Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,028,652	C→T	V24M (GTG→ATG)	hspQ ←	heat shock protein involved in degradation of mutant DnaA, hemimethylated oriC DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,028,652	0	C	T	100.0%	128.7 / NA	37	V24M (GTG→ATG)	hspQ	heat shock protein involved in degradation of mutant DnaA, hemimethylated oriC DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/19);  total (18/19)

GTATGAACCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCTTTGATATAAGGGTAAAATTCCACATTTCAAGT  >  NC_000913/1028525‑1028779                                                                                                                                |                                                                                                                                gtaTGAACCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGAc                                                                                                                              >  1:604461/1‑131 (MQ=255) gtaTGAACCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGAc                                                                                                                              <  2:604461/131‑1 (MQ=255)   aTGAACCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCgaga                                                                                                                    <  2:173163/139‑1 (MQ=255)       aCCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCgaga                                                                                                                    <  2:597049/135‑1 (MQ=255)       aCCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCgaga                                                                                                                    >  1:597049/1‑135 (MQ=255)            tAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAAc                                                                                                           >  2:13529/1‑139 (MQ=255)                              cATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGAc                                                                                                                              >  2:395119/1‑102 (MQ=255)                              cATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGAc                                                                                                                              <  1:395119/102‑1 (MQ=255)                              cATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctgc                                                                                         >  1:292777/1‑139 (MQ=255)                                tCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctgct                                                                                        >  2:583024/1‑138 (MQ=255)                                tCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACctgct                                                                                        <  1:583024/138‑1 (MQ=255)                                          aTACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCg                                                                             >  2:474054/1‑139 (MQ=255)                                           tACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCga                                                                                                                      >  2:45550/1‑97 (MQ=255)                                           tACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCga                                                                                                                      <  1:45550/97‑1 (MQ=255)                                                cGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTg                                                                       >  2:422469/1‑139 (MQ=255)                                                cGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTg                                                                       >  1:216365/1‑139 (MQ=255)                                                cGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTg                                                                       <  1:422469/139‑1 (MQ=255)                                                  gAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACTTGCTGGc                                                                                     >  2:193772/1‑123 (MQ=255)                                                  gAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGc                                                                                     <  1:193772/123‑1 (MQ=255)                                                    gcggcgCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTgg                                                                   >  1:363198/1‑139 (MQ=255)                                                    gcggcgCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTgg                                                                   <  2:292777/139‑1 (MQ=255)                                                    gcggcgCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTAGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCGGATACCGAATTTGCTgg                                                                   <  2:363198/139‑1 (MQ=255)                                                              gCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGt                                                         <  1:13529/139‑1 (MQ=255)                                                                 cGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCAc                                                      <  1:327507/139‑1 (MQ=255)                                                                         aCCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCggg                                              >  1:472131/1‑139 (MQ=255)                                                                         aCCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCggg                                              >  1:273177/1‑139 (MQ=255)                                                                                 cTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGt                                                         >  1:601766/1‑120 (MQ=255)                                                                                 cTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGt                                                         <  2:601766/120‑1 (MQ=255)                                                                                      caGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCtt                                 <  2:472131/139‑1 (MQ=255)                                                                                      caGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCtt                                 <  1:79735/139‑1 (MQ=255)                                                                                      caGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCtt                                 <  2:273177/139‑1 (MQ=255)                                                                                      caGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCtt                                 <  2:216365/139‑1 (MQ=255)                                                                                                       agCGAATAAACCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCTTTGATATAAGGGTAAAAt                >  1:601509/1‑139 (MQ=255)                                                                                                               aaCCGGGTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCTTTGATATAAGGGTAAAATTCCACAtt        <  1:474054/139‑1 (MQ=255)                                                                                                                     gTCGATATCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCTTTGATATAAGGGTAAAATTCCACATTTCAAGt  >  1:372337/1‑139 (MQ=255)                                                                                                                          tatCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCtt                                 <  2:246346/103‑1 (MQ=255)                                                                                                                          tatCCATGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCtt                                 >  1:246346/1‑103 (MQ=255)                                                                                                                                |                                                                                                                                GTATGAACCGGTAGGCCGTTATCGTCCTCCATCACTACGTGATACCACGGAGCGGCGCGAAGCTCGTCATTCACCGCCAGCTCATCAGGCGACGGTTCAGAGAGCGAATAAACCGGGTCGATATCCACGACCACTCCGAGATAACCTAACAGGGAATGGCGGACCTGCTGGCCGATACCGAATTTGCTGGCAATCATAGTCACCTCCCGGGAAATCTTTACCTTTGATATAAGGGTAAAATTCCACATTTCAAGT  >  NC_000913/1028525‑1028779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑