Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,415,887 (G)6→7 coding (1613/1626 nt) aidB → DNA alkylation damage repair protein, flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,415,8811.G100.0% 81.0 / NA 22T536R (ACG→AGG) aidBDNA alkylation damage repair protein, flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (15/7);  total (15/7)

CCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAG  >  NC_000913/4415744‑4416009
                                                                                                                                          |                                                                                                                                
ccTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACg                                                                                                                                 >  1:203753/1‑139 (MQ=255)
 ctgctgGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACgg                                                                                                                                <  1:96554/139‑1 (MQ=255)
        gCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACggggggga                                                                                                                          >  2:255782/1‑136 (MQ=255)
        gCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACggggggga                                                                                                                          <  1:255782/138‑3 (MQ=255)
           gCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgt                                                                                                                       <  1:165945/139‑1 (MQ=255)
                           gAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATAc                                                                                                       >  1:60572/1‑139 (MQ=255)
                                  gCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACggggggga                                                                                                                          >  2:34443/1‑110 (MQ=255)
                                  gCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACggggggga                                                                                                                          <  1:34443/112‑3 (MQ=255)
                                            cAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTgg                                                                                      <  2:203753/139‑1 (MQ=255)
                                                   gcAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGAt                                                                               <  2:60572/139‑1 (MQ=255)
                                                                       tGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTaa                                                                                                               <  2:6653/87‑1 (MQ=255)
                                                                       tGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTaa                                                                                                               >  1:6653/1‑87 (MQ=255)
                                                                        gTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGaa                                                          >  1:251223/1‑139 (MQ=255)
                                                                                 gcgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAgg                                                 >  1:73352/1‑139 (MQ=255)
                                                                                 gcgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAgg                                                 >  1:242450/1‑139 (MQ=255)
                                                                                 gcgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACgggggggagt                                                                                                                        >  1:62770/1‑63 (MQ=255)
                                                                                 gcgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACgggggggagt                                                                                                                        <  2:62770/67‑5 (MQ=255)
                                                                                  cgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGa                                                                                                 <  1:361581/90‑1 (MQ=255)
                                                                                  cgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGa                                                                                                 >  2:361581/1‑90 (MQ=255)
                                                                                  cgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGc                                                >  1:57746/1‑139 (MQ=255)
                                                                                    cggcggcgTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCg                                              >  1:386353/1‑139 (MQ=255)
                                                                                                 ttGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTGAGCGTATACGACTGATGCGACGCTGGTATCGACTAACTAAATGAAATATGTGAAAATTGTAGGCCGGCCAAGGCGCTcg                                 >  2:145423/1‑139 (MQ=255)
                                                                                                           aGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgtgtgtg                                                                                                                  >  1:398821/1‑48 (MQ=255)
                                                                                                           aGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgtgtgtg                                                                                                                  <  2:398821/48‑1 (MQ=255)
                                                                                                           aGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATcc                       >  1:313650/1‑139 (MQ=255)
                                                                                                                  gAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTg                >  2:168977/1‑139 (MQ=255)
                                                                                                                       aTTTATTGCTGCGGGCGACGGGGGGGAgtgtgtgtg                                                                                                                  <  1:227431/36‑1 (MQ=255)
                                                                                                                       aTTTATTGCTGCGGGCGACGGGGGGGAgtgtgtgtg                                                                                                                  >  2:227431/1‑36 (MQ=255)
                                                                                                                        tttATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAtt          >  1:254467/1‑139 (MQ=255)
                                                                                                                                tgcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAg  >  2:38652/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                
CCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAG  >  NC_000913/4415744‑4416009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: