Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	942,993	A→G	T679A (ACG→GCG)	dmsA →	dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	942,993	0	A	G	84.2%	46.5 / ‑0.1	19	T679A (ACG→GCG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (6/10);  total (8/11)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

TTCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTGAAAGCGGCTTGCCGTCAGG  >  NC_000913/942858‑943125                                                                                                                                        |                                                                                                                                     ttCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACgcgc                                                                                                                                   >  1:143874/1‑139 (MQ=255)  tCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGcc                                                                                                                                  <  2:191398/139‑1 (MQ=255)  tCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCAGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACACGcc                                                                                                                                  >  1:435343/1‑139 (MQ=255)   ccGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGCCg                                                                                                                                 <  2:143874/139‑1 (MQ=255)            tCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTTCCGATCTACGCGCCGGGCTTTGaa                                                                                                                        <  2:309294/139‑1 (MQ=255)                     aaaCCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGCCGGGCTTTGaa                                                                                                                        >  2:372366/1‑130 (MQ=255)                     aaaCCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGCCGGGCTTTGaa                                                                                                                        <  1:372366/130‑1 (MQ=255)                      aaCCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGCCGGGCTTTGAAAGTTATCAgg                                                                                                              <  2:86517/139‑1 (MQ=255)                               aCTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGa                                                                                                     >  2:341705/1‑139 (MQ=255)                                   cGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGaacaa                                                                                                 >  1:212745/1‑139 (MQ=255)                                                  ttGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCa                                                                                  <  2:435343/139‑1 (MQ=255)                                                                                     gCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACt                                               <  2:212745/139‑1 (MQ=255)                                                                                               aaTTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACgg                                     <  1:341705/139‑1 (MQ=255)                                                                                                                   atcgatcCACTGCCGATCTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTg                                                                                    <  1:231194/72‑1 (MQ=255)                                                                                                                   atcgatcCACTGCCGATCTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTg                                                                                    >  2:231194/1‑72 (MQ=255)                                                                                                                        tcCACTGCCGATCTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTGAAAGCGGCt            <  1:179028/139‑1 (MQ=255)                                                                                                                                  ctCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACAATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTGAAAGCGGCTTGCCGTCAgg  >  2:235368/2‑139 (MQ=255)                                                                                                                                  aTCTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTGAAAGCGGCTTGCCGTCAgg  >  1:169029/1‑139 (MQ=255)                                                                                                                                    cTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTCCACt                                               >  1:270363/1‑92 (MQ=255)                                                                                                                                    cTACGCGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTCCACt                                               <  2:270363/92‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TTCCGTGAAGATCCGCAGGCAAACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTGAAAGCGGCTTGCCGTCAGG  >  NC_000913/942858‑943125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑