Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,472	A→G	E498G (GAG→GGG)	yjcE →	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,472	0	A	G	100.0%	95.3 / NA	26	E498G (GAG→GGG)	yjcE	putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

AGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTT  >  NC_000913/4281365‑4281607                                                                                                            |                                                                                                                                        agagAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCa                                                                                                          >  1:478639/1‑139 (MQ=255)   agaATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt                                                                                                        >  1:456868/1‑139 (MQ=255)     aaTATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAg                                                                                                                               >  1:226451/1‑114 (MQ=255)     aaTATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAg                                                                                                                               >  1:237137/1‑114 (MQ=255)     aaTATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAg                                                                                                                               <  2:237137/114‑1 (MQ=255)     aaTATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAg                                                                                                                               <  2:226451/114‑1 (MQ=255)               aCCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACg                                                                                            >  1:354003/1‑139 (MQ=255)                   gctgctTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCt                                                                                        <  2:97529/139‑1 (MQ=255)                     tgctTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGa                                                                                      >  1:625674/1‑139 (MQ=255)                                       ctcGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc                                                                    <  1:318868/139‑1 (MQ=255)                                          gcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc                                                                    >  1:514965/1‑136 (MQ=255)                                          gcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc                                                                    <  2:514965/136‑1 (MQ=255)                                          gcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcg                                                                     <  1:125311/135‑1 (MQ=255)                                          gcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcg                                                                     >  2:125311/1‑135 (MQ=255)                                               aTTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcg                                                                       >  1:494071/1‑128 (MQ=255)                                               aTTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcg                                                                       <  2:494071/128‑1 (MQ=255)                                                    tAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATc                                                       <  1:343915/139‑1 (MQ=255)                                                           cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                                >  1:615638/1‑139 (MQ=255)                                                           cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                                >  2:69335/1‑139 (MQ=255)                                                                                 acgacgTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCa                          <  2:615638/139‑1 (MQ=255)                                                                                 acgacgTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCa                          <  2:625674/139‑1 (MQ=255)                                                                                      gTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGAtc                     >  2:206071/1‑139 (MQ=255)                                                                                           aaGTTCCGTGCAGGAAGGGAACCTTGTGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCGCGATCTCGAt                <  2:456868/139‑1 (MQ=255)                                                                                                   tGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTg         >  1:288020/1‑138 (MQ=255)                                                                                                   tGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTg         <  2:288020/138‑1 (MQ=255)                                                                                                         aaGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGtt  <  2:478639/139‑1 (MQ=255)                                                                                                            |                                                                                                                                        AGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTT  >  NC_000913/4281365‑4281607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑