Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,126,422	C→T	A257T (GCG→ACG)	wcaG ←	bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,126,422	0	C	T	100.0%	111.1 / NA	33	A257T (GCG→ACG)	wcaG	bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/14);  total (19/14)

CCAGTGAGATTTCGTGATACCAGCCAAGCTGATGCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACG  >  NC_000913/2126287‑2126552                                                                                                                                        |                                                                                                                                   ccAGTGAGATTTCGTGATACCAGCCAAGCTGATGCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAg                                                                                                                                 <  2:231641/139‑1 (MQ=255)              gTGATGCCAGCCAAGCTGATGCAGGCGCGTCGCATCCAGCAGTTTGCGCGGTGTGCCATCCGGTTTGCAGGCATCAAAAACCACCCGGCCTTTGTAACCCTCCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTg                                                                                                                    <  2:112175/139‑1 (MQ=255)               tGATACCAGCCAAGCTGATGCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGc                                                                                                                   >  2:276017/1‑139 (MQ=255)                   aCCAGCCAAGCTGATGCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTc                                                                                                               >  2:79949/1‑139 (MQ=255)                     cAGCCAAGCTGATGCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGa                                                                                                                         >  1:476839/1‑127 (MQ=255)                     cAGCCAAGCTGATGCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGa                                                                                                                         <  2:476839/127‑1 (MQ=255)                            gCTGATGCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGt                                                                                                      >  2:55115/1‑139 (MQ=255)                                  gCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGAc                                                                                                >  1:259087/1‑139 (MQ=255)                                      gcgcGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTa                                                                                            <  1:510598/139‑1 (MQ=255)                                          gTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGa                                                                                                                         <  1:33931/106‑1 (MQ=255)                                          gTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGa                                                                                                                         >  2:33931/1‑106 (MQ=255)                                          gTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGCCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAAtgt                                                                                        >  2:621915/1‑139 (MQ=255)                                           tCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAAtgtg                                                                                       <  1:125896/139‑1 (MQ=255)                                                      agTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCg                                                                            <  1:262966/139‑1 (MQ=255)                                                        tttGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGc                                                                          >  1:571009/1‑139 (MQ=255)                                                        tttGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGc                                                                          >  2:444459/1‑139 (MQ=255)                                                         ttGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGa                                                                                                                         >  2:321111/1‑91 (MQ=255)                                                         ttGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGa                                                                                                                         <  1:321111/91‑1 (MQ=255)                                                                         ccGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTccagcca                                                         >  2:492079/1‑139 (MQ=255)                                                                                           aaaCCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTc                                       <  1:131805/139‑1 (MQ=255)                                                                                             accaccCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCca                                     <  1:276017/139‑1 (MQ=255)                                                                                                accCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCcatga                                  >  1:81179/1‑139 (MQ=255)                                                                                                  ccGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGATAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCcatgaca                                >  2:456253/1‑139 (MQ=255)                                                                                                                   ccacTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCa                                           >  1:626567/1‑111 (MQ=255)                                                                                                                   ccacTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCa                                           <  2:626567/111‑1 (MQ=255)                                                                                                                   ccacTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTcgccgccgc               <  2:571009/139‑1 (MQ=255)                                                                                                                   ccacTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTcgccgccgc               >  2:593874/1‑139 (MQ=255)                                                                                                                    cacTTTGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCgccgccgcc              <  1:55115/139‑1 (MQ=255)                                                                                                                      ccttGGCGATGGTTTGCGTCATCCCGGGGATAGTGCAGTTAACGCCCGTGCCGACGTCGATGTGCGACAACATGGTCTGGGTGTTCTCCTTCCAGCCTTCATGCGCCACCTCCATGCCATGACTGCTCGCCGCCGCCat            >  2:417250/3‑139 (MQ=255)                                                                                                                         tGGCGATGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGTCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATatc         >  2:161256/1‑139 (MQ=255)                                                                                                                              aTGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGa    >  2:584322/1‑139 (MQ=255)                                                                                                                               tGGTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGAc   <  1:444459/139‑1 (MQ=255)                                                                                                                                ggTTTGCGTCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACg  >  2:483114/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                   CCAGTGAGATTTCGTGATACCAGCCAAGCTGATGCAGGCGCGTCACATCCAGCAGTTTGCGCGGCGTGCCATCCGGTTTGCTGGCATCAAAAACCACCCGGCCTTTGTAACCCACCACTTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACG  >  NC_000913/2126287‑2126552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑