Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,763,869 (T)7→8 coding (1285/1617 nt) yfjL ← CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,763,8621.T100.0% 86.8 / NA 26I431N (ATC→AAC) yfjLCP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (13/13);  total (13/13)

TTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGA  >  NC_000913/2763736‑2763984
                                                                                                                               |                                                                                                                          
ttAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttttttttgcgc                                                                                                                >  2:349343/1‑134 (MQ=255)
 tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTgcgcg                                                                                                                >  1:355591/1‑139 (MQ=255)
 tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTgcgcg                                                                                                                <  2:355591/139‑1 (MQ=255)
    agTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCgg                                                                                                             >  1:211539/1‑139 (MQ=255)
      tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTgcgcg                                                                                                                <  2:20437/134‑1 (MQ=255)
      tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTgcgcg                                                                                                                >  1:20437/1‑134 (MQ=255)
           gCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGAt                                                                                                      >  1:295531/1‑139 (MQ=255)
                         caAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGt                                                                                        <  1:349343/139‑1 (MQ=255)
                                       tCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGa                                                                          >  1:62339/1‑139 (MQ=255)
                                        cGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttttttttgcgc                                                                                                                <  1:174392/99‑6 (MQ=255)
                                        cGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttttttttgcgc                                                                                                                >  2:174392/1‑94 (MQ=255)
                                                 cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGaa                                                                                            >  1:346419/1‑111 (MQ=255)
                                                 cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGaa                                                                                            <  2:346419/111‑1 (MQ=255)
                                                                tGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACgg                                                 <  2:53183/139‑1 (MQ=255)
                                                                  ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa                                               <  2:62339/139‑1 (MQ=255)
                                                                  ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa                                               <  2:295531/139‑1 (MQ=255)
                                                                            tttCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAatat                                     >  1:586140/1‑139 (MQ=255)
                                                                                                 gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc                <  2:65145/139‑1 (MQ=255)
                                                                                                       aGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc                >  1:388390/1‑133 (MQ=255)
                                                                                                       aGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc                <  2:388390/133‑1 (MQ=255)
                                                                                                              gTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCa                                   >  1:434123/1‑107 (MQ=255)
                                                                                                              gTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCa                                   <  2:434123/107‑1 (MQ=255)
                                                                                                               tCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGa  >  2:51926/1‑139 (MQ=255)
                                                                                                                   tAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATg                                                                                                     <  2:261187/36‑1 (MQ=255)
                                                                                                                   tAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATg                                                                                                     >  1:261187/1‑36 (MQ=255)
                                                                                                                         cGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCt                                                                                     <  2:185551/46‑1 (MQ=255)
                                                                                                                         cGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCt                                                                                     >  1:185551/1‑46 (MQ=255)
                                                                                                                          gAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa                                               >  2:226230/1‑83 (MQ=255)
                                                                                                                          gAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa                                               <  1:226230/83‑1 (MQ=255)
                                                                                                                               |                                                                                                                          
TTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGA  >  NC_000913/2763736‑2763984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: