       aTGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAg                                                                                                                                        <  1:1655122/151‑1 (MQ=255)
       aTGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAg                                                                                                                                        >  1:163392/1‑151 (MQ=255)
       aTGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAg                                                                                                                                        <  2:1355814/151‑1 (MQ=255)
        tGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGc                                                                                                                                       <  1:1669321/151‑1 (MQ=255)
        tGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGc                                                                                                                                       <  2:1245855/151‑1 (MQ=255)
          gAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCt                                                                                                                                      >  1:628717/1‑150 (MQ=255)
              tGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga                                                                                                                                 <  2:581697/151‑1 (MQ=255)
              tGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga                                                                                                                                 <  2:798514/151‑1 (MQ=255)
               ggATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgat                                                                                                                                >  2:566042/1‑151 (MQ=255)
               ggATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgat                                                                                                                                >  2:1567027/1‑151 (MQ=255)
                gATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatg                                                                                                                               >  1:583198/1‑151 (MQ=255)
                gATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatg                                                                                                                               >  2:1458918/1‑151 (MQ=255)
                gATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatg                                                                                                                               >  2:1391868/1‑151 (MQ=255)
                gATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgat                                                                                                                                <  2:412934/150‑1 (MQ=255)
                gATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGCATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatg                                                                                                                               >  2:233999/1‑151 (MQ=255)
                   atGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAg                                                                                                                            <  2:582322/151‑1 (MQ=255)
                    tGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAg                                                                                                                            >  1:1193804/1‑150 (MQ=255)
                     ggACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGc                                                                                                                           >  1:903607/1‑150 (MQ=255)
                      gACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGcc                                                                                                                          <  2:1646627/150‑1 (MQ=255)
                          ccGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGc                                                                                                                     <  1:1346335/151‑1 (MQ=255)
                               cTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATc                                                                                                                <  2:457574/151‑1 (MQ=255)
                               cTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATc                                                                                                                <  2:965880/151‑1 (MQ=255)
                               cTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATc                                                                                                                <  1:1361021/151‑1 (MQ=255)
                                 tGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTAt                                                                                                                 >  2:1102767/1‑148 (MQ=255)
                                  ggCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGa                                                                                                             >  2:885043/1‑151 (MQ=255)
                                  ggCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGCATACCACTGTTCGTAAAGAAAACAACTACGTCCCGAGCATCACTTTCCTCGATGCCAAAGCTCGCGATGAAGCCATTGCGCATCTGa                                                                                                             >  1:1559495/1‑151 (MQ=255)
                                     aaaCTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCa                                                                                                          <  2:897641/151‑1 (MQ=255)
                                        cTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAg                                                                                                        >  1:862626/1‑150 (MQ=255)
                                         tCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcg                                                                                                      <  1:1060140/151‑1 (MQ=255)
                                             ggAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCAt                                                                                                  >  2:595437/1‑151 (MQ=255)
                                               aaCAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATcc                                                                                                <  1:1350226/151‑1 (MQ=255)
                                                   aaaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAc                                                                                            <  1:565834/151‑1 (MQ=255)
                                                    aaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATCCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAcc                                                                                           <  1:740600/151‑1 (MQ=255)
                                                    aaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAc                                                                                            >  2:1442424/1‑150 (MQ=255)
                                                       atCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTgg                                                                                        >  2:1461891/1‑151 (MQ=255)
                                                        tCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt                                                                                       >  1:883565/1‑151 (MQ=255)
                                                         cGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTg                                                                                      <  2:205164/151‑1 (MQ=255)
                                                             aGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGCGATTa                                                                                  >  1:1029916/1‑151 (MQ=255)
                                                              gCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                                                                                 >  2:741793/1‑151 (MQ=255)
                                                               ccTACGCAGTGACCTGCTCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTTGTGATTag                                                                                 <  1:1091546/150‑1 (MQ=255)
                                                               ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagc                                                                                <  2:536434/151‑1 (MQ=255)
                                                               ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagc                                                                                <  2:1097644/151‑1 (MQ=255)
                                                               ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                                                                                 >  2:1454522/1‑150 (MQ=255)
                                                               ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                                                                                 >  1:1559700/1‑150 (MQ=255)
                                                               ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                                                                                 <  1:633684/150‑1 (MQ=255)
                                                                 tACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag                                                                              <  2:1296902/151‑1 (MQ=255)
                                                                  aCGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag                                                                              >  1:344267/1‑150 (MQ=255)
                                                                  aCGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag                                                                              >  2:1432643/1‑150 (MQ=255)
                                                                  aCGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag                                                                              >  1:1350124/1‑150 (MQ=255)
                                                                   cGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGcc                                                                            <  2:303920/151‑1 (MQ=255)
                                                                   cGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGcc                                                                            <  2:64148/151‑1 (MQ=255)
                                                                    gCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCa                                                                           >  2:1614566/1‑151 (MQ=255)
                                                                     ctgcagcgggcgcGGAAAAGGCCTCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAg                                                                          >  1:1510843/10‑151 (MQ=255)
                                                                       gTGACCTGCGCGGAAAGGGCATCCCGTATACAACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAggg                                                                        >  2:978437/1‑151 (MQ=255)
                                                                                     aaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtg                                                           >  1:654969/1‑150 (MQ=255)
                                                                                             cccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTa                                                  >  1:1157146/1‑151 (MQ=255)
                                                                                              ccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTaa                                                 <  1:1323057/151‑1 (MQ=255)
                                                                                              ccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTaa                                                 <  1:583048/151‑1 (MQ=255)
                                                                                              ccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTaa                                                 <  1:954606/151‑1 (MQ=255)
                                                                                              ccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGGaa                                                 >  2:366811/1‑151 (MQ=255)
                                                                                               cGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGGGCGGTAAt                                                >  2:586999/1‑151 (MQ=255)
                                                                                               cGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCACCTGCGGGCGGCAAt                                                >  2:157493/1‑151 (MQ=255)
                                                                                                gTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATg                                               >  1:1464370/1‑151 (MQ=255)
                                                                                                gTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATg                                               >  1:41068/1‑151 (MQ=255)
                                                                                                gTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATg                                               >  1:519241/1‑151 (MQ=255)
                                                                                                gTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATg                                               >  2:1042471/1‑151 (MQ=255)
                                                                                                gTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATg                                               >  2:1254149/1‑151 (MQ=255)
                                                                                                gTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATg                                               >  2:119368/1‑151 (MQ=255)
                                                                                                   taCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGc                                            <  1:1036540/151‑1 (MQ=255)
                                                                                                   taCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGc                                            <  2:709469/151‑1 (MQ=255)
                                                                                                     ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGa                                          >  1:96640/1‑151 (MQ=255)
                                                                                                     ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGa                                          >  1:199278/1‑151 (MQ=255)
                                                                                                     ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGa                                          <  1:387117/151‑1 (MQ=255)
                                                                                                        cTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCCAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATg                                        >  1:440287/1‑150 (MQ=255)
                                                                                                        cTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGc                                       <  1:371409/151‑1 (MQ=255)
                                                                                                         tGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCt                                      <  2:713153/151‑1 (MQ=255)
                                                                                                          gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCt                                      >  1:804268/1‑150 (MQ=255)
                                                                                                                   gAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTATAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTg                            >  2:482806/1‑151 (MQ=255)
                                                                                                                   gAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTg                            >  1:1355571/1‑151 (MQ=255)
                                                                                                                      aacaacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAg                         <  2:351449/151‑1 (MQ=255)
                                                                                                                          acTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGcgcg                     >  2:986621/1‑151 (MQ=255)
                                                                                                                          acTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGcgcg                     >  1:154288/1‑151 (MQ=255)
                                                                                                                          acTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGcgcg                     >  1:851200/1‑151 (MQ=255)
                                                                                                                                gCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAata               >  2:969856/1‑151 (MQ=255)
                                                                                                                                 ccTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAata               <  1:1367482/150‑1 (MQ=255)
                                                                                                                                    gAGCATCACTTTCCTCGATGCTAAGGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCg           >  1:538432/1‑151 (MQ=255)
                                                                                                                                    gAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCg           >  2:1579358/1‑151 (MQ=255)
                                                                                                                                    gAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCg           >  1:1215273/1‑151 (MQ=255)
                                                                                                                                      gCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGt         >  2:592500/1‑151 (MQ=255)
                                                                                                                                      gCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGGGAAGCGAGTGAATATGCggg         >  2:1576181/1‑150 (MQ=255)
                                                                                                                                      gCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCgg          <  1:918246/150‑1 (MQ=255)
                                                                                                                                       cATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCgg          <  1:1753886/149‑1 (MQ=255)
                                                                                                                                       cATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCgg          <  2:1490722/149‑1 (MQ=255)
                                                                                                                                           aCTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcagc    <  1:64912/151‑1 (MQ=255)
                                                                                                                                           aCTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgcagc    <  1:1266461/151‑1 (MQ=255)
                                                                                                                                             tttCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGcagcag  <  1:324784/151‑1 (MQ=255)
                                                                                                                                             tttCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGcagcag  <  1:816831/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTCCTGATGGAAGTGGATATGGACACCGCGCTTGGCAAACTCCAGGAACAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAG  >  NC_000913/428042‑428333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: