Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,228,320	T→C	L81P (CTG→CCG)	ycgM →	putative isomerase/hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,228,320	0	T	C	100.0%	117.5 / NA	32	L81P (CTG→CCG)	ycgM	putative isomerase/hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/19);  total (13/19)

CAGTGCTGTTTATTAAACCAGAAACGGCACTGTGCGATCTGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACTGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTT  >  NC_000913/1228200‑1228450                                                                                                                         |                                                                                                                                   cAGTGCTGTTTATTAAACCAGAAACGGCACTGTGCGATCTGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAaga                                                                                                                  >  2:191405/1‑139 (MQ=255)                   cAGAAACGACACTGTGCGATCTGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCAt                                                                                                >  1:135921/1‑139 (MQ=255)                                gtgCGATCTGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGc                                                                                             >  1:327765/1‑129 (MQ=255)                                gtgCGATCTGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGc                                                                                             <  2:327765/129‑1 (MQ=255)                                      tCTGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTAtggtgtggcg                                                                             <  1:191405/139‑1 (MQ=255)                                        tGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCt                                                                           <  2:493224/139‑1 (MQ=255)                                        tGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCt                                                                           <  2:437156/139‑1 (MQ=255)                                         gCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGgcgc                                                                            >  1:15603/1‑137 (MQ=255)                                         gCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGgcgc                                                                            <  2:15603/137‑1 (MQ=255)                                          cGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCt                                                                           <  1:223983/137‑1 (MQ=255)                                          cGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCt                                                                           >  2:223983/1‑137 (MQ=255)                                          cGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGGGGCGCTCg                                                                         <  2:135921/139‑1 (MQ=255)                                           ggCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGc                                                                                             >  1:86310/1‑118 (MQ=255)                                           ggCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGc                                                                                             <  2:86310/118‑1 (MQ=255)                                           ggCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGa                                                                        >  2:92546/1‑139 (MQ=255)                                           ggCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGa                                                                        >  2:306197/1‑139 (MQ=255)                                             cAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATc                                                                      <  2:464095/139‑1 (MQ=255)                                               gCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTg                                                                    <  1:454344/139‑1 (MQ=255)                                                  aTTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACa                                                                 <  1:455349/139‑1 (MQ=255)                                                    tGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACAtt                                                               <  1:30969/139‑1 (MQ=255)                                                          tCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCt                                                                           <  1:627430/121‑1 (MQ=255)                                                          tCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCt                                                                           >  2:627430/1‑121 (MQ=255)                                                                               ttcaTCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATgaagaa                                    <  2:109209/139‑1 (MQ=255)                                                                                    catGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTAtggcgtggcg                                                                             <  2:629174/93‑1 (MQ=255)                                                                                    catGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTAtggcgtggcg                                                                             >  1:629174/1‑93 (MQ=255)                                                                                     atGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCgg                              <  1:92546/139‑1 (MQ=255)                                                                                         aGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAg                          >  1:539019/1‑139 (MQ=255)                                                                                         aGTCGAACTGGCGGTGTTGATTGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTAATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAg                          >  2:456209/1‑139 (MQ=255)                                                                                                              tGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCg     <  1:106312/139‑1 (MQ=255)                                                                                                              tGGCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCg     <  2:539019/139‑1 (MQ=255)                                                                                                               ggCGCGACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGt    <  1:306197/139‑1 (MQ=255)                                                                                                                 cgcgACACCGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGttt  >  2:309305/1‑139 (MQ=255)                                                                                                                         |                                                                                                                                   CAGTGCTGTTTATTAAACCAGAAACGGCACTGTGCGATCTGCGGCAGCCATTGGCGATCCCATCCGATTTCGGTTCAGTTCATCATGAAGTCGAACTGGCGGTGTTGATTGGCGCGACACTGCGTCAGGCTACGGAAGAGCATGTCCGCAAAGCCATTGCCGGTTATGGCGTGGCGCTCGATCTGACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTT  >  NC_000913/1228200‑1228450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑