Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA60.8% 33.7 / 104.2 97intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (6/32);  new base A (59/0);  total (65/32)
Fisher's exact test for biased strand distribution p-value = 6.23e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-12

ATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACC  >  NC_000913/2386722‑2387007
                                                                                                                                             |                                                                                                                                                           
aTCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑c‑ac                                                                                                                                                 <  1:822879/151‑1 (MQ=255)
   gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TTCAGAAAAAGAGACATA‑T‑ACa‑tt                                                                                                                                             >  1:295700/1‑151 (MQ=255)
   gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAACGACATG‑C‑ACa‑tt                                                                                                                                             >  2:1234577/1‑151 (MQ=255)
   gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCCGAAAAAAAGACAAG‑g‑acaat                                                                                                                                              >  2:262804/1‑149 (MQ=255)
        aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TTCAGAAAAAACGACAAG‑G‑ACa‑atttttt                                                                                                                                        >  1:79151/1‑151 (MQ=255)
        aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCACAAAAAAAGACaaggaaaatttttt                                                                                                                                           >  1:1433770/1‑138 (MQ=255)
        aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAACGAAATG‑C‑ACa‑ttttttt                                                                                                                                        >  2:1328045/1‑151 (MQ=255)
        aaaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGCTATAACTTTGTTATACAAAAGTAAATAGTAAAAATTTCTCGAGGCGGG‑GCCCGAAAAAAAGGAAAG‑C‑ACa‑ttttttt                                                                                                                                        >  2:1627731/1‑151 (MQ=255)
           cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TTCCGAAAAAAAGAAAAA‑C‑ACa‑tttttttttt                                                                                                                                     >  2:654753/1‑150 (MQ=255)
           cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAAAGACAAG‑A‑AAa‑tttttttttt                                                                                                                                     >  1:860560/1‑150 (MQ=255)
           cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCAGAAAAAAAGACAAG‑G‑AAa‑tttttttttt                                                                                                                                     >  2:1135170/1‑150 (MQ=255)
              tACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCt                                                                                                                                  <  2:64071/151‑1 (MQ=255)
               aCCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCAAAAAAAACACAAG‑G‑AAA‑TTTTTTTTTTCCt                                                                                                                                  >  2:776471/1‑150 (MQ=255)
                  aCTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATc                                                                                                                              <  2:295499/151‑1 (MQ=255)
                   cTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGGAAAAAAGACAAG‑G‑ACA‑ATTTTTTTTTCCTcct                                                                                                                               >  1:894630/1‑147 (MQ=255)
                       cccGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCCGAAAAAACAAAATA‑C‑ACA‑ATTTTTTTTTCCCCCTC‑TG‑ttt                                                                                                                       >  1:1469799/1‑151 (MQ=255)
                           gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGT‑TCCAGAAAAAAAGAAAAG‑C‑AAA‑ATTTTTTTTTCCCCCTC‑TG‑tttttta                                                                                                                   >  2:264585/1‑148 (MQ=255)
                           gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAACGACAAG‑CAACT‑TTTTTTTTTCCCTC‑TC‑TT‑TTTTTAg                                                                                                                   >  2:1084761/1‑151 (MQ=255)
                           gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAAAGAAAAG‑A‑AAA‑TTTTTTTTTTCCTCCTC‑CT‑TTTTTAg                                                                                                                   >  1:1265130/1‑151 (MQ=255)
                            tGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGa                                                                                                                  <  2:1191152/151‑1 (MQ=255)
                                          gaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTccc                                                                                                                                   <  2:289493/122‑1 (MQ=255)
                                             tGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTc                                                                                              <  2:1170839/151‑1 (MQ=255)
                                               aaTTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCACAAAAAGAGACATG‑C‑ACA‑TTTTTTTTTTCTTCCTC‑TG‑TTTTTAGATG‑TGaacaaagtaactcaa                                                                                              >  1:1718461/1‑138 (MQ=255)
                                                aTTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AAc                                                                                          <  2:713613/151‑1 (MQ=255)
                                                   aTCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTGGAGGCGGG‑TCCAGAAAAAAAGAAAAA‑A‑CAA‑TTTTTTTTTTTCTCTTA‑TG‑TTTTTATATG‑TGGAACATG‑AAA‑TTT‑aaaagg                                                                                       >  1:1186573/1‑147 (MQ=255)
                                                   aTCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TGCAGAAAAAAAGACAAA‑C‑AAA‑CTTTTTTTTTTCTTTTC‑TT‑TTTTTAAATG‑CGAAAAAAC‑AAA‑ttttaacgg                                                                                        >  2:1643454/1‑144 (MQ=255)
                                                       gAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TTTAGAAAAAAAGACATA‑C‑ACA‑TTTTTTTTTTTCTCATT‑TG‑TTTTTAAATT‑TGAAAAAAG‑aaaatttaaaggggga                                                                                     >  1:14893/1‑138 (MQ=255)
                                                            tGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAaaata                                                                              <  2:748375/151‑1 (MQ=255)
                                                             ggCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAACGACAAA‑G‑ACA‑ATTTTTTTTTCCTCCTT‑TT‑TTTTTAGATG‑CGAAAAATG‑AAA‑ATT‑TAAGGAGGGAaaaaaa                                                                             >  1:1425433/1‑151 (MQ=255)
                                                              gCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TGCCGAAAAAAAGACAAT‑C‑ACA‑TTTTTTTTTTTCTCCTC‑TG‑TTTTTAAAAG‑CGAAAACAG‑AAA‑ATC‑AACCGGGGAAaaataaa                                                                            >  1:667742/1‑151 (MQ=255)
                                                                tCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATg                                                                          <  2:735753/151‑1 (MQ=255)
                                                                  aGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GGCCGGAAAAAAGAGATG‑C‑ACA‑TTTTATTTTTTCTCCTC‑TG‑TTTTTAAATG‑CGGAAAATGAAAA‑TTT‑AAGGGGGGAAACAAAAATgg                                                                         >  1:1272075/1‑151 (MQ=255)
                                                                   ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TTCCAAAAAAAAGACATA‑C‑ACACTTTTTTTTTTCCTCCTT‑TT‑TTTTTA‑AAG‑CGTAACATT‑AAA‑ATC‑CACGGAGGGAAAAAAAATtggg                                                                       >  1:1716724/1‑150 (MQ=255)
                                                                   ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCAGAAAAAACGACATG‑C‑ACA‑TTTTTTTTTTTCTCCTC‑CG‑TTTTTAGATG‑CGGAAAATA‑AAA‑ATT‑AACAGAGGAAAAAAAAATaggg                                                                       >  2:531434/1‑150 (MQ=255)
                                                                   ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GGCCAAAAAAACGACAAG‑C‑CCA‑CTTTTTTTTTCCTCATT‑TT‑TTTTAAAAAG‑GGAAAAAAGAAAA‑TTC‑AAAGGGGGAAAAAAAAATggg                                                                        >  2:591918/1‑151 (MQ=255)
                                                                    gTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCAGAAAAAAAAAAAAA‑C‑ACA‑TTTTATTTTTCCTTTTCTTG‑TTTTAAAAGG‑GGAAAAAGA‑AAA‑TTA‑AAGGGGGGAAAAAAAAATGGGa                                                                       >  2:551691/1‑151 (MQ=255)
                                                                    gTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCCGGAAAAACGACAAG‑G‑CCA‑TTTTTTTTTTCCTCCTCTTT‑TTTTAAAAGG‑GGAAAAAGA‑AAA‑TTT‑ACCGGGGGAAAAAAAAATGGGa                                                                       >  2:1023907/1‑151 (MQ=255)
                                                                    gTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCCGAAAAAAAGGCCCGCC‑AAA‑TTTTTTTTTCCTCCTCC‑TT‑TTTTAAATAG‑GGAAAAAGA‑AAA‑TTA‑AACGGGGGAAAAAAAAATGGGa                                                                       >  2:20213/1‑151 (MQ=255)
                                                                     tAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGGAAAAAAC‑ACAAG‑A‑ACA‑ATTTTTTTTTCCTCATCTTG‑TTTTTAATTG‑GGAAAAAGA‑AAA‑TTA‑AACGGGGGAAAAAAAAATGGGaa                                                                      >  1:336139/1‑151 (MQ=255)
                                                                        tGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGAt                                                                 <  1:827238/151‑1 (MQ=255)
                                                                        tGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGAt                                                                 <  1:153822/151‑1 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TTCCGAAAAAAAGAAATG‑C‑AAA‑TTTTTTTTTTCCCCATC‑TGTTTTTAAAAGG‑GAAAAAATA‑AAA‑TTA‑ACCGGGGGAAAAA‑AAATTGGAA‑TGATTgggtt                                                           >  1:216953/1‑148 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TTCAGAAAAAAACACAAG‑A‑ACA‑TTTTTTTTTTCCTCCTC‑TG‑TTTTTAGATT‑CGGAACAAG‑AAA‑TTT‑AAAGGAGGAAAAATAAAATGGGA‑TGATTTGCtt                                                           >  1:1580206/1‑150 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TTCAGAAAAAAAAACAAG‑C‑ACA‑TTTTATTTTTTCTCCTC‑TG‑TTTTTAGAAG‑AGAAACAAT‑AAA‑ATC‑TACAGAGGGAAAATAAAAGGGGA‑TGaattggta                                                           >  1:209209/1‑144 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TGCAGAAAAAAAGACAAG‑C‑AAA‑ATTTTTTTTTCCTCATC‑CGTTTTTAAAAGG‑GGAAAAAAA‑AAA‑TTA‑AAC‑GAGGAAAAAAAAATAGGGA‑TGAATGGCTa                                                           >  2:789956/1‑151 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCTAAAAAACGACATG‑A‑ACA‑TTTTTTTTTTCCTCCTC‑TG‑TTTTAAAAAG‑CGAAAAATG‑AAA‑ATC‑AAAGGAGGGAAAATAAAATGGAA‑AGATTGGGTa                                                           >  1:192004/1‑151 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAACGACATG‑C‑ACA‑TTTTATTTTTTCTCCTC‑TG‑TTTTTAGAAG‑CGGAAAATG‑AAA‑ATC‑AAAGGAGGAAAAAAAAAAGGGAA‑TTATTTGCTa                                                           >  1:813306/1‑151 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAACCACACG‑C‑ACC‑TTTTTTTTTTCCCCCTT‑TG‑TTTTTAGATT‑CGGAAAATC‑AAA‑TTC‑TACGGGGGGAAAAAAAATAGGGA‑TTATTTGCtt                                                           >  2:1187576/1‑150 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAAAGACATG‑C‑ACA‑TTTTTTTTTTCCTCCTC‑TG‑TTTTTAAAAG‑AGAAACAAG‑AAA‑TTT‑TAAGGGGGAAAAATAAATTGGGA‑AGATTGGGTa                                                           >  1:729713/1‑151 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCt                                                            <  1:1515701/150‑1 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAACGGCAAG‑G‑ACA‑TTTTTTTTTTCCTCATC‑TT‑TTTTTAGAAG‑CGGAACAAA‑AAA‑TTT‑AACGGGGGGAAAAAAAATGGGGA‑TGATTTGCtt                                                           >  2:418670/1‑150 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAACGAAAAG‑C‑AAA‑TTTTTTTTTTCCTCCTC‑TG‑TTTTAAGATG‑CGAAAAAAG‑AAA‑TTT‑AAAGGAGGAAAAAAAAATTGGAA‑TGTTTGGCtt                                                           >  2:649302/1‑150 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAAAAAAAACCAAATA‑T‑TCA‑TTTTATTTTTTCTCCTC‑TG‑GTTTTAGATA‑AGAAACATG‑AAA‑ATT‑TACTGAGGAAAAAAAAAAGGGGA‑AGATTTGCTa                                                           >  1:1734079/1‑151 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCAGAAAAAAAGACAAG‑A‑ACA‑TTTTTTTTTTCCTCTTC‑TG‑TTTTAAGATG‑GGAAACATG‑AAA‑ATT‑AACCGGGGAAAAATAAATTGGGA‑TGAtttggtt                                                           >  1:486867/1‑146 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCAGAAAAAAACAAATG‑C‑ACA‑TTTTATTTTTTCTCATC‑TG‑TTTTAAGATG‑GGGAACATG‑AAA‑ATC‑AAAGGCGGAAAAAAAAATAGGGA‑TGATTGGcga                                                           >  1:127670/1‑149 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCCAAAAAAACCACAAA‑G‑ACA‑TTTTTTTTTTCCTCCTC‑TG‑TTTTAAAATG‑CGAAAAAAG‑AAA‑ATCAAACGGGGGAAAAAAAATTGGGAT‑GAATTGGc                                                             >  2:1317772/1‑150 (MQ=255)
                                                                              gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAGACATG‑C‑ACA‑ATTTTTTTTTTCTCATC‑TG‑TTTTTAGATG‑CGAAACATG‑AAA‑ATC‑AAAGGAGGGAAAATAAATGGGGA‑TGATTGGCTa                                                           >  1:1429311/1‑151 (MQ=255)
                                                                               tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTAt                                                          <  1:583608/151‑1 (MQ=255)
                                                                               tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTAt                                                          <  1:1030933/151‑1 (MQ=255)
                                                                                gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAAAGAAATG‑C‑ACA‑TTTTTTTTTTCCTCATC‑TG‑TTTTTAAAAG‑CGAAACAAT‑AAA‑ATT‑AAAGGGGGAAAAAAAAAAGGGGA‑TGATTGGGTAtt                                                         >  2:156130/1‑151 (MQ=255)
                                                                                gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGaaaaa                                                                                <  1:567956/129‑1 (MQ=255)
                                                                                gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAAAGACAAA‑A‑ACA‑TTTTTTTTTTCCTCATC‑TG‑TTTTTATAAA‑CGAAAAAAT‑AAA‑ATT‑AACCGAGGGAAAATAAATTGGGA‑TGATTGGGTaat                                                         >  1:367498/1‑149 (MQ=255)
                                                                                gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAACGAAAAG‑C‑AAA‑TTTTTTTTTTCCTCATC‑TT‑TTTTTAAATGAGGAAAAATA‑AAA‑TTA‑AAGGGGGGAAAAAAAATTGGGattatttgttttt                                                           >  2:1680940/1‑139 (MQ=255)
                                                                                gtTACAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAACGACAAG‑C‑ACA‑TTTTTTTTTTCCTCATC‑TG‑TTTTTAGATG‑CGAAACATG‑AAA‑ATT‑AAAGGAGGGAAAAAAAAAGGGGA‑TTATTGGGTAtt                                                         >  2:210158/1‑151 (MQ=255)
                                                                                       cTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAGACAAG‑C‑ACA‑ATTTTTTTTTCCTCATC‑TT‑TTTTTAAATG‑CGAAAAAAG‑AAA‑ATC‑AAACGAGGAAAAAAAAAAGGGGA‑AGGTTTGCTAtttttccga                                                  >  2:254629/1‑146 (MQ=255)
                                                                                         ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAACGGCAAG‑C‑ACA‑TTTTTTTTTTTCTCCTC‑TT‑TTTTTAAATG‑CGAAAAAAG‑AAA‑ATT‑AAAGGGGGGAAAAAAAATGGGGA‑TGATTTGGTATTTTTTAGaaa                                                >  2:631882/1‑151 (MQ=255)
                                                                                         ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAAACACAAA‑A‑AAA‑ATTTTTTTTTTCTCCTCTTG‑TTTTAAAAAG‑GAAAAATTA‑AAA‑TTT‑AAC‑GAGGGAAAAAAAATGGGGA‑TGGTTTGCTCTTTTTTAGaaa                                                >  1:476220/1‑151 (MQ=255)
                                                                                           gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAACGACATGCC‑AAA‑TTTTTTTTTCCC‑CCTC‑TG‑TTTTTAAAAG‑CGAAACAAG‑AAA‑ATT‑AAAGGCGGGAAAATAAAATGGGA‑TTATTGGGtttttttttgaaaat                                              >  2:509750/1‑142 (MQ=255)
                                                                                           gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCACAAAAAACGGCCAG‑A‑ACA‑TTTTTTTTTTCCTCCAC‑TT‑TTTTTAAATG‑CCAAACAAG‑CAA‑ATT‑AACCGGGGGAAAACAAATGGGAATTGTTTGCTTTTTTTGCAGaatt                                               >  2:1626600/1‑149 (MQ=255)
                                                                                            ttATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAAAGACATG‑C‑ACA‑TTTTTTTTTTCCCCCTC‑TT‑TTTTTAAATG‑CGAAAAAAA‑AAA‑ATT‑AAAGGGGGGAAAAAAAATGGGGA‑TGATTTGGTATTTTTTAGaaaatt                                             >  2:167729/1‑148 (MQ=255)
                                                                                            ttATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTg                                             <  2:1524046/151‑1 (MQ=255)
                                                                                            ttATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAGACAAG‑C‑ACA‑TTTTATTTTTTCTCATC‑TG‑TTTTTAGAAT‑CGAAACATG‑AAA‑ATT‑AAAAGAGGGAAAAAAAATAGGGA‑TGATTGGGTAtttttgtgaaaatt                                             >  1:371040/1‑141 (MQ=255)
                                                                                              ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGAc                                           <  1:156512/151‑1 (MQ=255)
                                                                                              ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGAc                                           <  2:1421919/151‑1 (MQ=255)
                                                                                              ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GTCAGAAAAAAAGAAAAG‑A‑ACA‑TTTTTTTTTTTCTTCTC‑TG‑TTTTTAAAAG‑GGGAACATT‑AAA‑TTT‑TAAGGGGGAAAAAAAAAATGGAA‑TGATTTGGTATTTTTCAGGAAATGaa                                           >  1:111093/1‑150 (MQ=255)
                                                                                                acaacaGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTc                                         <  2:146727/151‑1 (MQ=255)
                                                                                                 caacaGTAAATAATAAAAATTTCTCGAGGCGGG‑TTCAGAAAAAAAGACAAG‑T‑ACA‑TTTTATTTTTCCTCCTC‑TG‑TTTTTAGAAG‑AGAAAAAAG‑AAA‑ATCAAACGGGGGAAAAA‑AAATGGGAA‑AGATTGGGCATTTTTTAGAAAATGAATCa                                        >  2:1048724/1‑151 (MQ=255)
                                                                                                   acaGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAAAGACATG‑C‑ACA‑TTTTTTTTTTCCTCCTC‑TG‑TTTTTAAATG‑GGAAAAATT‑AAATTTA‑AAGGGGGGAAAAAAAAATGGGAA‑TGTTTGGTTTTTTTTGAAAAAttaatcaaa                                       >  2:736035/1‑144 (MQ=255)
                                                                                                   acaGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCCGAAAAAAAGAAAAG‑G‑ACA‑TTTTTTTTTTCCTCCTC‑TG‑TTTTTTGATG‑CGAAAAAAG‑AAA‑ATT‑AAAGGAGGGAAAAAAAAAAGGGA‑TGATTTGGTATTTTTTAGaaaattaatcaaa                                      >  1:1540800/1‑141 (MQ=255)
                                                                                                    caGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAACGAAATG‑A‑ACA‑TTTTTTTTTACCCCCTC‑TG‑TTTTTAAAAT‑CGAAACAAG‑AAATTTT‑AAGGGGGGAAAATAAAATGGGAA‑‑GATTGGCACTTTTTTTGAAAATTAATCAGaa                                     >  2:319310/1‑151 (MQ=255)
                                                                                                       tAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAg                                  <  1:1634283/151‑1 (MQ=255)
                                                                                                         aataatAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGAt                                <  2:1226390/151‑1 (MQ=255)
                                                                                                          ataataAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGAtt                               <  2:1215076/151‑1 (MQ=255)
                                                                                                                   aTTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTg                      <  2:530227/151‑1 (MQ=255)
                                                                                                                   aTTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTg                      <  1:332239/151‑1 (MQ=255)
                                                                                                                             gCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTc              <  1:962302/149‑1 (MQ=255)
                                                                                                                             gCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTc              <  2:895144/149‑1 (MQ=255)
                                                                                                                             gCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCt             <  1:150458/150‑1 (MQ=255)
                                                                                                                                 g‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGa        >  1:112903/1‑151 (MQ=255)
                                                                                                                                    ccAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaa      <  1:1206328/151‑1 (MQ=255)
                                                                                                                                      aGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaaaa    >  2:1285954/1‑151 (MQ=255)
                                                                                                                                       gAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaaaa    >  2:41577/1‑150 (MQ=255)
                                                                                                                                        aaaaaGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAAcc  <  1:1271614/151‑1 (MQ=255)
                                                                                                                                        aaaaaGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAAc   <  1:1732570/150‑1 (MQ=255)
                                                                                                                                        aaaaaGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAAc   <  1:771263/150‑1 (MQ=255)
                                                                                                                                        aaaaaGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAAc   <  2:257753/150‑1 (MQ=255)
                                                                                                                                         aaaaGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAAc   >  2:448602/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                           
ATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATG‑C‑ACA‑TTTTATTTTCCCTCATC‑TG‑TTTTAAGATG‑CGAAACATG‑AAA‑TTC‑AACGGAGGAAAAATAAATGGGAA‑TGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACC  >  NC_000913/2386722‑2387007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑