Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA56.9% 55.0 / 190.4 144A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (42/20);  new base A (0/82);  total (42/102)
Fisher's exact test for biased strand distribution p-value = 2.24e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-13

Only 100 of 144 total aligned reads displayed.

GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAT  >  NC_000913/3665553‑3665851
                                                                                                                                                             |                                                                                                                                                      
gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                                       >  1:997753/1‑151 (MQ=255)
gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                                       >  1:900022/1‑151 (MQ=255)
gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                                       >  1:433220/1‑151 (MQ=255)
      aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAcc                                                                                                                                                 <  2:1022175/151‑1 (MQ=255)
        cGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAg                                                                                                                                              >  1:1020638/1‑151 (MQ=255)
           gTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGt                                                                                                                                            >  2:904239/1‑150 (MQ=255)
           gTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGt                                                                                                                                            >  1:438405/1‑150 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  1:1030432/1‑151 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  2:1612264/1‑151 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  2:1264286/1‑151 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  1:1299237/1‑151 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                               >  1:436717/1‑151 (MQ=255)
                                gtgCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGata                                                                                                                      >  1:1667521/1‑151 (MQ=255)
                                     tGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGTCATTAAAATAGCGATATTCAc                                                                                                                 <  1:1163962/151‑1 (MQ=255)
                                      aaaGATTTAATCCCCCCCCCATGAGCAGCGGAAAAAAAAATGAAACCGTTCCCTTTGCCCAGAAAACAAAATTTACACCCCCCTTATCAACTGAAATTGGCTTGGCCCCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCAcc                                                                                                                <  1:60806/150‑1 (MQ=255)
                                         gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                             >  2:1206951/1‑151 (MQ=255)
                                          aTTTAATCCCTCCTCCTGAACCTTGGGGAAAAATAATAAAAGCGTTCCCTTGGACAGAAAAAAAAAATTTAAACCCCCTTAACCTCACCAATTTGGCTTTGCACCCCAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                              <  1:1013247/149‑1 (MQ=255)
                                           tttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTaa                                                                                                           >  1:495265/1‑151 (MQ=255)
                                            ttatcccctccccctTGAGCAGCGGAAAAAATAATAAAAGCGTGCCCCTTGACACAAATACAAAATTTTCACCACCCTTATCTCCTCAATTTTGTCTTGCACCCCCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                          <  1:324130/145‑1 (MQ=255)
                                             tatccccccctcctTGAGCACGGGGAAAAATAAAAAAAGGGTTCCCTTTGACACAAAAAAAAATTTTCCAACCCCTTTATCTCCCCAATTTTGGCTTCCATCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:376732/143‑1 (MQ=255)
                                             tacccccccctcctAGGGTATCGGGAAAAATAATGAATGCGTGCCCCGAAACACGAAAAAAAATTTTTCAACACTCTTATCTACTCAAATTTGGCTAACACCCGAAAAACCCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:1581685/143‑1 (MQ=255)
                                             tAATGCCTCCTCCTTGTGCAGGGGAAAAAAAAAGAAAGGCGTGCCCCTGGACAAAAAAACAAAATTTCCACCCCACTTATCTACTAAATTTGGCCTTGCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  1:1364978/151‑1 (MQ=255)
                                             tAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGCCACAAAAAAAAAATTTTCAACACACTTATCTCCTCGAATTTGGCTTGCACCCGAAAAAACCAGGACACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:1362252/151‑1 (MQ=255)
                                             tAATCCCCCCTCCTGGAAAATGGGGAAAAATAATAAAGGCGTGTCCCTTGAAACAAAAAAAAAATTTTCAACACACTTTTCTCCTCAATTTTGGCTTGCACCCGAAAAACCCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                         <  2:830535/151‑1 (MQ=255)
                                                 gCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                     >  2:721796/1‑151 (MQ=255)
                                                 ccccccccctTAAGCTTGGGGAAAAATAATGAAAGGGTGTCCTTTGACACAAATAAAAATTTTACACCACCTTTTCCTCCTCAAATTGGGTTTACACCCGAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                     <  1:571245/144‑1 (MQ=255)
                                                   ctccccctTGAGCTTGGGAAAAAATAATAAAACCGTTTCCCTTGAAACAAATACAAATTTTCCAACCCCCTTATCTCCTCAATTTTGGCTTGCACCCACAAAAACCAGTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                   <  2:1634422/151‑1 (MQ=255)
                                                   ccccccctTGAGCATGGGGAAAAAAAAGAAAAGCGTGCCCCTTGACACAAATAAAAAATTTCCAACCCCCTTATCTACTCAATTTTGGCTTGCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                   <  2:711318/146‑1 (MQ=255)
                                                    tcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                  >  1:996199/1‑151 (MQ=255)
                                                     cctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTCGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag                                                                                                 >  1:1678210/1‑151 (MQ=255)
                                                       tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                               >  2:1529700/1‑151 (MQ=255)
                                                       ccctTGAGCATGGGGAAAAAAAAAAAAAGCGTGTCCTTTGACACGAAAAAAAAATTTTCAACCCACTTATCTCCTCAATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                               <  2:1517416/150‑1 (MQ=255)
                                                        cctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                              >  2:909032/1‑151 (MQ=255)
                                                             accATCGGAAAAAATAATGAAAGCGTGTCCTTTACCACAAAAAAAAAATTTTCAACACCCTTATCTCCCCGAATTTGGCTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:1207647/149‑1 (MQ=255)
                                                             acaatgggaaaaaaaaaTGAAAGCGTGTCCCTTAACAAAAAAAAAAAATTTTAACCACACTTATCAACTCAATTTTGGTTTCCACCCAAAAAAACCGGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:1244607/140‑1 (MQ=255)
                                                               cagggggAAAAATAATAAAAGCGTGTCCCTTGACACAAATACAAAATTTTAAACACACTTATCTACTAAATTTTGTTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:1656164/145‑1 (MQ=255)
                                                               cATGGGGAAAAATAATAAAGGCTTGTCCTTTGACACAAATACAAAATTTTCAACCCACTTATCTACTCGATTTTGGCTTGCACCCAAAAAAACCAGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:490727/149‑1 (MQ=255)
                                                               cATCGGGAAAAAAAATGAAAGCGTGTCCCTTGACAGAAATAAAAAATTTTCAACCCCCTTATCTACCCGAATTTGGCTTGCACCCGCAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:884937/149‑1 (MQ=255)
                                                               cATCGGAAAAAAAAATGAAAGCGTGTCCCTTGACACAAATACAAAATTTCCAACACACTTATCTACTCGAATTTGGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  2:146325/149‑1 (MQ=255)
                                                               aatggggAAAAAAAAAAAAAGCGTTTCCCTACACAAAAACAAAAAATTTAAAACACACTTATCTTCTAAAATTGGACTAACACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                                       <  1:1617574/147‑1 (MQ=255)
                                                               aaTCGGAAAAAATAATGAAGGCGTGTCCCTTGACACAAATAAAAAATTTCCAACACATTTATCTACTCAATTTTGGTTTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                         <  1:459030/148‑1 (MQ=255)
                                                                 tggggAAAAAAAATGAAGGCGTGTCCCTTGACACAAATAAAAAATTTTCAACCCCTTTACCTCATCAATTTTGTCTTCCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                     <  1:153069/149‑1 (MQ=255)
                                                                 tCGGGAAAAATAATGAAAGCGTGCCCCTTGACACGAATAAAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                                     <  2:749833/151‑1 (MQ=255)
                                                                  cggaaaaaaTAATAAAGCCGTGTCCTTTAACACGAAAACAAAATTATCAACCCACTTACCTCCTAAATTTTGCTTTCCCCCCAAAAAAACAAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  1:1328611/145‑1 (MQ=255)
                                                                  cGGGAAAAATAAAGAAAGCGTTCCCCTTGCCACAAAAAAAAATTTTTCAACACACTTATCTACTCAATTTTGGCTTCCCCCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                                       <  2:1741846/148‑1 (MQ=255)
                                                                   ggtaaaaaaaaTAAAAGCTTGCCCCTTGAAACGAATACAAAATTTCCACCACTTTTTTTTGCTGAAATTGGTTTTGCCTCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  2:1526795/145‑1 (MQ=255)
                                                                   gggAAAAATAATAAAGGCTTATCCCTTAACAAGAATAAAAAATTTTCAACCCCCTTTTCTAGTCAATTTTGGTTTCCTCCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  1:1051011/150‑1 (MQ=255)
                                                                   gggAAAAAAAATAAAGGCTTTCCCCTTGACACAAAAACAAATTTTTAAACACCTTTCTCTTATCAAATTTGGTTTCCTCCCGAAAAACCGTGTTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                    <  2:769765/150‑1 (MQ=255)
                                                                     aaaaaaaaaTAAAAGCGTGTCCCTTGACACAAAAAAAAAATTTTCAACCCACTTATCTACTAAATTTTGCTTTGCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCt                                                                                  <  1:95988/147‑1 (MQ=255)
                                                                        aaaTAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                              >  2:1531980/1‑151 (MQ=255)
                                                                        aaaTAATGAAACGGTTTCCCTGCTCACAAATACAAAATTTTCAACCCCCTTTTCTACCGAATTTGGGCTCGCACCCGCAAAACCCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                              <  2:1677450/151‑1 (MQ=255)
                                                                        aaaTAATAAAAGCGTGCCCCTAAACACGAATAAAAATTTTTCACCACTTTTTTCTCCTGAATTTTGTCTTCCCCCCGCAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGcn                                                                              <  2:456868/151‑2 (MQ=255)
                                                                           taatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                            >  1:1164469/1‑150 (MQ=255)
                                                                            aatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                          >  2:1624224/1‑151 (MQ=255)
                                                                                    gCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCCCAAAAACCCGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTaa                                                                   >  2:260249/1‑150 (MQ=255)
                                                                                       tttCCCTTGACCCAAAAAAAAAATTTCCACCCCCTTTATCTCCAGGATTTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  2:970002/148‑1 (MQ=255)
                                                                                       ttccccTTGACACAAAAAAAAAATTTACAACCCACTTATCTACTCAAATTTGGTTTGCACCCACAAAAACAGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  1:766801/147‑1 (MQ=255)
                                                                                       tgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACa                                                                >  2:335047/1‑151 (MQ=255)
                                                                                       tgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACa                                                                >  2:703975/1‑151 (MQ=255)
                                                                                       tgccccTTAACACAAATAAAAAATTTTCAACACCTTTATCTACTCAATTTTGGCTTGCCTCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                 <  2:177252/147‑1 (MQ=255)
                                                                                         tCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                              >  1:748523/1‑151 (MQ=255)
                                                                                                 acacAAAAAAAAAATTTTCAACACCTTTATCTACCCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                                       <  2:416091/151‑1 (MQ=255)
                                                                                                    caaaaaaaaaaTTTACAACACCCTTATCTCCCCAATTTTGGCTTCCACCCAAAAAACCCAGGCCCCCGCCATTAAAATAGCGATATCCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  2:968318/144‑1 (MQ=255)
                                                                                                    caaaTAAAAAATTTTCAACCCCCTTATCTCCTCAATTTTGGCTTGCATCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  2:1589422/149‑1 (MQ=255)
                                                                                                    caaaTAAAAAATTTCCAACACCCTTATCTACTAAATTTGGGCTTACATCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATACTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  1:1430696/149‑1 (MQ=255)
                                                                                                    cGAATACAAAATTTTCACCACACTTATCTCCTCGAATTTGCTTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  1:1039905/151‑1 (MQ=255)
                                                                                                    aaaaaaaaaaaTTTTCAACACACTTATATCCTAGAATTTGGCTTGCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  1:1228905/144‑1 (MQ=255)
                                                                                                    aaaaaaaaaaaTTTAAAACCCCCTTATCTCCCAAATTTTGGTTTGACCCGAAAAAACCGGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  1:1541442/144‑1 (MQ=255)
                                                                                                    aaaaTAAAAAATTTTCAACCCCTTTTTCTTCCCGATTTTGGCTTACCCCAGAAAAAACCGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  2:1344269/149‑1 (MQ=255)
                                                                                                     aaaaaaaaaaTTTTACAACCCATTTACCCCCTCGATTTTGGCTTGAACCCGAAAAAACCGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  2:518222/145‑1 (MQ=255)
                                                                                                     aaaaaaaaaaTTTTCAAACCCCTTTATCTCCTCAATTTTGGTTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  1:880534/145‑1 (MQ=255)
                                                                                                     aaaTACAAAATTACCAACTCAGTTATCTACCAAATTTGGGCTGGCACCCAAAAAAACCGGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                    <  1:1401642/149‑1 (MQ=255)
                                                                                                             aaTTTTAAACCCCCTTATCTCCTCGATTTTGGCTTGCACCCAAAAAAACAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                           <  2:131563/151‑1 (MQ=255)
                                                                                                              aTTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATa                                          >  2:635776/1‑151 (MQ=255)
                                                                                                                 ttacAACACCTTTATCTCCTAGATTTTGGTTTATCCCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                        <  2:1376338/148‑1 (MQ=255)
                                                                                                                  ttcaaaCACCTTTATCTACTCGATTTTGGCTTCCATCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                        <  1:1464387/147‑1 (MQ=255)
                                                                                                                         ccacTTATCACCTCAATTTTGGTTTCCACCCAAAAAAACGAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                                 <  1:533192/150‑1 (MQ=255)
                                                                                                                          cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  2:797692/1‑151 (MQ=255)
                                                                                                                          cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  1:974338/1‑151 (MQ=255)
                                                                                                                          cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                                >  1:1701568/1‑151 (MQ=255)
                                                                                                                            tttATCTACTCAATTTTCGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                              <  1:1373624/150‑1 (MQ=255)
                                                                                                                            cTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                              >  2:1278758/1‑151 (MQ=255)
                                                                                                                            cTTAACTACTAGAATTTGGCTTGCATCCGCAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTACGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGGAGTGATTGCATAGTTGAATTAatt                              >  1:1574811/1‑150 (MQ=255)
                                                                                                                             ttATCTCCTCAATTTGGGTTTCCATCCCAAAAAACAAGCTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  2:47500/151‑1 (MQ=255)
                                                                                                                             ttATCTACTCGATTTTGCCTTGCACCCACAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  1:1623416/151‑1 (MQ=255)
                                                                                                                             ttATCTACTCAAATTTGGTTTCCATCCACAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  1:1455728/151‑1 (MQ=255)
                                                                                                                             ttATCTACAAAATTTTGGCTTCCACCCGCAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                             <  1:321185/151‑1 (MQ=255)
                                                                                                                              tATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                            >  2:1480742/1‑151 (MQ=255)
                                                                                                                                  tcctaaattttGGCTTGCTCCCGCAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                        <  2:838732/143‑1 (MQ=255)
                                                                                                                                  tactaaaaTTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                        <  1:1206360/145‑1 (MQ=255)
                                                                                                                                  tACTCGAATTTGGCTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                        <  2:1441888/151‑1 (MQ=255)
                                                                                                                                      aaaaTTTGGCTTGCACCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                    <  1:703611/149‑1 (MQ=255)
                                                                                                                                        attttGCCTTCCACCCGAAAAACCCGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                  <  2:847414/149‑1 (MQ=255)
                                                                                                                                        aaTTTTGCTTTGCATCCAAAAAAACCAGGCAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACa                   <  1:1451158/151‑1 (MQ=255)
                                                                                                                                        aaTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                  >  1:1554890/1‑151 (MQ=255)
                                                                                                                                        aaTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                  >  2:1051194/1‑151 (MQ=255)
                                                                                                                                               gCTTGCCTCCGAAAAAACCGGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  2:1523661/150‑1 (MQ=255)
                                                                                                                                               gCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  2:823062/150‑1 (MQ=255)
                                                                                                                                                 ctttGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc             <  1:178971/148‑1 (MQ=255)
                                                                                                                                                   ttGCACCCAAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGt          <  2:1030979/151‑1 (MQ=255)
                                                                                                                                                             |                                                                                                                                                      
GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAT  >  NC_000913/3665553‑3665851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: