Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	506,758	(T)6→7	coding (325/480 nt)	ybaK ←	Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	506,752	1	.	T	100.0%	31.4 / NA	11	R111S (CGT→AGT)	ybaK	Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (5/6);  total (5/6)

GCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑TTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCG  >  NC_000913/506625‑506822                                                                                                                                 |                                                                       gCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑tttttttctgc                                                             >  2:414026/1‑134 (MQ=255)     aTTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCa                                                          <  2:98848/139‑1 (MQ=255)      tttGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAg                                                         >  1:126907/1‑139 (MQ=255)          gCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTggg                                                     <  2:123008/139‑1 (MQ=255)                        tGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATccc                                             >  1:106837/1‑133 (MQ=255)                        tGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATccc                                             <  2:106837/133‑1 (MQ=255)                              aTCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTa                                 >  2:89115/1‑139 (MQ=255)                                         ccAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAAc                      <  1:374124/139‑1 (MQ=255)                                               cGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTgcg                <  1:414026/139‑1 (MQ=255)                                                  tatCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑tttttttctg                                                              >  1:139449/1‑85 (MQ=255)                                                  tatCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑tttttttctg                                                              <  2:139449/89‑5 (MQ=255)                                                            gcgcTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATcccc                                            >  1:134531/1‑98 (MQ=255)                                                            gcgcTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATcccc                                            <  2:134531/98‑1 (MQ=255)                                                             cgcTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATACCGTCGAACGCTGCGCGACCatcggatcg  >  2:71902/1‑139 (MQ=255)                                                                       cgGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑tttttttctg                                                              >  1:389005/1‑64 (MQ=255)                                                                       cgGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑tttttttctg                                                              <  2:389005/68‑5 (MQ=255)                                                                                                                                 |                                                                       GCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑TTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCG  >  NC_000913/506625‑506822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑