Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 705,972 (T)9→8 intergenic (+82/‑121) nagE → / → glnS N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913705,9640T.100.0% 34.1 / NA 9intergenic (+74/‑129)nagE/glnSN‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/5);  total (4/5)

CAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACG  >  NC_000913/705840‑706092
                                                                                                                            |                                                                                                                                
cAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTCatcaat                                                                                                                   <  1:432283/139‑1 (MQ=255)
                                                                 gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCGGCCTGTCCCGCtt                                                  >  2:354908/1‑139 (MQ=255)
                                                                              aGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACg                                     <  2:168509/139‑1 (MQ=255)
                                                                                                 aCAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTCATCAatcat                                                                                                                <  1:425141/45‑1 (MQ=255)
                                                                                                 aCAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTCATCAatcat                                                                                                                >  2:425141/1‑45 (MQ=38)
                                                                                                       ggTGGAGATCTTCTCTGCCGCTTTTTTTTCATCAatcat                                                                                                                <  1:41089/39‑1 (MQ=255)
                                                                                                       ggTGGAGATCTTCTCTGCCGCTTTTTTTTCATCAatcat                                                                                                                >  2:41089/1‑39 (MQ=37)
                                                                                                        gTGGAGATCTTCTCTGCCGCTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAg           <  2:271417/139‑1 (MQ=255)
                                                                                                                 ttctCTGCCGCTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACg  >  1:202416/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                
CAGGGCCATATTGTGGCGGGTCAAACACCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTTTTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGCCGTTATACGTTGTTTACGCTTTGAGGAATCCACG  >  NC_000913/705840‑706092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: