Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 28,072 C→T G260G (GGC→GGT rihC → ribonucleoside hydrolase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091328,0720CT100.0% 139.9 / NA 42G260G (GGC→GGTrihCribonucleoside hydrolase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/23);  total (19/23)

GTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAA  >  NC_000913/27943‑28207
                                                                                                                                 |                                                                                                                                       
gTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTAcc                                                                                                                                <  1:222670/139‑1 (MQ=255)
         cTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacg                                                                                                                       >  2:329886/1‑139 (MQ=255)
         cTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacg                                                                                                                       <  2:244391/139‑1 (MQ=255)
          tACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacga                                                                                                                      <  2:108470/139‑1 (MQ=255)
          tACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacga                                                                                                                      >  2:329638/1‑139 (MQ=255)
          tACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacga                                                                                                                      <  2:417769/139‑1 (MQ=255)
            ccGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacgacg                                                                                                                    <  1:399218/139‑1 (MQ=255)
               tAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACggtg                                                                                                                 <  1:16586/139‑1 (MQ=255)
                      aGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGata                                                                                                          >  1:217464/1‑139 (MQ=255)
                      aGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGata                                                                                                          >  1:58567/1‑139 (MQ=255)
                        tATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATc                                                                                                        >  2:116278/1‑139 (MQ=255)
                            cAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTTGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACg                                                                                                    <  1:100748/139‑1 (MQ=255)
                                  ggCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGcc                                                                                              >  2:244944/1‑139 (MQ=255)
                                   gCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacgacg                                                                                                                    <  1:133162/116‑1 (MQ=255)
                                   gCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacgacg                                                                                                                    >  2:133162/1‑116 (MQ=255)
                                              cACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagccag                                                                                  <  1:357519/139‑1 (MQ=255)
                                                       tGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacga                                                                                                                      >  1:118753/1‑94 (MQ=255)
                                                       tGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacga                                                                                                                      <  2:118753/94‑1 (MQ=255)
                                                              tCGCCTGGCTGGTGCGCCTGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagcc                                                                                    <  1:286645/121‑1 (MQ=255)
                                                              tCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACggtgg                                                                                                                >  1:321047/1‑93 (MQ=255)
                                                              tCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACggtgg                                                                                                                <  2:321047/93‑1 (MQ=255)
                                                              tCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagcc                                                                                    >  2:286645/1‑121 (MQ=255)
                                                              tCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGc                                                                  >  1:389326/1‑139 (MQ=255)
                                                                     gctggTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGAt                                                           <  1:329886/139‑1 (MQ=255)
                                                                                        ttCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacgac                                                                                                                     <  1:276078/62‑1 (MQ=255)
                                                                                        ttCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCacgac                                                                                                                     >  2:276078/1‑62 (MQ=255)
                                                                                             tctcAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCcagcag                                   >  1:393669/1‑139 (MQ=255)
                                                                                             tctcAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTAcagcag                                   >  2:294991/1‑139 (MQ=255)
                                                                                               tcAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgtg                                 <  1:244944/139‑1 (MQ=255)
                                                                                                cAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgtgg                                >  2:399397/1‑139 (MQ=255)
                                                                                                 aaaCCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgtggg                               <  1:329638/139‑1 (MQ=255)
                                                                                                       tGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCAt                                                                <  1:78353/100‑1 (MQ=255)
                                                                                                       tGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCAt                                                                >  2:78353/1‑100 (MQ=255)
                                                                                                              tgGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCa                                                                 >  1:75819/1‑92 (MQ=255)
                                                                                                              tgGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCa                                                                 <  2:75819/92‑1 (MQ=255)
                                                                                                              tgGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCt                  >  2:223551/1‑139 (MQ=255)
                                                                                                                gCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTgg                <  2:217464/139‑1 (MQ=255)
                                                                                                                            cAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGcgtcgt    <  1:116278/139‑1 (MQ=255)
                                                                                                                             aGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagccagc                                                                                 >  2:118897/1‑61 (MQ=255)
                                                                                                                             aGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagccagc                                                                                 <  1:118897/61‑1 (MQ=255)
                                                                                                                              gggTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTaa  <  2:389326/139‑1 (MQ=255)
                                                                                                                              gggTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTaa  <  1:223551/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                       
GTTTAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAA  >  NC_000913/27943‑28207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: