Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	40,651	A→G	S427S (TCT→TCC)	caiT ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	40,651	0	A	G	100.0%	96.6 / NA	26	S427S (TCT→TCC)	caiT	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATGGCGCGCGCCACACCGTACTGTTCGAT  >  NC_000913/40532‑40779                                                                                                                        |                                                                                                                                 ccgAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCg                                                                                                               >  2:277881/1‑139 (MQ=255) ccgAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCg                                                                                                               >  1:445413/1‑139 (MQ=255)        ccAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGt                                                                                                        <  2:30245/139‑1 (MQ=255)                    tACCGATAATGCCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTACAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCa                                                                                            <  1:277881/139‑1 (MQ=255)                                            aCCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATgaagaa                                                                                >  1:11832/1‑127 (MQ=255)                                            aCCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGAGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATgaagaa                                                                                <  2:11832/127‑1 (MQ=255)                                              cAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACAtggtgg                                                                  >  1:175446/1‑139 (MQ=255)                                                     tACGCACCAGCAGAGTTGGTTCTTCACCAACGCGTACTTCGCGGCAAGTGAACATCGGCAGGGTATCGGAGCAGGTGTTAACCTGCGTAACGGTGGCAATAAAGCGGAGGATGAAGAAGTCCCACATGGTGGCGGTGCt                                                           <  2:52948/139‑1 (MQ=255)                                                      aCGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTg                                                          <  1:404053/139‑1 (MQ=255)                                                           ccAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCa                                                                                            <  1:166173/100‑1 (MQ=255)                                                           ccAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCa                                                                                            >  2:166173/1‑100 (MQ=255)                                                               cagAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCag                                                                                           >  1:279231/1‑97 (MQ=255)                                                               cagAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCag                                                                                           <  2:279231/97‑1 (MQ=255)                                                                  agGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCg                                              >  1:6662/1‑139 (MQ=255)                                                                    gtggtTCTTCACCATCGGGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACGAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGc                                            >  1:284840/1‑139 (MQ=255)                                                                    gtggtTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGc                                            <  2:175446/139‑1 (MQ=255)                                                                         tcttcACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCa                                                                                            <  2:170239/86‑1 (MQ=255)                                                                         tcttcACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCa                                                                                            >  1:170239/1‑86 (MQ=255)                                                                         tcttcACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAgg                                       <  1:420849/139‑1 (MQ=255)                                                                                     cgTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATgg                           >  1:413287/1‑139 (MQ=255)                                                                                     cgTACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATgg                           >  2:119339/1‑139 (MQ=255)                                                                                       tACTTCGCGGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATGgcg                         <  2:445413/139‑1 (MQ=255)                                                                                            cgcgGCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATGgcgcgcgc                    >  1:132750/1‑139 (MQ=255)                                                                                                gCAAGTGGACATCGCCAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATGGCGCGCGCcaca                <  2:6662/139‑1 (MQ=255)                                                                                                              ccAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATGGCGCGCGCCACACCGTACTGTTCGaa  <  2:284840/139‑2 (MQ=255)                                                                                                              ccAGGGTATAGGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATGGCGCGCGCCACACCGTACTGTTCGAt  <  2:132750/139‑1 (MQ=255)                                                                                                                        |                                                                                                                                 CCGAGCGCCAGCAGAACAATACCGATAATGCCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTTAACCAGCGTAACGGTGGCAATAAAGCAGAGGATGAAGAAGCCCCACATGGTGGCGGTGCTGAGTGGCAGAGCGGCCCAGGTTTCAATGATGGCGCGCGCCACACCGTACTGTTCGAT  >  NC_000913/40532‑40779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑