Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,124,562	(T)5→6	coding (898/1224 nt)	glcF ←	glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,124,557	1	.	T	100.0%	18.8 / NA	7	K301K (AAG→AAA)	glcF	glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (4/3);  total (4/3)

GGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGC‑TTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCA  >  NC_000913/3124448‑3124686                                                                                                               |                                                                                                                                  ggCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTTATCGCCGCGAATTGCCAGttttt                                                                                                       >  1:55518/1‑139 (MQ=255)                        gTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGt                                                                                >  1:204913/1‑139 (MQ=255)                                  gACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAAt                                                                      >  1:374453/1‑139 (MQ=255)                                              cTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGtt                                                          <  2:204913/139‑1 (MQ=255)                                                     cACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCActgac                                                   <  2:374453/139‑1 (MQ=255)                                                                                          cacGGACAGTGGAAGGCCAGCTTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTc              >  2:289423/1‑139 (MQ=255)                                                                                                      aaGGCCAGCTTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCa  <  2:394845/139‑1 (MQ=255)                                                                                                               |                                                                                                                                  GGCTGTCGGGAACGTCCGTTAAGGTAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGC‑TTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCA  >  NC_000913/3124448‑3124686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑