| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 701,461 | (G)5→6 | coding (134/1221 nt) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 701,456 | 1 | . | G | 88.9% | 50.9 / 1.9 | 18 | coding (139/1221 nt) | nagC | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (7/9); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
CCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGC‑GGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCC > NC_000913/701319‑701585 | ccGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGC‑g < 1:328672/139‑1 (MQ=255) cGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGAGCGGCGGCCCACGGTGGAGGCCTGCTCATCAACTTCTTTGATCAGCCCGCATTCGATAAGCTGACGCGTAATTTTGGTTACACTGGCGGGGGGCAAGCTGGc > 1:290197/1‑139 (MQ=255) gTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCt < 1:95103/139‑1 (MQ=255) tGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCtt > 1:309700/1‑139 (MQ=255) tGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCgg < 2:416566/139‑1 (MQ=255) tGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCgaga < 2:309700/139‑1 (MQ=255) tGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCgaga < 2:164009/139‑1 (MQ=255) cgcgGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCgaga > 2:76985/1‑130 (MQ=255) cgcgGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCgaga < 1:76985/130‑1 (MQ=255) ctgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACt < 1:107977/117‑1 (MQ=255) ctgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCCAGATTGGCCCGTACt > 2:107977/1‑117 (MQ=255) cccGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCgaga > 2:252385/1‑84 (MQ=255) cccGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCgaga < 1:252385/84‑1 (MQ=255) aTAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTa > 1:44482/1‑84 (MQ=255) aTAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTa < 2:44482/84‑1 (MQ=255) cgTAATTTTGGTTACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTg > 2:331987/1‑139 (MQ=255) tACGCTGGCGGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGcc < 2:426443/139‑1 (MQ=255) gCTGGC‑GGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGcc > 2:202473/1‑135 (MQ=255) gCTGGC‑GGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGcc < 1:202473/135‑1 (MQ=255) | CCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGC‑GGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCC > NC_000913/701319‑701585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |