Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,026,864	A→G	L51S (TTA→TCA)	mgsA ←	methylglyoxal synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,026,864	0	A	G	100.0%	193.1 / NA	51	L51S (TTA→TCA)	mgsA	methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/32);  total (19/32)

GTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTA  >  NC_000913/1026739‑1026993                                                                                                                              |                                                                                                                                  gTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGt                                                                                                                      <  1:91326/139‑1 (MQ=255)   ggATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCTCTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGc                                                                                                                    <  2:587356/139‑1 (MQ=255)   ggATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGc                                                                                                                    <  2:285606/139‑1 (MQ=255)   ggATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGc                                                                                                                    <  1:591042/139‑1 (MQ=255)   ggATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGc                                                                                                                    <  1:589922/139‑1 (MQ=255)   ggATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGc                                                                                                                    <  2:148478/139‑1 (MQ=255)   ggATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGc                                                                                                                    <  1:272714/139‑1 (MQ=255)       cccAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGt                                                                                                                >  2:135606/1‑139 (MQ=255)        ccAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGtt                                                                                                               <  1:135606/139‑1 (MQ=255)         cAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTg                                                                                                              <  2:568119/139‑1 (MQ=255)            aagaaAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGt                                                                                                                      <  1:444225/128‑1 (MQ=255)            aagaaAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGt                                                                                                                      >  2:444225/1‑128 (MQ=255)            aagaaAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCAt                                                                                                           <  2:462535/139‑1 (MQ=255)                 aaTCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGtt                                                                                                               >  2:104271/1‑130 (MQ=255)                 aaTCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGtt                                                                                                               <  1:104271/130‑1 (MQ=255)                 aaTCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGt                                                                                                      <  2:458388/139‑1 (MQ=255)                 aaTCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGt                                                                                                      >  2:176946/1‑139 (MQ=255)                      aTACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACgtg                                                                                                 >  1:259662/1‑139 (MQ=255)                            cAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTTGTGCCTGTTGCATACAGTACGTGTTGTTc                                                                                           >  2:260667/1‑139 (MQ=255)                                           agaTCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGc                                                                                                                    >  2:148241/1‑99 (MQ=255)                                           agaTCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGc                                                                                                                    <  1:148241/99‑1 (MQ=255)                                           agaTCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATg                                                                            <  1:25289/139‑1 (MQ=255)                                           agaTCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATg                                                                            <  1:176946/139‑1 (MQ=255)                                                              ctgGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATg                                                                            >  1:520574/1‑120 (MQ=255)                                                              ctgGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATg                                                                            <  2:520574/120‑1 (MQ=255)                                                                   cACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTg                                                                                                                 >  2:530476/1‑78 (MQ=255)                                                                   cACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTg                                                                                                                 <  1:530476/78‑1 (MQ=255)                                                                      cccccATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTgttgtt                                                                                            <  1:290646/96‑1 (MQ=255)                                                                      cccccATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTgttgtt                                                                                            >  2:290646/1‑96 (MQ=255)                                                                                 ccACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTTGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGAt                                      <  1:260667/139‑1 (MQ=255)                                                                                 ccACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGAt                                      <  1:276442/139‑1 (MQ=255)                                                                                 ccACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGAt                                      <  1:14829/139‑1 (MQ=255)                                                                                    cTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTa                                                                                                                              <  1:443272/48‑1 (MQ=255)                                                                                    cTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTa                                                                                                                              >  2:443272/1‑48 (MQ=255)                                                                                           tCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTATCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGcccc                            <  2:381584/139‑3 (MQ=255)                                                                                            cgcgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGccacc                           >  2:234303/1‑139 (MQ=255)                                                                                            cgcgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGGGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGccacc                           <  1:531543/139‑1 (MQ=255)                                                                                              cgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGcc                                                                          >  1:597875/1‑90 (MQ=255)                                                                                              cgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGcc                                                                          <  2:597875/90‑1 (MQ=255)                                                                                              cgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCtc                                                            <  1:40492/104‑1 (MQ=255)                                                                                              cgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCtc                                                            >  2:40492/1‑104 (MQ=255)                                                                                              cgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCa                                           >  2:623835/1‑121 (MQ=255)                                                                                              cgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCa                                           <  1:623835/121‑1 (MQ=255)                                                                                              cgTTGACGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAg                         >  1:182747/1‑139 (MQ=255)                                                                                                   aCGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTa                                                                                                                        <  2:284977/39‑1 (MQ=255)                                                                                                   aCGTTCATGCCGGTCGCGCGGGAAATTGAGTTACCGGTa                                                                                                                        >  1:284977/1‑39 (MQ=255)                                                                                                             cGGTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCcg          <  2:231991/139‑1 (MQ=255)                                                                                                               gTCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCcgcg        <  1:58233/139‑1 (MQ=255)                                                                                                                tCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAg                                                                                                       >  2:207307/1‑43 (MQ=255)                                                                                                                tCGCGCGGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAg                                                                                                       <  1:207307/43‑1 (MQ=255)                                                                                                                     cgGGAAATTGAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTa  >  1:164496/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                  GTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTA  >  NC_000913/1026739‑1026993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑